Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Immunology
Ion Antohe, Mariana Pavel Tanasa, Angela Dascalescu, Catalin Danaila, Amalia Titieanu, Mihaela Zlei, Iuliu Ivanov, Adriana Sireteanu, Petru Cianga
Summary: This study found that patients with acute myeloid leukaemia (AML) who have low versus normal HLA-DR expression exhibit different profiles of MHC class II machinery molecules and B7 ligands, which are correlated with distinct ELN risk stratification.
Article
Immunology
Suzanne Bezstarosti, Cynthia S. M. Kramer, Marry E. I. Franke-van Dijk, Manon Vergunst, Kim H. Bakker, Merve Uyar-Mercankaya, Rico Buchli, Dave L. Roelen, Johan W. de Fijter, Frans H. J. Claas, Sebastiaan Heidt
Summary: HLA-DQ donor-specific antibodies (DSA) play a significant role in renal transplantation, and this study focuses on isolating HLA-DQ specific memory B cells to generate monoclonal antibodies that can verify the recognition of eplets on HLA molecules. The findings provide valuable insights into the epitopes of HLA-DQ, contributing to the understanding of HLA-DQ alloantibody pathogenicity in transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Se-Jin Kim, Elham Karamooz
Summary: This review discusses the current understanding of MR1 and HLA-E antigen presentation in the context of Mycobacterium tuberculosis infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Thuja Meurer, Pietro Crivello, Maximilian Metzing, Michel Kester, Dominik A. Megger, Weiqiang Chen, Peter A. van Veelen, Peter van Balen, Astrid M. Westendorf, Georg Homa, Sophia E. Layer, Amin T. Turki, Marieke Griffioen, Peter A. Horn, Barbara Sitek, Dietrich W. Beelen, J. H. Frederik Falkenburg, Esteban Arrieta-Bolanos, Katharina Fleischhauer
Summary: Research revealed that permissive HLA-DPB1 mismatches in hematopoietic cell transplantation have higher peptide repertoire overlaps and lower frequency and diversity of alloreactive TCR beta clonotypes in healthy individuals and transplanted patients compared to nonpermissive mismatches. The permissiveness can be reversed by peptide editor HLA-DM or its antagonist, HLA-DO, through significant broadening of the peptide repertoire. This highlights the key role of HLA-DM in harnessing T-cell alloreactivity to HLA-DP, making it a potential novel target for cellular and immunotherapy of leukemia.
Article
Biology
Julia Bauza-Martinez, Albert J. R. Heck, Wei Wu
Summary: Bauza-Martinez et al. analyzed the HLA-I peptide ligandome in extracellular vesicles (EVs) from an EBV-immortalized B cell line, JY, and compare HLA-I-binding peptides recovered from EVs to those recovered from whole-cells. The authors report an enrichment for HLA-B-binding peptides and for post-translationally modified cysteinylated peptides in EVs as compared to whole-cells.
COMMUNICATIONS BIOLOGY
(2021)
Article
Immunology
Paula Ruibal, Ian Derksen, Marjolein van Wolfswinkel, Linda Voogd, Kees L. M. C. Franken, Angela F. El Hebieshy, Thorbald van Hall, Tom A. W. Schoufour, Ruud H. Wijdeven, Tom H. M. Ottenhoff, Ferenc A. Scheeren, Simone A. Joosten
Summary: There is growing interest in HLA-E-restricted T-cell responses as a potential vaccination target. This study developed a method for efficient peptide thermal exchange on HLA-E monomers and multimers, allowing high-throughput production. The optimized conditions for detection and analysis of peptide-specific T cells revealed new insights into the interaction between HLA-E and NKG2A/CD94 receptors.
Article
Immunology
Lucy C. Sullivan, Thi H. O. Nguyen, Christopher M. Harpur, Sanda Stankovic, Abbie R. Kanagarajah, Marios Koutsakos, Philippa M. Saunders, Zhangying Cai, James A. Gray, Jacqueline M. L. Widjaja, Jie Lin, Gabriella Pietra, Maria Cristina Mingari, Lorenzo Moretta, Jerome Samir, Fabio Luciani, Glen P. Westall, Karl J. Malmberg, Katherine Kedzierska, Andrew G. Brooks
Summary: Human cytomegalovirus (CMV) infection can stimulate strong HLA-E-restricted CD8(+) T cell responses, with T cells recognizing UL40 peptide with high affinity but showing lower cytokine response. These T cells may avoid self-reactivity by expressing inhibitory natural killer cell receptors KIR2DL1 and KIR2DL2/L3, while T cells with lower-affinity TCRs express the activating receptor NKG2C.
SCIENCE IMMUNOLOGY
(2021)
Review
Biochemical Research Methods
Hannah B. Taylor, Susan Klaeger, Karl R. Clauser, Siranush Sarkizova, Shira Weingarten-Gabbay, Daniel B. Graham, Steven A. Carr, Jennifer G. Abelin
Summary: Immunotherapies have emerged as a way to treat diseases by selectively modulating a patient's immune response, but the challenge remains in selecting appropriate targets. HLA-II peptides are considered potential immunotherapy targets and biomarkers due to their role in activating CD4+ T cells and regulating B cell function. Systematic HLA-II peptidomics and multiomic analyses are needed to understand the molecular basis of HLA-II antigen presentation in disease progression and treatment.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Immunology
Kathryn A. K. Finton, Peter B. Rupert, Della J. Friend, Ana Dinca, Erica S. Lovelace, Matthew Buerger, Domnita V. Rusnac, Ulysses Foote-McNabb, William Chour, James R. Heath, Jean S. Campbell, Robert H. Pierce, Roland K. Strong
Summary: MHC class I single-chain trimer molecules are widely used in research, but caution is needed when selecting designs for studying peptides of different lengths. Predictions of peptide binding often do not match experimental results, and yields and stabilities vary with construct design. New reagents have been developed to improve the crystallizability of these proteins and novel modes of peptide presentation have been confirmed.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Robert Parker, Thomas Partridge, Catherine Wormald, Rebeca Kawahara, Victoria Stalls, Maria Aggelakopoulou, Jimmy Parker, Rebecca Powell Doherty, Yoanna Ariosa Morejon, Esther Lee, Kevin Saunders, Barton F. Haynes, Priyamvada Acharya, Morten Thaysen-Andersen, Persephone Borrow, Nicola Ternette
Summary: This study profiles the repertoire of HLA-DR-bound peptides presented by monocyte-derived dendritic cells pulsed with SARS-CoV-2 spike protein. It identifies 209 unique peptide sequences mapping to various sites on the S protein, with substantial trimming of glycan residues observed on the bound peptides. The study also highlights the receptor-binding motif in S1 as a HLA-DR-binding peptide-rich region and identifies potential target peptides in S2 for cross-protective vaccine-elicited responses.
Review
Immunology
Danillo G. Augusto, Jill A. Hollenbach
Summary: The extraordinary variation of HLA molecules is critical for the immune response in COVID-19, but still not fully understood. Studies have found associations between HLA variants and different outcomes of COVID-19, as well as differential immune responses against SARS-CoV-2. Mutations in viral strains, particularly in the Spike protein, affect the affinity between mutant peptides and HLA molecules. Understanding the impact of this variation on T-cell responses is crucial for comprehending the immunogenic mechanisms.
CURRENT OPINION IN IMMUNOLOGY
(2022)
Article
Immunology
You-Seok Hyun, Hyeong-A Jo, Yong-Hun Lee, Sun-Mi Kim, In-Cheol Baek, Hyun-Jung Sohn, Hyun-Il Cho, Tai-Gyu Kim
Summary: Within an individual, CD4(+) T cell responses are predominantly restricted by a limited number of HLA class II allotypes, with specific highly responsive alleles identified in each HLA-DR, -DQ, and -DP locus.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Natasha Salame, Jean-Pierre Bikorimana, Nehme El-Hachem, Wael Saad, Mazen Kurdi, Jing Zhao, Nicoletta Eliopoulos, Riam Shammaa, Moutih Rafei
Summary: The study reveals that MSCs can be chemically induced to become antigen-presenting cells using UM171a, which triggers the production of reactive oxygen species and promotes antigen cross-presentation. Therapeutic vaccination using UM171a-treated MSCs shows promising results in controlling tumor growth and improving survival rates in mice.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Yuki Uchihara, Tiara Bunga Mayang Permata, Hiro Sato, Reika Kawabata-Iwakawa, Sayako Katada, Wenchao Gu, Sangeeta Kakoti, Motohiro Yamauchi, Reona Kato, Soehartati Gondhowiardjo, Naoki Hosen, Takaaki Yasuhara, Atsushi Shibata
Summary: This study reveals that DNA damage signaling can produce immunogenic antigens by utilizing the machinery of PRT/NMD. DNA damage upregulates HLA class I antigen presentation, and antigen production is essential. Additionally, the pioneer translation factor CBP20, which initiates nonsense-mediated mRNA decay, plays a critical role in antigen production.
Article
Immunology
Zemin Zhou, Eduardo Reyes-Vargas, Hernando Escobar, Kuan Y. Chang, Adam P. Barker, Alan L. Rockwood, Julio C. Delgado, Xiao He, Peter E. Jensen
EUROPEAN JOURNAL OF IMMUNOLOGY
(2017)
Article
Medicine, Research & Experimental
Peng Zhao, Djordje Atanackovic, Shuyun Dong, Hideo Yagita, Xiao He, Mingnan Chen
MOLECULAR PHARMACEUTICS
(2017)
Article
Nanoscience & Nanotechnology
Lan Li, Chengke Luo, Zhenwei Song, Eduardo Reyes-Vargas, Fred Clayton, Jufang Huang, Peter Jensen, Xinjian Chen
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2018)
Article
Medicine, Research & Experimental
Peng Wang, Peng Zhao, Shuyun Dong, Tiefeng Xu, Xiao He, Mingnan Chen
Article
Engineering, Biomedical
Peng Wang, Shuyun Dong, Peng Zhao, Xiao He, Mingnan Chen
Article
Engineering, Biomedical
Peng Zhao, Peng Wang, Shuyun Dong, Zemin Zhou, Yanguang Cao, Hideo Yagita, Xiao He, Song Guo Zheng, Simon J. Fisher, Robert S. Fujinami, Mingnan Chen
NATURE BIOMEDICAL ENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Eduardo Reyes-Vargas, Adam P. Barker, Zemin Zhou, Xiao He, Peter E. Jensen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
Hemant R. Joshi, Harry R. Hill, Zemin Zhou, Xiao He, Karl V. Voelkerding, Attila Kumanovics
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Allergy
Hemant R. Joshi, Harry R. Hill, Julie Asch, Rebecca L. Margraf, Emily Coonrod, Jacob Durtschi, Qin Zhou, Xiao He, Karl V. Voelkerding, Attila Kumanovics
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Heejoo Kim, Jelena Perovanovic, Arvind Shakya, Zuolian Shen, Cody N. German, Andrea Ibarra, Jillian L. Jafek, Nai-Pin Lin, Brian D. Evavold, Danny H-C Chou, Peter E. Jensen, Xiao He, Dean Tantin
Summary: Loss of T cell-specific OCA-B protects mice from spontaneous autoimmune diabetes by reducing CD8(+) T cell receptor specificities associated with diabetes pathogenesis. The protective effect is recapitulated using autoantigen-specific NY8.3 mice but diminished in monoclonal models specific to artificial or neoantigens. Rational design of membrane-penetrating OCA-B peptide inhibitors normalizes glucose levels and reduces T cell infiltration and proinflammatory cytokine expression in newly diabetic NOD mice.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Hematology
Li Guo, Sikui Shen, Jesse W. Rowley, Neal D. Tolley, Wenwen Jia, Bhanu Kanth Manne, Kyra N. McComas, Ben Bolingbroke, Yasuhiro Kosaka, Krystin Krauel, Frederik Denorme, Shancy P. Jacob, Alicia S. Eustes, Robert A. Campbell, Elizabeth A. Middleton, Xiao He, Samuel M. Brown, Craig N. Morrell, Andrew S. Weyrich, Matthew T. Rondina
Summary: The study revealed that during sepsis, platelets upregulate antigen cross-presentation through MHC-I, interacting with antigen-specific CD8(+) T cells and regulating CD8(+) T-cell numbers, functional responses, and outcomes.
Article
Biochemistry & Molecular Biology
Lauren Girard, Kithsiri Herath, Hernando Escobar, Renate Reimschuessel, Olgica Ceric, Hiranthi Jayasuriya
Summary: FDA's CVM has been investigating reports of pets becoming ill after consuming jerky pet treats since 2007, with renal failure accounting for 30% of cases. Jerky pet treats containing glycerin may contain contaminants such as 3-MCPDEs and GEs, posing food safety concerns. Therefore, there is a need to develop a screening method to detect these substances in glycerin.