Article
Biochemical Research Methods
P. Jake Violi, P. David Bishop, P. Matthew Padula, T. Mika Westerhausen, J. Kenneth Rodgers
Summary: Developing LC-MS/MS methods for detecting native amino acids can be challenging, leading many scientists to derivatise amino acids to improve sensitivity. Derivatisation offers benefits such as reduced signal-to-noise ratios and more efficient ionisation, but it can also be costly and time-consuming. This study aimed to enhance the detection sensitivity of 20 protein amino acids using HILIC-MS/MS.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Applied
Ran Xin, Meng Dong, Yu-Ying Zhang, Xu-Hui Huang, Xiu-Ping Dong, Lei Qin
Summary: In this study, a rapid and reliable method based on HILIC-MS/MS was established for the simultaneous quantification and validation of 51 taste-active compounds in different food samples. The method showed good performance and successfully determined the compounds in 25 food samples.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Medical Laboratory Technology
Lavinia Santucci, Sara Lomuscio, Aniello Primiano, Riccardo Calvani, Silvia Persichilli, Federica Iavarone, Anna Picca, Francesca Canu, Andrea Urbani, Jacopo Gervasoni
Summary: This study aimed to develop a rapid method for the simultaneous determination of arginine and its metabolites in human plasma. A simple deproteinization procedure and hydrophilic interaction liquid chromatography were used for the pre-analytical and chromatographic separation, respectively. The method showed good recovery, precision, and stability, making it suitable for both research and clinical routine use.
CLINICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
Liuqing Zhao, Xiaodong Zhao, Yaping Xu, Xiaowei Liu, Jingran Zhang, Zeying He
Summary: A sensitive and reliable method for simultaneous determination of 49 amino acids, B vitamins, flavonoids, and phenolic acids in vegetables was developed and validated. Purple cabbage, broccoli, and red lettuce were found to have the highest concentrations of free amino acids, B vitamins, and phenolic compounds among the 26 commonly consumed vegetables.
Article
Chemistry, Applied
Jinping Wang, Junmei Liu, Afiya John, Yueming Jiang, Hong Zhu, Bao Yang, Lingrong Wen
Summary: This study prepared walnut protein hydrolysate (WPH) by enzymatic hydrolysis and analyzed its peptide sequence. Forty eight bioactive peptides were identified and antioxidant activities were detected for WPH. Among them, QGRPWG showed the highest antioxidant activity and AYNIPVNIAR possessed the strongest protective effect against H2O2-induced oxidative stress. These findings contribute to the recognition of walnut peptides as nutraceuticals.
Article
Biochemical Research Methods
E. Boutou, C. Virgiliou, A. K. Seliniotaki, M. Lithoxopoulou, A. Mataftsi, N. Ziakas, E. Diamanti, N. Raikos, H. Gika
Summary: A sensitive and accurate HILIC-MS/MS method was developed to determine the concentration of phenylephrine in DBS samples from preterm infants after ocular administration. The method involved extracting the analyte using methanol - acetonitrile and separating it on an ACQUITY UPLC BEH AMIDE column. Detection was achieved using electrospray ionization.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Olga Deda, Olga Begou, Helen Gika, Georgios Theodoridis, Agapios Agapiou
Summary: Carob is an economically important crop in the Mediterranean region, and its products have been found to have beneficial effects on health. In this study, the sample preparation of carob syrup and powder was optimized for metabolomics-based analysis, and the results showed that a pH-adjusted basic aqueous propanol solution and an acidic aqueous acetonitrile solution provided the best results for syrup and powder, respectively.
Article
Biochemical Research Methods
Zhengyuan Shi, Chunjing Yang, Xiqiao Xu, Wanshui Wu, Dechun Jiang, Dan Yan
Summary: By analyzing plasma samples of children with medulloblastoma and comparing them with samples from children with other intracranial tumors, this study identified differential metabolites and metabolic pathways. The results showed significant differences in glycerophospholipid and alpha-linolenic acid metabolism pathways, as well as amino acid metabolism pathways (phenylalanine, tyrosine, and tryptophan biosynthesis) between medulloblastoma patients and other tumor patients. These findings provide a basis for early diagnosis, as well as identifying new therapeutic targets and strategies for medulloblastoma.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Joanna Kaszuba, Ireneusz Kapusta, Zuzanna Posadzka
Summary: This study used UPLC-PDA-MS/MS to determine the profile of phenolic acids in triticale grain of selected Polish cultivars. Ferulic acid was found to be the main component, with differences in the composition and content of phenolic acids observed in flour and bread.
Article
Agriculture, Multidisciplinary
Jing Sun, Haoan Zhao, Fanhua Wu, Min Zhu, Ying Zhang, Ni Cheng, Xiaofeng Xue, Liming Wu, Wei Cao
Summary: This study analyzed the different metabolites of honey samples at different stages using a metabolomics approach, revealing a distinct metabolic profile in mature honey compared to immature honey. Specific fatty acids, such as decenedioic acid, were identified as potential markers for discriminating between immature and mature honey. Additionally, mature honey showed higher concentrations of fatty acids, particularly three fatty acids that were significantly richer in mature honey compared to immature honey.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Yuan Yuan, Jinle Xiang, Bailiang Zheng, Junjie Sun, Denglin Luo, Peiyan Li, Jinling Fan
Summary: The profile of phenolic compounds and antioxidant activity of proso millets were studied, revealing the presence of diverse phenolic derivatives, some of which were reported for the first time in proso millets. These compounds contributed to the antioxidant properties of proso millets, making them viable functional food ingredients.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Ruixue Ding, Mohan Li, Yiting Zou, Yiran Wang, Chunyue Yan, Haibing Zhang, Rina Wu, Junrui Wu
Summary: This study explored the quality indicators and metabolomic analysis of yogurts fermented using strict anaerobic and normal fermentation methods. The results showed that strict anaerobic fermentation improved the growth and acidification of bacteria, increased the sourness, umami, and richness, and changed the levels of amino acids and fatty acids in yogurts.
Article
Environmental Sciences
Ying-qiang Li, Ze-hua Liu, Sa Chen, Ying-juan Wu, Jing-tang Liang, Zhi Dang, Yun Liu
Summary: A trace determination method for 15 free amino acids in drinking source water was developed using solid-phase extraction coupled with ultra-high pressure liquid chromatography tandem mass spectrometry. The method showed excellent linearity, low limits of detection, and good recoveries, making it suitable for the analysis of this type of sample. The top five amino acids found in the drinking source water samples were serine, glycine, leucine, alanine, and isoleucine.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Analytical
Xiaona Li, Zhini Liu, Zhuo Li, Xin Xiong, Xianhua Zhang, Changqing Yang, Libo Zhao, Rongsheng Zhao
Summary: A novel LC-MS method was developed for simultaneous quantification of 16 purine metabolites in human plasma and urine. The method showed good linearity, low quantitation limits, and stable recovery and matrix effects. The method was successfully applied to compare the plasma and urine samples of patients without and with acute kidney injury (AKI), revealing potential upregulation of certain purine intermediates in AKI patients, indicating an association with inflammatory responses.
Article
Multidisciplinary Sciences
Mayy M. Mostafa, Mohamed A. Farag
Summary: This study analyzed the metabolites of different cultivars of Prunus mahaleb L. It was found that white cultivars were abundant in phenolic acids and amino acids, while red cultivars and roasted seeds were rich in organic acids and nitrogenous compounds.
Article
Biochemical Research Methods
Thomas Meikopoulos, Olga Deda, Efstratios Karagiannidis, Georgios Sianos, Georgios Theodoridis, Helen Gika
Summary: In this study, a rapid quantitation method for acylcarnitines in human serum was developed and validated. The method was successfully applied in the analysis of samples from patients with coronary artery disease.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Dimitra Diamantidou, Orthodoxia Mastrogianni, Emmanouil Tsochatzis, Georgios Theodoridis, Nikolaos Raikos, Helen Gika, Stavros Kalogiannis
Summary: A simple, fast, and sensitive method was developed for the analysis of cyclic oligomers in food packaging materials. The method allows for the determination of these oligomers in blood samples, providing a useful tool to assess potential hazards and health risks associated with food consumption.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Andreas S. Papazoglou, Dimitrios Moysidis, Christos Tsagkaris, Ioannis Vouloagkas, Efstratios Karagiannidis, Anastasios Kartas, Nikolaos Vlachopoulos, Georgios Konstantinou, Georgios Sofidis, Nikolaos Stalikas, Eleftherios Panteris, Olga Deda, Nikolaos Otountzidis, Haralambos Karvounis, Helen Gika, Georgios Theodoridis, Georgios Sianos
Summary: Religious fasting has a significant impact on the metabolic and hematological profiles of Orthodox and Muslim participants, affecting parameters like fasting glucose, cholesterol, hemoglobin, iron, and calcium. However, further studies with larger sample sizes and longer follow-ups are needed to confirm the effects of fasting on human health.
EUROPEAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Biochemical Research Methods
E. Boutou, C. Virgiliou, A. K. Seliniotaki, M. Lithoxopoulou, A. Mataftsi, N. Ziakas, E. Diamanti, N. Raikos, H. Gika
Summary: A sensitive and accurate HILIC-MS/MS method was developed to determine the concentration of phenylephrine in DBS samples from preterm infants after ocular administration. The method involved extracting the analyte using methanol - acetonitrile and separating it on an ACQUITY UPLC BEH AMIDE column. Detection was achieved using electrospray ionization.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Amvrosios Orfanidis, Helen Gika, Georgios Theodoridis, Anastasia Chrysovalantou Chatziioannou, Nikolaos Raikos
Summary: The present study conducted a thorough research on the stability of drugs of abuse and pharmaceuticals over a period of 12 months. The results showed that the presence of formalin had a catalytic effect on the stability of the drugs, leading to a significant decrease in the number of detected compounds over time.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Nikolaos Fragakis, Antonios P. Antoniadis, Melani Sotiriadou, Christina Virgiliou, Iris Ballauri, Helen G. Gika, Dimitrios Bougiouklis, Spyros Gerou, Charalampos Lazaridis, Stavros Vergopoulos, Constantinos Bakogiannis, Georgios Giannopoulos, Georgios A. Theodoridis, Christodoulos E. Papadopoulos, Theodoros D. Karamitsos, Vassilios Vassilikos
Summary: In syncopal patients without underlying structural disease, the levels of ADP are associated with the clinical presentation of neurally mediated syncope and the outcomes of HUTT and ADT. NPS patients with positive HUTT show excessive ADP release at the time of syncope, explaining the lack of prodromes in this form of syncope.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2022)
Article
Medicine, General & Internal
Agathi Thomaidou, Olga Deda, Olga Begou, Artemis Lioupi, Angeliki Kontou, Helen Gika, Eleni Agakidou, Georgios Theodoridis, Kosmas Sarafidis
Summary: This study evaluated the metabolic profile of neonates with late-onset sepsis (LOS) and necrotizing enterocolitis (NEC). The results revealed significant differences in the metabolic profile of neonates with LOS or NEC compared to controls, suggesting that certain phospholipids and their derivatives could be used as biomarkers for early detection of these diseases.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Eleftherios Panteris, Olga Deda, Andreas S. Papazoglou, Efstratios Karagiannidis, Theodoros Liapikos, Olga Begou, Thomas Meikopoulos, Thomai Mouskeftara, Georgios Sofidis, Georgios Sianos, Georgios Theodoridis, Helen Gika
Summary: Developing risk assessment tools for CAD prediction is challenging. This study developed a machine learning algorithm to predict the severity of CAD based on metabolic and clinical data. The algorithm incorporated clinical and metabolic features to estimate the pre-test likelihood of obstructive CAD.
Correction
Microbiology
Charalampos Antachopoulos, Anastasia Geladari, Cornelia B. Landersdorfer, Eleni Volakli, Stavroula Ilia, Evangelos Gikas, Helen Gika, Maria Sdougka, Roger L. Nation, Emmanuel Roilides
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemical Research Methods
Dimitra Diamantidou, Ioannis Sampsonidis, Theodoros Liapikos, Helen Gika, Georgios Theodoridis
Summary: Untargeted metabolomic studies require selective and efficient analytical methodologies such as reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) to obtain extensive metabolic information. This study compared the performance of five different chromatographic columns and proposed a column scoring approach to assist in the selection of a suitable stationary phase for metabolite analysis. RPLC methods showed better metabolic library coverage, while the zicHILIC stationary phase eluted a larger number of compounds. The study highlights the importance of choosing the most suitable configuration for analyzing different metabolic classes.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Alexandros Nakas, Georgia Giannarelli, Ioannis Fotopoulos, Eirini Chainoglou, Aikaterini Peperidou, Konstantinos N. N. Kontogiannopoulos, Artemis Tsiaprazi-Stamou, Vasilios Varsamis, Helen Gika, Dimitra Hadjipavlou-Litina, Andreana N. N. Assimopoulou
Summary: The aim of this study was to optimize the distillation process of oregano essential oil to maximize the yield of bioactive compounds and evaluate their antioxidant activity. Results showed that time was the most influential factor affecting the yield, and lower pressure, shorter time, and higher temperature were beneficial for antioxidant activity.
Article
Infectious Diseases
Maria Kipourou, Olga Begou, Katerina Manika, Georgios Ismailos, Paschalina Kontou, Georgia Pitsiou, Helen Gika, Ioannis Kioumis
Summary: This study evaluated the pharmacokinetic profile of tigecycline in plasma and its penetration to sputum in moderately ill patients with an infectious acute exacerbation of chronic obstructive pulmonary disease (COPD). The results showed that tigecycline has sufficient lung penetration in pulmonary infections of moderate severity and has clinical effectiveness against commonly causative bacteria in COPD exacerbations.
Article
Chemistry, Analytical
Georgios Theodoridis, Helen Gika, Daniel Raftery, Royston Goodacre, Robert S. Plumb, Ian D. Wilson
Summary: Metabolite identification is a major challenge in contemporary metabolomics research, particularly in liquid chromatography-mass spectrometry technology. This article categorizes the errors into two types: biologically implausible identities and identities that do not match the chromatographic and physicochemical properties of the proposed molecule. It discusses the issue, presents examples, and proposes measures for improvement.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Artemis Lioupi, Maria Marinaki, Christina Virgiliou, Olga Begou, Helen Gika, Ian Wilson, Georgios Theodoridis
Summary: Metabolomics is an interdisciplinary field with applications in various areas, involving the analysis of metabolites in pathways such as glycolysis, amino acid metabolism, and the Krebs cycle. The determination of polar metabolites poses major challenges for chromatographic analysis, and existing methods are unable to comprehensively profile the polar metabolome. This review summarizes current approaches, advances, and trends in using liquid chromatography/mass spectrometry to address the instrumental and intellectual challenges involved in mapping the polar metabolome.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ioannis Sampsonidis, Maria Marinaki, Anastasia Pesiridou, Helen Gika, Georgios Theodoridis, Nektarios Siachos, Georgios Arsenos, Stavros Kalogiannis
Summary: In this study, a simple HILIC-MS/MS method was developed and validated for the individual determination of tau-MH and pi-MH in a large cohort of blood plasma samples from dairy cows. The method achieved separate determination of the isomers through a mass spectrometry fragment ion study and optimized chromatographic conditions. The developed method provided good linearity and precision for the determination of tau-MH and pi-MH in bovine plasma. Overall, this method enables efficient and economic-friendly determination of the isomers, suitable for large cohort bovine studies.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)