Article
Pharmacology & Pharmacy
Sarit S. Sivan, Iris Bonstein, Yariv N. Marmor, Gadi Pelled, Zulma Gazit, Michal Amit
Summary: This study presents a novel method for encapsulating human bone marrow stem cells in small-sized microspheres using one-step emulsification by internal gelation. The resulted microspheres demonstrated high viability and integrity, and showed long-lasting viability and biocompatibility in an intervertebral disc model.
Article
Multidisciplinary Sciences
Veronica Crisostomo, Claudia Baez-Diaz, Virginia Blanco-Blazquez, Veronica Alvarez, Esther Lopez-Nieto, Juan Maestre, Antoni Bayes-Genis, Carolina Galvez-Monton, Javier G. Casado, Francisco M. Sanchez-Margallo
Summary: Epicardial administration of CDCs or their EVs is safe and technically feasible 3 days after experimental myocardial infarction in swine, but does not appear to have any beneficial effect on cardiac function. The results do not support the clinical translation of these therapies as implemented in this study.
SCIENTIFIC REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Toshikazu Sano, Tatsuo Ito, Shuta Ishigami, Srinivas Bandaru, Shunji Sano
Summary: This study aimed to enhance the cardiomyogenic efficiency of cardiosphere-derived cells (CDCs) using an intrinsic activation system, resulting in improved regenerative potential and functional benefits in a rat model of acute myocardial infarction. The CRISPR/dCas9 system showed promise in regulating the activation of Tnnt2 gene within stem cells, which could lead to better therapeutic outcomes for patients with ischemic heart disease.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2022)
Article
Cardiac & Cardiovascular Systems
Klaus Neef, Florian Drey, Vera Lepperhof, Thorsten Wahlers, Jurgen Hescheler, Yeong-Hoon Choi, Tomo Saric
Summary: Co-transplantation of iPS-CM and MSC improves cardiac regeneration after myocardial damage, demonstrating the potential of combining multiple cell types for increasing the efficacy of future cardiac cell therapies. MSCs enhance the retention of iPS-CM and result in a significantly higher left ventricular ejection fraction (LVEF) when transplanted together.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Review
Cell Biology
Yuchang Liu, Minrui Wang, Yang Yu, Chunhong Li, Chunxiang Zhang
Summary: Acute myocardial infarction, characterized by irreversible cardiomyocyte death and restricted blood supply, is the leading cause of death in coronary heart disease. Conventional reperfusion therapy can worsen myocardial injury. Stem cell therapy using mesenchymal stem cells (MSCs) has emerged as a promising approach for cardiac repair and function improvement. MSCs may exert their effects by secreting exosomes containing therapeutically active RNA, proteins, and lipids. Analyzing these vesicles can provide valuable insights into myocardial infarction pathology and guide the development of new treatments.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Medicine, Research & Experimental
Si-Jia Sun, Wing-Hon Lai, Yu Jiang, Zhe Zhen, Rui Wei, Qizhou Lian, Song-Yan Liao, Hung-Fat Tse
Summary: Pre-transplantation systemic intravenous administration of hiPSC-MSCs induced immunomodulation and facilitated the survival of intramyocardially transplanted cells to improve cardiac function in MI.
Article
Cell & Tissue Engineering
Sujitha Thavapalachandran, Thi Yen Loan Le, Sara Romanazzo, Fairooj N. Rashid, Masahito Ogawa, Kristopher A. Kilian, Paula Brown, Jim Pouliopoulos, Anthony M. Barry, Peter Fahmy, Kilian Kelly, Eddy Kizana, James J. H. Chong
Summary: The study demonstrates that iPSC-MSCs (Cymerus MSCs) provide better therapeutic effects for myocardial ischemia-reperfusion injury in a rodent model compared to conventional BM-MSCs, with safety and scalability for potential off-the-shelf stem cell therapy for cardiac repair.
Article
Cell Biology
Asim Cengiz Akbulut, Grzegorz B. Wasilewski, Nikolas Rapp, Francesco Forin, Heike Singer, Katrin J. Czogalla-Nitsche, Leon J. Schurgers
Summary: This study generated mature osteoblasts from iPSCs using a stable expandable MSC intermediate, and investigated the effects of MK-7 on cell differentiation. The findings suggest that MK-7 promotes osteoblast maturation and increases osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Huifeng Zheng, Xiaoting Liang, Qian Han, Zhuang Shao, Yuxiao Zhang, Linli Shi, Yimei Hong, Weifeng Li, Cong Mai, Qingwen Mo, Qingling Fu, Xiaoxue Ma, Fang Lin, Mimi Li, Bei Hu, Xin Li, Yuelin Zhang
Summary: The study demonstrates that Hemin-pretreated MSC-derived exosomes are more effective in treating myocardial infarction compared to regular MSC-derived exosomes, with exosomal miR-183-5p playing a key role in inhibiting cardiomyocyte senescence through regulation of the HMGB1/ERK pathway.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Immunology
Kento Homma, Nikolay Bazhanov, Kazuki Hashimoto, Masaru Shimizu, Thomas Heathman, Qi Hao, Ranjana Nawgiri, Vidarshi Muthukumarana, Jae Woo Lee, Donald S. S. Prough, Perenlei Enkhbaatar
Summary: The pathogenesis of sepsis is an imbalance between pro-inflammatory and anti-inflammatory responses. MSC-derived extracellular vesicles (EVs) have shown beneficial effects for the treatment of acute lung injury (ALI) and sepsis. However, the present study demonstrated that EVs isolated from bone marrow-derived MSCs failed to attenuate the severity of multiorgan dysfunctions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Jianyong Xu, Huimei Wu, Zhigang Mai, Junbo Yi, Xianqi Wang, Lingyun Li, Zhong Huang
Summary: In this study, we aimed to establish a safer strategy for generating induced cardiac progenitor-like cells (iCS) through comparing four transgene-free reprogramming approaches. The self-replicative RNA method was found to produce more iCS with cardiomyocyte differentiation ability and therapeutic effects comparable to those generated with retrovirus. Additionally, the CXCR4 positive subpopulation of iCS derived cells delivered by intravenous injection showed similar therapeutic effects with intramyocardial injection in the mouse model of MI, representing a safer delivery approach.
CELL PROLIFERATION
(2021)
Article
Engineering, Environmental
Junlang Li, Yongbo Lv, Dashuai Zhu, Xuan Mei, Ke Huang, Xianyun Wang, Zhenhua Li, Sichen Zhang, Shiqi Hu, Kristen D. Popowski, Ke Cheng, Juan Wang
Summary: A comparison between intramyocardial (IM) injection and intrapericardial cavity (IPC) injection of adult stem cells in hydrogel showed that IPC injection led to better retention and therapeutic benefits, resulting in significant improvement in cardiac function. Furthermore, IPC delivery demonstrated better cellular proliferation, less apoptosis, and improved vascular regeneration in the myocardium. Additionally, IPC injections led to higher rates of MSC-exosome absorption by heart cells, indicating more extensive paracrine activity and showing promise as a solution for the low retention issue of MSCs in the infarcted heart.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biotechnology & Applied Microbiology
Jing Li, Yuting Tang, Leijing Yin, Xiaofang Lin, Zhengyang Luo, Shuxin Wang, Ludong Yuan, Pengfei Liang, Bimei Jiang
Summary: Myocardial infarction refers to irreversible cardiac function impairment caused by inadequate blood supply to the heart. Mesenchymal stem cell-derived exosomes play a crucial role in its treatment, primarily through their paracrine secretion of multiple factors. Despite the advantages of exosome therapy, maintaining stability and enhancing targeting effects remain challenging in clinical applications.
JOURNAL OF GENE MEDICINE
(2023)
Review
Urology & Nephrology
Vanesa Lucas-Cava, Francisco Miguel Sanchez-Margallo, Inigo Insausti-Gorbea, Fei Sun
Summary: This study assessed and compared the clinical efficacy and safety of prostatic urethral lift (PUL) and prostatic artery embolization (PAE) for the treatment of lower urinary tract symptoms (LUTS) in patients with benign prostatic hyperplasia (BPH) through a systematic review and network meta-analysis. The findings suggest that PUL and PAE have similar clinical efficacy and safety profiles in managing LUTS associated with BPH, although the quality of evidence is relatively low due to a limited number of randomized controlled trials (RCTs).
Article
Polymer Science
Federico Soria, Luna Martinez-Pla, Salvador D. Aznar-Cervantes, Julia E. de la Cruz, Tomas Fernandez, Daniel Perez-Fentes, Luis Llanes, Francisco Miguel Sanchez-Margallo
Summary: The study evaluated a new instillation technology using a biodegradable ureteral stent/scaffold coated with a silk fibroin matrix for controlled release of mitomycin C as an anti-cancer drug, which effectively decreased the cell viability in urothelial carcinoma cells.
Article
Biotechnology & Applied Microbiology
David Duran-Rey, Ricardo Brito-Pereira, Clarisse Ribeiro, Sylvie Ribeiro, Juan A. Sanchez-Margallo, Veronica Crisostomo, Igor Irastorza, Unai Silvan, Senentxu Lanceros-Mendez, Francisco M. Sanchez-Margallo
Summary: This study investigates the characterization and analysis of poly(vinylidene fluoride) (PVDF) structures prepared using different processing methods. The PVDF scaffolds exhibit good mechanical properties and biocompatibility, with variations in characteristics depending on the processing method used. However, the cell viability and proliferation are not affected by the different PVDF architectures. The topography of PVDF can influence the behavior of human umbilical vein endothelial cells (HUVECs) and may be used for the design of vascular implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Laura Paz-Artigas, Pilar Montero-Calle, Olalla Iglesias-Garcia, Manuel M. Mazo, Ignacio Ochoa, Jesus Ciriza
Summary: Myocardial ischaemia is a major cause of death worldwide. Animal models, although informative, lack the characteristics of human patients with ischemia and are time-consuming and expensive. In vitro models that mimic the human cardiac microenvironment during an ischaemic event can help develop new drugs, understand molecular events, and optimize therapeutic approaches. Current in vitro cardiac culture systems, from organoids to heart-on-chip platforms, can recreate ischaemic hallmarks, but still have limitations in fully recapitulating the complexity of clinical situations.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biology
Maria Pulido, Maria Angeles de Pedro, Veronica Alvarez, Ana Maria Marchena, Virginia Blanco-Blazquez, Claudia Baez-Diaz, Veronica Crisostomo, Javier G. Casado, Francisco Miguel Sanchez-Margallo, Esther Lopez
Summary: In this study, a comparative transcriptome analysis was conducted to explore the differences in gene expression between ischemic and remote myocardium after acute myocardial infarction. The results identified impaired mitochondrion-related biological processes in the infarcted area, as well as up-regulated immune system process-related genes in the remote tissue. These findings provide valuable insights for the development of new treatments for acute myocardial infarction.
Article
Cardiac & Cardiovascular Systems
Virginia Blanco-Blazquez, Claudia Baez-Diaz, Francisco Miguel Sanchez-Margallo, Irene Gonzalez-Bueno, Helena Martin, Rebeca Blazquez, Javier G. G. Casado, Alejandra Uson, Julia Solares, Itziar Palacios, Rob Steendam, Veronica Crisostomo
Summary: The study evaluated the cardiac safety and effectiveness of intracoronary administration of HGF-loaded extended release microspheres in an acute myocardial infarction swine model. The results showed that the treatment with HGF-loaded microspheres did not improve cardiac function, reduce inflammation, or cardiac fibrosis.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2023)
Article
Multidisciplinary Sciences
Robert Wise, Reitze Rodseth, Ester Parraga-Ros, Rafael Latorre, Octavio Lopez Albors, Laura Correa-Martin, Francisco M. Sanchez-Margallo, Irma Eugenia Candanosa-Aranda, Jan Poelaert, Gregorio Castellanos, Manu L. N. G. Malbrain
Summary: This study investigated the pathophysiological effects of intra-abdominal hypertension using animal models. The results showed significant impacts on multiple organ systems, especially the cardiovascular, respiratory, renal, and gastrointestinal systems. The mechanical intestinal obstruction model had a more pronounced effect on metabolic and hematological parameters.
Article
Polymer Science
Jorge Loureiro, Sonia P. Miguel, Victor Galvan-Chacon, David Patrocinio, Jose Blas Pagador, Francisco M. Sanchez-Margallo, Maximiano P. Ribeiro, Paula Coutinho
Summary: Myocardial infarction is a common cardiovascular disease and the leading cause of death worldwide. A 3D printed hydrogel, made from natural polymers, has been developed for cardiac tissue regeneration. The hydrogel, consisting of Gellan gum and Konjac glucomannan, shows excellent printability and possesses proper mechanical properties. It promotes endothelial cells' proliferation and migration, enabling the repair of injured cardiac tissue. This study provides the potential for a simple, low-cost, and efficient cardiac tissue regeneration method using a 3D printed hydrogel for myocardial infarction treatment in the future.
Article
Biotechnology & Applied Microbiology
E. Fernandez-Carro, R. Salomon-Cambero, L. Armero, H. A. Castro-Abril, J. Ayensa-Jimenez, Miguel A. Martinez, I. Ochoa, C. Alcaine, I. Garcia, J. Ciriza
Summary: Tissue barrier permeability is crucial for the selective transport of substances across epithelial tissues. Microfluidic devices and organ-on-chip technologies are important for generating reliable data on permeability. An alternative stratified epithelia-on-chip model has been designed to analyze diffusion through stratified epithelium and predict the Stokes radius for unknown particles within a molecular size range.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2023)
Article
Imaging Science & Photographic Technology
Luisa F. Sanchez-Peralta, Ben Glover, Cristina L. Saratxaga, Juan Francisco Ortega-Moran, Scarlet Nazarian, Artzai Picon, J. Blas Pagador, Francisco M. Sanchez-Margallo
Summary: Colorectal cancer is a major cause of death worldwide, but early detection can greatly increase survival rates. Artificial intelligence and deep learning methods have been successfully applied to improve detection and localization of polyps in colonoscopy. However, there is a need for standardized comparison with clinical experts before their clinical validation.
JOURNAL OF IMAGING
(2023)
Article
Biochemical Research Methods
Sandra Gonzalez-Lana, Teodora Randelovic, Jesus Ciriza, Maria Lopez-Valdeolivas, Rosa Monge, Carlos Sanchez-Somolinos, Ignacio Ochoa
Summary: The tissue microenvironment is crucial for tissue homeostasis and disease progression, but there has been a lack of adequate biomimetic models for in vitro simulation. However, microfluidic technology has allowed the recreation of these complex microenvironments using hydrogels, cells, and microfluidic devices. Despite the advancements, there are limitations such as the collapse of 3D structures when contractile cells exert forces.
Article
Biochemistry & Molecular Biology
Maria Ageles de Pedro, Maria Pulido, Veronica alvarez, Federica Marinaro, Ana Maria Marchena, Francisco Miguel Sanchez-Margallo, Javier G. Casado, Esther Lopez
Summary: This study found that Menstrual blood-derived stromal cells (MenSCs) have a good survival capacity under acute hypoxia conditions and have potential applications in tissue regeneration through their secretome, making them a potential cell source for wound healing interventions.
MOLECULAR MEDICINE
(2023)
Review
Polymer Science
David Patrocinio, Victor Galvan-Chacon, J. Carlos Gomez-Blanco, Sonia P. Miguel, Jorge Loureiro, Maximiano P. Ribeiro, Paula Coutinho, J. Blas Pagador, Francisco M. Sanchez-Margallo
Summary: Tissue engineering focuses on developing 3D structures through bioprinting techniques using proteins and polysaccharides, but the rheological behaviors of these natural-based bioinks are the main challenge in the cell-laden printing process.
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
