Emerging Strategies in Polyethylene Terephthalate Hydrolase Research for Biorecycling

The research on polyethylene terephthalate (PET) hydrolyzing enzymes started in 2005; several studies are now nearing the objective of their application in biorecycling of PET, which is an urgent environmental issue. The thermostability of PET hydrolases must be higher than 70 degrees C, which has already been established by several thermophilic cutinases, as higher thermostability results in higher activity. Additionally, pretreatment of waste PET to more enzyme-attackable forms is necessary for PET biorecycling. This Minireview summarizes research on enzymatic PET hydrolysis from two viewpoints: 1) improvement of PET hydrolases by focusing on their thermostabilities by mutation of enzyme genes, their expression in several hosts, and their modifications; and 2) processing of waste PET to readily biodegradable forms. Finally, the outlook of PET biorecycling is described.

Automated summary

Research on PET hydrolyzing enzymes aims to increase thermostability for higher activity, as well as to pretreat waste PET for biodegradability. Improving enzyme thermostabilities through gene mutation, host expression, and modifications, as well as processing waste PET into more readily biodegradable forms, are crucial for achieving PET biorecycling.