Journal
CELLULAR IMMUNOLOGY
Volume 363, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2021.104342
Keywords
Multiple myeloma; Chimeric antigen receptor; Adoptive T cell therapy; B-cell maturation antigen
Categories
Funding
- Jiangxi Key Research and Development Program of China [20201BBG71009, 20171BBG70057]
- Shanghai HRAIN Biotechnology Co., Ltd.
Ask authors/readers for more resources
The study confirmed that CAR-T cells targeting BCMA can effectively eliminate multiple myeloma, and CAR-T cells co-expressing tEGFR have the potential for cellular ablation.
Background: Chimeric antigen receptor T cells (CAR-T) against B-cell maturation antigen (BCMA) has been used to treat multiple myeloma (MM). CAR-T cells co-expressing a truncated human EGFR (tEGFR) has been proposed for in vivo cell ablation. Methods: We designed and tested a novel anti-BCMA CAR. We transduced T cells with retroviral vectors encoding CAR and tEGFR. The anti-BCMA-CAR-transduced T cells were evaluated for the functions including cytokine production, proliferation, cytotoxicity, and in vivo tumor eradication of BCMA. Cetuximab was used for in vivo cell ablation. Results: The CAR-T cells could specifically recognize BCMA, and anti-BCMA CAR-T cells could exhibit interferon? and cytotoxicity specifically produced by BCMA and eradicate tumor in vivo. Cetuximab could mediate antibody-dependent cellular cytotoxicity and in vivo elimination. Conclusions: We confirm that BCMA is a suitable target for CAR- T cells and tEGFR is a effective tool for cellular ablation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available