Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 13, Pages 5606-5611Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c00335
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Funding
- SinoGerman Center for Research Promotion [C-0008]
- National Natural Science Foundation of China [21974101]
- Largescale Instrument and Equipment Sharing Foundation of Wuhan University
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This study introduced a DNAzyme walker for homogeneous and isothermal detection of enterovirus, which showed high sensitivity and easy operation.
When dealing with infectious pathogens, the risk of contamination or infection in the process of detecting them is nonnegligible. Separation-free detection will be beneficial in operation and safety. In this work, we proposed a DNAzyme walker for homogeneous and isothermal detection of enterovirus. The DNAzyme is divided into two inactivate subunits. When the subunit-conjugated antibody binds to the target virus, the activity of the DNAzyme recovers as a result of spatial proximity. The walker propels, and the fluorescence recovers. The final fluorescence intensity of the reaction mixture is related to the concentration of the target virus. The detection limit of this proposed method is 6.6 x 10(4) copies/mL for EV71 and 4.3 x 10(4) copies/mL for CVB3, respectively. Besides, this method was applied in detection of EV71 in clinical samples with a satisfactory result. The entire experiment is easy to operate, and the proposed method has great potential for practical use.
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