Article
Biochemistry & Molecular Biology
Andrew P. Bischer, Timothy M. Baran, Andrew P. Wojtovich
Summary: Environmental surveillance-mediated behavior in Caenorhabditis elegans integrates multiple cues and is coordinated by neurons through signaling cascades. Despite lacking eyes, C. elegans is able to perceive and react to the color blue. This study explains this color perception by showing that internally-generated reactive oxygen species (ROS), in response to light, add to exogenous sources of ROS. Multiple sub-threshold sources of ROS are integrated to coordinate behavioral responses with internal cues. Blue light affects C. elegans behavior through ROS generation by endogenous flavins, mediated by the neuronal gustatory photoreceptor like protein, LITE-1. Overall, this study demonstrates that ROS and LITE-1 play central roles in C. elegans foraging behavior through integration of multiple inputs, including light.
Article
Biochemistry & Molecular Biology
Daniil A. A. Chernyavskij, Olga Yu. Pletjushkina, Anastasia V. V. Kashtanova, Ivan I. I. Galkin, Anna Karpukhina, Boris V. V. Chernyak, Yegor S. S. Vassetzky, Ekaterina N. N. Popova
Summary: Many muscular pathologies involving oxidative stress and elevated TNF levels cause muscle protein catabolism and impair myogenesis. This study found that mitoROS play a role in this process by triggering and enhancing mitophagy during myogenesis. Treatment with TNF before myogenesis decreases myoblast fusion and MYH2 synthesis, but antioxidants and autophagy suppression can partially restore myogenesis.
Article
Plant Sciences
Adeola Folasade Ehigie, Peng Wei, Taotao Wei, Xiyun Yan, Olufunso O. Olorunsogo, Fiyinfoluwa Demilade Ojeniyi, Leonard Ona Ehigie
Summary: The study aimed to investigate the mechanism of cell death in breast and lung cancer cell lines treated with fractions derived from bitter melon extract. The results showed that the fractions reduced mitochondrial membrane potential and intracellular ATP levels, while increasing reactive oxygen species levels. However, they did not activate caspase-3 or release cytochrome c in the cancer cells, suggesting a different mechanism of cell death induction.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Renkai Li, Chengwen Zheng, Polly Ho-Ting Shiu, Panthakarn Rangsinth, Wen Wang, Yiu-Wa Kwan, Emily Sze-Wan Wong, Yanbo Zhang, Jingjing Li, George Pak-Heng Leung
Summary: Despite various therapeutic approaches, developing novel and more effective methods for colorectal cancer treatment is crucial due to its high fatality rate. The recent study explored the anticancer effects of GAR E, a bioactive xanthone found in mangosteen, and identified the involvement of the ROS/JNK signaling pathway in its mechanism of action. The experiments demonstrated that GAR E inhibited colony formation and wound healing in cancer cells, triggered ROS production, induced mitochondrial dysfunction and apoptosis, and caused cell cycle arrest. In vivo experiments also confirmed the antitumor effect of GAR E in a xenograft mouse model. These findings suggest that GAR E could be a potential chemotherapeutic agent for colorectal cancer treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Javier Florido, Cesar Rodriguez-Santana, Laura Martinez-Ruiz, Alba Lopez-Rodriguez, Dario Acuna-Castroviejo, Iryna Rusanova, Germaine Escames
Summary: ROS are highly reactive molecules that regulate important signaling pathways. Tumor cells have altered redox balance compared to normal cells, making them susceptible to anti-tumoral therapy targeting ROS levels and antioxidant system capacity. Melatonin has potential as an innovative cancer treatment, but its mechanism of action remains unclear, limiting its clinical applications.
Article
Biochemistry & Molecular Biology
James H. Schofield, Zachary T. Schafer
Summary: The relationship between mitophagy and ROS production is complex and not fully understood. This review discusses mtROS generation and their detrimental effects on cellular viability, along with the cellular defense mechanisms against oxidative stress. Furthermore, the prominent mechanisms governing mitophagy induction that bear on oxidative stress are explored.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Engineering, Environmental
Guoliang Chen, Mingxing Wang, Panpan Zhu, Guixue Wang, Tingzhang Hu
Summary: Pyraoxystrobin (SYP-3343), a newly-invented strobilurin fungicide, exhibits high toxicity to aquatic organisms, particularly in zebrafish embryos, through affecting cell cycle, mitochondrial membrane potential, and reactive oxygen species generation. This toxicity is dose- and time-dependent, inducing apoptosis in zebrafish embryos.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Immunology
Miranda D. Chavez, Hubert M. Tse
Summary: Mitochondrial dysfunction and oxidative stress are associated with T cell-mediated autoimmune diseases, and targeting metabolic pathways can inhibit autoreactive T cell activation. Increasing the requirements of Tregs for ROS and oxidative phosphorylation can promote self-tolerance and inhibit the activity of autoreactive T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li-Chiu Yang, Yu-Chao Chang, Kun-Lin Yeh, Fu-Mei Huang, Ni-Yu Su, Yu-Hsiang Kuan
Summary: This study investigated the protective mechanism of rutin in alleviating TEGDMA-induced toxicity in macrophages. Results showed that rutin substantially reduced cytotoxicity, apoptosis, and genotoxicity, and decreased oxidative stress and ROS generation caused by TEGDMA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chou-Yuan Ko, Po-Chang Shih, Po-Wei Huang, Yi-Hsin Lee, Yen-Fu Chen, Ming-Hong Tai, Chi-Hao Liu, Zhi-Hong Wen, Hsiao-Mei Kuo
Summary: Sinularin was found to effectively inhibit the growth of hepatocellular carcinoma SK-HEP-1 cells at a concentration of 10 μM, inducing DNA fragmentation and apoptosis, as well as affecting cell migration and colony formation. Additionally, it altered the expression levels of various proteins related to cell signaling and metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Namasthee Harris-Gauthier, Annika Traa, Abdelrahman AlOkda, Alibek Moldakozhayev, Ulrich Anglas, Sonja K. Soo, Jeremy M. Van Raamsdonk
Summary: Mild impairment of mitochondrial function can increase lifespan in genetic model organisms. The upregulation of genes involved in the mitochondrial thioredoxin system, mediated by the mitochondrial unfolded protein response, is specifically required for the longevity of mitochondrial mutants. The mitochondrial thioredoxin system also plays a role in stress resistance.
Article
Biochemistry & Molecular Biology
Barry Halliwell
Summary: The field of oxygen free radicals, antioxidants, and reactive oxygen species (ROS) has seen significant growth, with ROS playing important roles in living organisms. However, many studies lack mechanistic meaning. Detailed understanding of the molecular mechanisms of ROS and appropriate measurement methods for both ROS and oxidative damage are necessary for progress.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Yue Wu, Zhongting Zhao, Naidi Yang, Chenqi Xin, Zheng Li, Jiajia Xu, Bo Ma, Kah-Leong Lim, Lin Li, Qiong Wu, Changmin Yu, Chengwu Zhang
Summary: Parkinson's disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic neurons in the midbrain. Oxidative stress is believed to be the main cause of PD, and antioxidants have shown potential for its treatment. The study investigated the effects of vitamin B12 (VB12) supplementation in cellular and animal models of PD, and found that VB12 reduced reactive oxygen species (ROS) generation, improved motor dysfunction, and protected against mitochondrial dysfunction and dopaminergic neuron loss. These findings suggest that vitamin supplementation may be a safer and more feasible approach for PD intervention compared to chemical drug treatment.
Article
Immunology
Nan-Nan Liang, Ying Zhao, Yue-Yue Guo, Zhi-Hui Zhang, Lan Gao, De-Xin Yu, De-Xiang Xu, Shen Xu
Summary: This study found that mitochondria-derived reactive oxygen species (ROS) contribute to renal cell ferroptosis during lipopolysaccharide (LPS)-induced acute kidney injury (AKI). Inhibition of ferroptosis and scavenging of mitochondrial ROS with specific inhibitors, ferrostatin-1 and MitoQ, respectively, attenuated renal lipid peroxidation, ferroptosis-characteristic mitochondrial damage, and renal cell death. These findings suggest that mitochondria-targeted antioxidants may be potential therapeutic agents for sepsis-induced AKI.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Peripheral Vascular Disease
Hewang Lee, Xiaoliang Jiang, Imran Perwaiz, Peiying Yu, Jin Wang, Ying Wang, Maik Huttemann, Robin A. Felder, David R. Sibley, Brian M. Polster, Selim Rozyyev, Ines Armando, Zhiwei Yang, Peng Qu, Pedro A. Jose
Summary: Overproduction of reactive oxygen species (ROS) is important in hypertension, while dopamine D-5 receptor (D5R) is known to decrease ROS production. Activation of autophagy by D5R plays a role in inhibiting mito-ROS production in the kidneys.
HYPERTENSION RESEARCH
(2021)
Review
Agriculture, Dairy & Animal Science
Ahmad Yar Qamar, Muhammad Ilyas Naveed, Sanan Raza, Xun Fang, Pantu Kumar Roy, Seonggyu Bang, Bereket Molla Tanga, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: Male fertility is negatively affected by endogenous stressors, such as reactive oxygen species (ROS), which reduce fertility. However, proper levels of ROS are necessary for sperm to complete various cellular functions. Excessive ROS production leads to oxidative stress (OS) and impairs sperm functions, resulting in male infertility. Numerous studies have shown that the use of natural and synthetic antioxidants can improve sperm quality-related parameters. This review focuses on the causes of OS, sources of ROS production, mechanisms of sperm damage, and the role of antioxidants in preserving sperm fertility.
Article
Genetics & Heredity
Islam M. M. Saadeldin, Bereket Molla Tanga, Seonggyu Bang, Abdulkadir Y. Y. Maigoro, Heejae Kang, Dabin Cha, Soojin Lee, Sanghoon Lee, Jongki Cho
Summary: This study identified 29 known mature miRNAs and 17 novel miRNAs in honeybee royal jelly extracellular vesicles (RJEVs). Further investigation showed that RJEVs can reduce apoptosis and enhance cell vitality and healing ability. The findings suggest that RJEVs regulate gene expression to maintain cell survival and promote cell regeneration.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Veterinary Sciences
Sanghoon Lee, Hyo-Gu Kang, Pil-Soo Jeong, Bong-Seok Song, Won Seok Choi, Yeung Bae Jin, Jae-Won Huh, Sun-Uk Kim, Bo-Woong Sim
Summary: This study provides the first evidence for the presence of melatonin synthesis enzyme, melatonin membrane receptors, and a melatonin binding site in non-human primate cumulus-oocyte complexes (COCs).
JOURNAL OF MEDICAL PRIMATOLOGY
(2023)
Article
Oncology
Xun Fang, Seonggyu Bang, Bereket Molla Tanga, Chaerim Seo, Dongjie Zhou, Gyeonghwan Seong, Islam M. Saadeldin, Sanghoon Lee, Xiang-Shun Cui, Jongki Cho
Summary: Assisted reproductive technology has improved animal reproduction efficiency. However, polyspermy is a limitation in porcine IVF. This study investigated the effects of porcine oviduct epithelial cells (OEC-EVs) on sperm-oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. The results showed that treatment with OEC-EVs significantly increased cleavage rate, number of embryos, and decreased polyspermy rate compared to the control group. Additionally, OEC-EVs improved cortical granule concentration and distribution, increased oocyte mitochondrial activity, and increased the IVF success rate.
MOLECULAR MEDICINE REPORTS
(2023)
Article
Multidisciplinary Sciences
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
Summary: Combining rolipram and cilostamide as cAMP modulators can greatly enhance the developmental competence of in vitro matured porcine oocytes. Pre-treatment for four hours was found to maintain optimal gap junction communication. The combined treatment group showed higher glutathione levels, lower reactive oxygen species levels, and a higher maturation rate compared to the control and single treatment groups. Two-phase in vitro maturation resulted in higher cleavage and blastocyst formation rates in parthenogenetic activation and somatic cell nuclear transfer embryos.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Islam M. Saadeldin, Bereket Molla Tanga, Seonggyu Bang, Chaerim Seo, Abdulkadir Y. Maigoro, Heejae Kang, Dabin Cha, Sung Ho Yun, Seung Il Kim, Sanghoon Lee, Jongki Cho
Summary: This study successfully isolated, characterized, and utilized egg yolk nanovesicles (VVs) as a supplement for porcine embryo culture. The results showed that VVs enhanced embryonic cell count, reduced apoptosis, and promoted embryo attachment and embryonic stem cell outgrowth. These effects may be attributed to the protein and miRNA cargo contents of VVs, providing a foundation for further research on the application of egg yolk nanovesicles in human embryo culture.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Ye Eun Joo, Pil-Soo Jeong, Sanghoon Lee, Se-Been Jeon, Min-Ah Gwon, Min Ju Kim, Hyo-Gu Kang, Bong-Seok Song, Sun-Uk Kim, Seong-Keun Cho, Bo-Woong Sim
Summary: This study found that anethole (AN) has a positive impact on porcine embryonic development. It improves blastocyst formation rate, cell number, and survival rate, and activates the SHH signaling pathway against oxidative stress. These results suggest that AN could be used for large-scale production of high-quality porcine embryos.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Pil-Soo Jeong, Hae-Jun Yang, Se-Been Jeon, Min-Ah Gwon, Min Ju Kim, Hyo-Gu Kang, Sanghoon Lee, Young-Ho Park, Bong-Seok Song, Sun-Uk Kim, Deog-Bon Koo, Bo-Woong Sim
Summary: This study examined the role of luteolin in the maturation of mammalian oocytes. The results showed that supplementation of luteolin during in vitro maturation improved the developmental competence of oocytes. Furthermore, luteolin reduced oxidative stress and mitochondria-mediated apoptosis in oocytes, leading to improved maturation.
Article
Agriculture, Dairy & Animal Science
Pil-Soo Jeong, Hyo-Gu Kang, Bong-Seok Song, Sun-Uk Kim, Bo-Woong Sim, Sanghoon Lee
Summary: The present study aimed to investigate the restorative effects of SHH protein supplementation on the in vitro maturation (IVM) of low-quality porcine cumulus-oocyte complexes (COCs). The results showed that supplementation with SHH protein can improve the developmental competence of low-quality porcine COCs, indicating the importance of an active SHH signaling pathway for the acquisition of developmental competence in porcine COCs.
Article
Cell & Tissue Engineering
Islam M. Saadeldin, Seonggyu Bang, Abdulkadir Y. Maigoro, Sung Ho Yun, Seung Kim, Sanghoon Lee, Jongki Cho
Summary: This study isolated and characterized nanovesicles from mature porcine oocytes, which were named as intra-ooplasmic vesicles (IOVs) for the first time. Proteomic analysis revealed that these IOVs contained proteins related to reprogramming, antioxidative defense, cytoskeleton, heat shock proteins, and metabolism. Supplementing cultured fibroblasts with IOVs resulted in changes in cell morphology and the expression of pluripotency and trophoblastic markers.
CELLULAR REPROGRAMMING
(2023)
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Agriculture, Dairy & Animal Science
Se-Been Jeon, Pil-Soo Jeong, Min Ju Kim, Hyo-Gu Kang, Bong-Seok Song, Sun-Uk Kim, Seong-Keun Cho, Bo-Woong Sim
Summary: The study revealed that Lut enhances porcine embryonic development by reducing oxidative stress levels, increasing antioxidant capacity through activation of the Nrf2/Keap1 signaling pathway, and improving the developmental competence of embryos.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jihyun Park, Wonyou Lee, Islam M. Saadeldin, Seonggyu Bang, Sanghoon Lee, Junkoo Yi, Jongki Cho
Summary: This study aimed to assess the effects of embryonic developmental stage, quality grade, and fresh or frozen/thawed conditions on the pregnancy rate and sex ratio of live offspring in Hanwoo cows. The results showed that embryo quality, developmental stage, and cryopreservation significantly affected the pregnancy rate, but there was no significant difference in pregnancy rates between fresh and frozen/thawed embryos.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)