Review
Biochemistry & Molecular Biology
Eleni-Myrto Trifylli, Anastasios G. Kriebardis, Evangelos Koustas, Nikolaos Papadopoulos, Sofia Vasileiadi, Sotirios P. Fortis, Vassilis L. Tzounakas, Alkmini T. Anastasiadi, Panagiotis Sarantis, Effie G. Papageorgiou, Ariadne Tsagarakis, Georgios Aloizos, Spilios Manolakopoulos, Melanie Deutsch
Summary: This review provides an overview of the emerging role of extracellular vesicles in cholangiocarcinogenesis and their potential as diagnostic and therapeutic tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Peiwen Fu, Jianguo Zhang, Haitao Li, Michael Mak, Wenrong Xu, Zhimin Tao
Summary: Extracellular vesicles (EVs) have shown significant promises as nano-/micro-size carriers in drug delivery and bioimaging, possessing unique physicochemical properties, biological features, and mechanical aspects. This review analyzes the pros and cons of EVs as a delivery platform, elaborates on rational design improvements, and compares pharmaceutical loading approaches, aiming to construct a clinically available and effective nano-/micro-carrier.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Review
Chemistry, Multidisciplinary
Jin Wang, Derek Chen, Emmanuel A. Ho
Summary: Exosomes, as potential drug delivery vehicles, have been studied extensively, but face multiple technical challenges, including isolation, characterization, and stability testing, as well as challenges related to drug cell-uptake, loading, release, and in vivo distribution.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Oncology
Rajeswari Raguraman, Dhaval Bhavsar, Dongin Kim, Xiaoyu Ren, Vassilios Sikavitsas, Anupama Munshi, Rajagopal Ramesh
Summary: Exosomes are small vesicles that can be used as drug delivery vehicles for cancer treatment due to their unique characteristics. Preclinical studies have shown that exosomes can effectively deliver chemotherapeutics, biologicals, and natural products with potent anticancer activity. However, challenges in large-scale production, storage, and stability have limited the number of exosome-based clinical trials.
Review
Biochemistry & Molecular Biology
Urszula Szwedowicz, Zofia Lapinska, Agnieszka Gajewska-Naryniecka, Anna Choromanska
Summary: Until 30 years ago, extracellular vesicles (EVs) were believed to be used for removing unnecessary compounds from cells. However, their enormous potential for diagnosing and treating diseases has now been recognized. EVs play a crucial role in intercellular communication by transferring bioactive molecules between cells. Compared to lab-created drug nanocarriers, EVs are more stable in physiological conditions and have lower immunogenicity and cytotoxicity. EVs can deliver cargo to specific cells due to their membrane proteins and lipids. New strategies have been developed to create tailor-made therapeutic EVs. However, there are significant challenges in applying EVs as drug-delivery agents in clinical settings.
Review
Biochemistry & Molecular Biology
Yu-Chen Jia, Yi-Xuan Ding, Wen-Tong Mei, Yu-Ting Wang, Zhi Zheng, Yuan-Xu Qu, Kuo Liang, Jia Li, Feng Cao, Fei Li
Summary: Comprehensive reviews and large population-based cohort studies have significantly reduced the incidence and mortality of pancreatitis. The study of extracellular vesicles (EVs), especially exosomes, in pancreatitis has shown potential for identifying cell-to-cell communication and influencing the disease's development and progression. Plasma exosomes of acute pancreatitis can reach the alveolar cavity and activate alveolar macrophages to cause acute lung injury, suggesting their use as biomarkers and therapeutic agents for this disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Jun-Yong Wu, Yong-Jiang Li, Xiong-Bin Hu, Si Huang, Da-Xiong Xiang
Summary: Storage temperature and duration significantly impact the stability of sEVs, leading to changes in size distribution, quantity, contents, cellular uptake, and biodistribution. It is suggested to choose the appropriate storage temperature based on the requirements of the study.
Review
Oncology
Akhil Srivastava, Shipra Rathore, Anupama Munshi, Rajagopal Ramesh
Summary: Exosomes, as a natural form of cell communication, have potential applications as drug delivery vehicles and in cancer treatment. However, challenges such as sourcing and scaling up production need to be addressed for broader use.
SEMINARS IN CANCER BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Anna M. M. Timofeeva, Anastasia P. P. Paramonik, Sergey S. S. Sedykh, Georgy A. A. Nevinsky
Summary: Exosomes, as nanovesicles secreted by various cell types, have shown great potential as a means of targeted drug delivery due to their natural origin, high biocompatibility, and ability for loading different types of therapeutic molecules. Milk is a unique source of exosomes that are resistant to harsh digestive conditions, making them ideal for oral delivery. This review discusses scalable protocols for isolating and purifying exosomes from human, cow, and horse milk, as well as methods for drug loading and surface modification for more efficient and specific delivery. Furthermore, the review explores visualization techniques and cellular localization of drug-loaded exosomes in tissues. In conclusion, milk exosomes represent a new generation of targeted delivery agents, with further challenges for research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sarah A. Walsh, Benjamin W. Hoyt, Cassie J. Rowe, Devaveena Dey, Thomas A. Davis
Summary: Severe polytraumatic injury can lead to immune dysfunction, but early biomarkers for immune dysfunction are currently lacking. Study of exosomal cargo may provide critical insights for intervention.
Review
Pharmacology & Pharmacy
Jafar Rezaie, Vahid Nejati, Monireh Mahmoodi, Mahdi Ahmadi
Summary: Extracellular vesicles (EVs), particularly MSCs-EVs, have shown great potential as a drug delivery system in nanomedicine. They can selectively deliver therapeutic agents to target cells and improve various diseases. However, there are still challenges that need to be addressed for the clinical translation of modified MSCs-EVs.
BIOCHEMICAL PHARMACOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Javaria Munir, Alice Ngu, Haichuan Wang, Denise M. O. Ramirez, Janos Zempleni
Summary: Small extracellular vesicles (sEVs, exosomes) in milk have scalable production, protect cargo, can be absorbed and cross barriers, do not affect organ function or elicit immune responses, can be loaded with therapeutic cargo, and have potential for drug delivery.
PHARMACEUTICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Isha Gaurav, Abhimanyu Thakur, Ashok Iyaswamy, Xuehan Wang, Xiaoyu Chen, Zhijun Yang
Summary: This review summarizes recent studies on the development of EV-based DDS and its advantages compared to conventional NDDS. It compares liposomes and exosomes in terms of their distinct benefits in DDS, analyzes considerations for better isolation and characterization of EVs for DDS, summarizes different methods for EV surface modification, discusses different origins of EVs and their role in developing DDS, includes major methods for encapsulating therapeutic cargos in EVs, and highlights key challenges and important open questions for further investigation in developing more effective EV-based DDS.
Article
Pharmacology & Pharmacy
Bhagyashree S. Joshi, Hector Garcia Romeu, Aldy Aliyandi, Marcel P. de Vries, Inge S. Zuhorn
Summary: Cell-derived extracellular vesicles are considered as promising candidates for in vivo drug delivery due to their ability to enter cells and deliver cargo. This study investigated the transmission mechanism of DNAJB6 in EVs using HEK293T cells.
Article
Multidisciplinary Sciences
Lixue Wang, Guosheng Wang, Wenjun Mao, Yundi Chen, Md. Mofizur Rahman, Chuandong Zhu, Peter M. Prisinzano, Bo Kong, Jing Wang, Luke P. Lee, Yuan Wan
Summary: Researchers have developed a bioinspired material called eFT-CNV, which can be used as a drug delivery vehicle. They have demonstrated that eFT-CNV can efficiently and selectively bind to target cells and achieve cytosolic drug delivery through membrane fusion. Compared to other similar drug delivery systems, eFT-CNV significantly improves the treatment efficacy of drugs targeting the cytosol. Researchers believe that this bioinspired eFT-CNV holds great promise as a tool in nanomedicine and precision medicine.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Lanlan Yu, Ruonan Wang, Tao Wen, Lei Liu, Tao Wang, Shuli Liu, Haiyan Xu, Chenxuan Wang
Summary: Rapid antigen detection tests are crucial for the early diagnosis of SARS-CoV-2, and the discovery of a high-affinity binder is essential for the development of such tests. In this study, a peptide binder R1 was identified through surface biopanning, which exhibited high affinity for the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. The R1-functionalized gold nanoparticles showed concentration-dependent response to RBD and selectivity over other proteins.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Wenbo Zhang, Mingwei Liu, Yang Wang, Xin Wang, Ruonan Wang, Shuyuan Li, Lanlan Yu, Feiyi Zhang, Chenxuan Wang
Summary: Transferring structural information from amino acid sequence to macroscale assembly is achieved by designing two synthetic peptides, QNL-His and QNL-Arg, with one amino acid substitution and using scanning tunneling microscopy (STM) to determine their folding structure and beta-sheet supramolecular organization. The structural variations in beta-strand length distribution between QNL-His and QNL-Arg lead to distinguishable outcomes in their beta-sheet assembled fibrils and phase transitions. The comparison of their structures and macroscopic properties reveals the role of assembly in amplifying the structural variations associated with a single-site mutation from a single-molecule scale to a macroscopic scale.
Article
Chemistry, Multidisciplinary
Lanlan Yu, Ruonan Wang, Shucong Li, Ufuoma I. Kara, Eric C. Boerner, Boyuan Chen, Feiyi Zhang, Zhongyi Jian, Shuyuan Li, Mingwei Liu, Yang Wang, Shuli Liu, Yanlian Yang, Chen Wang, Wenbo Zhang, Yuxing Yao, Xiaoguang Wang, Chenxuan Wang
Summary: Scanning tunneling microscopy (STM) is used to distinguish 18 coexisting conformational substates of the β-strand of hIAPP 8-37 and analyze the peptide-peptide interactions. This method also validates the existence of multiple conformations in other β-sheet peptide assemblies. The results provide insights into the conformational ensemble and interpeptide interactions in β-sheet peptide assembly.
ACS CENTRAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Wenbo Zhang, Ruonan Wang, Mingwei Liu, Shucong Li, Asher E. Vokoun, Weichen Deng, Robert L. Dupont, Feiyi Zhang, Shuyuan Li, Yang Wang, Zhenyu Liu, Yongfang Zheng, Shuli Liu, Yanlian Yang, Chen Wang, Lanlan Yu, Yuxing Yao, Xiaoguang Wang, Chenxuan Wang
Summary: This study utilizes scanning tunneling microscopy (STM) to analyze conformational substate ensembles of beta sheet peptides. It shows that STM can capture a thorough picture of the conformational substates and can complement conventional characterization techniques.
