Article
Biotechnology & Applied Microbiology
Min Wang, Yi Guo, Zexing Deng, Peng Xu
Summary: In this paper, a novel organic-inorganic composite hydrogel was designed, which exhibited excellent mechanical properties and promoted stem cell osteogenic differentiation, showing potential application in bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lysann M. Kroschwald, Felix Allerdt, Anne Bernhardt, Sandra Rother, Kai Zheng, Iram Maqsood, Norbert Halfter, Christiane Heinemann, Stephanie Moeller, Matthias Schnabelrauch, Michael C. Hacker, Stefan Rammelt, Aldo R. Boccaccini, Vera Hintze
Summary: The study demonstrates that aECM containing BGN can promote osteogenic differentiation of hMSC while delaying cellular growth, with a stimulating effect on ALP activity and calcium deposition. Some donors show a synergistic effect between sGAG and BGN, contributing to bone regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Orthopedics
Sheng-li Xia, Zi-yuan Ma, Bin Wang, Feng Gao, Sheng-yang Guo, Xu-han Chen
Summary: This study provides a gene expression profile of bone mesenchymal stem cells (BMSCs) in patients with type II diabetes mellitus (T2DM) and osteoporosis (OP). The downregulation of FOXQ1 was found to be responsible for the reduced osteogenic potency of T2DM-BMSCs. These findings are important for understanding the mechanism and treatment of diabetic osteoporosis.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Plant Sciences
Avinash Sanap, Kalpana Joshi, Tejas Shah, Girish Tillu, Ramesh Bhonde
Summary: The study showed that Piper longum L. fruit extract significantly increased proliferation of WJMSCs and enhanced Ca2+ accumulation and matrix mineralization, along with promoting alkaline phosphatase (ALP) activity and the expression of key gene mRNAs. This suggests that the fruit extract can enhance osteogenic differentiation of WJMSCs, potentially serving as an anti-osteoporotic agent.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Dongdong Fei, Yanmin Xia, Qiming Zhai, Yazheng Wang, Feng Zhou, Wanmin Zhao, Xiaoning He, Qintao Wang, Yan Jin, Bei Li
Summary: This study focuses on the clonal osteogenic heterogeneity of periodontal ligament stem cells (PDLSCs) and uncovers a mechanism where exosome-mediated PINK1/Parkin-dependent mitophagy regulates interclonal communication among SCCs with osteogenic heterogeneity, promoting osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Ziyi Feng, Xin Su, Ting Wang, Shu Guo
Summary: This study identified five novel biomarkers related to MSCs osteogenesis and verified their potential roles in immunoregulation and inflammation through in vitro experiments.
Article
Engineering, Biomedical
Man Zhang, Qian Sun, Yiling Liu, Zhiqin Chu, Leixiao Yu, Yong Hou, Heemin Kang, Qiang Wei, Weifeng Zhao, Joachim P. Spatz, Changsheng Zhao, Elisabetta A. Cavalcanti-Adam
Summary: Tunable mechanical properties of hydrogels play a key role in regulating stem cell differentiation. By modulating cellular spatial sensing of integrin-adhesive ligands on low stiffness hydrogels, the mechanosensing and osteogenic differentiation of MSCs can be promoted to levels comparable with those observed on stiffer hydrogels. This approach opens up new possibilities for designing biomaterials and tissue scaffolds for regenerative therapeutics.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Cell Biology
Jianyun Liu, Shan He, Baicheng Ma, Xingnuan Li, Yaqin Wang, Jianjun Xiong
Summary: This study used quantitative proteomic analysis to screen for protein expression profiles involved in early-stage osteogenesis and adipogenesis of MSCs. Two upregulated proteins, FBLN2 and NPR3, were identified to participate in the osteogenic differentiation process. The proteomics analysis also revealed functional alterations associated with FBLN2 and NPR3 deficiency.
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Biotechnology & Applied Microbiology
Keyu Kong, Yongyun Chang, Yi Hu, Hua Qiao, Chen Zhao, Kewei Rong, Pu Zhang, Jingwei Zhang, Zanjing Zhai, Huiwu Li
Summary: This study demonstrated that nanotopography on titanium surfaces promotes osteogenesis by increasing nuclear localization of Yap and activating the expression of Piezo1. The research also revealed the regulatory relationship between Yap and Piezo1 and confirmed Piezo1 as a downstream effector of Yap in nanotube-stimulated osteogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Paula Muresan, Phoebe McCrorie, Fiona Smith, Catherine Vasey, Vincenzo Taresco, David J. Scurr, Stefanie Kern, Stuart Smith, Pavel Gershkovich, Ruman Rahman, Maria Marlow
Summary: This study developed polymeric microneedle (MN) patches for the treatment of CNS tumors. The results demonstrated successful drug release and penetration, providing a potential solution for local brain drug delivery.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Albatool Sabeeh Al-Badry, Mohammed Hussain Al-Mayahy, David J. Scurr
Summary: Hydrogel microneedles offer a promising approach for delivering drug molecules across the skin in a sustained release manner. The use of hydrogel microneedles containing acyclovir could improve bioavailability and patient compliance while reducing gastrointestinal side effects. The optimal formulation of the hydrogel microneedle array was found to have suitable needle formation, mechanical strength, and drug release over a 24-hour period. The permeation of acyclovir from the hydrogel microneedle array was significantly increased compared to the control, and the array was able to deliver a high percentage of the loading dose over 24 hours.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Neurosciences
Franziska Linke, James E. C. Johnson, Stefanie Kern, Christopher D. Bennett, Anbarasu Lourdusamy, Daniel Lea, Steven C. Clifford, Catherine L. R. Merry, Snow Stolnik, Morgan R. Alexander, Andrew C. Peet, David J. Scurr, Rian L. Griffiths, Anna M. Grabowska, Ian D. Kerr, Beth Coyle
Summary: By comparing the most aggressive subgroup (Group 3) with the intermediate (SHH) subgroup, crucial differences in tumour heterogeneity were identified in medulloblastoma (MB) in children. Group 3 exhibited multiple subpopulations with enhanced oxidative phosphorylation and tricarboxylic acid (TCA) cycle activity, while SHH had unique matrix-producing subpopulations containing sulfur-containing species such as collagen organizer lumican. Patients with high fumarate levels and activated stress response pathways showed poorer survival. Inhibition of NRF2 increased sensitivity to vincristine in Group 3 MB.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Matthew McGrath, Karolina Zimkowska, Katelyn J. Genoud, Jack Maughan, Javier Gutierrez Gonzalez, Shane Browne, Fergal J. O'Brien
Summary: A biomimetic, bilayered antimicrobial collagen-based scaffold was developed to deal with the etiology of diabetic foot ulcers (DFUs). The scaffold exhibited high structural stability, successfully inhibited the growth and infiltration of Staphylococcus aureus, and supported the proliferation of epidermal cells and vascularization. These results suggest that the bilayered scaffold is a promising candidate for enhancing diabetic wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Leonardo Contreas, Andrew L. Hook, David A. Winkler, Grazziela Figueredo, Paul Williams, Charles A. Laughton, Morgan R. Alexander, Philip M. Williams
Summary: Bacterial infections are becoming a greater concern due to antimicrobial resistance. The rational design of materials resistant to biofilm formation is a crucial strategy to prevent infections related to medical devices. Machine learning has been used to identify strong associations between bacterial adhesion and the physicochemical properties of polyacrylate libraries. However, the interpretability of nonlinear models is limited, making it challenging to understand the molecular interactions between materials and bacteria.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biophysics
Heba Khateb, Andrew L. Hook, Stefanie Kern, Julie A. Watts, Sonali Singh, Darryl Jackson, Luisa Marinez-Pomares, Paul Williams, Morgan R. Alexander
Summary: The use of OrbiTrap analyzer with SIMS (3D OrbiSIMS) enables label-free mass spectrometry of Psl, accurately identifying secondary ion peaks specific to the Psl structure. By studying P. aeruginosa biofilm and purified Psl, 17 peaks associated with Psl fragments within the biofilm matrix were confidently identified. Additionally, the neutral loss sequences approach can also identify multiple oligosaccharide fragments without a biological reference sample.
Article
Chemistry, Medicinal
Songtao Xue, Zhiwei Li, Xiaotong Ze, Xiuyuan Wu, Chen He, Wen Shuai, Maria Marlow, Jian Chen, David Scurr, Zheying Zhu, Jinyi Xu, Shengtao Xu
Summary: Excessive melanin deposition can cause various skin disorders. We discovered a series of novel tyrosinase inhibitor hybrids that can inhibit tyrosinase activity and reduce melanin content in the skin. Compound 11c showed the most potent activity against tyrosinase, with nanomolar IC50 values, significant antioxidant activity, and low cytotoxicity. It also exhibited excellent permeability in vitro and reduced melanin content in a guinea pig model of UV-induced skin pigmentation. Compound 11c holds promise as a potential therapy for treating skin hyperpigmentation.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Claudio Intini, Lia Blokpoel Ferreras, Sarah Casey, James E. Dixon, John P. Gleeson, Fergal J. O'Brien
Summary: A novel miR-activated scaffold was developed to enhance mesenchymal stem/stromal cells (MSC) chondrogenesis and cartilage repair through the delivery of an inhibitor to miR-221. The miR-activated scaffold successfully transfected human MSCs with the miR-221 cargo, promoting an improved cell-mediated chondrogenic response. This innovative scaffold shows promise in improving chondrogenesis and enhancing cartilage defect repair.
ADVANCED THERAPEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Kavya Ganabady, Nicola Contessi Negrini, Jacob C. Scherba, Brandon M. Nitschke, Morgan R. Alexander, Kyle H. Vining, Melissa A. Grunlan, David J. Mooney, Adam D. Celiz
Summary: In this study, we employed a high-throughput screening strategy to rapidly identify a biocompatible bone adhesive with high bond strength. Through thiol-ene click chemistry, a library of polymers was synthesized and a copolymer (1-S2-FT01) composed of TATATO and PETMP was identified as the most suitable candidate. This copolymer exhibited favorable mechanical properties, supported hMSC adhesion, adhered to bone tissue with similar strength to commercially available bone glues, and facilitated osteogenic differentiation of hMSCs and attachment of polymer scaffolds to bone tissue, making it an ideal alternative bone adhesive for orthopedic surgery.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Hannah Donnelly, Mark R. Sprott, Anup Poudel, Paul Campsie, Peter Childs, Stuart Reid, Manuel Salmeron-Sanchez, Manus Biggs, Matthew J. Dalby
Summary: There is a clinical demand for viable bone grafts, and tissue engineering strategies using mesenchymal stromal cells (MSCs) to create potential bone-graft materials can help fulfill this need. However, the long culture periods and cost associated with MSC osteogenesis in a clinical setting necessitate the investigation of strategies for optimizing cell production. In this study, a piezoelectric copolymer with a fibronectin network coating is used to enhance MSC adhesion and present growth factors, and electrical stimulation is applied to investigate the MSC response.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Giuseppe A. Asaro, Matteo Solazzo, Meenakshi Suku, Dahnan Spurling, Katelyn Genoud, Javier Gutierrez Gonzalez, Fergal J. O' Brien, Valeria Nicolosi, Michael G. Monaghan
Summary: Electroconductive biohybrid platforms were created by blending collagen and 2D MXene, which showed high biocompatibility and enhanced cell proliferation and spreading. The platforms also limited bacterial attachment and proliferation. Culturing neonatal rat cardiomyocytes and human induced pluripotent stem cell-derived cardiomyocytes on the substrates demonstrated increased cell growth and cx43 expression when stimulated with an external electric field. This in vitro study convincingly shows the potential of the engineered conductive biohybrid platform for cardiac tissue regeneration.
NPJ 2D MATERIALS AND APPLICATIONS
(2023)
Article
Cell & Tissue Engineering
Juan Antonio Romero-Torrecilla, Jose Maria Lamo-Espinosa, Purificacion Ripalda-Cemborain, Tania Lopez-Martinez, Gloria Abizanda, Luis Riera-Alvarez, Sergio Ruiz de Galarreta-Moriones, Asier Lopez-Barberena, Naiara Rodriguez-Florez, Reyes Elizalde, Vineetha Jayawarna, Jose Valdes-Fernandez, Miguel Echanove-Gonzalez de Anleo, Peter Childs, Elena de Juan-Pardo, Manuel Salmeron-Sanchez, Felipe Prosper, Emma Muinos-Lopez, Froilan Granero-Molto
Summary: During bone regeneration, a mimetic periosteum made of a biocompatible 3D polycaprolactone (PCL) melt electro-written membrane can effectively deliver osteochondroprogenitor cells and growth factors to the injured bone. Coated with poly(ethyl acrylate), the PCL membrane can be functionalized with fibronectin and low doses of recombinant human BMP-2 (rhBMP-2), resulting in sustained osteoinduction in vitro. In vivo experiments showed that the functionalized mimetic periosteum could promote efficient healing and functional recovery in critical-size femoral defects in rats, and it could also deliver transplanted periosteum-derived mesenchymal cells to the bone defect for improved treatment outcomes.
NPJ REGENERATIVE MEDICINE
(2023)
Review
Engineering, Biomedical
Tara K. Mcguire, Martyna Stasiewicz, Ian Woods, Adrian G. Dervan, Fergal J. O'Brien
Summary: Spinal cord injury is a devastating traumatic injury that often results in permanent loss of function. Gene therapy using nonviral nanoparticle vectors has the potential to improve spinal cord regeneration by targeting multiple aspects of the injury and enhancing specificity. Despite challenges, nanoparticle-mediated gene delivery holds great promise for the future of spinal cord injury treatment.
ADVANCED NANOBIOMED RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Liam A. Crawford, Valentina Cuzzucoli Crucitti, Amy Stimpson, Chloe Morgan, Jonathan Blake, Ricky D. Wildman, Andrew L. Hook, Morgan R. Alexander, Derek J. Irvine, Simon V. Avery
Summary: This study demonstrates a novel approach to crop protection using polymer materials, which resulted in a significant reduction in infection rate and had no negative impact on wheat growth. These findings validate the potential of using non-eluting polymers for fungal control.
Article
Cell & Tissue Engineering
Elloise Z. Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Jonathan West, Ben D. MacArthur, Patrick S. Stumpf, Richard O. C. Oreffo
Summary: Researchers have developed a new method to isolate and enrich skeletal stem cells from human bone marrow, which have the potential to differentiate into bone, cartilage, and fat cells. This study provides valuable insights for further understanding and utilization of these stem cells.
JOURNAL OF TISSUE ENGINEERING
(2023)