Cas9 deactivation with photocleavable guide RNAs

The promise and challenge of therapeutic genome editing

(2020) Jennifer A. Doudna   NATURE

The kill-switch for CRISPR that could make gene-editing safer

(2020) Elie Dolgin   NATURE

Highly Parallel Profiling of Cas9 Variant Specificity

(2020) Jonathan L. Schmid-Burgk et al.   MOLECULAR CELL

The Histone Chaperone FACT Induces Cas9 Multi-turnover Behavior and Modifies Genome Manipulation in Human Cells

(2020) Alan S. Wang et al.   MOLECULAR CELL

Very fast CRISPR on demand

(2020) Yang Liu et al.   SCIENCE

CRISPRoff enables spatio-temporal control of CRISPR editing

(2020) Jared Carlson-Stevermer et al.   Nature Communications

Precision control of CRISPR-Cas9 using small molecules and light

(2019) Soumyashree Ashok Gangopadhyay et al.   BIOCHEMISTRY

A rocky road for the maturation of embryo-editing methods

(2019) Vivien Marx   NATURE METHODS

A Snapshot on the Cis Chromatin Response to DNA Double-Strand Breaks

(2019) Thomas Clouaire et al.   TRENDS IN GENETICS

A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9

(2019) Basudeb Maji et al.   CELL

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Rationally Designed Small Nucleic Acid-Based Inhibitors of CRISPR-Cas9

(2019) Christopher L. Barkau et al.   Nucleic Acid Therapeutics

Large dataset enables prediction of repair after CRISPR–Cas9 editing in primary T cells

(2019) Ryan T. Leenay et al.   NATURE BIOTECHNOLOGY

DNA double-strand break repair-pathway choice in somatic mammalian cells

(2019) Ralph Scully et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

(2019) Kale Kundert et al.   Nature Communications

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Stabilization of chromatin topology safeguards genome integrity

(2019) Fena Ochs et al.   NATURE

Advances in genome editing through control of DNA repair pathways

(2019) Charles D. Yeh et al.   NATURE CELL BIOLOGY

Protein Dynamics in Complex DNA Lesions

(2018) Radoslav Aleksandrov et al.   MOLECULAR CELL

Enhanced Bacterial Immunity and Mammalian Genome Editing via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks

(2018) Ryan Clarke et al.   MOLECULAR CELL

Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks

(2018) Eva K. Brinkman et al.   MOLECULAR CELL

Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

(2018) Hao Yin et al.   Nature Chemical Biology

CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway

(2018) Chris D. Richardson et al.   NATURE GENETICS

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

(2018) Digvijay Singh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CRISPR, cancer, and p53

(2018) Leslie K. Ferrarelli   Science Signaling

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

(2018) Christopher R. Cromwell et al.   Nature Communications

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis

(2018) Digvijay Singh et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CRISPR, cancer, and p53

(2018) Leslie K. Ferrarelli   Science Signaling

Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures

(2018) Thomas Clouaire et al.   MOLECULAR CELL

Reprogramming human T cell function and specificity with non-viral genome targeting

(2018) Theodore L. Roth et al.   NATURE

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

(2018) Christopher A. Vakulskas et al.   NATURE MEDICINE

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Target-Specific Precision of CRISPR-Mediated Genome Editing

(2018) Anob M. Chakrabarti et al.   MOLECULAR CELL

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Synthetic Oligonucleotides Inhibit CRISPR-Cpf1-Mediated Genome Editing

(2018) Bin Li et al.   Cell Reports

Effect of wavelength and beam width on penetration in light-tissue interaction using computational methods

(2017) Caerwyn Ash et al.   LASERS IN MEDICAL SCIENCE

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics

(2017) John C Rose et al.   NATURE METHODS

Anti-CRISPR: discovery, mechanism and function

(2017) April Pawluk et al.   NATURE REVIEWS MICROBIOLOGY

Non-homologous DNA end joining and alternative pathways to double-strand break repair

(2017) Howard H. Y. Chang et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

(2017) François Aymard et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors

(2017) Dirk A. Kleinjan et al.   Nature Communications

A damaged genome’s transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks

(2017) Fabio Iannelli et al.   Nature Communications

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

(2017) Holly A. Rees et al.   Nature Communications

Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

(2017) François Aymard et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence

(2017) Ting Zheng et al.   Scientific Reports

Disabling Cas9 by an anti-CRISPR DNA mimic

(2017) Jiyung Shin et al.   Science Advances

Naturally Occurring Off-Switches for CRISPR-Cas9

(2016) April Pawluk et al.   CELL

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR-Cas9 nuclear dynamics and target recognition in living cells

(2016) Hanhui Ma et al.   JOURNAL OF CELL BIOLOGY

Two-Stage Synapsis of DNA Ends during Non-homologous End Joining

(2016) Thomas G.W. Graham et al.   MOLECULAR CELL

DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks

(2016) Megan van Overbeek et al.   MOLECULAR CELL

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Distinct patterns of Cas9 mismatch tolerancein vitroandin vivo

(2016) Becky X.H. Fu et al.   NUCLEIC ACIDS RESEARCH

Proteomics insights into DNA damage response and translating this knowledge to clinical strategies

(2016) Louise von Stechow et al.   PROTEOMICS

Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

(2015) James E Dahlman et al.   NATURE BIOTECHNOLOGY

Cas9 gRNA engineering for genome editing, activation and repression

(2015) Samira Kiani et al.   NATURE METHODS

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Dynamics of CRISPR-Cas9 genome interrogation in living cells

(2015) Spencer C. Knight et al.   SCIENCE

Non-redundant Functions of ATM and DNA-PKcs in Response to DNA Double-Strand Breaks

(2015) Pierre Caron et al.   Cell Reports

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

The DNA damage response: The omics era and its impact

(2014) Kasper W.J. Derks et al.   DNA REPAIR

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Transcriptionally active chromatin recruits homologous recombination at DNA double-strand breaks

(2014) François Aymard et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position

(2013) Jason D Buenrostro et al.   NATURE METHODS

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The Sequence Alignment/Map format and SAMtools

(2009) H. Li et al.   BIOINFORMATICS

The MRN complex

(2008) Agnieszka Rupnik et al.   CURRENT BIOLOGY

53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility

(2008) Nadya Dimitrova et al.   NATURE