Article
Multidisciplinary Sciences
Pia Sommerkamp, Alexander C. Sommerkamp, Petra Zeisberger, Paula Leonie Eiben, Andreas Narr, Aylin Korkmaz, Adriana Przybylla, Markus Sohn, Franciscus van der Hoeven, Kai Schoenig, Andreas Trumpp
Summary: In this study, an inducible Pabpn1 mouse model was generated and validated, demonstrating the important role of Pabpn1 in hematopoiesis. This model provides a valuable tool for studying the function of PABPN1 and APA regulation in different tissues and disease settings.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Mengfei Liu, Linlin Hao, Sien Yang, Xiaohui Wu
Summary: Poly(A) tails play an essential role in regulating gene expression, and there is currently a lack of easy-to-use tools for measuring poly(A) tails in different sequencing protocols. In this study, we developed PolyAtailor, a unified and efficient framework for identifying and analyzing poly(A) tails, and compared its performance with other methods.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Cell Biology
Matti Turtola, M. Cemre Manav, Ananthanarayanan Kumar, Agnieszka Tudek, Seweryn Mroczek, Pawel S. Krawczyk, Andrzej Dziembowski, Manfred Schmid, Lori A. Passmore, Ana Casanal, Torben Heick Jensen
Summary: The study identified three mechanisms controlling polyadenylation in S. cerevisiae, with the primary regulator being PABP Nab2p, followed by PABP Pab1p and CPF. These mechanisms prevent uncontrolled polyadenylation, ensuring proper mRNA export and translation.
GENES & DEVELOPMENT
(2021)
Article
Biology
Rachael Emily Turner, Paul F. Harrison, Angavai Swaminathan, Calvin A. Kraupner-Taylor, Belinda J. Goldie, Michael See, Amanda L. Peterson, Ralf B. Schittenhelm, David R. Powell, Darren J. Creek, Bernhard Dichtl, Traude H. Beilharz
Summary: This study investigates the regulation of mRNA function by 3' end formation factors, revealing that different drugs can impact the selection of poly(A) addition sites by influencing nucleotide levels and chromatin landscape to regulate alternative polyadenylation.
Review
Cell Biology
Pawel Joachimiak, Adam Ciesiolka, Grzegorz Figura, Agnieszka Fiszer
Summary: Repeat expansion diseases are a group of disorders that affect the nervous and/or muscular system, with potential therapeutic targets in mutant mRNA. However, there is limited research on polyadenylation events for implicated transcripts.
Article
Microbiology
Yujiao Wu, Ying Yu, Quan Sun, Yixiang Yu, Jie Chen, Tian Li, Xianzhi Meng, Guoqing Pan, Zeyang Zhou
Summary: In this study, a gene called TRAPa (HNbTRAPa) was characterized in the microsporidia Nosema bombycis. TRAPa is a functional component of the ER translocon and is involved in substrate-specific protein translocation, a feature conserved in animals but absent in most fungi. The coding sequence of HNbTRAPa is longer than other homologs in microsporidia, and two mRNA isoforms resulting from non-canonical alternative polyadenylation were identified. The localization of HNbTRAPa in the microsporidia showed different patterns during the proliferation and spore stages. This study demonstrates the presence of post-transcriptional regulation and expands the repertoire of mRNA isoforms in microsporidia.
Review
Biochemistry & Molecular Biology
Vytaute Boreikaite, Lori A. Passmore
Summary: The formation of the 3' end of a eukaryotic mRNA is a crucial step in mRNA maturation. Protein factors such as CPSF and CPF are responsible for cleaving the pre-mRNA, adding a poly(A) tail, and regulating transcription. The selection of the cleavage site and the length of the poly(A) tail play important roles in mRNA translation, stability, and localization.
ANNUAL REVIEW OF BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Osamu Sano, Masahiro Ito, Masayo Saito, Akinori Toita, Toshio Tanaka, Hironobu Maezaki, Shinsuke Araki, Hidehisa Iwata
Summary: Phenotypic screening has identified a new modality of compounds called molecular glues, which induce the degradation of target proteins through ternary complexes of E3 ligases. In this study, a novel Cyclin K degrader, T4, was identified using global proteomic analysis, originally discovered through phenotypic screening for alternative poly-adenylation regulation. Further analysis revealed that T4 induces Cyclin K degradation and regulates alternative polyadenylation. Additionally, a more potent Cyclin K degrader, TR-213, was generated through a structure-activity relationship study of T4, providing novel chemical tools for studying Cyclin K degradation and alternative polyadenylation regulation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Yan Lu, Yijia Shao, Yinping Xie, Huinan Qu, Da Qi, Yuan Dong, Qiu Jin, Liping Wang, Junyuan Wei, Chengshi Quan
Summary: Overexpression of CLAUDIN6 can inhibit the malignant behavior of breast cancer by suppressing cell proliferation, migration, and invasion. CLAUDIN6 achieves this effect by inhibiting the ERK/Sp1/cyclin D1 and ERK/IL-8 signaling pathways.
CELLULAR SIGNALLING
(2022)
Article
Genetics & Heredity
Jianguo Feng, Xichao Xu, Xin Fan, Qian Yi, Liling Tang
Summary: The study reveals that the alternative splicing of Cyclin D1 is altered in cells under mechanical stress, with SRSF1 and BAF57/SMARCE1 potentially serving as critical regulators of this process.
Article
Oncology
Yan Liu, Ai Zhang, Ping-Ping Bao, Li Lin, Yina Wang, Haijian Wu, Xiao-Ou Shu, Aiguo Liu, Qiuyin Cai
Summary: The study reveals that miR-374b modulates the malignant behavior of breast cancer cells by negatively regulating CCND1 and TGFA genes, shedding light on the molecular mechanisms of breast cancer progression.
Article
Biotechnology & Applied Microbiology
Abdul K. Siraj, Sandeep Kumar Parvathareddy, Padmanaban Annaiyappanaidu, Saeeda O. Ahmed, Nabil Siraji, Asma Tulbah, Fouad Al-Dayel, Dahish Ajarim, Khawla S. Al-Kuraya
Summary: The study demonstrated that cyclin D1 over-expression in Middle Eastern breast cancers is associated with favorable prognostic features, serving as an independent predictor of overall survival and recurrence-free survival. Cyclin D1 expression was also found to be associated with phospho-Rb in luminal subtype of breast cancer, predicting luminal A breast cancer independently.
ONCOTARGETS AND THERAPY
(2021)
Article
Multidisciplinary Sciences
Agnieszka Tudek, Pawel S. Krawczyk, Seweryn Mroczek, Rafal Tomecki, Matti Turtola, Katarzyna Matylla-Kulinska, Torben Heick Jensen, Andrzej Dziembowski
Summary: RNA polyadenosine tails, crucial for mRNA stability and translation, vary in length depending on mutations in yeast exonuclease, deadenylase, and poly(A) polymerase. The study demonstrates the impact of poly(A) tails on mRNA and ncRNA functions, highlighting their responses to growth conditions.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Zhengyan Yang, Hongyun Xu, Yupo Yang, Chaoqun Duan, Pai Zhang, Yang Wang, Kai Fu, Yonghong Shen, Marvin Xuejun Xu
Summary: In this study, a new naphthalene derivative called SMY002 was synthesized, which showed potent inhibitory effects on the STAT3 signal in TNBC cells. SMY002 directly interacted with the SH2 domain of STAT3, leading to inhibition of phosphorylation, dimerization, nuclear distribution, transcriptional activity, and target genes expression of STAT3. SMY002 also suppressed migration, invasion, survival, growth, and metastasis of TNBC cells in vitro and in vivo by down-regulating the expression of Cyclin D1 and MMP9.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Review
Endocrinology & Metabolism
Sarah A. A. Jeffreys, Therese M. M. Becker, Sarah Khan, Patsy Soon, Hans Neubauer, Paul de Souza, Branka Powter
Summary: This review evaluates the prognostic and predictive value of CCND1 amplification in breast cancer. The results show that CCND1 amplification is significantly associated with positive estrogen receptor status, cyclin D1 overexpression, shorter recurrence-free survival (RFS), and overall survival (OS). It is also predictive of reduced RFS and OS in patients treated with endocrine therapy.
FRONTIERS IN ENDOCRINOLOGY
(2022)