A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity

Detection of CRISPR adaptation

(2020) Anna Shiriaeva et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Glycine Cleavage System and cAMP Receptor Protein Co-Regulate CRISPR/cas3 Expression to Resist Bacteriophage

(2020) Denghui Yang et al.   Viruses-Basel

Cas3 Protein—A Review of a Multi-Tasking Machine

(2020) Liu He et al.   Genes

StpA represses CRISPR-Cas immunity in H-NS deficient Escherichia coli

(2020) Damjan Mitić et al.   BIOCHIMIE

Molecular basis of ferredoxin:NADP(+) reductase interactions with FNR binding domains from TROL and Tic62 proteins

(2020) Tadija Kekic et al.   JOURNAL OF MOLECULAR STRUCTURE

CRISPR adaptation from a structural perspective

(2020) Giedrius Sasnauskas et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

(2019) Adam E. Dolan et al.   MOLECULAR CELL

Therapeutic developments for Duchenne muscular dystrophy

(2019) Ingrid E. C. Verhaart et al.   Nature Reviews Neurology

Adaptation processes that build CRISPR immunity: creative destruction, updated

(2019) Chun H. Lau et al.   Essays in Biochemistry

Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli

(2019) Susann M Fragel et al.   NUCLEIC ACIDS RESEARCH

Protein post-translational modifications in bacteria

(2019) Boris Macek et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas, DNA Supercoiling, and Nucleoid-Associated Proteins

(2019) Charles J. Dorman et al.   TRENDS IN MICROBIOLOGY

Harnessing type I CRISPR–Cas systems for genome engineering in human cells

(2019) Peter Cameron et al.   NATURE BIOTECHNOLOGY

The balancing act of R-loop biology: The good, the bad, and the ugly

(2019) Youssef A. Hegazy et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells

(2019) Hiroyuki Morisaka et al.   Nature Communications

BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra

(2018) András Micsonai et al.   NUCLEIC ACIDS RESEARCH

SWISS-MODEL: homology modelling of protein structures and complexes

(2018) Andrew Waterhouse et al.   NUCLEIC ACIDS RESEARCH

DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase

(2018) Tom Killelea et al.   RNA Biology

CRISPR-Cas guides the future of genetic engineering

(2018) Gavin J. Knott et al.   SCIENCE

Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex

(2018) Kaylee E. Dillard et al.   CELL

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

(2016) Kristina Majsec et al.   BMC MICROBIOLOGY

The discovery of CRISPR in archaea and bacteria

(2016) Francisco J. M. Mojica et al.   FEBS Journal

Cas3-Derived Target DNA Degradation Fragments Fuel Primed CRISPR Adaptation

(2016) Tim Künne et al.   MOLECULAR CELL

Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system

(2016) Raymond H. J. Staals et al.   Nature Communications

CRP represses the CRISPR/Cas system inEscherichia coli: evidence that endogenous CRISPR spacers impede phage P1 replication

(2014) Chi-Dung Yang et al.   MOLECULAR MICROBIOLOGY

Structures of CRISPR Cas3 offer mechanistic insights into Cascade-activated DNA unwinding and degradation

(2014) Yanwu Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3

(2014) Bei Gong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli

(2014) R. N. Jackson et al.   SCIENCE

Planting the seed: target recognition of short guide RNAs

(2014) Tim Künne et al.   TRENDS IN MICROBIOLOGY

Protospacer recognition motifs

(2013) Shiraz A. Shah et al.   RNA Biology

MOLE 2.0: advanced approach for analysis of biomacromolecular channels

(2013) David Sehnal et al.   Journal of Cheminformatics

CRISPR Immunity Relies on the Consecutive Binding and Degradation of Negatively Supercoiled Invader DNA by Cascade and Cas3

(2012) Edze R. Westra et al.   MOLECULAR CELL

H++ 3.0: automating pK prediction and the preparation of biomolecular structures for atomistic molecular modeling and simulations

(2012) R. Anandakrishnan et al.   NUCLEIC ACIDS RESEARCH

CRISPR Interference Directs Strand Specific Spacer Acquisition

(2012) Daan C. Swarts et al.   PLoS One

Helicase dissociation and annealing of RNA-DNA hybrids byEscherichia coliCas3 protein

(2011) Jamieson A. L. Howard et al.   BIOCHEMICAL JOURNAL

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

Structural and Biochemical Analysis of Nuclease Domain of Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Protein 3 (Cas3)

(2011) Sabin Mulepati et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structures of the RNA-guided surveillance complex from a bacterial immune system

(2011) Blake Wiedenheft et al.   NATURE

Structural basis for CRISPR RNA-guided DNA recognition by Cascade

(2011) Matthijs M Jore et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

High-temperature protein G is essential for activity of the Escherichia coli clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system

(2011) I. Yosef et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO

(2010) Edze R. Westra et al.   MOLECULAR MICROBIOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE