Journal
FOOD CHEMISTRY
Volume 339, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128090
Keywords
Lactoperoxidase; Human milk; Bovine milk; Goat milk; Camel milk; Amplex Red
Ask authors/readers for more resources
A more sensitive microplate fluorescent assay was developed for measuring LPO activity in milk, which is suitable for both laboratory and commercial scales. The method eliminates the influences of indigenous milk components and has good precision. The assay showed that LPO activities ranked as bovine > goat > camel > human in the four types of milk analyzed.
Lactoperoxidase (LPO) is one of the major antibacterial ingredients in milk and an extensively employed indicator for milk heat treatment. The traditional method for LPO activity measurement using ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulphonate) cannot achieve high sensitivity and is affected by indigenous milk thiocyanate. A more sensitive microplate fluorescent assay was developed by monitoring generation of red-fluorescent resorufin from LPO catalysed oxidation of Amplex (R) Red (1-(3,7-dihydroxyphenoxazin-10-yeethanone) in this study. The assay is particularly suitable for milk LPO activity measurement as it eliminates the influences of indigenous milk hydrogen peroxide and thiocyanate. The method limit of detection was 7.1x10(-6) U/mL of LPO in milk and good infra-run and inter-run precision was obtained. The LPO activities ranked as bovine > goat > camel > human in the four types of milk analysed. The high sensitivity and low cost of this assay makes it suitable for LPO activity analyses in both laboratory and commercial scales.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available