Article
Chemistry, Analytical
Mei-Hwa Lee, James L. Thomas, Zi-Lin Su, Wen-Kuan Yeh, Anna S. Monzel, Silvia Bolognin, Jens C. Schwamborn, Chien-Hsin Yang, Hung-Yin Lin
Summary: Molecularly imprinted polymer (MIP)-based electrochemical sensors were developed to detect the protein alpha-synuclein, which is a biomarker for Parkinson's disease. Doping the MIPs with TMDs enhances conductivity, resulting in sensors with a wide detection range and low detection limit. Analysis of cell culture medium from PD patient-specific midbrain organoids showed significantly reduced levels of alpha-synuclein, demonstrating the potential application of the sensor.
Article
Chemistry, Analytical
Mei-Hwa Lee, Cheng-Chih Lin, Piyush Sindhu Sharma, James L. Thomas, Chu-Yun Lin, Zofia Iskierko, Pawel Borowicz, Chien-Yu Lin, Wlodzimierz Kutner, Chien-Hsin Yang, Hung-Yin Lin
Summary: Instead of imprinting the entire protein molecule, imprinting protein epitopes is becoming more popular. MMP-1, belonging to the matrix metalloproteinase protein family, can serve as a disease protein biomarker. Epitopes of MMP-1 were identified and used to create a molecularly imprinted polymer. The polymer was optimized for conductivity and used to accurately determine MMP-1 concentration, which can be useful for early diagnosis.
Article
Chemistry, Analytical
Shiting Zhang, Zhiqiang Liu, Siyu Jin, Yufei Bai, Xingjia Feng, Guoqi Fu
Summary: Epitope imprinting on sacrificial asymmetric template-modified Janus nanocores is a promising approach for fabricating oriented surface epitope-imprinted open-mouthed polymer nanocapsules. The resulting OM-MIP NCs show fast fluorescence response and excellent target protein detection performance.
Article
Chemistry, Multidisciplinary
Zhanchen Guo, Rongrong Xing, Menghuan Zhao, Ying Li, Haifeng Lu, Zhen Liu
Summary: The study introduces a novel approach called ROSIC for engineering nanoparticles with specific targeting capability toward proteins and peptides. Using engineered coreless imprinted and cladded silica nanoparticles, the study demonstrates high specificity and high affinity for protein targeting. The approach successfully achieves targeted imaging of triple-negative breast cancer cells and mice, showing great promise for biomedical applications.
Article
Biophysics
Mei-Hwa Lee, Kai-Hsi Liu, James L. Thomas, Chen-Yuan Chen, Chuen-Yau Chen, Chien-Hsin Yang, Hung-Yin Lin
Summary: This study successfully synthesized molecularly imprinted polymers (MIPs) with specific epitope imprinting functionality by using novel peptides as templates and different concentrations of polymerization reactants. Doping MIPs with MXene significantly enhanced the sensing range and sensitivity of electrochemical response. The accuracy of this method was validated by measuring commercially available serum samples.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Tabkrich Khumsap, Suwussa Bamrungsap, Vu Thi Thu, Loc Thai Nguyen
Summary: A novel electrochemical sensor based on epitope-imprinted polydopamine was developed for sensitive and selective detection of ovalbumin. The sensor showed excellent sensitivity, a wide linear range, and good selectivity against other proteins, making it suitable for detecting allergic proteins in the food supply chain.
BIOELECTROCHEMISTRY
(2021)
Article
Biophysics
Shumeng Zhang, Jiwen Cui, Shi Zhou, Yanli Zhao, Jinlian Li, Dongmei Wu
Summary: In this study, independent functional modules for sensitive detection and signal separation of intracellular guanine (G) and xanthine (X) were constructed using molecular imprinting technology. These modules were integrated into a dual-functional module cellular electrochemical sensing platform, which showed good reproducibility and repeatability. The platform also exhibited a linear relationship between cell concentrations and the signals of G and X, and was able to track cell growth and viability. This study provides a foundation for the establishment of cell viability electrochemical detection method and offers new insights for simultaneous recording and tracking of multiple biomarkers.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Clinical Neurology
Brendan Monogue, Yixi Chen, Hadrian Sparks, Ranya Behbehani, Andrew Chai, Alexander J. Rajic, Aaron Massey, B. K. Kleinschmidt-Demasters, Matthieu Vermeren, Tilo Kunath, J. David Beckham
Summary: The normal function of alpha-synuclein in neurons is not clearly defined. This study found that alpha-synuclein functions in neurons to support immune signaling by co-localizing to the nucleus with interferon signaling proteins following interferon stimulation.
Article
Chemistry, Analytical
Jia-Yuan He, Hui-Xian Xu, Qin Li, Qin-Yue Zheng, Chong-Zhi Wang, Lian-Di Zhou, Ling Luo, Qi-Hui Zhang, Chun-Su Yuan
Summary: A novel MIP-based enzyme-free biosensor was developed for the selective detection of glycoprotein Trf. The sensor was prepared by electrochemical co-polymerization of hybrid monomers M-APBA and pyrrole on a GCE modified with cMWCNTs. The sensor exhibited great selective recognition ability and good analytical range with a low detection limit. The hybrid epitope and monomer-mediated imprinting strategy were successfully applied to detect Trf in human serum samples.
Article
Biochemistry & Molecular Biology
Michael Notaras, Aiman Lodhi, Friederike Dundar, Paul Collier, Nicole M. Sayles, Hagen Tilgner, David Greening, Dilek Colak
Summary: This study utilized patient-derived induced pluripotent stem cells to generate 3D cerebral organoids to model neuropathology of Schizophrenia. The results revealed specific alterations in neuronal programming factors, disrupted neurogenesis, and changes in growth factor expression, suggesting that Schizophrenia neuropathology could be encoded on a cell-type-by-cell-type basis. Single-cell sequencing identified the depletion of BRN2 and PTN, which were found to be mechanistic substrates of neurogenesis and cellular survival in Schizophrenia organoids.
MOLECULAR PSYCHIATRY
(2022)
Article
Chemistry, Analytical
Donglei Fu, Tao Chen, Yujun Cheng, Aihua Li, Honglei Liu, Zhongfa Cheng, Pengfei Li, Jingquan Liu
Summary: The bi-nanospherical signal amplification system constructed by molecular imprinting technique and gold nanoclusters shows excellent specificity in formaldehyde detection with a wide detection range and low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Oncology
Fernando Soto, Carlos F. Guimaraes, Rui L. Reis, Walfre Franco, Imran Rizvi, Utkan Demirci
Summary: This study reviews the development of three-dimensional artificial cell constructs and their application in modeling gastrointestinal dynamics. The fabrication of gastrointestinal organoids is achieved through self-assembly and engineering methods. The challenges and risks of translating gastrointestinal organoids from laboratory settings to patient-specific models in clinical applications are outlined.
Article
Cell Biology
Michael Fassler, Clara Benaim, Jacob George
Summary: The study found that the antibody sMB08 can protect neurons from the toxic effects of alpha Syn and promote its degradation, while reducing its uptake by microglia. When applied in experimental models, sMB08 can alleviate motor dysfunction in Parkinson's disease and preserve dopaminergic neurons.
Article
Biophysics
Shaopei Li, Kagan Kerman
Summary: Electrochemical biosensors have been utilized in studying biometal-protein interactions in neurodegenerative diseases such as Alzheimer's and Parkinson's. These sensors have shown promise in monitoring conformational changes induced by biometals and identifying disease biomarkers like amyloid-beta and alpha-synuclein.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Physical
Nabila Yasmeen, Mathieu Etienne, Piyush Sindhu Sharma, Wlodzimierz Kutner
Summary: This review summarizes the recent developments in the molecular imprinting approach for bacteria electrochemical sensing. Designing artificial molecularly imprinted polymer (MIP) and aptamer receptors for bacteria sensing is a challenging task due to the large size and complex cell walls of bacteria. Furthermore, the morphological characterization and recognition mechanism of MIPs are discussed in detail.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Cell Biology
Kathleen Mommaerts, Camille Bellora, Pauline Lambert, Seval Turkmen, Jens C. Schwamborn, Fay Betsou
Summary: The study aimed to optimize the protocol for skin biopsy dissociation, fibroblast culture, and cryopreservation for reprogramming into iPSCs in the context of biobank accreditation. Fibroblasts isolated using explant-based or enzymatic dissociation methods showed higher post-thaw viability, proliferative potential, and genome stability. The explant-based dissociation technique was found to have slightly higher reprogramming quality and successful recovery of iPSC clones compared to other methods.
BIOPRESERVATION AND BIOBANKING
(2022)
Article
Genetics & Heredity
Alise Zagare, Kyriaki Barmpa, Semra Smajic, Lisa M. Smits, Kamil Grzyb, Anne Grunewald, Alexander Skupin, Sarah L. Nickels, Jens C. Schwamborn
Summary: Human brain organoids are a valuable tool for studying neurodegenerative diseases like Parkinson's disease. This study shows that the in vitro model of human midbrain organoids accurately recapitulates the developmental path and cellular composition of the in vivo midbrain. The researchers also discovered that a specific genetic variant affects neurodevelopment and identified potential candidate genes associated with this variant.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Clinical Neurology
Semra Smajic, Cesar A. Prada-Medina, Zied Landoulsi, Jenny Ghelfi, Sylvie Delcambre, Carola Dietrich, Javier Jarazo, Jana Henck, Saranya Balachandran, Sinthuja Pachchek, Christopher M. Morris, Paul Antony, Bernd Timmermann, Sascha Sauer, Sandro L. Pereira, Jens C. Schwamborn, Patrick May, Anne Gruenewald, Malte Spielmann
Summary: In this study, the contribution of different cell types to Parkinson's disease pathology was investigated using single-nuclei RNA sequencing. The results revealed a disease-specific neuronal cell cluster and changes in glial cells, as well as the enrichment of Parkinson's disease risk genes. These findings highlight the importance of inflammatory signaling and immunomodulatory treatments in Parkinson's disease.
Article
Neurosciences
Veronica Mutti, Federica Bono, Zaira Tomasoni, Leonardo Bontempi, Adele Guglielmi, Silvia Bolognin, Jens C. Schwamborn, Cristina Missale, Chiara Fiorentini
Summary: The heteromer composed by dopamine D3 receptor and nicotinic acetylcholine receptor (D3R-nAChR heteromer) plays a crucial role in dopaminergic neurons, regulating structural plasticity and neuro-protection. Both nicotine and D3R agonist stimulation can induce remodeling effects through the D3R-nAChR heteromer. Activation of the PI3K-ERK1/2/Akt signaling pathways is associated with D3R-nAChR heteromer stimulation, while nicotine stimulation leads to persistent activation of Erk1/2 and Akt in a PI3K-independent way.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Clinical Neurology
Javier Jarazo, Kyriaki Barmpa, Jennifer Modamio, Claudia Saraiva, Sonia Sabate-Soler, Isabel Rosety, Anne Griesbeck, Florian Skwirblies, Gaia Zaffaroni, Lisa M. Smits, Jihui Su, Jonathan Arias-Fuenzalida, Jonas Walter, Gemma Gomez-Giro, Anna S. Monzel, Xiaobing Qing, Armelle Vitali, Gerald Cruciani, Ibrahim Boussaad, Francesco Brunelli, Christian Jager, Aleksandar Rakovic, Wen Li, Lin Yuan, Emanuel Berger, Giuseppe Arena, Silvia Bolognin, Ronny Schmidt, Christoph Schroeder, Paul M. A. Antony, Christine Klein, Rejko Kruger, Philip Seibler, Jens C. Schwamborn
Summary: This study investigated the differences between neurons derived from Parkinson's disease patients and controls, identifying potential pathways for targeted treatment. By correcting mutations and using a specific compound, improvements in metabolic properties and neuronal differentiation in patient-derived cells were observed. Treatment with a repurposed compound was shown to restore impaired dopaminergic differentiation in Parkinson's disease patient-derived cells.
MOVEMENT DISORDERS
(2022)
Article
Pharmacology & Pharmacy
Szilvia Veszelka, Maria Meszaros, Gergo Porkolab, Aniko Szecsko, Nora Kondor, Gyoergyi Ferenc, Tamas F. Polgar, Gabor Katona, Zoltan Kota, Lorand Kelemen, Tibor Pali, Judit P. Vigh, Fruzsina R. Walter, Silvia Bolognin, Jens C. Schwamborn, Jeng-Shiung Jan, Maria A. Deli
Summary: The study showed that targeting ligands on nanoparticles can enhance the uptake of drugs in rat brain endothelial cells, and modifying the negative zeta potential of endothelial cells or enzymatic treatment can significantly increase the uptake of cargo.
Article
Neurosciences
Sonia Sabate-Soler, Sarah Louise Nickels, Claudia Saraiva, Emanuel Berger, Ugne Dubonyte, Kyriaki Barmpa, Yan Jun Lan, Tsukasa Kouno, Javier Jarazo, Graham Robertson, Jafar Sharif, Haruhiko Koseki, Christian Thome, Jay W. Shin, Sally A. Cowley, Jens C. Schwamborn
Summary: The absence of microglia in current midbrain organoid systems limits the study of neuronal networks and neuroinflammation-related diseases. This study successfully derived microglia from human iPSCs and integrated them into midbrain organoids, shedding light on the role of microglia in influencing other cells in the organoids.
Article
Cell & Tissue Engineering
Pauline Mencke, Zoe Hanss, Javier Jarazo, Francois Massart, Arkadiusz Rybicki, Elizabet Petkovski, Enrico Glaab, Ibrahim Boussaad, Vincenzo Bonifati, Jens Christian Schwamborn, Wim Mandemakers, Rejko Krueger
Summary: In this study, an isogenic control cell line was generated using CRISPR/Cas9 technology to correct the disease-causing mutation in the PARK7 gene in a Parkinson's disease patient. This cell line will be used for phenotype analysis of neurons and astrocytes derived from the patient.
STEM CELL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Beatriz Garcia Santa Cruz, Jan Slter, Gemma Gomez-Giro, Claudia Saraiva, Sonia Sabate-Soler, Jennifer Modamio, Kyriaki Barmpa, Jens Christian Schwamborn, Frank Hertel, Javier Jarazo, Andreas Husch
Summary: The study focuses on addressing the challenges of data acquisition and image analysis in complex disease research. By combining traditional computer vision methods with deep learning, the research team successfully trained a deep learning network and improved the segmentation quality using automatically generated labels. The user-friendly graphical interface allows researchers to evaluate and correct the predictions. Furthermore, the study demonstrates the feasibility of training a deep learning solution on a large dataset of noisy labels.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Arianna Minoia, Luca Dalle Carbonare, Jens Christian Schwamborn, Silvia Bolognin, Maria Teresa Valenti
Summary: Degenerative diseases affecting bone tissues and the brain have significant socioeconomic impact. Osteoporosis and neurodegenerative diseases are often linked, with bone fractures and reduced mobility being common in patients. Bone is not only involved in movement but also plays a role in mineral metabolism and the production of stem cells. The interaction between the nervous system and bone is bidirectional, with bone influencing neuronal regulation and vice versa. This review summarizes recent knowledge on this interaction and emphasizes its importance. Experimental models and suggestions for evaluating molecular interactions between nerve and skeletal cells are also discussed.
Article
Cell Biology
Maria Meszaros, Thi Ha My Phan, Judit P. Vigh, Gergo Porkolab, Anna Kocsis, Emese K. Pali, Tamas F. Polgar, Fruzsina R. Walter, Silvia Bolognin, Jens C. Schwamborn, Jeng-Shiung Jan, Maria A. Deli, Szilvia Veszelka
Summary: Research has found that nanoparticles targeting cerebral endothelial cells can enhance drug delivery to the brain. By combining alanine and glutathione, these polypeptide nanocarriers showed increased permeability and cellular uptake in a co-culture model of human endothelial cells and brain pericytes. These findings suggest that poly(l-glutamic acid) nanoparticles can be effective nanocarriers for drug delivery to the nervous system.
Article
Cell & Tissue Engineering
Axel Chemla, Giuseppe Arena, Claudia Saraiva, Clara Berenguer-Escuder, Dajana Grossmann, Anne Grunewald, Christine Klein, Philip Seibler, Jens C. Schwamborn, Rejko Kruger
Summary: In this study, two Parkinson's disease patients' primary skin fibroblasts carrying distinct heterozygous mutations in the RHOT1 gene encoding Miro1 were reprogrammed into induced pluripotent stem cells (iPSCs) using episomal reprogramming. Isogenic gene-corrected lines were generated using CRISPR/Cas9 technology. Both isogenic pairs were comprehensively characterized and quality assured to study the Miro1-related molecular mechanisms underlying neurodegeneration in iPSC-derived neuronal models.
STEM CELL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Manuela Cassotta, Hugo Geerts, Lise Harbom, Tiago F. Outeiro, Iosif Pediaditakis, Orly Reiner, Stefan Schildknecht, Jens C. Schwamborn, Jarrod Bailey, Kathrin Herrmann, Helena T. Hogberg
Summary: Parkinson's disease (PD) is a complex neurodegenerative condition with a lack of preventive or curative therapies. New approach methodologies (NAMs) hold potential to advance PD research and reduce the reliance on animal-based studies. Key recommendations to advance PD research include integrating NAMs, learning from other neurodegenerative diseases, increasing data sharing, promoting innovative pilot studies, and accessing philanthropic funding.
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
(2022)
Review
Engineering, Biomedical
Sonia Sabate-Soler, Michela Bernini, Jens Christian Schwamborn
Summary: This review discusses the achievements in integrating microglia into brain organoids, including cell organization, ultrastructure, cell signaling, and their interactions and functionality with other cell types. It particularly focuses on the interaction between microglia and the midbrain and dopaminergic systems, as well as therapeutic approaches for neuroinflammation and disease modeling.
PROGRESS IN BIOMEDICAL ENGINEERING
(2022)
Article
Endocrinology & Metabolism
Egle Danileviciute, Ni Zeng, Christophe M. Capelle, Nicole Paczia, Mark A. Gillespie, Henry Kurniawan, Mohaned Benzarti, Myriam P. Merz, Djalil Coowar, Sabrina Fritah, Daniela Maria Vogt Weisenhorn, Gemma Gomez Giro, Melanie Grusdat, Alexandre Baron, Coralie Guerin, Davide G. Franchina, Cathy Leonard, Olivia Domingues, Sylvie Delhalle, Wolfgang Wurst, Jonathan D. Turner, Jens Christian Schwamborn, Johannes Meiser, Rejko Krueger, Jeff Ranish, Dirk Brenner, Carole L. Linster, Rudi Balling, Markus Ollert, Feng Q. Hefeng
Summary: In this study, the researchers found that the protein DJ-1 plays a crucial role in regulating PDH activity in T-reg cells, especially in aged mice. This discovery provides new insights into the regulation of the PDH complex and suggests the DJ-1-PDHB axis as a potential target for maintaining or re-establishing T-reg homeostasis.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
