Article
Chemistry, Analytical
Qitian Lin, Junye Wu, Lili Jiang, Dexian Kong, Chao Xing, Chunhua Lu
Summary: The study developed an electrochemical biosensor based on Mg2+-dependent DNAzyme for simultaneous detection of miRNA-21 and miRNA-141, showing high sensitivity and selectivity. The biosensor offers advantages such as simple preparation and enzyme-free signal amplification, making it suitable for various bioanalysis and clinical diagnostics.
Article
Chemistry, Analytical
Zhangcheng Fu, Tong Lv, Lili Jiang, Chao Xing, Chunhua Lu
Summary: The study developed a novel electrochemical biosensor for the analysis of tumor biomarker microRNA-141, with a wide linear range and low detection limit. The biosensor demonstrated high sensitivity and selectivity, making it a promising tool for sensitive detection of various biomarkers in clinical applications.
Review
Biochemistry & Molecular Biology
Ping Zhao, Dewei Jiang, Yunchao Huang, Ceshi Chen
Summary: EphA2, a receptor tyrosine kinase overexpressed in human breast cancers, plays important roles in malignant breast progression and has become a promising therapeutic target for breast cancer treatment.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Review
Medicine, General & Internal
Silvia Grassilli, Valeria Bertagnolo, Federica Brugnoli
Summary: miR-29b has a controversial role in breast cancer, acting both as a tumor suppressor and as an onco-miRNA. The different isoforms of miR-29b have a dual effect on breast tumor features, depending on the prevailing function, mature miR-29b expression, and breast tumor characteristics. miR-29b-3p and miR-29b1-5p play distinct roles in breast tumors with different phenotypes.
Review
Biochemistry & Molecular Biology
Xiyin Li, Lina Zhao, Ceshi Chen, Jianyun Nie, Baowei Jiao
Summary: This review summarizes the regulatory mechanisms of EGFR protein expression in breast cancer cells, including mutations, amplification, endocytic dysfunction, recycling acceleration, and degradation disorders. Potential therapeutic strategies targeting EGFR, such as reducing protein expression, precise clearance of target proteins, and inhibiting non-EGFR signaling pathways, are also discussed.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Chemistry, Analytical
Aiting Cai, Luxia Yang, Xiaoxia Kang, Jinxia Liu, Feng Wang, Haiwei Ji, Qi Wang, Mingmin Wu, Guo Li, Xiaobo Zhou, Yuling Qin, Li Wu
Summary: An electrochemical-DNA (E-DNA) sensor was developed using DNA metallization as a signal reporter and hybridization chain reaction (HCR) as a signal amplification strategy. The sensor demonstrated superior sensing performance for trace ctDNA analysis, with a wide detection range and low detection limit. It also showed excellent selectivity, repeatability, stability, and recovery, making it a promising tool for clinical sample analysis.
Article
Oncology
Aditi S. Khatpe, Rebecca Dirks, Poornima Bhat-Nakshatri, Henry Mang, Katie Batic, Sarah Swiezy, Jacob Olson, Xi Rao, Yue Wang, Hiromi Tanaka, Sheng Liu, Jun Wan, Duojiao Chen, Yunlong Liu, Fang Fang, Sandra Althouse, Emily Hulsey, Maggie M. Granatir, Rebekah Addison, Constance J. Temm, George Sandusky, Audrey Lee-Gosselin, Kenneth Nephew, Kathy D. Miller, Harikrishna Nakshatri
Summary: TONSL is upregulated during the initial steps of tumorigenesis to support neoplastic transformation by increasing DNA repair and represents a potential therapeutic target for treating breast cancer.
Article
Chemistry, Analytical
Jialin Zhang, Hongli Yang, Wenjing Liu, Herui Wen, Fengjiao He
Summary: Diseases caused by bacteria present significant challenges to human health. This study developed a rapid bacteria 16S rDNA electrochemical sensor using a target-triggered hairpin self-assembly and tripedal DNA walker amplification strategy. The sensor showed low-cost, scalable, and enhanced detection sensitivity by continuously recycling low-concentration targets. The sensor had a limit of detection of 20 CFU mL(-1) for detecting Staphylococcus aureus (S. aureus) and a detection time of less than 3 hours, making it potentially useful for clinical early diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Medicine, Research & Experimental
Liu Ye, Beibei Liu, Jingling Huang, Xiaolin Zhao, Yuan Wang, Yungen Xu, Shuping Wang
Summary: Doublecortin-like kinase 1 (DCLK1) is a significant prooncogenic factor that is strongly associated with the malignant progression and clinical prognosis of various cancers. DCLK1 plays important roles in stem cell marker regulation, tumor cell reprogramming, and immune evasion. However, the exact biological functions of DCLK1, especially the disparities between its alpha- and beta-form transcripts in cancer progression, remain ambiguous.
Review
Genetics & Heredity
Yunqing Zeng, Huimin Rong, Jianwei Xu, Ruyue Cao, Shuhua Li, Yanjing Gao, Baoquan Cheng, Tao Zhou
Summary: DNA methylation plays a significant role in the occurrence and development of gastric cancer, and research in this area is crucial for finding cancer biomarkers.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Analytical
Jiaxue Yu, Jingju Liu, Chong-Bo Ma, Lijuan Qi, Yan Du, Xintong Hu, Yanfang Jiang, Ming Zhou, Erkang Wang
Summary: This study proposes an electrochemical signal-on strategy for highly sensitive detection of HBV drug-resistant mutations, providing a new approach for developing cost-effective diagnostic methods. By utilizing LAMP isothermal amplification technique, the method shows potential for wide application in clinical HBV diagnosis and detection of other disease genes.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Yanming Ma, Minchao Liu, Mengmeng Hou, Yufang Kou, Wenxing Wang, Tiancong Zhao, Xiaomin Li
Summary: In this study, a surface curvature-induced oriented assembly strategy was used to synthesize a sushi-like novel Janus therapeutic nanoplatform, which consists of two functional units, FeOOH nanospindles as the CDT subunit and mSiO(2) nanorods as the drug-loading subunit. The FeOOH CDT subunit is half covered by the mSiO(2) nanorod, forming a sushi-like structure. The FeOOH nanospindle is about 400 nm in length and 50 nm in diameter, while the mSiO(2) nanorod is about 550 nm in length and 100 nm in diameter. The FeOOH subunit can be reduced by endogenous GSH and catalyze H2O2 in TME to achieve efficient CDT, while the mSiO(2) subunit can release doxorubicin for combined chemotherapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Wenxi Hu, Yuanyuan Chang, Junqing Huang, Yaqin Chai, Ruo Yuan
Summary: In this study, a tetrahedral DNA nanostructure with multiple recognition domains was used to construct a highly sensitive electrochemical biosensor for the quantitative detection of a tumor-associated protein. The new nanostructure improved capture efficiency and sensitivity compared to traditional nanostructures. This strategy provides a new approach for sensitive detection in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Pan Xie, Ding Wang, Hongwen Zhao, Na Yin, Shuang Hu, Wenhan Qin, Li Meng, Xin Pan, Yali Yuan, Ruo Yuan, Kanfu Peng
Summary: Fe-N-doped carbon nanomaterials were designed as a novel biomimetic enzyme with peroxidase-like activity, combined with glucose oxidase for enzyme cascade catalytic amplification, and further combined with target-induced DNA walker amplification to develop a sensitive electrochemical biosensor for thrombin detection. The biomimetic enzyme cascade reaction coupled the advantages of natural enzyme and nanozyme, providing a new approach for constructing artificial multienzyme amplification systems in biosensing, bioanalysis, and disease diagnosis applications.
Article
Chemistry, Analytical
Wan Huang, Yue Zhou, Danyan Zhan, Guosong Lai
Summary: A novel biosensing method for ultrasensitive electrochemical detection of kanamycin antibiotic was developed by inducing the assembled formation of DNA nanostructures at an electrode. Dual signal amplification assisted by Exo I and HRP led to a very low detection limit of 9.1 fg mL(-1) for Kana assay.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Meiwen Wang, Yingying Tang, Yaoyao Chen, Ya Cao, Guifang Chen
ANALYTICA CHIMICA ACTA
(2019)
Article
Chemistry, Analytical
Yifei Li, Hong Chen, Yuhao Dai, Tingjun Chen, Ya Cao, Juan Zhang
ANALYTICA CHIMICA ACTA
(2019)
Article
Biochemical Research Methods
Xingwei Zhai, Jingwen Li, Ya Cao, Xiaoli Zhu, Yuguo Tang, Guifang Chen, Kun Han
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2019)
Article
Biophysics
Ya Cao, Lingling Li, Bing Han, Ying Wang, Yuhao Dai, Jing Zhao
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Electrochemistry
Jingwen Li, Yifei Li, Xingwei Zhai, Ya Cao, Jing Zhao, Yuguo Tang, Kun Han
ELECTROCHEMISTRY COMMUNICATIONS
(2020)
Review
Chemistry, Analytical
Xiaomeng Yu, Lingjun Sha, Langjian Dong, Ya Cao, Jing Zhao
Summary: Exosomes play a crucial role in intercellular transport and communication, and have the potential to serve as biomarkers for early detection and real-time monitoring of tumors. This review provides a summary of the recent advances in tumor exosome detection and their potential applications.
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Bing Han, Cheng Fang, Lingjun Sha, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: A new fluorescent aptasensor using cascade strand displacement reaction was proposed for the detection of ochratoxin A, achieving an improved detection limit and short assay time. The method showed potential for use in food quality control in the future.
Article
Biophysics
Ya Cao, Ying Wang, Xiaomeng Yu, Xihui Jiang, Gang Li, Jing Zhao
BIOSENSORS & BIOELECTRONICS
(2020)
Review
Chemistry, Analytical
Yuhao Dai, Bing Han, Langjian Dong, Jing Zhao, Ya Cao
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Bing Han, Langjian Dong, Lingling Li, Lingjun Sha, Ya Cao, Jing Zhao
SENSORS AND ACTUATORS B-CHEMICAL
(2020)
Article
Chemistry, Analytical
Ying Wang, Langjian Dong, Jing Zhao, Mohammed Jalalah, M. S. Al-Assiri, Farid A. Harraz, Ya Cao
Summary: The developed dual-functional probe sensing method can specifically identify CD44 even in serum samples and breast cancer cells with broad detection range and high sensitivity. The method can monitor changes in CD44 expression, providing a new approach to study stem-like properties in breast cancer.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Yingyan Zhao, Qi Liu, Yujia Qin, Yue Cao, Jing Zhao, Kai Zhang, Ya Cao
Summary: In this work, an electrochemical aptasensor was designed for the high-accuracy assay of AFP-L3 ratio. The aptasensor demonstrated the ability to accurately measure the AFP-L3 ratio in a linear range of 0.0008-40 ng mL-1, with satisfactory specificity and good recoveries in complex serum samples. This aptasensor may serve as a valuable tool for the measurement of AFP-L3 ratio and have great potential for early warning of hepatocellular carcinoma in clinical application.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Xiaomeng Yu, Ya Cao, Yingyan Zhao, Jianan Xia, Jie Yang, Yuanyuan Xu, Jing Zhao
Summary: This study presents a universal method for accurate and sensitive analysis of tumor-associated glycoprotein biomarkers. The method utilizes dual recognition of carbohydrates and proteins to achieve amplified electrochemical responses for glycoprotein analysis. The results show high accuracy, specificity, and versatility in complex serum samples, making this method a valuable tool for the analysis of glycoprotein biomarkers.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xu Chen, Xianyong Wu, Ziyu He, Juan Zhang, Ya Cao, Dongsheng Mao, Chang Feng, Bo Tian, Guifang Chen
NEW JOURNAL OF CHEMISTRY
(2020)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)