Article
Chemistry, Multidisciplinary
Hai-Dong Xu, Xiaotong Cheng, Xianbao Sun, Peiyao Chen, Wenjun Zhan, Xiaoyang Liu, Xinliang Wang, Bing Hu, Gaolin Liang
Summary: A self-assembling probe for apoptosis imaging was designed, which underwent a Caspase-3 cleavage and CBT-Cys click reaction to form Gd nanoparticles. This probe achieved enhanced MR imaging of apoptosis in vitro, in apoptotic cells, and in zebrafish.
Article
Biophysics
Qiao-Yan Jiang, Ximin Cui, Yang Sun, Zhengsheng Mao, Jie Wang, Feng Chen, Jianfang Wang, Yue Cao
Summary: In this study, a new strategy for simultaneous detection of different substances on a single probe was developed. The fabricated AuNPL-crown nanoprobe allows for dual-channel and real-time tracking of the dynamic changes in caspase-3 and H2O2. This new platform provides high accuracy, sensitivity, convenience, and efficiency for the analysis of caspase-3 and H2O2 in the biological environment.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Engineering, Environmental
Qiangting Zheng, Qinyi Li, Sen Hu, Xiaoyu Guo, Haifeng Yang
Summary: An abnormal intake of Cu2+ can lead to cellular homeostasis disruption, oxidative damage, cardiovascular disorders, severe neurodegenerative disorders, and cancer. A rapid SERS assay for picomolar copper ions is developed based on the click reaction of Cu+-catalyzed alkyne azide cycloaddition. This method shows high sensitivity and selectivity and can be used for water quality assessment in onshore and offshore water treatment systems.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Xun Liu, Xingxiang Wang, Sujuan Ye, Ronghua Li, Hongxia Li
Summary: The study introduces a novel "one-two-three" system for enhanced imaging and detection of miRNA-21 in cancer cells, as well as chemogene therapy. The system features a miR-21-triggered endogenous substance-accelerated cyclic reaction, two signal switch modes, and three functions including enhanced imaging, detection, and comprehensive treatment.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Francesca Anson, Pintu Kanjilal, S. Thayumanavan, Jeanne A. Hardy
Summary: Cytosolic protein delivery shows promise in various applications. However, the use of fluorescent tags can lead to undesired consequences which are often overlooked, making distinguishing between cytosolically released and endosomally entrapped proteins challenging. The development of a caspase-3 variant tagged with GFP aims to address this issue and facilitate the study of therapeutic properties and apoptotic cell death mechanisms.
Article
Nanoscience & Nanotechnology
Hee-Kyung Na, Jisun Ki, Minh-Uyen Le, Kyoung-Shim Kim, Chul-Ho Lee, Tae Geol Lee, Jung-Sub Wi
Summary: The study developed a new type of SERS probe formed by enzyme reactions in the presence of Raman reporters, which achieved uniform and stable signal generation for biomolecule detection on chips over long periods. By applying this approach to a SERS-based immunohistochemistry assay, successful multiplexed protein detection was achieved in brain tissue from transgenic mice.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Spectroscopy
Xiaoyuan Ma, Jingna Zhang, Zhouping Wang
Summary: The study focused on real-time detection of caspase 3 in living cells using a dual-mode nanosensor based on SERS-fluorescence. The sensor showed good linear relationship between caspase 3 and both SERS and fluorescence signals, with a minimum detection limit of 0.001 nM. Activation of caspase 3 was observed in cells stimulated by AFB1, providing detailed information for apoptosis monitoring.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Jiajun Liu, Fangzheng Wu, Minghui Wang, Menglin Tao, Zhengjie Liu, Zijuan Hai
Summary: A tumor-targeted fluorescent/photoacoustic probe was developed to monitor the activity of Caspase-3 in vivo. The probe could enter and accumulate in tumor cells, resulting in increased fluorescence and photoacoustic signals. This study provides a new method for clinical imaging of tumor apoptosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Biology
Rui Gong, Dianbing Wang, Ghulam Abbas, Shimin Li, Qian Liu, Mengmeng Cui, Xian-En Zhang
Summary: This study introduces a genetically encoded switch-on mNeonGreen2-based biosensor for detecting caspase-3 activity. The biosensor demonstrated shortened response time, higher sensitivity, and prolonged functional shelf life in detecting caspase-3 compared to existing counterparts. Additionally, the biosensor was used to evaluate the effect of various drugs on inducing apoptosis in HeLa and MCF-7 tumor cells and inhibiting Zika virus invasion.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Qing Pei, Shaojin Lu, Junli Zhou, Bowen Jiang, Chaonan Li, Zhigang Xie, Xiabin Jing
Summary: The study developed an activatable prodrug and fluorescence imaging material by engineering the endogenous NAD(P)-H:quinone oxidoreductase-1 (NQO1) responsive linker, enabling tumor-specific chemotherapy and imaging in living mice. The enzyme-sensitive prodrug nanoparticles showed potent anticancer performance to NQO1-positive cancer with minimal off-target toxicity, providing an alternative strategy for constructing smart prodrug nanoplatforms for advanced cancer imaging and therapy.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Leyi Fang, Xiao-Tong Pan, Kang Liu, Dechen Jiang, Deju Ye, Li-Na Ji, Kang Wang, Xing-Hua Xia
Summary: We have developed a super-long silver nanowire-based surface-enhanced Raman scattering (SERS) detection strategy for simultaneous sensing of intracellular pH (pHi) and extracellular pH (pHe). This method is efficient, minimally invasive, and provides high sensitivity and spatial resolution.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Min Zhang, Zijun Li, Mengyang Zhang, Hang Heng, Shiying Xu, Zhaoyin Wang, Zhihui Dai
Summary: In this study, a sensing substrate for in situ Raman imaging analysis of biothiols in cells was constructed using DTNB modified on the surface of gold nanorods. The Raman signals of DTNB were greatly amplified by gold nanorods, and the variation in Raman signals allowed for sensitive quantification of three main biothiols. The DTNB-modified gold nanorods exhibited superior analytical performance compared to a commercial probe.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tzu-Han Lo, Hung-Lin Chen, Cheng- Yao, I-Chun Weng, Chi-Shan Li, Chi-Chun Huang, Nien-Jung Chen, Chun-Hung Lin, Fu-Tong Liu
Summary: The study demonstrates that galectin-3 enhances caspase-4/11 activation and oligomerization through binding with LPS, resulting in intensified pyroptosis. This finding provides potential opportunities for new therapeutic interventions in host resistance against bacterial infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Shabnam Mansuri, Tarushyam Mukherjee, Sriram Kanvah
Summary: This review focuses on recent literature published between 2020 and 2022, with particular emphasis on the use of extrinsic fluorophores to study sterol-driven biological processes, including sterol transport, biomolecular interactions, and biological imaging.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Yulong Jin, Kun Xu, Yanyan Huang, Huifei Zhong, Rui Zhao
Summary: The newly developed cell-permeable fluorescent probe can selectively exhibit a ratiometric response towards the apoptotic key enzyme caspase-3, offering potential for real-time quantification of caspase-3 activity to estimate apoptotic stages in living cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Zhe Yang, Wenshan He, Hongyi Zheng, Jielin Wei, Pei Liu, Wei Zhu, Leping Lin, Li Zhang, Changfeng Yi, Zushun Xu, Jinghua Ren
Article
Materials Science, Biomaterials
Wei Zhu, Yao Liu, Zhe Yang, Li Zhang, Liji Xiao, Pei Liu, Jing Wang, Changfeng Yi, Zushun Xu, Jinghua Ren
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Article
Materials Science, Biomaterials
Pei Liu, Hongyi Zheng, Zhe Yang, Li Ba, Wei Zhu, Leping Lin, Yuxuan Xiong, Zushun Xu, Jinghua Ren
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Article
Engineering, Environmental
Wei Zhu, Li Zhang, Zhe Yang, Pei Liu, Jing Wang, Jinguo Cao, Aiguo Shen, Zushun Xu
CHEMICAL ENGINEERING JOURNAL
(2019)
Article
Engineering, Biomedical
Pei Liu, Jinghua Ren, Yuxuan Xiong, Zhe Yang, Wei Zhu, Qianyuan He, Zushun Xu, Wenshan He, Jing Wang
Article
Chemistry, Multidisciplinary
Wei Zhu, Er-Li Cai, Hao-Zheng Li, Ping Wang, Ai-Guo Shen, Juergen Popp, Ji-Ming Hu
Summary: By designing small molecular monomers containing large Raman scattering cross-sections and polymerizing them at the nanoscale, smaller but brighter optical nanotags have been developed for high-throughput multi-color biomedical imaging, offering a new method for generating high sensitivity SRS imaging tags.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Jun Cao, Wei Zhu, Ai-Guo Shen, Ji-Ming Hu
Summary: The finely-designed nanocomposites CuS@Au@ZnPBA exhibit excellent photothermal properties and biosafety, effectively killing bacteria and accelerating wound healing, making them a promising antibacterial alternative.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Ya-Qin Liu, Wei Zhu, Quan Yuan, Ji-Ming Hu, Xin Zhang, Ai-Guo Shen
Summary: A direct photoreduction method was proposed for the rapid detection and killing of bacterial pathogens. The method synthesized SERS tags by integrating poly(4-cyanostyrene) nanoparticles and silver ions, and used 4-mercaptophenylboronic acid as a bridge and recognition molecule. The strategy achieved quick and sensitive bacteria counting and killing with high accuracy.
Article
Chemistry, Analytical
Wei Zhu, Jing-Yi Tang, Dong Yu, Ai-Guo Shen
Summary: Biofilms pose a great challenge for their anti-bacterial activity by obstructing drug action. Developing highly effective antibacterial agents to destroy biofilms is crucial. A new type of nanocomposite, poly(4-cyanostyrene)@silver@polylysine, is proposed, exhibiting excellent antibacterial efficacy through charge effect and Ag+ release. Nearly 100% antibacterial rates against S. aureus and E. coli were achieved, and the relationship between antibacterial efficiency and biofilm breakage was illustrated. These nanocomposites have great potential in antibacterial applications.
Article
Chemistry, Analytical
Jun Cao, Wei Zhu, Ji Zhou, Bai-Chuan Zhao, Yao-Yu Pan, Yong Ye, Ai-Guo Shen
Summary: The development of a convenient, sensitive, rapid, and self-sterilizing biosensor for microbial detection is of great importance for preventing and controlling foodborne diseases. In this study, a surface-enhanced Raman scattering (SERS) sensing nanoplatform was designed based on a capture-enrichment-enhancement strategy to detect bacteria. The SERS nanotags of gold-Azo@silver-cetyltrimethylammonium bromide (Au-Azo@Ag-CTAB) were obtained by optimizing the synthesis process conditions. The modified CTAB enabled the nanotags to electrostatically bind to different bacteria, allowing for fast (15 min), accurate, and sensitive detection (limit of detection: 300 and 400 CFU/mL for E. coli and S. aureus, respectively). Additionally, the endogenous antibacterial activity of CTAB ensured complete bacteria inactivation after the assay process, effectively avoiding secondary contamination.
Article
Nanoscience & Nanotechnology
Wei Zhu, Ya-Qin Liu, Pei Liu, Jun Cao, Ai-Guo Shen, Paul K. Chu
Summary: A smart hydrogel dressing capable of consuming blood glucose and generating antibacterial agents is designed to promote wound healing. The hydrogel degrades glucose to produce hydrogen peroxide and gluconic acid, supporting a chemodynamic reaction that kills bacteria. Additionally, it can convert near infrared light into heat for photothermal therapy. This multifunctional hydrogel has great potential in the treatment of diabetic wounds.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Dong Yu, Yao Shen, Wei Zhu, Ji-Ming Hu, Ai-Guo Shen
Summary: This study proposes a print-driven triple-bond coding mode using polymeric nanoparticles with Raman shifts for developing security inks. These inks can be used for printing invisible patterns and monochromatic patterns, as well as colorful graphics with different proportions. It is predicted that this technology will enhance anti-counterfeiting capabilities in the field.
Article
Chemistry, Multidisciplinary
Ya-Qin Liu, Wei Zhu, Ji-Ming Hu, Ai-Guo Shen
Summary: Prussian blue-based SERS tags have attracted increasing interest in bio-related analysis due to their zero-background, stable and strong Raman signal output, as well as outstanding multiplexing potential. They show promising applications in quantification analysis, multiple-spectral analysis, and cell-imaging joint phototherapy.
NANOSCALE ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Wei Zhu, Meng-Yue Gao, Qing Zhu, Bin Chi, Ling-Wen Zeng, Ji-Ming Hu, Ai-Guo Shen