Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 9, Pages 4326-4333Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.1c00089
Keywords
Antifouling; Electrochemical biosensor; Bovine serum albumin; Polyaniline nanowires; IgG
Categories
Funding
- National Natural Science Foundation of China [21974075, 21675093]
- Science and Technology Benefiting the People Project of Qingdao [20-3-4-53-nsh]
- Taishan Scholar Program of Shandong Province of China [ts20110829]
- Shandong Provincial Natural Science Foundation [ZR2019MB039]
- Shandong Key Laboratory of Biochemical Analysis [SKLBA1905]
- Doctoral Found of Taishan University [Y-01-2018018]
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An antifouling biosensing interface was constructed using cross-linked BSA and polyaniline nanowires, which exhibited enhanced antifouling capability and electrochemical activity. By immobilizing peptide aptamer for IgG recognition, an electrochemical biosensor with excellent selectivity and sensitivity was prepared, capable of accurately detecting IgG in complex biological samples.
Biofouling represents a serious challenge for the assaying of disease markers with various biosensors in complex biological samples due to the accompanied nonspecific protein adsorption. Herein, a highly sensitive and antifouling biosensing interface was constructed based on a cost-effective inert protein bovine serum albumin (BSA) cross-linked with polyaniline nanowires (PANI-NWs). Compared with the physically adsorbed BSA that was commonly used to block nonspecific adsorption or binding of proteins, the cross-linked BSA exhibited a significantly enhanced antifouling capability. The BSA/PANI-NW-modified electrode interface possessed excellent antifouling capability and electrochemical activity owing to the presence of the cross-linked BSA and the conducting polymer polyaniline. With further immobilization of the peptide aptamer for immunoglobulin G (IgG) recognition onto the BSA/PANI-NW interface, an electrochemical biosensor with excellent selectivity and sensitivity was prepared. The IgG biosensor possessed a linear range from 1.0 ng mL(-1) to 10 mu g mL(-1) and a low detection limit of 0.27 ng mL(-1), and it was capable of assaying IgG in complex human serum samples with acceptable accuracy when compared with the assay results obtained using commercial enzyme-linked immunosorbent assay kits. It is expected that the unique BSA-cross-linked conducting polymers can be used for the construction of various electrochemical sensors and biosensors that can be applied in complex biological media.
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