Journal
ACS OMEGA
Volume 6, Issue 1, Pages 656-665Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.0c05171
Keywords
-
Categories
Funding
- programs of Jilin Province Development and Reform Commission [2019C045-4]
- Science and Technology Department of Jilin Province [20190304102YY]
Ask authors/readers for more resources
This study found that polysaccharides from Bletilla striata (BSP) exhibit pro-coagulant and immunomodulatory activities, making them a potential coagulant. BSP-2, in particular, showed significant enhancement in immunomodulatory activity.
Bletilla striata is widely used for stanching bleeding. In this study, polysaccharides from B. striata (BSP) were extracted by hot water. Four polysaccharides named BSP-1-BSP-4 were fractionated using DEAE-52 cellulose. BSP fractions contained sulfate, and the degrees of substitution of BSP-3 and BSP-4 were 1.59 and 1.70, respectively. Analysis of monosaccharide composition showed that four polysaccharides were mainly composed of mannan and glucose. The in vitro results showed that BSP-1-BSP-4 elicited pro-coagulant capacities by shortening the activating partial thromboplastin time, prothrombin time, and thrombin time and elevating the fibrinogen content. Immunomodulatory activity was evaluated by MTT assay, the pinocytic capacity and NO production. Although BSP fractions did not affect RAW 264.7 cell viability, they, especially BSP-2, enhanced the immunomodulatory activity by increasing the pinocytic capacity and NO production. Overall, BSP may be developed as a potential coagulant with immunomodulatory effects.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available