Article
Virology
Anna Skelton, Leanne Frew, Richard Ward, Rachel Hodgson, Stephen Forde, Sam Mcdonough, Gemma Webster, Kiera Chisnall, Mary Mynett, Adam Buxton-Kirk, Aimee R. Fowkes, Rebecca Weekes, Adrian Fox
Summary: This study investigated the survival of Tomato brown rugose fruit virus on different greenhouse surfaces, the efficacy of different disinfectants, and the effects of heat treatment on the virus. The results showed that the virus can survive for extended periods on various surfaces and that hand washing is not reliable for virus removal. Glutaraldehyde and quaternary ammonium compound disinfectants were effective on all surfaces, while sodium hypochlorite had partial effectiveness against the virus.
Article
Plant Sciences
Nida' M. Salem, Motasem Abumuslem, Massimo Turina, Nezar Samarah, Abdullah Sulaiman, Barakat Abu-Irmaileh, Yousra Ata
Summary: This study investigated the natural occurrence and transmission of Tomato brown rugose fruit virus (ToBRFV) in Jordan. New weed host species were identified, and it was suggested that management practices focusing on these weed plants could effectively prevent the transmission of ToBRFV.
Article
Multidisciplinary Sciences
Angela Sloan, Todd Cutts, Bryan D. Griffin, Samantha Kasloff, Zachary Schiffman, Mable Chan, Jonathan Audet, Anders Leung, Darwyn Kobasa, Derek R. Stein, David Safronetz, Guillaume Poliquin
Summary: The effectiveness of sunlight in deactivating SARS-CoV-2 varies depending on the underlying matrix, with longer deactivation times observed in mucus than in tissue culture medium. This information is crucial for the development of infection prevention and control protocols to combat the spread of the deadly pathogen.
Article
Agronomy
N. Samarah, A. Sulaiman, N. M. Salem, M. Turina
Summary: Tomato brown rugose fruit virus (ToBRFV) is a highly virulent emerging virus species causing disease outbreaks and significant crop losses worldwide. Treatment with 2% hydrochloric acid or hydropriming can effectively eliminate ToBRFV from tomato seeds, leading to improved seed germination and seedling growth.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Yosuke Matsushita, Sawana Takeyama, Yasuhiro Tomitaka, Momoko Matsuyama, Kazuhiro Ishibashi, Hibiki Shinosaka, Kohei Osaki, Kenji Kubota
Summary: The seed-borne transmission of tomato brown rugose fruit virus (ToBRFV) was investigated in different cultivars of tomato, bell pepper, and eggplant. The virus was found to be restricted to the outer integument of the seed and transported through the vascular bundles during seed development. Tomato seeds transmitted the virus at rates reflective of the ineffectiveness of the resistance gene Tm-22 against ToBRFV. Bell pepper seeds had higher transmission rates compared to tomato seeds, but a resistant cultivar did not harbor the virus. Eggplant cultivars were systemically infected with ToBRFV but did not show symptoms, and their seeds did not transmit the virus.
JOURNAL OF GENERAL PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Avner Zinger, Moshe Lapidot, Arye Harel, Adi Doron-Faigenboim, Dana Gelbart, Ilan Levin
Summary: This study identified tomato genotypes resistant or tolerant to ToBRFV, characterized their virological and genetic traits, and developed DNA markers to expedite resistance breeding.
Article
Plant Sciences
N. M. Salem, A. Sulaiman, N. Samarah, M. Turina, M. Vallino
Summary: Tomato brown rugose fruit virus (ToBRFV) is a highly virulent emerging virus that can be transmitted through seeds, causing significant crop losses. The virus is mainly located on the external coat of tomato seeds, and the seed transmission rate is very low.
Article
Agriculture, Multidisciplinary
Hakan Fidan, Pelin Sarikaya, Kubra Yildiz, Bengi Topkaya, Gozde Erkis, Ozer Calis
Summary: The study aims to characterize symptoms caused by Tomato brown rugose fruit virus (ToBRFV) and establish a reliable detection method. Observations on infected tomato and pepper plants revealed specific symptoms, and PCR was successfully used for virus detection.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Horticulture
Aviv Dombrovsky, Netta Mor, Shelly Gantz, Oded Lachman, Elisheva Smith
Summary: Tomato brown rugose fruit virus (ToBRFV) and cucumber green mottle mosaic virus (CGMMV) have caused severe crop damages worldwide. Soil-mediated dispersion of tobamoviruses is a major hindrance towards mitigating disease spread. We have established a rigorous platform for studying disinfectant efficacy in greenhouses and found that chlorine-based disinfectants are significantly efficient in preventing disease spread of ToBRFV and CGMMV.
Article
Gastroenterology & Hepatology
Monique T. Barakat, Swarnadip Ghosh, Subhas Banerjee
Summary: The study assessed various approaches to minimize infection transmission costs and found that partially disposable duodenoscopes were the most favorable option from a cost utility standpoint. Fully disposable duodenoscopes minimized the potential for infection transmission and were more cost-effective than reprocessable duodenoscopes after single or double high-level disinfection, ethylene oxide sterilization, and culture and hold.
GASTROINTESTINAL ENDOSCOPY
(2022)
Article
Anesthesiology
T. Andrew Bowdle, Srdjan Jelacic, L. Silvia Munoz-Price, Marty Cohen, Sai M. Krishna, Lisa Brosseau
Summary: Respiratory viruses are mainly transmitted through respiratory particles, and disposable filtering facepiece respirators have been crucial during the COVID-19 pandemic, but reusable elastomeric respirators also have potential. However, existing elastomeric respirators need further development to improve their suitability.
Article
Plant Sciences
Michael Kravchik, Yulia Shnaider, Bekele Abebie, Meital Shtarkman, Reenu Kumari, Surender Kumar, Diana Leibman, Ziv Spiegelman, Amit Gal-On
Summary: The study found that SlTOM1a and SlTOM3 are essential for the replication of ToBRFV, but not for ToMV and TMV. SlTOM1b does not contribute to the resistance against ToBRFV and ToMV. SlTOM1a and SlTOM3 also regulate the tobamovirus susceptibility gene SlARL8a3 and plant development.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Or Eldan, Arie Ofir, Neta Luria, Chen Klap, Oded Lachman, Elena Bakelman, Eduard Belausov, Elisheva Smith, Aviv Dombrovsky
Summary: The tomato brown rugose fruit virus (ToBRFV) can infect both tomato and pepper plants, causing a hypersensitive response in both cases. However, the response is transient in pepper plants and not associated with fruit symptoms. Pepper plants without the L gene can also be infected by the virus without showing symptoms on fruits, but they can still serve as a source of transmission to adjacent tomato plants.
Review
Environmental Sciences
Ananda Tiwari, Nati Phan, Sarmila Tandukar, Razieh Ashoori, Ocean Thakali, Milad Mousazadesh, Mohammad Hadi Dehghani, Samendra P. Sherchan
Summary: This review paper summarizes the current knowledge surrounding possible fecal-oral transmission of SARS-CoV-2, emphasizing the active replication of the virus in the human gastrointestinal system and its presence in water. It also highlights the limitations of current detection methods in distinguishing between infectious and non-infectious viral particles, calling for further research and development of more accurate detection methods.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Ahmed Abdelfattah, Ayco J. M. Tack, Carolina Lobato, Birgit Wassermann, Gabriele Berg
Summary: This study proposes dividing the microbial inheritance process into three stages: plant to seed, seed dormancy, and seed to seedling. It discusses the factors affecting microbiome assembly during these stages, highlights future research directions, and emphasizes the implications of microbial inheritance for fundamental science and society.
TRENDS IN MICROBIOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Andrea Giovanni Caruso, Sofia Bertacca, Giuseppe Parrella, Roberto Rizzo, Salvatore Davino, Stefano Panno
Summary: This article reviews the emerging viral threat to tomato production caused by tomato brown rugose fruit virus (ToBRFV), a new highly infectious tobamovirus. The article discusses the origin, taxonomy, and genome of tomato, as well as the main diseases that undermine tomato production, focusing on viral pathogens. It also highlights the characteristics, detection methods, and disease management of ToBRFV, and suggests breeding as a new weapon to control its diffusion.
ANNALS OF APPLIED BIOLOGY
(2022)
Article
Agronomy
Marwa Mourou, Arafat Hanani, Anna Maria D'Onghia, Salvatore Walter Davino, Giorgio Mariano Balestra, Franco Valentini
Summary: This study identified potential antagonistic bacteria as biocontrol agents against X. fastidiosa, showing inhibitory activities against the pathogen. Further investigation is promising for developing an eco-sustainable strategy to control X. fastidiosa using biocontrol agents or their secreted metabolites.
Article
Plant Sciences
Sofia Bertacca, Andrea Giovanni Caruso, Daniela Trippa, Annalisa Marchese, Antonio Giovino, Slavica Matic, Emanuela Noris, Maria Isabel Font San Ambrosio, Ana Alfaro, Stefano Panno, Salvatore Davino
Summary: A real-time loop-mediated isothermal amplification (LAMP) assay was developed for the simple and efficient detection of Olea europaea geminivirus (OEGV) in olive cultivation areas. The assay showed high sensitivity and specificity and could be used for field diagnosis. A simplified sample preparation procedure was established to monitor OEGV spread. The survey revealed a relatively high incidence of OEGV.
Article
Agronomy
Andrea Giovanni Caruso, Sofia Bertacca, Arianna Ragona, Slavica Matic, Salvatore Davino, Stefano Panno
Summary: This study investigated the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in Sicilian grapevine cultivars, detecting their presence and spread. The results showed a positive rate of 5.34% for GLRaV-1 and 22.36% for GLRaV-3, with variations in different cultivars.
Article
Agronomy
Valentina Candian, Chiara D'Errico, Roberto Pierro, Stefano Panno, Salvatore Davino, Emanuela Noris, Rosemarie Tedeschi, Slavica Matic
Summary: A simple and rapid in-field real-time LAMP protocol was developed to detect the presence of Flavescence Doree phytoplasma (FDp) in the insect vector Scaphoideus titanus. This method is simple, sensitive, specific, cost-effective, and user-friendly, allowing for the direct detection of FDp on S. titanus in less than half an hour in the field.
Article
Agronomy
Andrea Giovanni Caruso, Sofia Bertacca, Arianna Ragona, Slavica Matic, Salvatore Davino, Stefano Panno
Summary: This study investigated the genetic structure and molecular variability of GVA in commercial vineyards in Sicily, Italy, finding a high infection rate of GVA in 11 autochthonous grapevine cultivars, with 'Nerello Mascalese' showing the highest incidence of infection.
Article
Plant Sciences
Andrea Giovanni Caruso, Arianna Ragona, Sofia Bertacca, Mauricio Alejandro Marin Montoya, Stefano Panno, Salvatore Davino
Summary: The study developed a real-time loop-mediated isothermal amplification (LAMP) assay for in-field detection of Tomato leaf curl New Delhi virus (ToLCNDV). The assay showed high sensitivity, specificity, and accuracy compared to conventional PCR. It also had a rapid sample preparation method, providing results in less than 45 minutes.
Article
Horticulture
Andrea Giovanni Caruso, Sofia Bertacca, Arianna Ragona, Graziella Agro, Maria Isabel Font-San-Ambrosio, Ana Alfaro-Fernandez, Rocio Estevez Sanchez, Stefano Panno, Salvatore Davino
Summary: This study evaluated the incidence of olive leaf yellowing-associated virus (OLYaV) in different regions of Italy and Spain using a real-time reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay. The assay showed high specificity and accuracy, with a higher sensitivity for OLYaV detection compared to conventional end-point RT-PCR. The survey revealed an infection rate of 46.25% in Spain and 30% in Italy, with certain olive cultivars being more susceptible to OLYaV. Additionally, the real-time RT-LAMP assay showed good sensitivity for detecting OLYaV in asymptomatic olive trees, making it a reliable and efficient method for routine testing of olive samples.