Article
Chemistry, Analytical
Ching-Hua Lee, Sung-Chun Tang, Ching-Hua Kuo
Summary: The study developed a sensitive and specific LC-MS/MS-MRM method to accurately identify PC(O-) and PC(P-), and applied it to investigate the regulation of etherPCs in primary neuronal cultures under hypoxic stress. Results revealed that the regulation of etherPCs was related to sn-1 chain length, while concentration changes of diacyl-PCs depended heavily on the degree of unsaturation.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Ariel A. C. Toledo Hijo, Aureliano A. D. Meirelles, Guilherme J. Maximo, Rosiane L. Cunha, Marcelo Cristianini, Thiago S. . Leite, Jorge F. B. Pereira, Antonio J. A. Meirelles
Summary: This article presents a strategy to obtain highly stable emulsions using ionic liquid (IL) technology, with ILs derived from fatty acids and choline as an example. These ILs exhibited high emulsifier ability and antimicrobial effectiveness at low concentrations, leading to improved shelf life of emulsions.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Food Science & Technology
Yue Wu, Yifan Chen, Min Zhang, Hitoshi Chiba, Shu-Ping Hui
Summary: This study investigated the plasmalogen content and composition in different porcine brain products using liquid chromatography-tandem mass spectrometry. The results showed that acetone precipitation, ethanol extraction, and drying did not significantly affect the plasmalogen content, while freeze-thaw cycles led to noticeable loss. The study also found that raw products and glycerophospholipid products had different plasmalogen profiles, and the concentration step seemed to impact the plasmalogen composition. The nutritional assessment revealed that porcine brain products had favorable values of multiple health-related indexes.
Article
Food Science & Technology
Wenhao Wang, Shohei Yamaguchi, Ayako Suzuki, Naomi Wagu, Masahiro Koyama, Akihiko Takahashi, Risa Takada, Koji Miyatake, Kozo Nakamura
Summary: The study showed that eggplants contain varying levels of ACh in different parts, with the fruit having the highest ACh content and the root having the lowest. ACh content increases with growth after flowering. Heat treatment does not affect ACh content in eggplants.
Article
Pharmacology & Pharmacy
Katharina Rox, Silke Rath, Dietmar H. Pieper, Marius Vital, Mark Broenstrup
Summary: A fast and cost-effective method based on liquid chromatography-tandem mass spectrometry that does not require isotope-labeled internal standards was reported for accurate quantification of TMAO and its precursors in real human plasma samples. By using an artificial plasma matrix for calibration, the analytical problem posed by the presence of endogenous TMAO and its precursors was circumvented, facilitating the establishment of TMAO as a routine clinical biomarker.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Review
Infectious Diseases
Fritz Michael Treiber, Heide Beranek-Knauer
Summary: The excessive use of antibiotics in animals can result in antibiotic residues in food, which may have harmful effects on human health and the environment. Monitoring and controlling the presence of antibiotic residues in animal products is crucial for food safety and public health.
Article
Food Science & Technology
Julio Cesar Espana Amortegui, Heidi Pekar, Mark Dennis Chico Retrato, Malin Persson, Bengt Karlson, Jonas Bergquist, Aida Zuberovic-Muratovic
Summary: A LC-MS/MS method was developed for the simultaneous identification and quantification of cyanotoxins in edible bivalves. The method successfully detected and resolved two previously detected cyanotoxin congeners separately. The method showed limitations for certain cyanotoxins but overall was specific and robust for the parameters investigated. The method was applied to analyze mussel and oyster samples collected along the Swedish coast, and the results can contribute to future regulatory monitoring for cyanotoxins to ensure seafood safety.
Article
Chemistry, Applied
Jiangbing Qiu, Lei Zhang, Yadong Cao, Peng Zhao, Jingrui Zhang, Aifeng Li
Summary: This study assessed the bioaccessibility of PSTs in cooked shellfish using an in vitro human digestion model. The results showed that different cooking methods reduced the content of PSTs in shellfish, but did not significantly impact their bioaccessibility. Transformation or degradation of PSTs occurred during the simulated digestion process.
Article
Chemistry, Applied
Maher Mahmoudi, Raoudha Abdellaoui, Faycal Boughalleb, Boutheina Yahia, Mahmoud Mabrouk, Nizar Nasri
Summary: This study investigated the lipid, protein, and bioactive compound contents in the seeds of Astragalus armatus, A. caprinus, and A. gombiformis, finding that they could be valuable assets for functional foods and/or food ingredients.
Article
Food Science & Technology
Hyo-Min Heo, Hyeong-Wook Jo, Hee-Ra Chang, Joon-Kwan Moon
Summary: A simultaneous analytical method using LC/MSMS and QuEChERS was developed for monitoring imidazolinone herbicides in livestock products. The method demonstrated simple, rapid, high throughput screening and quantitative analysis of imidazolinone herbicide residues in livestock products.
Article
Food Science & Technology
Randy L. Self, Michael G. McLendon, Christopher M. Lock, Jinxin Hu
Summary: This study collected 23 samples of seafood products internationally, evaluating their quality through sensory analysis and LC-HRMS. A model with a high accuracy of 99.6% was established, indicating consistency with sensory findings and reproducibility of the model. New compounds indicative of decomposition in seafood products were identified, enriching the knowledge in this field.
FOOD SCIENCE & NUTRITION
(2021)
Article
Agriculture, Multidisciplinary
Estelle Dreano, Charlotte Valentin, Jean-Francois Taillandier, Angelique Travel, Christophe Soumet, Arnaud Bridier, Dominique Hurtaud-Pessel, Michel Laurentie, Alexis Viel, Sophie Mompelat
Summary: The presence of veterinary drugs, including antibiotics, in feed produced from poultry byproducts such as feathers (processed to feather meal) may result in the persistence of antibiotics in feathers, plasma, and droppings of broiler chickens. A study on broiler chickens found that feathers contained a longer presence of antibiotics compared to plasma, with sulfadiazine being the most prevalent. The study also highlighted the potential transfer of resistant bacteria from droppings to feathers in floor-housed animals.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Seon Wook Kim, Da Jung Lim, In Seon Kim
Summary: A single-sample preparation method was developed for the simultaneous determination of fenpropimorph and fenpropimorph acid in livestock products, with good linearity, LOD and LOQ values. The method complied with CODEX guidelines and was successfully applied to real samples from domestic markets.
Article
Plant Sciences
Arghya Chattopadhyay, Anand Prakash Singh, Deepak Kasote, Indrajit Sen, Ahmed Regina
Summary: The study found that 30 mg kg(-1) of P was the optimal application amount to significantly reduce arsenic accumulation in rice grains, decrease arsenic toxicity on agronomic parameters and chlorophyll biosynthesis, and increase polyphenol levels in rice grains.
Article
Environmental Sciences
Marc Marin-Garcia, Celine F. Fabregas, Carla Argente, Jordi Diaz-Ferrero, Cristian G. Gomez-Canela
Summary: This study optimized an analytical method to determine the levels of PFAS in fish and seafood. The results showed that sardines, anchovies, and soles had the highest levels of PFAS, while cephalopods' spleens and crustaceans' heads had higher concentrations. The study also estimated the total intake of PFAS through fish and shellfish consumption for the Spanish adult population.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemical Research Methods
Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Takayuki Furukawa, Siddabasave Gowda B. Gowda, Yue Wu, Kazuhiro Nouso, Yuki Fujii, Yuki Yoshikawa, Hitoshi Chiba, Shu-Ping Hui
Summary: A highly sensitive LC-MS/MS method was developed to quantitatively analyze serum LysoPE species. The concentrations of LysoPEs were significantly decreased in NAFLD patients, with no significant difference between the SS and NASH groups.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Yasuyuki Tamai, Zhen Chen, Yue Wu, Jun Okabe, Yoshinao Kobayashi, Hitoshi Chiba, Shu-Ping Hui, Akiko Eguchi, Motoh Iwasa, Masaaki Ito, Yoshiyuki Takei
Summary: The combined treatment of BCAA and L-carnitine significantly improved hepatocellular function, reduced lipid deposition and adipophilin expression, and protected the liver by maintaining mitochondrial integrity in cirrhotic rats, supporting their therapeutic potential in human chronic liver diseases.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Reproductive Biology
Eri Furukawa, Zhen Chen, Hiroki Ueshiba, Yue Wu, Hitoshi Chiba, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano, Shu-Ping Hui
Summary: High circulating FFA concentrations postpartum may impact the quality of oocytes in postpartum cows, contributing to low fertility.
Article
Nutrition & Dietetics
Yusuke Yamamoto, Toshihiro Sakurai, Zhen Chen, Nao Inoue, Hitoshi Chiba, Shu-Ping Hui
Summary: The effects of lysophosphatidylethanolamine (lysoPE) on lipogenesis and lipolysis were investigated in this study, and the underlying mechanism was analyzed using LC-MS/MS and real-time PCR. The results revealed that lysoPE 18:2 is involved in lipid droplet formation by suppressing lipolysis and fatty acid biosynthesis, potentially playing a pathological role in the induction of fatty liver disease.
Article
Chemistry, Analytical
Lin Qao, Zhen Chen, Chunji Takada, Hitoshi Chiba, Ken-ichi Inoue, Shu-Ping Hui, Shen Ye
Summary: In this study, we investigated the degradation process of a major component of pulmonary surfactant (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), exposed to low-level ambient ozone. We found that low-level ozone can readily degrade POPC molecules and induce oxidation damage to unsaturated lipids, triggering lung inflammation.
ANALYTICAL CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Xunzhi Wu, Zhen Chen, Yue Wu, Yifan Chen, Jiaping Jia, Nianqiu Shen, Hitoshi Chiba, Shu-Ping Hui
Summary: In this study, the effect of flazin on lipid regulation was investigated at both the cell level and organelle level. The results showed that flazin significantly decreased cellular triglyceride and neutral lipid content, reduced lipid droplet size, and improved lipid droplet morphology. These findings suggest that flazin may be a potential therapeutic approach for treating lipid metabolism disturbances and contribute to the development of other nutraceuticals for related metabolic diseases.
Article
Chemistry, Applied
Zhen Chen, Jiaping Jia, Yue Wu, Hitoshi Chiba, Shu-Ping Hui
Summary: This study investigated the loss of plasmalogens in ready-to-eat tuna meat during storage under different conditions. It was found that repeated freeze-thaw cycles accelerated the loss of plasmalogens, and storage led to the accumulation of oxidized plasmalogen molecules. The loss of plasmalogens was strongly correlated with lipid oxidation and phospholipid degradation.
Article
Biochemistry & Molecular Biology
Zhen Chen, Rojeet Shrestha, Xiaoyue Yang, Xunzhi Wu, Jiaping Jia, Hitoshi Chiba, Shu-Ping Hui
Summary: This study investigated the chemical composition of lipid droplets (LDs) in human kidney cells under the influence of free fatty acids and oxidized lipoproteins. The results showed that both the fatty acids and oxidized lipoproteins significantly altered the lipid content and composition of LDs. The accumulation of oxidized lipids and dysregulation of lipid metabolism in LDs may be potential targets for the diagnosis and treatment of chronic kidney disease (CKD).
Article
Reproductive Biology
Eri Furukawa, Zhen Chen, Tomoaki Kubo, Yue Wu, Koichiro Ueda, Madalitso Chelenga, Hitoshi Chiba, Yojiro Yanagawa, Seiji Katagiri, Masashi Nagano, Shu-Ping Hui
Summary: Severe negative energy balance and high circulating free fatty acids in postpartum cows can impair the quality of oocytes. This study investigated the compositions of energy metabolism-related lipids in plasma and oocytes of cows at different lactation stages under intensive feeding management. The results showed that early postpartum cows had elevated levels of oocyte free fatty acids, indicating a high risk of lipotoxicity. The severe negative energy balance in the previous weeks was closely related to increased oocyte free fatty acid content.
Article
Public, Environmental & Occupational Health
Koshi Nakamura, Shu-Ping Hui, Shigekazu Ukawa, Emiko Okada, Takafumi Nakagawa, Akihiro Imae, Hiroaki Okabe, Zhen Chen, Yusuke Miura, Hitoshi Chiba, Akiko Tamakoshi
Summary: This study found a negative association between serum 25(OH)D3 levels and diabetes prevalence in a general Japanese population, with a slight inverse association between serum 25(OH)D3 levels and HOMA-IR.
JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Food Science & Technology
Zhen Chen, Nianqiu Shen, Xunzhi Wu, Jiaping Jia, Yue Wu, Hitoshi Chiba, Shuping Hui
Summary: This study compared and optimized methods for extracting phytosterols from edible seaweeds and established a quantitation method using H-1 NMR for total sterols and major sterol components. The phytosterol content in three common edible seaweeds from Japan was compared, and Hijiki showed the highest total sterol amount, significantly higher than Wakame and Kombu.
Article
Multidisciplinary Sciences
Zhen Chen, Shuji Sai, Kiyoshi Nagumo, Yue Wu, Hitoshi Chiba, Shu-Ping Hui
Summary: By analyzing serum samples from 20 patients with Kawasaki disease, it was found that lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) were decreased in the IVIG-resistant group before treatment, suggesting their potential as markers for diagnosing IVIG resistance. During treatment, the lipidomic changes were weaker in the IVIG-resistant group. After treatment, LPC and LPE species were lower in the IVIG-resistant group and negatively correlated with inflammatory markers, indicating the potential association between lipid metabolism and IVIG sensitivity/resistance in Kawasaki disease.
Article
Food Science & Technology
Zhen Chen, Xiaofang Lian, Meichen Zhou, Xiuli Zhang, Cong Wang
Summary: Cystine-enriched food supplements lack industry standards and market regulations, leading to issues such as food adulteration and fraud. This study has developed a reliable method using quantitative NMR to determine cystine in food supplements and additives. The method exhibits higher sensitivity, precision, and reproducibility than conventional titrimetric methods, and is more straightforward and economical than HPLC and LC-MS. The findings of this study highlight the need for standardization and regulation in the cystine supplement market.