Article
Biophysics
Esra Yuca, Ebru Sahin Kehribar, Urartu Ozgur Safak Seker
Summary: The study revealed that CsgA and CsgB exhibit varying binding strengths on different surfaces, with CsgB being crucial for determining the adhesiveness of the CsgA-CsgB mixture on hydroxyapatite and silica surfaces, and CsgA controlling the behavior of the mixture on gold surfaces. These findings offer insights into the binding behavior of curli proteins on different surfaces, aiding in achieving more precise control over their adhesion to targeted surfaces.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Md Nadir Hassan, Faisal Nabi, Asra Nasir A. Khan, Murtaza N. Hussain, Waseem A. Siddiqui, Vladimir N. Uversky, Rizwan Hasan Khan
Summary: Proteins and their aggregation are important in the study of neurodegenerative diseases and have both disease-related and functional roles. This review discusses the role of amyloids in diseases like Alzheimer's and Parkinson's, as well as their functional roles in various biological processes. The transformation of amyloids from being considered harmful to being recognized as having beneficial functions is also explored.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Daniel E. Otzen, Morten S. Dueholm, Zahra Najarzadeh, Tuomas P. J. Knowles, Francesco Simone Ruggeri
Summary: The formation of amyloid structures is not only linked to human diseases, but also plays a crucial role in stabilizing cellular environments in animals and bacteria. Infrared Nanospectroscopy (AFM-IR) has been successfully utilized to study functional amyloids, identifying amyloid proteins in single intact cells and providing nanoscale-resolved chemical images and spectra of purified amyloids. AFM-IR is demonstrated to be a new and powerful label-free tool for in situ investigations of the biophysical state of functional amyloid and biomolecules in general.
Article
Food Science & Technology
Claudia Ibacache-Quiroga, Karoll Gonzalez-Pizarro, Mariam Charifeh, Christian Canales, Rodrigo Diaz-Viciedo, Oliver Schmachtenberg, M. Alejandro Dinamarca
Summary: This study characterizes two Chilean kefir beverages, K02 and K03, through metagenomic and functional analysis. The results reveal the potential health benefits and biotechnological applications of these beverages, as well as their ability to inhibit biofilm formation of pathogenic bacteria.
Article
Biochemistry & Molecular Biology
Natalia Szulc, Marlena Gasior-Glogowska, Jakub W. Wojciechowski, Monika Szefczyk, Andrzej M. Zak, Michal Burdukiewicz, Malgorzata Kotulska
Summary: The study found that the self-aggregation properties of CsgA repeat fragments differ between Salmonella enterica and Escherichia coli, with Salmonella enterica showing easier and more durable formation of amyloid structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Mohd W. W. Azam, Asad U. U. Khan
Summary: This study demonstrates that knocking down the csgD gene can reduce amyloid and biofilm formation, as well as suppress the expression of genes associated with pathogenicity.
FRONTIERS IN NUTRITION
(2022)
Review
Microbiology
Nir Salinas, Tatyana L. Povolotsky, Meytal Landau, Ilana Kolodkin-Gal
Summary: Biofilms are multicellular communities of bacteria held together by extracellular matrix containing amyloid fibers, which play important roles in microbial physiology and virulence. Bacterial amyloid proteins have diverse nonscaffold functions, such as bridging cells during collective migration, regulating cell fate, and modulating the host's immune system.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Chemistry, Physical
Tessa Gilmore, Pelagia-Irene Gouma
Summary: This perspective discusses two types of functional nanomaterials, amyloid fibrils and metal oxide nanowires and nanogrids, which rely on their polymorphism to exhibit important properties in sensing and catalysis.
Review
Infectious Diseases
Leticia Matilla-Cuenca, Alejandro Toledo-Arana, Jaione Valle
Summary: Amyloids play a significant role in bacterial biofilms, serving as potential targets for drug development to enhance therapeutic strategies against biofilm-related infections.
Article
Multidisciplinary Sciences
Hema M. Swasthi, Joseph L. Basalla, Claire E. Dudley, Anthony G. Vecchiarelli, Matthew R. Chapman
Summary: The authors demonstrate that the phase separation of the cell surface-associated protein CsgF is crucial for the secretion and assembly of amyloidogenic curli subunits. CsgF is important for the proper assembly of curli, and its ability to phase-separate is correlated with its function. The formation of multicomponent CsgF-B condensates is proposed to facilitate amyloid formation on the cell surface.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Thorbjorn Vincent Sonderby, Zahra Najarzadeh, Daniel Erik Otzen
Summary: Functional amyloid is produced by various organisms, particularly well-understood in bacteria. Its formation is influenced by repeat sequences and helper proteins. Smart peptides and small molecules can be used to reduce biofilm formation. Additionally, functional amyloid can form two-dimensional films on structured surfaces, suggesting potential applications in biosensors and nanobiomaterials.
Article
Biochemistry & Molecular Biology
Sujeet S. Bhoite, Divya Kolli, Mark A. Gomulinski, Matthew R. Chapman
Summary: Bacterial biofilm formation can be influenced by the charge state of gatekeeper residues in the CsgA protein, which is a major component of curli amyloid fibers produced by enteric bacteria. A mechanism of gatekeeping is proposed where certain arginine and lysine residues interact with negatively charged aspartic acid residues, tempering CsgA fiber formation. This finding expands our understanding of the factors that regulate biofilm formation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Baizhu Chen, Wei Kang, Jing Sun, Runtao Zhu, Yue Yu, Aiguo Xia, Mei Yu, Meng Wang, Jinyu Han, Yixuan Chen, Lijun Teng, Qiong Tian, Yin Yu, Guanglin Li, Lingchong You, Zhiyuan Liu, Zhuojun Dai
Summary: The study successfully demonstrated the programmed self-assembly of bacterial populations using engineered adhesion, resulting in the development of versatile, programmable, self-healing living materials. The materials have fast recovery capabilities and can be used to create wearable sensors for detecting bio signals.
NATURE CHEMICAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Dingran Chang, Sandy Zakaria, Sahar Esmaeili Samani, Yangyang Chang, Carlos D. M. Filipe, Leyla Soleymani, John D. Brennan, Meng Liu, Yingfu Li
Summary: FNA-based biosensors show great potential in detecting pathogens with high sensitivity directly in clinical samples, providing tremendous potential for clinical applications.
ACCOUNTS OF CHEMICAL RESEARCH
(2021)
Review
Microbiology
Khushal Khambhati, Jaykumar Patel, Vijaylaxmi Saxena, A. Parvathy, Neha Jain
Summary: Biofilms are bacterial communities enclosed in a dynamic extracellular matrix, with environmental factors playing a crucial role in their transition from planktonic to sessile form. Amyloids, proteinaceous polymers, are major components of the biofilm matrix, regulating adhesion, aggregation, and infection. The expression and assembly of amyloids in bacteria are spatially and temporally regulated, with genetic control ensuring optimal utilization during biofilm formation.
Article
Biology
Alan Tran, Angelina Tang, Colleen T. O'Loughlin, Anthony Balistreri, Eric Chang, Doris Coto Villa, Joy Li, Aruna Varshney, Vanessa Jimenez, Jacqueline Pyle, Bryan Tsujimoto, Christopher Wellbrook, Christopher Vargas, Alex Duong, Nebat Ali, Sarah Y. Matthews, Samantha Levinson, Sarah Woldemariam, Sami Khuri, Martina Bremer, Daryl K. Eggers, Noelle L'Etoile, Laura C. Miller Conrad, Miri K. VanHoven
Article
Microbiology
Anthony Balistreri, Ethan Kahana, Soorya Janakiraman, Matthew R. Chapman
FRONTIERS IN MICROBIOLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Laura C. Miller Conrad, Alan Tran, Angelina Tang, Colleen T. O'Loughlin, Anthony Balistreri, Eric Chang, Doris Coto Villa, Joy Li, Aruna Varshney, Sarah Matthews, Martina Bremmer, Miri K. VanHoven
Meeting Abstract
Chemistry, Multidisciplinary
Terrence Nguyen, Anthony Balistreri, Matthew Aguilar, Arturo Chavez, Mellanie Gomes, Alexander Jiu, Laura Miller Conrad
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Radiology, Nuclear Medicine & Medical Imaging
Sebastian Obrzut, Kevin McCammack, Karam W. Badran, Anthony Balistreri, Evan Ou, Brian J. Nguyen, Carl K. Hoh, Steven C. Rose