Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Cyril Bouland, Pierre Philippart, Didier Dequanter, Florent Corrillon, Isabelle Loeb, Dominique Bron, Laurence Lagneaux, Nathalie Meuleman
Summary: Bone regeneration is a complex process relying on interactions between osteogenesis and angiogenesis. Tissue engineering provides strategies with regenerative cell sources, growth factors, and mechanical stimulation. Vascularization is crucial for bone formation and function, with studies showing synergy between mesenchymal stromal cells (MSCs) and endothelial progenitor cells (EPCs) in promoting regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Mehdi Hassanpour, Amankeldi A. Salybekov, Shuzo Kobayashi, Takayuki Asahara
Summary: CD34 is a cell surface antigen expressed in stem/progenitor cells such as HSCs and EPCs, which are potential sources for regenerative therapy. CD34(+) cells have been shown to improve therapeutic angiogenesis through direct incorporation and paracrine activity. Preclinical, pilot, and clinical trials have demonstrated the safety and efficacy of CD34(+) cell therapy in various diseases. However, the clinical application of CD34(+) cell therapy has sparked debates and controversies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Ross E. B. Fitzsimmons, Ronald G. Ireland, Aileen Zhong, Agnes Soos, Craig A. Simmons
Summary: One approach to engineering viable tissues ex vivo involves using hydrogel-based microfluidics to generate microvessels, with a focus on collagen, fibrin, and collagen-fibrin co-gel formulations. Fibrin and co-gels were found to promote multicellular EC sprouting, while collagen required supplementation to elicit a migration response. Increasing collagen content in co-gel formulations led to higher compressive modulus and reliable formation of intact hydrogel-based microchannels for perfusion, making collagen-fibrin co-gels a promising option for generating vascularized tissue constructs.
BIOMEDICAL MATERIALS
(2021)
Review
Oncology
Ghazaleh Hashemi, James Dight, Kiarash Khosrotehrani, Laura Sormani
Summary: Melanoma is an aggressive and potentially lethal form of skin cancer. The role of blood vessel formation, particularly endothelial progenitor cells (EPCs), in melanoma is of significance. Understanding the role of EPCs is important for understanding the abnormal vessel structures in melanoma. The effect of anti-angiogenic drugs on melanoma treatment needs further research.
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biology
Jinnan Xiang, Jigang Guo, Shaoyang Zhang, Hongguang Wu, Ye-Guang Chen, Junping Wang, Baojie Li, Huijuan Liu
Summary: This study found that the Twist2 stromal lineage cells play a crucial role in the growth and maintenance of intestinal stem cells (ISCs) and their ability to regenerate villi. Additionally, Acta2(+) cells also contribute to ISC regeneration. These findings suggest that different cellular niches are required for ISC-mediated villus homeostasis and regeneration.
Article
Multidisciplinary Sciences
Ningbo Wu, Hongxiang Sun, Xiaoyun Zhao, Yao Zhang, Jianmei Tan, Yuanyuan Qi, Qun Wang, Melissa Ng, Zhaoyuan Liu, Lingjuan He, Xiaoyin Niu, Lei Chen, Zhiduo Liu, Hua-Bing Li, Yi Arial Zeng, Manolis Roulis, Dou Liu, Jinke Cheng, Bin Zhou, Lai Guan Ng, Duowu Zou, Youqiong Ye, Richard A. Flavell, Florent Ginhoux, Bing Su
Summary: The study identified a subset of intestinal stromal cells, MRISCs, as the primary cellular source of WNT agonist R-spondin 1 following intestinal injury in mice. These MRISCs are located at the bases of colon crypts, maintain LGR5(+) intestinal stem cells, and protect against acute intestinal damage through enhanced R-spondin 1 production. The mechanism involves a reactive oxygen species (ROS)-MAP3K2-ERK5-KLF2 axis to augment WNT signaling for intestinal regeneration.
Article
Immunology
Ana Merino, Marta Sablik, Sander S. Korevaar, Carmen Lopez-Iglesias, Maitane Ortiz-Virumbrales, Carla C. Baan, Eleuterio Lombardo, Martin J. Hoogduijn
Summary: This study found that membrane particles (MP) generated from mesenchymal stromal cells (MSC) can preserve the beneficial effects of MSC on endothelial cell repair processes. This suggests that MP may have potential applications for treating vascular diseases where inflammatory processes damage the endothelium.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell & Tissue Engineering
Len Frisbie, Ronald J. Buckanovich, Lan Coffman
Summary: The interaction between tumor cells and non-malignant host cells within the tumor microenvironment (TME) is crucial for cancer progression. Mesenchymal stem/stromal cells (MSCs), one of the non-malignant host cells, play a critical role in the formation and function of the TME. These MSCs, when reprogrammed by cancer cells, become carcinoma-associated MSCs (CA-MSCs) and contribute to tumor growth, metastasis, and TME formation. CA-MSCs can differentiate into various stromal sub-lineages, secrete extracellular matrix, promote angiogenesis, and recruit immunosuppressive myeloid cells, thereby impacting almost every aspect of the TME. However, the heterogeneity of CA-MSCs and the lack of definitive markers for their identification pose challenges in accurately understanding their origin and characteristics.
Article
Anatomy & Morphology
Pratheesh Mankuzhy, Arun Dharmarajan, Lakshmi R. Perumalsamy, Khan Sharun, Priyanka Samji, Rodney J. Dilley
Summary: This passage introduces the complex process of blood vessel development, growth, and remodeling, as well as the signaling pathways involved in this process. It highlights the potential of resident and exogenous mesenchymal stromal cells (MSCs) to regulate neovascularization through the secretion of proangiogenic factors. The review provides an updated overview of the canonical and non-canonical Wnt signaling pathways, as well as recent studies on MSCs' involvement in vasculogenic processes mediated by Wnt signaling pathways.
Article
Engineering, Biomedical
Johnny Lam, Byungjun Lee, James Yu, Brian J. Kwee, Yangji Kim, Jiho Kim, Yeongmin Choi, Jun Sung Yoon, Youngsoo Kim, Kyusuk Baek, Noo Li Jeon, Kyung E. Sung
Summary: Mesenchymal stromal cells (MSCs) have therapeutic potential in influencing endogenous regenerative processes, such as vasculogenesis. However, the functional heterogeneity of MSCs and the challenge of quality control pose difficulties in clinical applications. A novel bioassay based on a high-throughput microphysiological system was developed to measure the specific bioactivity of MSCs in stimulating vasculogenesis. The study found significant heterogeneity in vasculogenic potency among MSCs from different donors and cell passages, and this difference was correlated with the baseline expression of genes associated with vasculogenesis. These findings highlight the significant functional heterogeneity of MSCs and suggest the importance of baseline gene expression in determining their bioactivity.
Review
Cell Biology
Susan L. Lindsay, Susan C. Barnett
Summary: The use of MSCs for transplant-mediated repair after SCI is promising, but different tissue sources of MSCs may have varying biological properties. The importance of identifying the appropriate niche-specific MSC type for SCI repair is highlighted.
Article
Biology
Hyunsook Lee, Yang-Hoon Huh, Kyu-Tae Kang
Summary: This study investigated the role of mesenchymal stem cells (MSCs) in enhancing the vasculogenic capacity of endothelial colony-forming cells (ECFCs) under high-glucose conditions. The researchers found that the combination of ECFCs and MSCs restored the decreased tube formation ability of ECFCs in high-glucose conditions. In vivo experiments using diabetic mice showed that the combined delivery of ECFCs and MSCs led to the formation of perfused blood vessels in diabetic ischemic regions.
Article
Engineering, Biomedical
Shuang Zhang, Bastiaan Tuk, Jeroen Van de Peppel, Gert-Jan Kremers, Marijke Koedam, Georg R. Pesch, Zaid Rahman, Remco M. Hoogenboezem, Eric M. J. Bindels, Johan W. Van Neck, Pouyan E. Boukany, Johannes P. T. M. Van Leeuwen, Bram C. J. Van der Eerden
Summary: A functional vascular system is crucial for bone repair, and disrupted angiogenesis can lead to non-union. Paracrine factors released by human bone marrow derived mesenchymal stromal cells (BM-SCs) have angiogenic effects on endothelial cells. This study used microfluidic designs to investigate angiogenesis and found that BMSC conditioned medium (CM) significantly promoted endothelial cell proliferation, chemotactic and mechanotactic migration. Transcriptional analysis of endothelial cells revealed unique gene expression related to tricarboxylic acid cycle and energy metabolism when exposed to both BMSC-CM and shear stress. This study highlights the importance of considering in vivo-like microenvironments when studying vessel repair during fracture healing.
ACTA BIOMATERIALIA
(2022)
Article
Neurosciences
Thomas Haider, Romana Hoeftberger, Beate Rueger, Michael Mildner, Roland Blumer, Andreas Mitterbauer, Tanja Buchacher, Camillo Sherif, Patrick Altmann, Heinz Redl, Christian Gabriel, Mariann Gyoengyoesi, Michael B. Fischer, Gert Lubec, Hendrik Jan Ankersmit
EXPERIMENTAL NEUROLOGY
(2015)
Article
Physiology
Beate M. Rueger, Tanja Buchacher, Alexander Giurea, Bernd Kubista, Michael B. Fischer, Johannes M. Breuss
FRONTIERS IN PHYSIOLOGY
(2018)
Article
Hematology
Anita Schildberger, Tanja Buchacher, Viktoria Weber, Dieter Falkenhagen
BLOOD PURIFICATION
(2011)
Article
Immunology
Tanja Buchacher, Herbert Wiesinger-Mayr, Klemens Vierlinger, Beate M. Rueger, Gerold Stanek, Michael B. Fischer, Viktoria Weber
Article
Multidisciplinary Sciences
Tanja Buchacher, Anna Ohradanova-Repic, Hannes Stockinger, Michael B. Fischer, Viktoria Weber
Article
Biotechnology & Applied Microbiology
Nadja Jessberger, Markus Kranzler, Claudia Da Riol, Valerie Schwenk, Tanja Buchacher, Richard Dietrich, Monika Ehling-Schulz, Erwin Maertlbauer
Article
Chemistry, Multidisciplinary
Ankitha Shetty, Santosh D. Bhosale, Subhash Kumar Tripathi, Tanja Buchacher, Rahul Biradar, Omid Rasool, Robert Moulder, Sanjeev Galande, Riitta Lahesmaa
Summary: Dysregulated function of Th17 cells is associated with immunodeficiencies and autoimmune disorders. FOSL1 and FOSL2 modulate the effector functions of Th17 cells, with new mechanistic links discovered through the first interactomes study in human Th17 cells.
Article
Cell Biology
Partho Sen, Syed Bilal Ahmad Andrabi, Tanja Buchacher, Mohd Moin Khan, Ubaid Ullah Kalim, Tuomas Mikael Lindeman, Marina Amaral Alves, Victoria Hinkkanen, Esko Kemppainen, Alex M. Dickens, Omid Rasool, Tuulia Hyotylainen, Riitta Lahesmaa, Matej Oresic
Summary: The study demonstrates specific metabolic changes in human CD4(+) T cells during activation and functional differentiation, highlighting the importance of the de novo sphingolipid pathway in Th17 cell differentiation and effector functions, with serine palmitoyltransferase playing a significant role in the expression of proinflammatory cytokines IL-17A and IL17F by Th17 cells. These findings offer a comprehensive resource for selectively manipulating CD4(+) T cells in conditions characterized by an imbalance of Th17/natural Treg (nTreg) cells.
Article
Multidisciplinary Sciences
Tanja Buchacher, Anni Honkimaa, Tommi Valikangas, Niina Lietzen, M. Karoliina Hirvonen, Jutta E. Laiho, Amir-Babak Sioofy-Khojine, Eeva-Liisa Eskelinen, Heikki Hyoty, Laura L. Elo, Riitta Lahesmaa
Summary: Enteroviruses, especially group B coxsackieviruses (CVBs), have been linked to the development of type 1 diabetes. However, the mechanisms behind persistent enterovirus infection and beta cell autoimmunity are not fully understood. This study established a persistent infection model using two CVB1 strains in a human pancreatic cell line and observed changes in gene expression related to the pancreatic microenvironment, secretory pathway, and lysosomal biogenesis. The antiviral response pathways were also found to be differently activated by the two strains.
Article
Biochemistry & Molecular Biology
Ankitha Shetty, Subhash Kumar Tripathi, Sini Junttila, Tanja Buchacher, Rahul Biradar, Santosh D. Bhosale, Tapio Envall, Asta Laiho, Robert Moulder, Omid Rasool, Sanjeev Galande, Laura L. Elo, Riitta Lahesmaa
Summary: This study characterizes the human-specific roles of three AP-1 transcription factors, FOSL1, FOSL2, and BATF, during early stages of Th17 differentiation. The findings show that FOSL1 and FOSL2 co-repress Th17 fate-specification, while BATF promotes the Th17 lineage. The study also reveals that the genomic binding sites of these transcription factors harbor autoimmune disease-linked SNPs.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Tanja Buchacher, Astrid Digruber, Markus Kanzler, Giorgia Del Favero, Monika Ehling-Schulz
Summary: This study reveals the presence of virulence-associated factors in extracellular vesicles (EVs) from enteropathogenic Bacillus cereus, which are delivered to host cells through membrane fusion and endocytosis, leading to cellular toxicity and inflammatory response. These findings provide new insights into molecular processes involved in disease development.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Niina Lietzen, Karoliina Hirvonen, Anni Honkimaa, Tanja Buchacher, Jutta E. Laiho, Sami Oikarinen, Magdalena A. Mazur, Malin Flodstrom-Tullberg, Eric Dufour, Amir-Babak Sioofy-Khojine, Heikki Hyoty, Riitta Lahesmaa