Article
Biotechnology & Applied Microbiology
Bo Chen, Ying Deng, Bo Wang, Zhongyi Tian, Jindong Tong, Bo Yu, Weijun Shi, Jingdong Tang
Summary: Thromboangiitis obliterans (TAO) is a vascular inflammatory disease, and this study identified differentially expressed lncRNAs and miRNAs in TAO patients. A ceRNA network associated with TAO was constructed, revealing a potential regulatory network involving NEAT1/miR-1-3p/GNA12 signaling in TAO progression. Dual luciferase reporter assays confirmed the interaction between NEAT1, miR-1-3p, and GNA12.
Article
Biotechnology & Applied Microbiology
Kun Du, Xue Bai, Li Chen, Yu Shi, Hao-ding Wang, Ming-cheng Cai, Wen-qiang Sun, Jie Wang, Shi-yi Chen, Xian-bo Jia, Song-jia Lai
Summary: This study revealed the ceRNA networks during BAT whitening in rabbits, providing new insights into BAT-based obesity treatments.
Review
Neurosciences
Mohammad Reza Asadi, Samin Abed, Ghazal Kouchakali, Fateme Fattahi, Hani Sabaie, Marziyeh Sadat Moslehian, Mirmohsen Sharifi-Bonab, Bashdar Mahmud Hussen, Mohammad Taheri, Soudeh Ghafouri-Fard, Maryam Rezazadeh
Summary: Parkinson's disease (PD) is a unique clinical syndrome with multiple causes and clinical manifestations. Research on ceRNA axes is increasing, highlighting their importance in PD. Understanding these axes may help elucidate the causes of PD and provide innovative therapeutic targets.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Oncology
Soudeh Ghafouri-Fard, Arash Safarzadeh, Bashdar Mahmud Hussen, Mehdi Akhavan-Bahabadi, Mohammad Taheri, Guive Sharifi
Summary: This study constructed putative ceRNA networks based on expression profiles of GBM tissues and identified differentially expressed mRNAs, lncRNAs, and miRNAs, as well as 10 hub genes in the ceRNA network. This provides novel insights into molecular events during GBM pathogenesis and potential therapeutic targets for GBM.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Dulari K. Jayarathna, Miguel E. Renteria, Jyotsna Batra, Neha S. Gandhi
Summary: This study identified stage-specific competing endogenous RNAs (ceRNAs) in breast cancer using cancer genome atlas data and investigated their molecular functions and prognostic ability. Five genes and one long non-coding RNA were found to be unique to the stage IV ceRNA network, and these genes have been described in previous breast cancer studies. Survival analysis identified prognostic microRNAs in stages I, II, and III. These findings reveal a set of unique ceRNAs in metastatic breast cancer, and further experimental work is needed to evaluate their role in metastasis. Identifying stage-specific ceRNAs will also contribute to the understanding of personalized therapeutics in breast cancer.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Yanjie Zhao, Heng Zhang, Qiang Ju, Xinmei Li, Yuxin Zheng
Summary: This study identified hundreds of RNAs related to the survival time of gastric cancer patients and constructed a survival-related network comprising lncRNAs, miRNAs, and mRNAs. Eight RNAs associated with prognosis were used to build prognostic prediction models.
FRONTIERS IN GENETICS
(2021)
Article
Neurosciences
Natalia Gil-Jaramillo, Andres Felipe Aristizabal-Pachon, Maria Alejandra Luque Aleman, Valentina Gonzalez Gomez, Hans Deyvy Escobar Hurtado, Laura Camila Giron Pinto, Juan Sebastian Jaime Camacho, Alexis Felipe Rojas-Cruz, Yeimy Gonzalez-Giraldo, Andres Pinzon, Janneth Gonzalez
Summary: Neurodegenerative diseases (NDs) are characterized by progressive deterioration of neuronal function, leading to motor and cognitive damage in patients. This study presents a comprehensive competing endogenous RNA (ceRNA) network to explore the key regulatory mechanisms related to NDs, using transcriptomic data and experimentally validated non-coding RNA (ncRNA) interactions. The findings provide insights into the pathogenesis of NDs and potential targets for diagnostic molecules and pharmacologic treatments.
FRONTIERS IN NEUROSCIENCE
(2023)
Review
Oncology
Qian Bai, Zhenjie Pan, Ghulam Nabi, Farooq Rashid, Yang Liu, Suliman Khan
Summary: Thyroid cancer is the most common endocrine malignancy, and non-coding RNAs play important roles in its development. Competing endogenous RNA is a mechanism of regulatory interactions, and multiple ceRNA networks are deregulated in thyroid cancer development, metastasis, and drug resistance. Further research on these deregulations is needed for early detection and effective treatment of thyroid cancer.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Psychiatry
Rongjie Li, Qiaoye Wang, Yufen Qiu, Youshi Meng, Lei Wei, Hao Wang, Ruikang Mo, Donghua Zou, Chunbin Liu
Summary: This study investigated the relationship among ceRNA, autophagy, and Schizophrenia by analyzing gene expression profiles of SZ patients. Enrichment analysis revealed associations with cellular responses to oxygen concentration and pathways such as ErbB, AMPK, and mTOR signaling. The study also constructed an autophagy-related ceRNA network and found high diagnostic efficiency of miRNA in SZ, particularly hsa-miR-423-5p and hsa-miR-4532.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Mauro Esposito, Nicolo Gualandi, Giovanni Spirito, Federico Ansaloni, Stefano Gustincich, Remo Sanges
Summary: L1 LINEs are transposable elements that can replicate within the genome. They may have been positively selected for beneficial cellular functions, but their deregulation can lead to diseases. miRNAs and ceRNAs regulate transcript levels in somatic cells. This study found that L1s may act as ceRNAs and help interpret transcriptomic responses.
Article
Neurosciences
Gengjing Fang, Yuling Zhou, Xiaopeng Zhou, Hui Zhou, Yuan-Yuan Ge, Shengtao Luo, Jiang-Fan Chen, Liping Zhang
Summary: The study revealed that A(2A)R antagonist KW6002 partially reversed CCCP-induced retinal injury by increasing mitochondrial content and reducing cell apoptosis. Transcriptome sequencing showed that A(2A)R blockade decreased the number of CCCP-induced microRNAs by 60% and increased circular RNAs by 50%. These findings provide insights into the neuroprotective mechanisms of A(2A)R antagonists against CCCP-induced retinal injury.
Article
Cell Biology
Jie Liu, Zhenxing Ren, Lin Yang, Lulu Zhu, Yi li, Caiqun Bie, Helu Liu, Yichun Ji, Dongfeng Chen, Meiling Zhu, Weihong Kuang
Summary: This study demonstrates that the NSUN5-FTH1/FTL pathway mediates ferroptosis in BMSCs by regulating GPX4 and ROS accumulation, potentially affecting the survival of transplanted BMSCs.
CELL DEATH DISCOVERY
(2022)
Review
Genetics & Heredity
Liang Cen, Ruochen Liu, Wei Liu, Qianqian Li, Hongjuan Cui
Summary: The ceRNA mechanism plays a crucial role in gliomas, with dysregulated networks leading to abnormal expression of cancer driver genes and the establishment of glioma hallmarks. These findings open up new avenues for understanding gliomas and provide potential new targets for treatment.
FRONTIERS IN GENETICS
(2021)
Review
Oncology
Median Zhao, Jianguo Feng, Liling Tang
Summary: CeRNAs can regulate biological processes through complex networks, potentially leading to cancer when imbalanced; Dysregulated RNAs in lung cancer may serve as ceRNAs to regulate various biological functions; CeRNAs are also being explored as potential prognostic and diagnostic biomarkers in lung cancer.
CANCER BIOLOGY & MEDICINE
(2021)
Article
Biology
Luca Tirinato, Maria Grazia Marafioti, Francesca Pagliari, Jeannette Jansen, Ilenia Aversa, Rachel Hanley, Clelia Nistico, Daniel Garcia-Calderon, Geraldine Genard, Joana Filipa Guerreiro, Francesco Saverio Costanzo, Joao Seco
Summary: The study identified that radioresistant cancer cells in breast, bladder, lung, neuroglioma, and prostate showed an increase in lipid droplet (LD) number, with cells containing higher LDs exhibiting greater clonogenic potential after irradiation. The researchers found a close connection between LD content and iron metabolism, particularly with the presence of the ferritin heavy chain (FTH1). Silencing the FTH1 gene in breast and lung cancer cells reduced LD numbers and increased radiosensitivity, while FTH1 overexpression and iron-chelating treatment restored LD levels and radioresistance.
Article
Dentistry, Oral Surgery & Medicine
Flavia Biamonte, Caterina Buffone, Gianluca Santamaria, Anna Martina Battaglia, Chiara Mignogna, Leonzio Fortunato, Francesco Saverio Costanzo, Amerigo Giudice
Summary: The study confirmed the existence of molecular divergence from the center of the lesion compared to white light and VELscope(TM) defined margins in leukoplakia and oral carcinoma patients. The white light margin retained molecular abnormalities observed in the center of the lesion, suggesting the presence of a partially transformed cell population. Combination of autofluorescence with conventional oral examination was found beneficial in identifying surgical margins during biopsy procedures for leukoplakia and oral carcinoma, despite the limited number of patients in the study.
Article
Oncology
Cirino Botta, Alessia Indrieri, Eugenio Garofalo, Flavia Biamonte, Andrea Bruni, Pino Pasqua, Francesco Cesario, Francesco Saverio Costanzo, Federico Longhini, Francesco Mendicino
Summary: Since the outbreak of SARS-CoV-2 in late 2019, the global health system has been severely impacted, resulting in increased hospitalization rates, ICU access, and mortality rates, mainly due to severe acute respiratory failure and multi-organ failure. The excessive release of proinflammatory cytokines and the potential benefits of immunotherapy in the treatment of COVID-19 patients are highlighted. Targeting Janus Kinase (JAK) signaling may be a promising approach to limit the detrimental effects of an overwhelming inflammatory response in COVID-19 patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Flavia Biamonte, Cirino Botta, Maria Mazzitelli, Salvatore Rotundo, Enrico Maria Trecarichi, Daniela Foti, Carlo Torti, Giuseppe Viglietto, Daniele Torella, Francesco Costanzo
Summary: Through a retrospective evaluation of laboratory parameters in 45 elderly subjects, three laboratory response patterns were identified, strongly associated with patients' survival. Key markers such as D-dimer, iron status, and lymphocyte/monocyte count were found to play significant roles in predicting clinical progression in COVID-19 patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Cell Biology
Alessandro Sacco, Anna Martina Battaglia, Cirino Botta, Ilenia Aversa, Serafina Mancuso, Francesco Costanzo, Flavia Biamonte
Summary: New insights have been gained into the impact of iron metabolism within the tumor microenvironment on cancer development. Iron promotes the production of reactive oxygen species, which can trigger cell death or malignant transformation, affecting tumor immunosurveillance. The potential and controversies of using iron metabolism as a target for anticancer treatments are discussed, particularly in the context of hot and cold tumors.
Article
Biochemistry & Molecular Biology
Elena Cesaro, Arianna Pastore, Alessia Polverino, Lorenzo Manna, Giuseppina Divisato, Cristina Quintavalle, Maddalena Di Sanzo, Maria Concetta Faniello, Michela Grosso, Paola Costanzo
Summary: The zinc finger protein ZNF224 has been found to play a role in promoting tumorigenic properties in melanoma, including cell proliferation and invasiveness. ZNF224 modulates the expression of TGF-beta itself and its receptors, possibly enhancing endogenous TGF beta/Smad signaling and promoting epithelial-mesenchymal transition and tumor metastasis.
HUMAN MOLECULAR GENETICS
(2021)
Article
Cell Biology
Luana Scaramuzzino, Valeria Lucchino, Stefania Scalise, Michela Lo Conte, Clara Zannino, Alessandro Sacco, Flavia Biamonte, Elvira Immacolata Parrotta, Francesco Saverio Costanzo, Giovanni Cuda
Summary: The study investigated how embryonic stem cells respond to iron accumulation following stable knockdown of the FTH1 gene. While silencing FTH1 does not lead to increased ROS production or changes in redox status, it does affect the differentiation potential of ESCs and induces apoptosis. Interestingly, silencing FTH1 activates the Nrf2 signaling pathway and PPP, highlighting how ESCs combat oxidative stress through these mechanisms.
Article
Oncology
Andrea Fulco, Francesco Chiaradia, Luigi Ascalone, Vincenzo Andracchio, Antonio Greco, Manlio Cappa, Marcello Scarcia, Giuseppe Mario Ludovico, Vincenzo Pagliarulo, Camillo Palmieri, Stefano Alba
Summary: The study compared the diagnostic accuracy of multiparametric MRI with fusion ultrasound-guided prostate biopsy and standard biopsy, showing that the combined targeted and standard biopsy approach can reduce the risk of missing clinically significant prostate cancer.
Article
Microbiology
Carmela De Marco, Claudia Veneziano, Alice Massacci, Matteo Pallocca, Nadia Marascio, Angela Quirino, Giorgio Settimo Barreca, Aida Giancotti, Luigia Gallo, Angelo Giuseppe Lamberti, Barbara Quaresima, Gianluca Santamaria, Flavia Biamonte, Stefania Scicchitano, Enrico Maria Trecarichi, Alessandro Russo, Daniele Torella, Aldo Quattrone, Carlo Torti, Giovanni Matera, Caterina De Filippo, Francesco Saverio Costanzo, Giuseppe Viglietto
Summary: This study reports on the surveillance of SARS-CoV-2 in Southern Italy from March 2021 to February 2022. The study identified the circulating variants in the region and their dynamics over time, with Delta and Omicron becoming the dominant variants. The study also analyzed the distribution of these variants across Europe and different regions of Italy and predicted the effects of specific mutations on the host immune response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Salvatore Rotundo, Eleonora Vecchio, Antonio Abatino, Caterina Giordano, Serafina Mancuso, Maria Teresa Tassone, Chiara Costa, Alessandro Russo, Enrico Maria Trecarichi, Giovanni Cuda, Francesco Saverio Costanzo, Camillo Palmieri, Carlo Torti
Summary: This study found that short-term memory T-cell responses were not significantly hindered in COVID-19 patients after receiving neutralizing monoclonal antibody treatment. Different moAbs may have diverse impacts on the evolution of S-specific T-cell responses in vaccinated and unvaccinated patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Maddalena Di Sanzo, Flora Cozzolino, Anna Martina Battaglia, Ilenia Aversa, Vittoria Monaco, Alessandro Sacco, Flavia Biamonte, Camillo Palmieri, Francesca Procopio, Gianluca Santamaria, Francesco Ortuso, Piero Pucci, Maria Monti, Maria Concetta Faniello
Summary: The study found that the interaction between FTH1 and PRDX6 in cancer cells can affect cell proliferation and migration, leading to a less invasive phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Claudia Veneziano, Nadia Marascio, Carmela De Marco, Barbara Quaresima, Flavia Biamonte, Enrico Maria Trecarichi, Gianluca Santamaria, Angela Quirino, Daniele Torella, Aldo Quattrone, Giovanni Matera, Carlo Torti, Caterina De Filippo, Francesco Saverio Costanzo, Giuseppe Viglietto
Summary: We investigated the evolution of SARS-CoV-2 spread in Calabria, Southern Italy, in 2022. Analysis of Omicron variants in Calabria revealed the prevalence of 10 different sub-lineages. In addition, Calabria Omicron presented non-canonical mutations in the S protein and non-structural proteins.
Article
Biology
Federica Scotto di Carlo, Sharon Russo, Francesc Muyas, Maria Mangini, Lorenza Garribba, Laura Pazzaglia, Rita Genesio, Flavia Biamonte, Anna Chiara De Luca, Stefano Santaguida, Katia Scotlandi, Isidro Cortes-Ciriano, Fernando Gianfrancesco
Summary: Profilin 1, encoded by PFN1, is a protein that plays a tumor suppressive role in various adenocarcinomas and pagetic osteosarcomas. However, its exact contribution to tumor development is not fully understood. This study shows that inactivation of Profilin 1 leads to multiple mitotic defects, resulting in chromosomal instability and genome rearrangements in pagetic osteosarcomas. Mechanistically, Profilin 1 is involved in regulating cell division and its deficiency impairs actin filament supply during cytokinesis.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Eugenio Garofalo, Flavia Biamonte, Camillo Palmieri, Anna Martina Battaglia, Alessandro Sacco, Eugenio Biamonte, Giuseppe Neri, Giulio Cesare Antico, Serafina Mancuso, Giuseppe Foti, Carlo Torti, Francesco Saverio Costanzo, Federico Longhini, Andrea Bruni
Summary: In this study, we evaluated the spike-specific immune response in patients with breakthrough SARS-CoV-2 infection after receiving two doses of the BNT162b2 mRNA vaccine. The results showed that severe COVID-19 patients requiring hospitalization had lower levels of spike-specific IFN gamma-releasing cells compared to mild-moderate patients and healthy subjects, despite similar antibody titers.