Article
Biochemistry & Molecular Biology
Ana Jurado-Flores, Luis C. Romero, Cecilia Gotor
Summary: Hydrogen sulfide-mediated signaling pathways play crucial roles in regulating physiological and pathophysiological processes in mammals and plants. Through quantitative proteomic analysis, this study identified a substantial number of persulfidated proteins in Arabidopsis roots, revealing alterations in persulfidation levels under nitrogen deprivation. These persulfidated proteins are involved in a wide range of biological functions, including primary metabolism, stress responses, growth and development, RNA translation, and protein degradation.
Article
Biotechnology & Applied Microbiology
He Liu, Jinjin Qiao, Jiaolei Shangguan, Jing Zhu
Summary: Nitrogen metabolism can regulate mycelial growth and secondary metabolism in Ganoderma lucidum. Glutamate oxaloacetate transaminase (GOT) isoenzymes, GOT1 and GOT2, were found in G. lucidum, and the silencing of got1 and got2 affects the ROS level and secondary metabolism. The results provide new insights into the effect of GOT on ROS and secondary metabolism in fungi.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Clinical Neurology
Tianchi Tang, Yang Liu, Min Yang, Ming Tu, Wanchun Zhu, Ming Chen
Summary: GOT1 is low-expressed in GBM, but patients with high expression have better prognosis. Overexpressed GOT1 inhibits glycolysis and malignant phenotypes of GBM cells, and is positively correlated with PC.
WORLD NEUROSURGERY
(2021)
Article
Chemistry, Medicinal
Qiqi Wu, Zhongya Sun, Zhifeng Chen, Jingqiu Liu, Hong Ding, Cheng Luo, Mingliang Wang, Daohai Du
Summary: Glutamate oxaloacetate transaminase 1 (GOT1) is crucial for glutamine metabolism and its suppression can inhibit tumor growth and prevent cancer development. A high-throughput screening assay was developed to discover GOT1 inhibitors, and hydralazine hydrochloride was identified as a potential inhibitor with significant inhibitory effects on GOT1 catalytic activity.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Viktor Kozich, Bernd C. Schwahn, Jitka Sokolova, Michaela Krizkova, Tamas Ditroi, Jakub Krijt, Youssef Khalil, Tomas Krizek, Tereza Vaculikova-Fantlova, Blanka Stiburkova, Philippa Mills, Peter Clayton, Kristyna Barvikova, Holger Blessing, Jolanta Sykut-Cegielska, Carlo Dionisi-Vici, Serena Gasperini, Angeles Garcia-Cazorla, Tobias B. Haack, Tomas Honzik, Pavel Jesina, Alice Kuster, Lucia Laugwitz, Diego Martinelli, Francesco Porta, Rene Santer, Guenter Schwarz, Peter Nagy
Summary: Regulation of H2S homeostasis in humans is not well understood. By studying patients with genetic defects, the importance of certain enzymes in H2S synthesis and catabolism was assessed. The findings suggest the presence of control and compensatory mechanisms in the maintenance of H2S homeostasis.
Article
Biochemistry & Molecular Biology
Aaron P. Landry, Joseph Roman, Ruma Banerjee
Summary: This review discusses the enzymes involved in H2S homeostasis, cystathionine beta-synthase and sulfide quinone oxidoreductase, and their structural perspectives on reaction mechanisms and regulation. Understanding these key enzymes is crucial for understanding H2S homeostasis and its dysregulation in disease.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Neurosciences
Haiming Li, Dainan Zhang, Xiaoze Wang, Shensheng Wang, Meng Xiao
Summary: Alzheimer's disease (AD) is a neurodegenerative disease that affects the elderly and causes cognitive impairment and memory decline. Glutamate excitotoxicity is a known factor in AD, and it has been found that glutamic-oxaloacetic transaminase (GOT) can reduce glutamate concentrations in the hippocampi of mice. This study investigated the effects of GOT on neurological function and protein expression in mice with AD. The results showed that GOT treatment reduced beta-amyloid protein levels, improved cognitive function, increased neuron and synapse numbers in the hippocampus, and increased SIRT1 expression while decreasing A beta 1-42 expression.
NEUROSCIENCE LETTERS
(2023)
Article
Medicine, Research & Experimental
Ravichand Palakurti, Nirupam Biswas, Sashwati Roy, Surya C. Gnyawali, Mithun Sinha, Kanhaiya Singh, Subhadip Ghatak, Chandan K. Sen, Savita Khanna
Summary: The a-tocotrienol (TCT) form of natural vitamin E is more effective than alpha-tocopherol (α-TOC) in preventing stroke. TCT improves blood perfusion at the stroke-affected site by inducing miR-1224, which has angiogenic properties. Inhibiting Serpine1, a target of miR-1224, enhances the beneficial effects of TCT on stroke outcomes.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Biochemistry & Molecular Biology
Paraskevi-Maria Psefteli, Phoebe Kitscha, Gema Vizcay, Roland Fleck, Sarah J. Chapple, Giovanni E. Mann, Mark Fowler, Richard C. Siow
Summary: The activation of Nrf2 pathway is crucial for maintaining vascular endothelial redox homeostasis under high shear stress. This study reveals the important role of glycocalyx sialic acids (SIA) in regulating Nrf2 signaling in response to shear stress, demonstrating a synergistic relationship between SIA and Nrf2 in preventing atherosclerosis. The findings suggest that targeting the endothelial glycocalyx could be a potential therapeutic approach for cardiovascular disease and redox dysregulation associated with aging.
Article
Medicine, General & Internal
Sharanya Murlidharan, Avinash L. Sangle, Madhuri Engade, Ajay B. Kale
Summary: This study describes the clinical and biochemical profile of children with hepatitis A infection in Western India. The results show that most patients were below 10 years old and presented with symptoms such as fever, abdominal pain, dark-colored urine, vomiting, and anorexia. Icterus and hepatomegaly were common findings. Additionally, the highest frequency of cases occurred during the monsoon season and most patients had a lower socioeconomic status.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Andrea de Bartolomeis, Licia Vellucci, Mark C. Austin, Giuseppe De Simone, Annarita Barone
Summary: Schizophrenia is a neurodevelopmental disorder characterized by synaptic alterations and abnormal cortical-subcortical connections. Current treatment options mainly focus on dopamine D2 receptor occupancy, while glutamatergic abnormalities remain untargeted. In recent years, D-amino acids acting as NMDAR modulators have emerged as potential augmentation strategies for treatment-resistant schizophrenia (TRS), which affects around 30-40% of patients and is characterized by cognitive deficits and functional impairment. This systematic review explores the efficacy of D-amino acids and their impact on synaptic architecture, as well as compounds targeting D-amino acid oxidase and D-aspartate oxidase enzymes.
Article
Biochemistry & Molecular Biology
Malin Eh, Alexander Tobias Kaczmarek, Guenter Schwarz, Daniel Bender
Summary: Sulfite oxidase (SOX) is an enzyme that contains both molybdenum and heme, and is responsible for oxidizing sulfite to sulfate. It has been found that one functional subunit in SOX is sufficient for catalysis, and electrons can be transferred between different subunits.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Alexander Tobias Kaczmarek, Daniel Bender, Titus Gehling, Joshua Benedict Kohl, Hulya-Sevcan Daimagueler, Jose Angel Santamaria-Araujo, Max Christoph Liebau, Anne Koy, Sebahattin Cirak, Guenter Schwarz
Summary: Isolated sulfite oxidase deficiency (ISOD) is a rare metabolic disorder caused by mutations in the SUOX gene, leading to a loss of sulfite oxidase (SO) function and accumulation of harmful metabolites, resulting in severe neurodegenerative diseases. The correct insertion of heme cofactor and molybdenum cofactor into SO is crucial for its function, with deficiencies in this process contributing to the development of ISOD.
JOURNAL OF INHERITED METABOLIC DISEASE
(2022)
Article
Cell Biology
Albert L. Misko, Laura D. Weinstock, Sitara B. Sankar, Amanda Furness, Yulia Grishchuk, Levi B. Wood
Summary: MLIV patients have significantly increased blood cytokine levels, which are related to motor dysfunction. The interferon pro-inflammatory axis is found to be important in MLIV pathology.
Article
Pharmacology & Pharmacy
Julika Pristup, Elke Schaeffeler, Sita Arjune, Ute Hofmann, Jose Angel Santamaria-Araujo, Patrick Leuthold, Nele Friedrich, Matthias Nauck, Simon Mayr, Mathias Haag, Thomas Muerdter, Franz-Josef Marner, Mary Relling, William E. Evans, Guenter Schwarz, Matthias Schwab
Summary: This study identified a methyltransferase involved in urothione synthesis and revealed a novel link between molybdenum cofactor catabolism and drug metabolism. The study demonstrated that TPMT genotype and phenotype are associated with urothione excretion levels and validated these findings in a population-based study.
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Urology & Nephrology
Alexander Fuchs, Jennifer Dederichs, Sita Arjune, Polina Todorova, Fabian Woestmann, Philipp Antczak, Anja Illerhaus, Birgit Gathof, Franziska Grundmann, Roman-Ulrich Mueller, Thorsten Annecke
Summary: The dimensions and integrity of the endothelial surface layer (ESL) are impaired in patients with ADPKD. Increased capillary density may be a compensatory mechanism for vascular dysfunction to ensure sufficient tissue perfusion and oxygenation.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Medicine, General & Internal
Larina A. Karner, Sita Arjune, Polina Todorova, David Maintz, Franziska Grundmann, Thorsten Persigehl, Roman-Ulrich Mueller
Summary: Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic kidney disease. The glomerular filtration rate (GFR) is not a reliable indicator of disease progression until decline begins. Kidney volumetry is often used to assess disease severity, but additional imaging markers are needed. This study examined cyst fraction as a potential marker and found that it correlated with eGFR and showed higher predictive accuracy than total kidney volume. Future clinical trials are encouraged to explore the predictive value of cyst fraction in combination with other biomarkers.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Urology & Nephrology
Sita Arjune, Simon Oehm, Polina Todorova, Ron T. Gansevoort, Stephan J. L. Bakker, Florian Erger, Thomas Benzing, Volker Burst, Franziska Grundmann, Philipp Antczak, Roman-Ulrich Mueller
Summary: A new biomarker, copeptin levels, has been identified to predict the progression of ADPKD and aid in treatment decision-making for patients.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Urology & Nephrology
Polina Todorova, Sita Arjune, Claudia Hendrix, Simon Oehm, Johannes Schmidt, Denise Krauss, Katharina Burkert, Volker Rolf Burst, Thomas Benzing, Volker Boehm, Franziska Grundmann, Roman-Ulrich Mueller
Summary: This study provides a detailed analysis of factors associated with the degree of polyuria caused by tolvaptan and puts them into the context of quality of life. The findings will contribute to optimized patient counseling regarding this treatment option in ADPKD.
KIDNEY INTERNATIONAL REPORTS
(2023)
Article
Urology & Nephrology
Sita Arjune, Martin R. Spaeth, Simon Oehm, Polina Todorova, Stefan J. Schunk, Katharina Lettenmeier, Seung-Hun Chon, Malte P. Bartram, Philipp Antczak, Franziska Grundmann, Danilo Fliser, Roman-Ulrich Mueller
Summary: In this study, the researchers investigated whether DKK3 could improve outcome prediction in ADPKD. The results demonstrated that ADPKD patients had significantly higher uDKK3 levels, and uDKK3 correlated positively with the Mayo classification and kidney function. Although the effect was no longer significant in multivariate regression analysis adjusting for other factors, adding copeptin levels and the interaction term between copeptin and uDKK3 to the model improved the variance explanation of eGFR slope. Future studies are needed to confirm these findings, but uDKK3 appears to be an attractive candidate for improving outcome prediction in ADPKD.
CLINICAL KIDNEY JOURNAL
(2023)
Article
Urology & Nephrology
Sita Arjune, Franziska Grundmann, Polina Todorova, Claudia Hendrix, Roman Pfister, Henrik Ten Freyhaus, Roman-Ulrich Mueller
Summary: This study investigated the cardiac involvement in patients with ADPKD and compared them with a control group. The results showed a higher prevalence of left ventricular hypertrophy and mitral regurgitation in ADPKD patients, while the left ventricular ejection fraction was higher compared to the control group. Echocardiographic parameters were significantly associated with markers of disease progression.
Article
Cell Biology
Sadrija Cukoski, Christoph Heinrich Lindemann, Sita Arjune, Polina Todorova, Theresa Brecht, Adrian Kuhn, Simon Oehm, Sebastian Strubl, Ingrid Becker, Ulrike Kammerer, Jacob Alexander Torres, Franziska Meyer, Thomas Schomig, Nils Grosse Hokamp, Florian Siedek, Ingo Gottschalk, Thomas Benzing, Johannes Schmidt, Philipp Antczak, Thomas Weimbs, Franziska Grundmann, Roman-Ulrich Muller
Summary: Ketogenic dietary interventions show potential benefits in ADPKD patients by reducing body fat and liver volume, and improving kidney function. The interventions are feasible and safe.
CELL REPORTS MEDICINE
(2023)
Article
Urology & Nephrology
Piotr Woznicki, Florian Siedek, Maatje D. A. van Gastel, Daniel Pinto Dos Santos, Sita Arjune, Larina A. Karner, Franziska Meyer, Liliana Lourenco Caldeira, Thorsten Persigehl, Ron T. Gansevoort, Franziska Grundmann, Bettina Baessler, Roman-Ulrich Mueller
Summary: The deep learning model developed in this study enables accurate segmentation of kidneys and liver, providing an objective assessment of TKV and TLV. Importantly, this method has been validated with MRI scans from 40 different scanners, making it feasible for implementation in clinical routine care.
Meeting Abstract
Transplantation
Simon Oehm, Sita Arjune, Philipp Antczak, Polina Todorova, Franziska Grundmann, Roman-Ulrich Mueller
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Simon Oehm, Konstantin Steinke, Sita Arjune, Polina Todorova, Christoph Lindemann, Fabian Woestmann, Franziska Meyer, Florian Siedek, Thomas Weimbs, Franziska Grundmann, Roman-Ulrich Mueller
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)