Article
Biotechnology & Applied Microbiology
Anahita Shariat, Fatemeh Sefidkon
Summary: The successful induction of tetraploidy in Satureja khuzitanica led to an increase in morphological parameters, biomass, essential oil yield, and phenolic compounds, indicating the potential for increased secondary metabolites and drug effectiveness in the plant.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Tatiana E. Samatadze, Olga Yu Yurkevich, Firdaus M. Khazieva, Irina Basalaeva, Elena A. Konyaeva, Alla E. Burova, Svyatoslav A. Zoshchuk, Alexander Morozov, Alexandra Amosova, Olga Muravenko
Summary: Polemonium caeruleum is a valuable medicinal herb with high productivity and active ingredient content in tetraploid plants. The stability of chromosomes and specific characteristics of the plant are significant for raw material identification.
Article
Horticulture
Liya Ding, Rong Liu, Yike Gao, Jianhua Xiao, Yi Lv, Jing Zhou, Qixiang Zhang
Summary: Tetraploids are important for the development of Iris cultivars, but they have not been found in the interspecific hybrid Iris x norrisii. By treating seeds with colchicine, tetraploid plants were successfully induced in Iris x norrisii. These tetraploids showed increased viability and had larger leaves, stomata, and pollen grains compared to their diploid counterparts. Tetraploidization resulted in larger flowers, longer fruits, and slightly larger and significantly longer selfing seeds. The induced tetraploid Iris x norrisii has the potential to produce genetically diverse progenies in a short time, making it suitable for further research on polyploidization in Iris.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Yanfen Gao, Junchao Ma, Jiaqi Chen, Qian Xu, Yanxia Jia, Hongying Chen, Weiqi Li, Liang Lin
Summary: This study successfully established a novel method for establishing homogeneous tetraploid embryogenic cell lines in Magnolia officinalis, maximizing the production of solid polyploids through regenerants of single cell origin, and confirming the stability of ploidy level through flow cytometry and chromosome counting.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ke Zheng, Jian Wang, Xiao Zhang, Yali Hou, Wenxuan Dong
Summary: This study successfully induced polyploidy in hazelnut plants, enriching the germplasm resources and providing an important prerequisite for hazelnut breeding.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Horticulture
Fazilat Fakhrzad, Abolfazl Jowkar, Akhtar Shekafandeh, Maryam Jafarkhani Kermani, Ali Moghadam
Summary: This study investigated the effects of polyploidy induction on different characteristics of wallflower. The results showed that chromosome doubled plants exhibited changes in trichome number, pollen size, stomata size, and chloroplast number in stomata guard cells. The tetraploid induced wallflowers also showed improvements in growth habit, leaf size and color, flowering period, flower longevity, and anthocyanin and total soluble solids contents. UPLC-MS analysis revealed changes in phytohormone contents in the tetraploid induced plants. These findings demonstrate that polyploidization is a promising tool for breeding and introducing new genetic resources in the floriculture industry.
SCIENTIA HORTICULTURAE
(2023)
Article
Biotechnology & Applied Microbiology
Ruiquan Dong, Yi Pei, Lingyan Hong, Jiangli Nie, Yuhang Chen, Huili Yan, Yanjun Liu
Summary: By using artificial polyploid induction, new varieties of horticultural crops with better quality can be generated. In this study, mixed culture was used to induce polyploidy of black currant. The best condition for polyploid induction of black currant was found, and the ploidy of seedlings was verified. Additionally, the morphological differences and stomatal characteristics of tetraploid plants and diploid plants were compared, and differentially expressed genes were identified through transcriptome sequencing analysis. This study enriched the germplasm resources of black currant and provided a new way for black currant breeding.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Horticulture
Le Thi Diem, Truong Hoai Phong, Hoang Thanh Tung, Hoang Dac Khai, Truong Thi Lan Anh, Nguyen Thi Nhu Mai, Do Manh Cuong, Vu Quoc Luan, Tran Que, Hoang Thi Nhu Phuong, Bui Van The Vinh, Duong Tan Nhut
Summary: In this study, a protocol for polyploid induction of Ngoc Linh ginseng was developed using silver nanoparticles for disinfection and colchicine treatment for embryo induction. Tetraploid Ngoc Linh ginseng plants with larger stomatal size, higher stomatal chloroplast density, and better growth were successfully induced.
SCIENTIA HORTICULTURAE
(2022)
Article
Multidisciplinary Sciences
Shumin Gao, Yahong Sun, Yan Zhou
Summary: Inducing 2n pollen through colchicine is an important method for hybridizing and breeding modern tetraploid roses, resulting in higher viability compared to spontaneous 2n pollen. The external morphology, in vitro germination rate, and pollen tube length of induced and spontaneous 2n pollen showed no significant differences.
Article
Biotechnology & Applied Microbiology
Sakineh Jokari, Akhtar Shekafandeh, Abolfazl Jowkar
Summary: In this study, tetraploids were induced in two Citrus species using colchicine, resulting in plants with superior morphological traits and enhanced physiological characteristics. These tetraploid plants have the potential to be used as rootstocks and as parents for future crossing.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Agronomy
Masumi Yamagishi, Yutaka Jitsuyama, Yoichiro Hoshino
Summary: This study induced tetraploid L. leichtlinii plants in vitro and evaluated their characteristics in the field. The tetraploid plants exhibited earlier flowering, wider leaves, and larger tepals compared to diploid plants. They also showed higher tolerance to Botrytis disease and tended to have increased bulb yield.
Editorial Material
Biochemistry & Molecular Biology
Brian S. Leander
Summary: A new study uncovers an ancient group of small phagotrophic flagellates that supports ideas about early evolutionary history.
Article
Biochemistry & Molecular Biology
Ainoa Escrich, Diego Hidalgo, Mercedes Bonfill, Javier Palazon, Raul Sanchez-Munoz, Elisabeth Moyano
Summary: Novel approaches to optimize the production of plant specialized metabolites are crucial to reach maximum productivity of plant biofactories. Plant polyploidization frequently enhances protein synthesis and thereby increases the biosynthesis of specialized metabolites. By treating immature embryos of Taxus baccata with colchicine, polyploid cell lines were successfully induced, which showed higher taxane pathway gene expression and 6.2-fold higher taxane production compared to diploid cells. This finding is a step forward towards developing a sustainable production system for the anticancer drug paclitaxel.
Article
Agronomy
Zi-Ming Ren, Liu Cui, Cong Gao, Yun Wu, Qi Cui, Dong-Ze Li, Li-Qun Zheng, Yi-Ping Xia
Summary: Comprehensive morphological and correlation analyses were performed on scales from three lily cultivars to investigate the relationship between scale morphological characteristics, initial nutrient status, and bulblet regeneration capacities. The results showed significant morphological differences between middle scale (MS) and outer scale (OS), and scale width was positively correlated with the quantity and quality of regenerated bulblets. Starch and soluble sugars accounted for a large portion of scale dry weight, and a higher initial ratio of sucrose to starch promoted bulblet formation.
Article
Plant Sciences
Xiuyu Li, Zijie Zhang, Yunhui Ren, Yue Feng, Qi Guo, Li Dong, Yuhan Sun, Yun Li
Summary: In this study, tetraploid black locust plants were induced by treating hypocotyls from in vitro-grown seedlings with different concentrations of colchicine, providing a simpler and more efficient method for tetraploid induction. Tetraploid plants showed significant differences in morphological characteristics compared to diploid plants, with stomatal size and density serving as stable markers for rapid screening of tetraploids.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Agricultural Engineering
Zhang Jun-jie, Yang Yue-sheng, Lin Meng-fei, Li Shu-qi, Tang Yi, Chen Han-bin, Chen Xiao-yang
INDUSTRIAL CROPS AND PRODUCTS
(2017)
Article
Biology
Junjie Zhang, Mengfei Lin, Hanbin Chen, Qin Zhu, Vu Ngoc Linh, Xiaoyang Chen
ARCHIVES OF BIOLOGICAL SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Junjie Zhang, Shuqi Li, Mengfei Lin, Endian Yang, Xiaoyang Chen
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2018)
Article
Plant Sciences
Mengfei Lin, Ruihu Jia, Juncheng Li, Mengjie Zhang, Hanbin Chen, Deng Zhang, Junjie Zhang, Xiaoyang Chen
Article
Multidisciplinary Sciences
Junjie Zhang, Endian Yang, Qian He, Mengfei Lin, Wei Zhou, Ruiqi Pian, Xiaoyang Chen
Article
Biotechnology & Applied Microbiology
Honghui Shi, Endian Yang, Yun Li, Xiaoyang Chen, Junjie Zhang
Summary: The study demonstrated that solid-state fermentation induced by specific microorganisms can enhance the protein content, amino acid content, and antioxidant capacity of drumstick leaf flour, while reducing fat, fiber, and anti-nutritional factors. Fermentation also led to an increase in phenolics and flavonoids in the flour.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Junjie Zhang, Mengfei Lin, Hanbin Chen, Xiaoyang Chen
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
(2017)
