Article
Medicine, General & Internal
Emmanuel Bujold, Alexandre Fillion, Florence Roux-Dalvai, Marie Pier Scott-Boyer, Yves Giguere, Jean-Claude Forest, Clarisse Gotti, Genevieve Laforest, Paul Guerby, Arnaud Droit
Summary: This study demonstrated that maternal urine proteomic profiling can differentiate women with and without preeclampsia. Specific proteins like ceruloplasmin and serpin A7 were upregulated in maternal urine before the diagnosis of preeclampsia and fetal growth restriction, suggesting their potential role as biomarkers for early detection.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Viktoria Bitsadze, Sylvie Bouvier, Jamilya Khizroeva, Eva Cochery-Nouvellon, Eric Mercier, Antonia Perez-Martin, Alexander Makatsariya, Jean-Christophe Gris
Summary: This study found that ADAMTS13 activity, antibodies, and antigen levels in newly pregnant women with obstetric antiphospholipid syndrome (oAPS) are associated with the occurrence of pre-eclampsia (PEcl). These markers serve as significant risk factors for PEcl and its subgroups, exhibiting acceptable discrimination power in predicting clinical events. ADAMTS13 may help identify pregnant women with oAPS who do not require escalated treatment strategies to prevent PEcl.
THROMBOSIS RESEARCH
(2021)
Article
Immunology
Chiara Tersigni, Donatella Lucchetti, Rita Franco, Filomena Colella, Caterina Neri, Laura Crispino, Alessandro Sgambato, Antonio Lanzone, Giovanni Scambia, Manu Vatish, Nicoletta Di Simone
Summary: PE is a common pregnancy disorder characterized by hypertension and proteinuria, with aberrant expression of HLA-DR in STBEVs specifically associated with PE. Further studies are needed to define the role of abnormal placental HLA-DR expression in PE pathogenesis and evaluate the potential application of detecting circulating HLA-DR positive STBEVs in diagnosing and predicting PE in early and mid-pregnancy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Fang Guo, Bao Zhang, Hao Yang, Yixi Fu, Yaning Wang, Jianming Huang, Mi Cheng, Xiaobo Li, Zhuojian Shen, Li Li, Ping He, Andy Peng Xiang, Shuaiyu Wang, Hongbo Zhang
Summary: The study revealed that the transcriptomes of placentae from EOPE were significantly different from controls, while those from LOPE were not. Conversely, the transcriptomes of peripheral blood from LOPE showed more differences from controls compared to EOPE. Classical biomarkers of pre-eclampsia were specifically expressed in extravillous trophoblast and syncytiotrophoblast, only being upregulated in EOPE. New biomarkers for EOPE and LOPE, such as EBI3, IGF2, ORMDL3, GATA2, and KIR2DL4, were experimentally verified with patient samples.
CELL PROLIFERATION
(2021)
Article
Oncology
Natalia Almeida, Jimmy Rodriguez, Indira Pla Parada, Yasset Perez-Riverol, Nicole Woldmar, Yonghyo Kim, Henriett Oskolas, Lazaro Betancourt, Jeovanis Gil Valdes, K. Barbara Sahlin, Luciana Pizzatti, A. Marcell Szasz, Sarolta Karpati, Roger Appelqvist, Johan Malm, Gilberto B. Domont, Fabio C. S. Nogueira, Gyoergy Marko-Varga, Aniel Sanchez
Summary: The Wise MS Transfer (WiMT) methodology was developed for deep identification of blood proteins in undepleted plasma samples. It successfully identified over 1200 proteins related to key biological processes in melanoma progression, such as the PI3K-Akt signaling pathway. WiMT holds the potential for large-scale screening studies and the discovery of novel proteins with key melanoma disease functions.
Article
Medicine, General & Internal
Maren-Helene Langeland Degnes, Ane Cecilie Westerberg, Manuela Zucknick, Theresa L. Powell, Thomas Jansson, Tore Henriksen, Marie Cecilie Paasche Roland, Trond Melbye Michelsen
Summary: This study identified placenta-derived proteins in the maternal circulation and described their longitudinal patterns across gestation. Additionally, it identified placenta-derived proteins that could predict gestational age, serving as potential biomarkers for placental function.
Article
Biochemistry & Molecular Biology
Lesia O. O. Kurlak, Paula J. J. Scaife, Louise V. V. Briggs, Fiona Broughton Pipkin, David S. S. Gardner, Hiten D. D. Mistry
Summary: This study aimed to investigate the relationship between the concentrations of trace elements and angiogenic markers in women with pre-eclampsia and their fetuses. The results showed that pregnant women with pre-eclampsia had lower levels of selenium, zinc, and manganese, and higher levels of copper. Additionally, maternal plasma zinc concentration was positively correlated with plasma sFlt-1 concentration, suggesting an imbalance in antioxidant status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Claire Philippat, Matthieu Rolland, Sarah Lyon-Caen, Isabelle Pin, Amrit K. Sakhi, Azemira Sabaredzovic, Cathrine Thomsen, Remy Slama
Summary: The study found that collecting repeated urine samples can better assess exposure during pregnancy and infancy for non-persistent chemicals such as phthalates. However, there was relatively low correlation across trimesters and between maternal and infant samples, highlighting the importance of collecting biospecimens in the assumed sensitive time window.
ENVIRONMENTAL POLLUTION
(2021)
Review
Multidisciplinary Sciences
Marianna Danielli, Roisin C. Thomas, Clare L. Gillies, Jiamiao Hu, Kamlesh Khunti, Bee Kang Tan
Summary: This is the most comprehensive and up to date systematic review and meta-analysis on blood biomarkers for the early prediction of pre-eclampsia, highlighting the urgent need for further research to identify blood biomarkers that could predict the development of pre-eclampsia.
Review
Cell Biology
Megan V. C. Barnes, Paschalia Pantazi, Beth Holder
Summary: Extracellular vesicles (EVs) play a crucial role in pregnancy and hold great promise as diagnostic and translational tools for gestational diseases. However, the standardization and reliability of EV isolation and characterization methodologies need improvement, and further research on the functionality of EVs is necessary.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Medicine, General & Internal
Shane Sakamaki-Ching, Suzaynn Schick, Gabriela Grigorean, Jun Li, Prue Talbot
Summary: The study found that acute dermal exposure to THS can alter the human plasma proteome, initiate mechanisms of skin inflammatory disease, and elevate urinary biomarkers of oxidative harm.
Article
Medicine, General & Internal
Adam C. Swensen, Jingtang He, Alexander C. Fang, Yinyin Ye, Carrie D. Nicora, Tujin Shi, Alvin Y. Liu, Tara K. Sigdel, Minnie M. Sarwal, Wei-Jun Qian
Summary: Urine proteins can be used as biomarkers for diagnosing and monitoring diseases, and a comprehensive urine proteome database can aid in the discovery of potential biomarkers. This study identified a wide range of proteins from urine samples of different disease conditions, with many unique proteins that may represent disease-associated proteins. The data provides valuable information for future discovery and validation studies of urine protein biomarkers for various diseases.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hagen M. Gegner, Thomas Naake, Aurelien Dugourd, Torsten Mueller, Felix Czernilofsky, Georg Kliewer, Evelyn Jaeger, Barbara Helm, Nina Kunze-Rohrbach, Ursula Klingmueller, Carsten Hopf, Carsten Mueller-Tidow, Sascha Dietrich, Julio Saez-Rodriguez, Wolfgang Huber, Rudiger Hell, Gernot Poschet, Jeroen Krijgsveld
Summary: Metabolomic and proteomic analyses of human plasma and serum samples have the potential to advance our understanding of disease biology. However, pre-analytical factors such as temperature and time can impact the integrity of the samples and there is a need for standardized operating procedures to ensure reliable data.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Nutrition & Dietetics
Huifeng Shi, Yuanhui Jiang, Pengbo Yuan, Lian Chen, Xiaoli Gong, Yike Yang, Yuanyuan Wang, Hai Jiang, You Li, Mengxing Sun, Yangyu Zhao, Yuan Wei
Summary: This study revealed a non-linear association between vitamin E concentrations in the first trimester and gestational vitamin E change with pre-eclampsia. The findings showed that vitamin E concentrations below 7.3 mg/L and gestational decrease were associated with increased risk of pre-eclampsia.
FRONTIERS IN NUTRITION
(2022)
Article
Obstetrics & Gynecology
Hannah Blakey, Ruyue Sun, Long Xie, Rebecca Russell, Nadia Sarween, James Hodson, Beata Hargitai, Tamas Marton, Desley A. H. Neil, Edwin Wong, Neil S. Sheerin, Kate Bramham, Claire L. Harris, Ellen Knox, Mark Drayson, Graham Lipkin
Summary: This study aimed to compare complement markers in maternal and fetal circulation, and placental tissue, between women with pre-eclampsia (PE) and healthy pregnant controls. The results showed that women with PE had lower concentrations of properdin and C4, and higher Ba. Ba concentration in the cord blood was also significantly higher in PE cases. In addition, the staining of C4d in the syncytiotrophoblast membrane was increased in PE cases. These findings suggest that inhibition of complement activation could be a potential therapeutic target for PE.
PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH
(2023)
Article
Obstetrics & Gynecology
Albaro Jose Nieto-Calvache, Jose M. Palacios-Jaraquemada, Alejandra Hidalgo, Lina Maria Vergara-Galliadi, Rafael Cortes Charry, Lorgio Rudy Aguilera Daga, Raul Verastegui Goyzueta, Gabriel Osanan, Julio Fernandez, Fanny Corrales, Wilson Mereci, Vicente Yuen-Chon, Enrique Guevara, Luis Armando Zuniga, Giron, Francisco Eduardo Turcios, Hernan Munoz, Aquilino Mario Perez, Paulo Meade, Nicolas Basanta, Juan Pablo Pineda
Summary: Most reference centers in Latin America only handle a small number of PAS cases each year, and nearly all of them recognize areas for improvement in the management or approach for PAS in women.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Article
Engineering, Biomedical
Yadileiny Portilla, Vladimir Mulens-Arias, Alberto Paradela, Antonio Ramos-Fernandez, Sonia Perez-Yague, M. Puerto Morales, Domingo F. Barber
Summary: This study investigated the intracellular transit of two magnetic nanoparticles (MNPs) with different surface coatings in mouse cell lines. The results showed that the coating type influenced the intracellular trafficking rate and the nature of the endolysosomes in macrophages. The MNPs with a specific coating had a slower transit rate and resulted in endolysosomes with more lytic enzymes and catalytic proteins in macrophages. These MNPs also induced more autophagic vesicles and enhanced the expression of iron metabolism-related genes and proteins.
Article
Biochemistry & Molecular Biology
Laura Guerrero, Bruno Sangro, Veronica Ambao, Jose Ignacio Granero, Antonio Ramos-Fernandez, Alberto Paradela, Fernando J. Corrales
Summary: Precision medicine aims to tailor treatments based on molecular features of diseases, with proteomics providing a way to identify disease-associated proteins. Informative biomarkers are urgently needed for early diagnosis of liver disease, and the systematic quantification of enzymes related to cellular pathways may serve as a promising biomarker for patient stratification.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Nuria Colome, Joaquin Abian, Kerman Aloria, Jesus M. Arizmendi, Silvia Barcelo-Batllori, Sophie Braga-Lagache, Odile Burlet-Schiltz, Montse Carrascal, J. Ignacio Casal, Eduard Chicano-Galvez, Cristina Chiva, Luis Felipe Clemente, Felix Elortza, Josep M. Estanyol, Joaquin Fernandez-Irigoyen, Patricia Fernandez-Puente, Maria Jose Fidalgo, Carine Froment, Manuel Fuentes, Carlos Fuentes-Almagro, Marina Gay, Alexandre Hainard, Manfred Heller, Maria Luisa Hernandez, Nieves Ibarrola, Ibon Iloro, Thomas Kieselbach, Antonio Lario, Marie Locard-Paulet, Anabel Marina-Ramirez, Luna Martin, Esperanza Morato-Lopez, Javier Munoz, Rosana Navajas, M. Antonia Odena, Leticia Odriozola, Eliandre de Oliveira, Alberto Paradela, Carla Pasquarello, Vivian de los Rios, Cristina Ruiz-Romero, Eduard Sabido, Manuel Sanchez del Pino, Jaime Sancho, Enrique Santamaria, Christine Schaeffer-Reiss, Justine Schneider, Carolina de la Torre, M. Luz Valero, Marta Vilaseca, Shuai Wu, Linfeng Wu, Pilar Ximenez de Embun, Francesc Canals, Fernando J. Corrales
Summary: Global analysis of protein phosphorylation using mass spectrometry has become a powerful tool in biological and biomedical research, but it presents challenges such as low stoichiometry and the need for residue-specific information. The Proteomics Multicentric Experiment 11 (PME11) was launched to investigate the performance of phosphoprotein analysis protocols, with a reference sample distributed to 31 laboratories for evaluation. The results indicate the suitability of the PME11 reference sample for benchmarking and optimizing phosphoproteomics strategies while evaluating intra and inter laboratory performance.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Rosana Navajas, Antonio Ramos-Fernandez, Ignacio Herraiz, Alberto Galindo, Jose Luis Bartha, Fernando Corrales, Alberto Paradela
Summary: This study identified and validated differential exosomal proteins in liquid biopsy of pregnant women, opening up new possibilities for early detection of pre-eclampsia. In addition, the functional impact of the proteome composition of purified pre-eclamptic exosomes in target cells provides new information to better understand changes in embryo-maternal interactions and the pathogenesis of this diseas.
CLINICAL PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Fatima Milhano Santos, Joana Mesquita, Joao Paulo Castro-de-Sousa, Sergio Ciordia, Alberto Paradela, Candida Teixeira Tomaz
Summary: Oxidative stress refers to the imbalance between pro-oxidants and antioxidants, leading to various retinal disorders. While the human body has defense mechanisms to counteract autoxidation, it may not always be sufficient to prevent cellular damage. Modern lifestyles and environmental factors contribute to increased chemical exposure and stress induction, further exacerbating oxidative stress.
Article
Plant Sciences
Salvador Gonzalez-Gordo, Marta Rodriguez-Ruiz, Alberto Paradela, Antonio Ramos-Fernandez, Francisco J. Corpas, Jose M. Palma
Summary: The physiological process of fruit ripening is closely related to the role of mitochondrial proteome, especially in mitochondrial electron transport chain and tricarboxylic acid cycle. Proteomic analysis revealed the key genes and pathways involved in fruit ripening. Moreover, the synthesis of antioxidant ascorbate in the mitochondrial proteome also plays a crucial role in fruit ripening.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Estefania Nunez, Irene Orera, Lorena Carmona-Rodriguez, Jose Ramon Pano, Jesus Vazquez, Fernando J. Corrales
Summary: COVID-19 has had devastating consequences for the global economy and human health. Proteomics analysis has identified serum proteins that are associated with disease severity, providing a molecular basis for clinical stratification. The protein alterations normalize after 100 days of leaving the hospital in an independent cohort, supporting their reliability as hallmarks of COVID-19 progression and grading. These findings contribute to the optimization of disease assessment and follow-up for COVID-19 patients.
Article
Biochemistry & Molecular Biology
Pilar Martinez-Moya, Sebastian Campusano, Dionisia Sepulveda, Alberto Paradela, Jennifer Alcaino, Marcelo Baeza, Victor Cifuentes
Summary: Xanthophyllomyces dendrorhous, a yeast that produces astaxanthin and mycosporines, has been shown to have a survival strategy against stressful environments. The transcription factors SKN7, ROX1, and YAP6 have been found to play important roles in regulating the synthesis of these secondary metabolites. The proteome and transcriptome analysis revealed significant metabolic changes in the mutant strains, particularly in the glycolysis/gluconeogenesis, pentose phosphate pathway, and citrate cycle. Absence of ROX1 resulted in a significant decline in carotenoid production, while the absence of SKN7 and YAP6 led to an increase in mycosporine and sterol synthesis, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Laura Guerrero, Alberto Paradela, Fernando J. Corrales
Summary: Liver diseases, including liver cancer, have a high mortality rate and the incidence is increasing. Risk factors for liver diseases include alcohol consumption, obesity, diabetes, viral infection, and genetic determinants. The reprogramming of one-carbon metabolism in liver diseases and monitoring the enzymes involved may be useful for stratification of liver disease patients and precision medicine strategies.
Review
Cell Biology
Miguel A. Ortega, Oscar Fraile-Martinez, Cielo Garcia-Montero, Alberto Paradela, Maria Asuncion Sanchez-Gil, Sonia Rodriguez-Martin, Juan A. De Leon-Luis, Claude Pereda-Cerquella, Julia Bujan, Luis G. Guijarro, Melchor Alvarez-Mon, Natalio Garcia-Honduvilla
Summary: The human placenta plays multiple roles in pregnancy and has a significant impact on both maternal and fetal well-being. Extracellular vesicles (EVs) are crucial for the development and functioning of the placenta, as well as for communication between the mother and fetus. These EVs have been identified as potential biomarkers for various obstetric pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yadileiny Portilla, Vladimir Mulens-Arias, Neus Daviu, Alberto Paradela, Sonia Perez-Yague, Domingo F. Barber
Summary: When iron oxide nanoparticles (IONPs) come into contact with biological fluids, they form protein corona complexes that vary in composition based on the origin of the serum. These complexes have an impact on the interaction between IONPs and macrophages, ultimately affecting the immune system.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Alejandra Isabel Navarro Leon, Marta Munoz, Natalia Iglesias, Cristina Blanco-Vazquez, Ana Balseiro, Fatima Milhano Santos, Sergio Ciordia, Fernando J. Corrales, Tania Iglesias, Rosa Casais
Summary: The lack of sensitive diagnostic methods for detecting Mycobacterium avium subsp. paratuberculosis (Map) subclinical infections has hindered the control of paratuberculosis. In this study, the serum proteomic profiles of naturally infected cows with different pathological forms of paratuberculosis were analyzed, and potential biomarkers for improving diagnostic sensitivity were identified. These findings contribute to a better understanding of the mechanisms involved in paratuberculosis pathogenesis.
JOURNAL OF PROTEOME RESEARCH
(2023)