Systemic transcriptome comparison between early- And late-onset pre-eclampsia shows distinct pathology and novel biomarkers

Objectives Pre-eclampsia is a leading cause of morbidity and mortality during pregnancy. Although the two forms of this disorder, early- (EOPE) and late-onset of pre-eclampsia (LOPE) are different, the underlying pathology remains elusive. We aim to unravel the difference and to identify novel biomarkers for EOPE and LOPE. Materials and Methods A complete comparison of both placental and peripheral blood transcriptomes was performed to investigate the pathology of pre-eclampsia. Single-cell transcriptomics of the maternal-fetal interface were integrated to identify novel biomarkers for EOPE and LOPE which were further verified at protein or mRNA level in patients. Results We found that the transcriptomes of placentae from EOPE, but not LOPE, were significantly different from their respective controls. Conversely, the transcriptomes of peripheral blood from LOPE were more different from their controls than EOPE. Importantly, we identified that several classical biomarkers of pre-eclampsia were expressed specifically in extravillous trophoblast and syncytiotrophoblast and only upregulated in EOPE, suggesting they should not be applied to all pre-eclampsia patients in general. We further identified novel biomarkers for EOPE and LOPE from differentially expressed genes (DEGs) of placental and peripheral blood, respectively. The new biomarkers EBI3, IGF2, ORMDL3, GATA2 and KIR2DL4 were experimentally verified with patient blood samples. Conclusion Our data demonstrate distinct pathology of EOPE and LOPE, and uncover new biomarkers that can be applied in diagnosis for pre-eclampsia.

Automated summary

The study revealed that the transcriptomes of placentae from EOPE were significantly different from controls, while those from LOPE were not. Conversely, the transcriptomes of peripheral blood from LOPE showed more differences from controls compared to EOPE. Classical biomarkers of pre-eclampsia were specifically expressed in extravillous trophoblast and syncytiotrophoblast, only being upregulated in EOPE. New biomarkers for EOPE and LOPE, such as EBI3, IGF2, ORMDL3, GATA2, and KIR2DL4, were experimentally verified with patient samples.