Review
Biochemistry & Molecular Biology
Bruna Sabatke, Izadora Volpato Rossi, Abel Sana, Leticia Bassani Bonato, Marcel I. Ramirez
Summary: The study of host-pathogen interactions has significantly increased in recent decades, with extracellular vesicles (EVs) playing an important role in facilitating intercellular communication during the interaction. This review focuses on the dynamic roles of EV release and capture during host-pathogen interactions, critically analyzing methodologies for obtaining and analyzing EVs. The review emphasizes key points to consider in EV biogenesis and uptake studies.
MOLECULAR MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Luis A. Arteaga-Blanco, Dumith Chequer Bou-Habib
Summary: The nanosized EVs released by cells play a crucial role in intercellular communication, regulating cellular homeostasis and potentially spreading pathogen-derived molecules. Studying EVs shed by human macrophages can provide insights into modulating host immune responses and developing innovative therapeutic strategies against infectious diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jonas Henrique Costa, Jaqueline Moraes Bazioli, Luidy Darllan Barbosa, Pedro Luis Theodoro dos Santos Junior, Flavia C. G. Reis, Tabata Klimeck, Camila Manoel Crnkovic, Roberto G. S. Berlinck, Alessandra Sussulini, Marcio L. Rodrigues, Taicia Pacheco Fill
Summary: The study revealed a novel phytopathogenic role of P. digitatum EVs and tryptoquialanine A in causing damage to citrus fruits during infection, implying that this alkaloid is exported in EVs during plant infection.
Article
Biochemistry & Molecular Biology
Anastasiia Artuyants, Jiwon Hong, Priscila Dauros-Singorenko, Anthony Phillips, Augusto Simoes-Barbosa
Summary: Trichomonas vaginalis is a parasitic protozoan in the human urogenital tract causing a common sexually transmitted infection. A dysbiotic microbiome accompanies trichomoniasis, characterized by depletion of commensal bacteria like Lactobacillus gasseri and overgrowth of bacterial vaginosis-associated species including Gardnerella vaginalis. This study examines the extracellular vesicles (EVs) produced by L. gasseri and G. vaginalis, revealing their specific protein cargo and their modulatory effects on T. vaginalis pathogenicity and host-pathogen interactions.
MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Saigopalakrishna S. Yerneni, Sarah Werner, Juliana H. Azambuja, Nils Ludwig, Rory Eutsey, Surya D. Aggarwal, Peter C. Lucas, Nathanael Bailey, Theresa L. Whiteside, Phil G. Campbell, N. Luisa Hiller
Summary: pEVs produced by Streptococcus pneumoniae affect macrophages in different ways and may serve as a reservoir for the bacteria, leading to inflammatory responses and tissue damage in mammalian hosts.
Review
Cell Biology
Jeffrey S. Schorey, Yong Cheng, William R. McManus
Summary: Intracellular bacterial pathogens release bacterial components inside and outside host cells to promote their survival and replication. While both bacteria- and host-derived vesicles play a role in infection, research on these vesicles has been largely separate, despite their potential impact on bacterial pathogenesis and host immunity.
JOURNAL OF CELL SCIENCE
(2021)
Review
Microbiology
Jian Wang, Yi-Li Chen, Yong-Kang Li, Ding-Kang Chen, Jia-Fan He, Nan Yao
Summary: Sphingolipids are important signaling molecules in microbial pathogens' development and pathogenicity, regulating the balance between microbes and hosts. Bacteria and viruses can utilize host sphingolipids to promote infection, while sphingolipids are also involved in fungus-host interactions.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Gastroenterology & Hepatology
Xiao Liang, Nini Dai, Kangliang Sheng, Hengqian Lu, Jingmin Wang, Liping Chen, Yongzhong Wang
Summary: Intestinal microenvironment dysbiosis is a major cause of various diseases, and the use of gut bacteria-derived extracellular vesicles (GBEVs) shows potential in treatment. Understanding the role of GBEVs in bacteria-host communication is crucial for developing effective clinical strategies.
Review
Immunology
Chaoyu Zou, Yige Zhang, Huan Liu, Yu Wu, Xikun Zhou
Summary: Extracellular vesicles (EVs) are nanosized lipid particles released by living cells, which carry bioactive molecules and regulate cell growth and metabolism. They play significant roles in the interaction between host and bacteria, and have great application potential in various fields.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Shuaishuai Huang, Xin Zhao, Zhibing Luo, Xiaohan Tang, Yonghong Zhou, Nemat Keyhani, Yongjun Zhang, Chengshu Wang
Summary: In this study, transcriptome data from different stages of B. bassiana's life cycle were analyzed to identify gene modules enriched during key stages of infection. The results provide insights into the unique features of metabolism, signaling, and host defense suppression at different stages of the infection process.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Shuaishuai Huang, Xin Zhao, Zhibing Luo, Xiaohan Tang, Yonghong Zhou, Nemat Keyhani, Yongjun Zhang
Summary: This study analyzed global transcriptomic data of 76 samples to examine different life stages of Beauveria bassiana, a fungal insect pathogen. The analyses revealed unique gene modules involved in penetration of the host's cuticle and evasion of the host immune defenses. This comprehensive framework provides insights into fungal adaptations during its pathogenic life cycle and expands our understanding of host-pathogen interactions.
MICROBIOLOGY SPECTRUM
(2023)
Review
Microbiology
Krzysztof Grzymajlo
Summary: This review sheds light on the current understanding of the interactions between enteric pathogens, host gut microbiota, and the gastrointestinal tract. It discusses the strengths and limitations of in vitro and in vivo models used in studying Salmonella-host-microbiome interactions, and outlines future challenges and directions.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Parasitology
Ankur Midha, Friederike Ebner, Josephine Schlosser-Brandenburg, Sebastian Rausch, Susanne Hartmann
Summary: Ascariasis is a globally spread intestinal nematode infection that poses a significant threat to humans and pig husbandry. The complex body migration of Ascaris involves interactions with host cells and a microbial environment, highlighting the importance of research on this trilateral relationship. Understanding these interactions can lead to novel approaches to combat this widespread infection.
TRENDS IN PARASITOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ana Rita Cavaco, Ana Rita Matos, Andreia Figueiredo
Summary: Lipids and fatty acids play crucial roles in plant immunity, functioning as the first barriers to pathogen invasion and mediating interactions and immune responses between plants and pathogens. Studies suggest that lipids may serve as candidate biomarkers for resistance or susceptibility to pathogens, and they may also play a role in intercellular communication and systemic acquired resistance during plant-pathogen interactions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Parasitology
Xing He, Weiqing Pan
Summary: Understanding host-parasite interactions is crucial for controlling parasitic diseases, and miRNAs play a significant role in these interactions, with cross-species miRNA exchanges being common and important for host defense and parasite survival.
TRENDS IN PARASITOLOGY
(2022)
Article
Virology
Mauro M. Oliveira, Michelle O. Schemberger, Andreia A. Suzukawa, Irina N. Riediger, Maria do Carmo Debur, Guilherme Becker, Paola Cristina Resende, Tiago Graf, Eduardo Balsanelli, Valter Antonio de Baura, Emanuel M. de Souza, Fabio O. Pedrosa, Lysangela R. Alves, Lucas Blanes, Sheila Cristina Nardelli, Alessandra M. Aguiar, Letusa Albrecht, Dalila Zanette, Andrea R. Avila, Luis Gustavo Morello, Fabricio K. Marchini, Hellen G. dos Santos, Fabio Passetti, Bruno Dallagiovanna, Helisson Faoro
Summary: The genomic surveillance of SARS-CoV-2 lineages in Parana, southern Brazil, from March 2020 to April 2021 revealed the dominance of the Gamma strain, as well as high frequencies of the Gamma-like-II lineage and the S:E661D mutation. Further efforts in epidemiological and genomic surveillance are needed to understand the implications of these novel mutations identified in the VOC Gamma in Parana.
Article
Microbiology
Tamires A. Bitencourt, Otavio Hatanaka, Andre M. Pessoni, Mateus S. Freitas, Gabriel Trentin, Patrick Santos, Antonio Rossi, Nilce M. Martinez-Rossi, Lysangela L. Alves, Arturo Casadevall, Marcio L. Rodrigues, Fausto Almeida
Summary: In this study, the researchers investigated the role of extracellular vesicles (EVs) in fungal communication. They found that EVs from Candida albicans, Aspergillus fumigatus, and Paracoccidioides brasiliensis can regulate the expression of target genes and phenotypic features. The researchers propose that fungal EVs act as potent message effectors, and understanding their effects and mechanisms of action could be used in antifungal therapies.
Article
Microbiology
Isadora F. Munhoz da Rocha, Sharon T. Martins, Rafaela F. Amatuzzi, Daniel Zamith-Miranda, Joshua D. Nosanchuk, Marcio L. Rodrigues, Lysangela R. Alves
Summary: This study identified RNA content in extracellular vesicles (EVs) and cellular small RNA of Candida auris under normal growth conditions and after treatment with the antifungal drug caspofungin. The data set provides insights into fungal biology and may aid in understanding antifungal treatment, gene expression, and EV composition. This study contributes to the exploration of small RNA content in the fungal kingdom and sheds light on the mechanisms of molecules directed to fungal EVs.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Haroldo C. de Oliveira, Rafael F. Castelli, Lysangela R. Alves, Joshua D. Nosanchuk, Ehab A. Salama, Mohamed Seleem, Marcio L. Rodrigues
Summary: There is an urgent need to develop novel antifungals. This study identified four promising compounds with potential antifungal activities against various fungal pathogens.
Article
Microbiology
Leandro Honorato, Joana Feital Demetrio de Araujo, Cameron C. Ellis, Alicia Corbellini Piffer, Yan Pereira, Susana Frases, Glauber Ribeiro de Sousa Araujo, Bruno Pontes, Maria Tays Mendes, Marcos Dias Pereira, Allan J. Guimaraes, Natalia Martins da Silva, Gabriele Vargas, Luna Joffe, Maurizio Del Poeta, Joshua D. Nosanchuk, Daniel Zamith-Miranda, Flavia Coelho Garcia dos Reis, Haroldo Cesar de Oliveira, Marcio L. Rodrigues, Sharon de Toledo Martins, Lysangela Ronalte Alves, Igor C. Almeida, Leonardo Nimrichter
Summary: This study investigates the impact of fungal extracellular vesicles (EVs) on biofilm formation and virulence in Candida albicans. The researchers found that C. albicans EVs inhibit biofilm formation and promote pseudohyphae formation. EVs from different strains of C. albicans, S. cerevisiae, and H. capsulatum also inhibit yeast-to-hyphae differentiation. The inhibitory effect of C. albicans EVs is attributed to a combination of lipid compounds.
Article
Microbiology
Haroldo C. de Oliveira, Rafael F. Castelli, Flavia C. G. Reis, Kirandeep Samby, Joshua D. Nosanchuk, Lysangela R. Alves, Marcio L. Rodrigues
Summary: This study evaluated antifungal activities in a collection of 400 molecules and identified a non-toxic compound, MMV1593537, which controlled the growth of several lethal fungal pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Review
Cell Biology
Tamires A. Bitencourt, Andre M. Pessoni, Bianca T. M. Oliveira, Lysangela R. Alves, Fausto Almeida
Summary: The role of extracellular vesicles (EVs) in interkingdom and intraspecies communication, particularly the regulation of RNA content, plays a crucial role in the host-pathogen interactions and infection outcomes. Understanding the RNA profile and its effect on recipient cells in fungal EVs will lead to improved technologies to combat infectious agents threatening human health.
Article
Biochemistry & Molecular Biology
Anny Waloski Robert, Bruna Hilzendeger Marcon, Addeli Bez Batti Angulski, Sharon de Toledo Martins, Amanda Leitolis, Marco Augusto Stimamiglio, Alexandra Cristina Senegaglia, Alejandro Correa, Lysangela Ronalte Alves
Summary: This study investigates the effect of changing oxygen levels on the miRNA content of extracellular vesicles (EVs) derived from endothelial-like cells. The results show that the selective loading of miRNAs in the EVs is influenced by the oxygen status, and the targeted transcripts are involved in cell growth and survival pathways. These findings support the regenerative potential of EVs derived from endothelial-like cells and suggest that their properties can be fine-tuned by culture conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Immunology
Lysangela R. Alves, Alejandro Correa, Allan J. Guimaraes, Marcio L. Rodrigues
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Rafaela F. Amatuzzi, Daniel Zamith-Miranda, Isadora F. Munhoz da Rocha, Aline C. R. Lucena, Sharon de Toledo Martins, Rodrigo Streit, Charley C. Staats, Gabriel Trentin, Fausto Almeida, Marcio L. Rodrigues, Joshua D. Nosanchuk, Lysangela R. Alves
Summary: Antifungal resistance is increasing, with Candida auris being highly resistant to antifungal drugs. This study found that treatment with caspofungin led to increased production of extracellular vesicles (EVs) in C. auris and altered the content of RNA and proteins enclosed in the EVs. The ability of C. auris to change the composition of EVs may contribute to its ability to evade antifungal treatment.
Review
Biochemistry & Molecular Biology
Andre Bittencourt Lorusso, Joao Antonio Carrara, Carolina Deuttner Neumann Barroso, Felipe Francisco Tuon, Helisson Faoro
Summary: Antimicrobial resistance is a global public health crisis caused by the emergence of resistant organisms and different mechanisms of antibiotic resistance. Efflux pumps, particularly in Gram-negative bacteria like Pseudomonas aeruginosa, play a significant role in multidrug resistance and extensively drug-resistant bacteria. Understanding their function, structure, and regulation is essential in combating antibiotic resistance and developing new drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Microbiology
Camila Oliveira, Fabiola Barbieri Holetz, Lysangela Ronalte Alves, Andrea Rodrigues Avila
Summary: Chagas disease is caused by Trypanosoma cruzi and is a neglected tropical disease. The protozoan has developed various mechanisms to infect and survive in different hosts. Proteins play a crucial role in the parasite's morphological and metabolic changes, and surface proteins are essential for virulence. Understanding the factors involved in these processes improves our knowledge of parasite pathogenesis. Genome sequencing has allowed us to gain a deeper understanding of gene reprogramming and identify critical molecules for survival, particularly those involved in differentiation into infective forms. This review focuses on regulated proteins and the mechanisms of gene expression modulation.
Article
Biochemistry & Molecular Biology
Aline Castro Rodrigues Lucena, Mariana Galvao Ferrarini, Willian Klassen de Oliveira, Bruna Hilzendeger Marcon, Luis Gustavo Morello, Lysangela Ronalte Alves, Helisson Faoro
Summary: Klebsiella pneumoniae is a nosocomial pathogen that spreads multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. They release outer membrane vesicles (OMVs) containing virulence and resistance factors, which may be associated with pathogenicity, survival, stress response, and resistance dissemination.
Article
Microbiology
Tamires A. Bitencourt, Otavio Hatanaka, Andre M. Pessoni, Mateus S. Freitas, Gabriel Trentin, Patrick Santos, Antonio Rossi, Nilce M. Martinez-Rossi, Lysangela L. Alves, Arturo Casadevall, Marcio L. Rodrigues, Fausto Almeida
Summary: This study investigates the role of fungal extracellular vesicles (EVs) as potent message effectors in regulating gene expression and phenotypic features in pathogenic fungi.