Linker Engineering in the Context of Synthetic Protein Switches and Sensors

Allostery of multidomain proteins with disordered linkers

(2020) Qiaojing Huang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate

(2020) Yufeng Zhao et al.   ACS Central Science

Bright and High-Performance Genetically Encoded Ca2+ Indicator Based on mNeonGreen Fluorescent Protein

(2020) Landon Zarowny et al.   ACS Sensors

Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

(2020) Fangmiao Sun et al.   NATURE METHODS

Engineering Protein Switches for Rapid Diagnostic Tests

(2020) Hope Adamson et al.   ACS Sensors

A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP

(2019) Mark A. Lobas et al.   Nature Communications

A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine

(2019) Jiesi Feng et al.   NEURON

Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors

(2019) Hsien-Wei Yeh et al.   Annual Review of Analytical Chemistry

An efficient expression tag library based on self-assembling amphipathic peptides

(2019) Weixin Zhao et al.   Microbial Cell Factories

A genetically encoded fluorescent sensor for in vivo imaging of GABA

(2019) Jonathan S. Marvin et al.   NATURE METHODS

Linker Dependence of Avidity in Multivalent Interactions between Disordered Proteins

(2019) Charlotte S. Sørensen et al.   JOURNAL OF MOLECULAR BIOLOGY

Dynamic tuning of FRET in a green fluorescent protein biosensor

(2019) Pablo Trigo-Mourino et al.   Science Advances

Effective concentrations enforced by intrinsically disordered linkers are governed by polymer physics

(2019) Charlotte S. Sørensen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Minute-scale persistence of a GPCR conformation state triggered by non-cognate G protein interactions primes signaling

(2019) Tejas M. Gupte et al.   Nature Communications

Affimer–Enzyme–Inhibitor Switch Sensor for Rapid Wash-free Assays of Multimeric Proteins

(2019) Hope Adamson et al.   ACS Sensors

iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains

(2019) Alexander Gräwe et al.   NUCLEIC ACIDS RESEARCH

Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET)

(2019) Eric J. Aird et al.   ACS Sensors

Engineering artificial signalling functions with proteases

(2019) Alexander Gräwe et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genetically Encoded Glutamate Indicators with Altered Color and Topology

(2018) Jiahui Wu et al.   ACS Chemical Biology

Dual-Color Bioluminescent Sensor Proteins for Therapeutic Drug Monitoring of Antitumor Antibodies

(2018) Martijn van Rosmalen et al.   ANALYTICAL CHEMISTRY

Insights into the Aggregation Mechanism of PolyQ Proteins with Different Glutamine Repeat Lengths

(2018) Tetyana Yushchenko et al.   BIOPHYSICAL JOURNAL

A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578

(2018) Yi Shen et al.   BMC BIOLOGY

A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies

(2018) Miao Jing et al.   NATURE BIOTECHNOLOGY

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

(2018) Tommaso Patriarchi et al.   SCIENCE

Tunable molecular tension sensors reveal extension-based control of vinculin loading

(2018) Andrew S LaCroix et al.   eLife

Genetically Encoded Förster Resonance Energy Transfer-Based Biosensors Studied on the Single-Molecule Level

(2018) Henning Höfig et al.   ACS Sensors

Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS

(2018) William H. Zhang et al.   Nature Chemical Biology

Semisynthetic sensor proteins enable metabolic assays at the point of care

(2018) Qiuliyang Yu et al.   SCIENCE

NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics

(2018) Natalia V. Barykina et al.   Scientific Reports

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

(2018) Eric C. Greenwald et al.   CHEMICAL REVIEWS

Ratiometric Bioluminescent Sensor Proteins Based on Intramolecular Split Luciferase Complementation

(2018) Yan Ni et al.   ACS Sensors

Novel genetically encoded fluorescent probes enable real-time detection of potassium in vitro and in vivo

(2017) Helmut Bischof et al.   Nature Communications

Ultrasensitive Scaffold-Dependent Protease Sensors with Large Dynamic Range

(2017) Viktor Stein et al.   ACS Synthetic Biology

Engineering Pak1 Allosteric Switches

(2017) Onur Dagliyan et al.   ACS Synthetic Biology

Affimer proteins are versatile and renewable affinity reagents

(2017) Christian Tiede et al.   eLife

Development of an Optical Zn2+ Probe Based on a Single Fluorescent Protein

(2016) Yan Qin et al.   ACS Chemical Biology

Detection of Antibodies in Blood Plasma Using Bioluminescent Sensor Proteins and a Smartphone

(2016) Remco Arts et al.   ANALYTICAL CHEMISTRY

Influence of Glu/Arg, Asp/Arg, and Glu/Lys Salt Bridges on α -Helical Stability and Folding Kinetics

(2016) Heleen Meuzelaar et al.   BIOPHYSICAL JOURNAL

The Role of Protein Loops and Linkers in Conformational Dynamics and Allostery

(2016) Elena Papaleo et al.   CHEMICAL REVIEWS

A computationally engineered RAS rheostat reveals RAS–ERK signaling dynamics

(2016) John C Rose et al.   Nature Chemical Biology

A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid l-Lysine Analysis

(2016) Victoria Steffen et al.   SENSORS

Rapid construction of metabolite biosensors using domain-insertion profiling

(2016) Dana C. Nadler et al.   Nature Communications

Library-Aided Probing of Linker Determinants in Hybrid Photoreceptors

(2016) Robert Ohlendorf et al.   ACS Synthetic Biology

Rangefinder: A Semisynthetic FRET Sensor Design Algorithm

(2016) Joshua A. Mitchell et al.   ACS Sensors

A new design for a green calcium indicator with a smaller size and a reduced number of calcium-binding sites

(2016) Natalia V. Barykina et al.   Scientific Reports

Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics

(2016) Nordine Helassa et al.   Scientific Reports

Construction of a linker library with widely controllable flexibility for fusion protein design

(2015) Gang Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enzymatic protein switches built from paralogous input domains

(2015) Jennifer Tullman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Relating sequence encoded information to form and function of intrinsically disordered proteins

(2015) Rahul K Das et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Quantitative Assessments of the Distinct Contributions of Polypeptide Backbone Amides versus Side Chain Groups to Chain Expansion via Chemical Denaturation

(2015) Alex S. Holehouse et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A sensor for quantification of macromolecular crowding in living cells

(2015) Arnold J Boersma et al.   NATURE METHODS

Expression and purification of short hydrophobic elastin-like polypeptides with maltose-binding protein as a solubility tag

(2015) Laure Bataille et al.   PROTEIN EXPRESSION AND PURIFICATION

Synthetic protein switches: design principles and applications

(2015) Viktor Stein et al.   TRENDS IN BIOTECHNOLOGY

Modulating protein activity using tethered ligands with mutually exclusive binding sites

(2015) Alberto Schena et al.   Nature Communications

Design of protein switches based on an ensemble model of allostery

(2015) Jay H. Choi et al.   Nature Communications

Harnessing the Unique Structural Properties of Isolated α-Helices

(2014) Carter J. Swanson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

(2014) Rudolf Griss et al.   Nature Chemical Biology

Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes

(2014) Thomas Thestrup et al.   NATURE METHODS

Protease-based synthetic sensing and signal amplification

(2014) Viktor Stein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis of the ultrasensitive calcium indicator GCaMP6

(2014) JingJin Ding et al.   Science China-Life Sciences

Abscisic acid dynamics in roots detected with genetically encoded FRET sensors

(2014) Alexander M Jones et al.   eLife

Switchable Reporter Enzymes Based on Mutually Exclusive Domain Interactions Allow Antibody Detection Directly in Solution

(2013) Sambashiva Banala et al.   ACS Chemical Biology

Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells

(2013) Rabia U. Malik et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Polyproline-II Helix in Proteins: Structure and Function

(2013) Alexei A. Adzhubei et al.   JOURNAL OF MOLECULAR BIOLOGY

Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1

(2013) Ahmed Saied F. Belal et al.   Molecular BioSystems

An optimized fluorescent probe for visualizing glutamate neurotransmission

(2013) Jonathan S Marvin et al.   NATURE METHODS

Rational design of a ligand-controlled protein conformational switch

(2013) O. Dagliyan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins

(2013) M. Schlapschy et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Positively Charged Residues Are the Major Determinants of Ribosomal Velocity

(2013) Catherine A. Charneski et al.   PLOS BIOLOGY

Fusion protein linkers: Property, design and functionality

(2012) Xiaoying Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

(2012) J. Akerboom et al.   JOURNAL OF NEUROSCIENCE

Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments

(2011) Vladimir L Kolossov et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Backbone-Driven Collapse in Unfolded Protein Chains

(2011) Daniel P. Teufel et al.   JOURNAL OF MOLECULAR BIOLOGY

Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors

(2011) Ismael Alicea et al.   JOURNAL OF MOLECULAR BIOLOGY

Semisynthesis of Fluorescent Metabolite Sensors on Cell Surfaces

(2011) Matthias A. Brun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Systematic control of protein interaction using a modular ER/K  -helix linker

(2011) S. Sivaramakrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody Detection by Using a FRET-Based Protein Conformational Switch

(2010) Misha V. Golynskiy et al.   CHEMBIOCHEM

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

Engineered allosteric activation of kinases in living cells

(2010) Andrei V Karginov et al.   NATURE BIOTECHNOLOGY

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

(2010) Kazuki Horikawa et al.   NATURE METHODS

Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design

(2008) Jasper Akerboom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

(2008) S. A. Hires et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic charge interactions create surprising rigidity in the ER/K  -helical protein motif

(2008) S. Sivaramakrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Calcium Sensing by GCaMP2

(2008) Qi Wang et al.   STRUCTURE