Article
Chemistry, Multidisciplinary
Zhifei Chen, Hongyu Liu, Xiao Han, Ping Xu, Fei Tao
Summary: In this study, the authors successfully synthesized 1,3-propanediol (1,3-PDO) from glucose using a well-established safety strain Klebsiella oxytoca. Through a systematic metabolic engineering strategy, they were able to enhance the production of 1,3-PDO, achieving a flask fermentation titer of 6.2 g L-1. These findings have significant implications for future research on the efficient bioconversion of glucose to 1,3-PDO.
REACTION CHEMISTRY & ENGINEERING
(2023)
Article
Pharmacology & Pharmacy
Beata Weber-Dabrowska, Maciej Zaczek, Malgorzata Lobocka, Marzanna Lusiak-Szelachowska, Barbara Owczarek, Filip Orwat, Norbert Lodej, Aneta Skaradzinska, Lukasz Laczmanski, Dariusz Martynowski, Marta Kaszowska, Andrzej Gorski
Summary: In recent years, multidrug-resistant strains of Klebsiella pneumoniae have become a global concern and bacteriophage therapy may provide a potential solution. This study evaluated the properties and effectiveness of bacteriophages against Klebsiella strains, identifying promising candidates for therapeutic purposes. Additionally, historical phages were examined, including one with lytic activity that has been maintained for nearly 60 years.
Article
Environmental Sciences
Tao Zhang, Shuang-yan Xu, Hui Lin, Jian Yang, Zhuo-qun Zhao, Damia Barcelo, Hua-bao Zheng
Summary: In this study, a new tylosin-degrading strain, TYL-T1, was isolated and its degradation mechanism was revealed through transcriptome and metabolite analysis. The results demonstrate the potential of TYL-T1 in removing tylosin from the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Tufail Bashir, Rowan D. Brackston, Christopher Waite, Ioly Kotta-Loizou, Matthew R. Carey, Christoph Engl, Martin Buck, Jorg Schumacher
Summary: This study investigates the molecular origins of phenotypic heterogeneity in clonal bacterial batch cultures under extreme low-nitrogen stress. The researchers found high heterogeneity of nifHDK gene expression, which codes for the structural genes of nitrogenase, in the model diazotroph Klebsiella oxytoca. Through analysis and experiments, they identified the likely molecular origins of this heterogeneity and discovered the significant effect of the regulatory gene ginK on nifHDK variance. This understanding can inform biotechnology strategies and potentially have implications for nitrogen fixation in natural soil environments.
Article
Microbiology
Nagender Ledala, Mishika Malik, Karim Rezaul, Sara Paveglio, Anthony Provatas, Aaron Kiel, Melissa Caimano, Yanjiao Zhou, Jonathan Lindgren, Kristyna Krasulova, Peter Illes, Zdenek Dvorak, Sandhya Kortagere, Sabine Kienesberger, Amar Cosic, Lisa Poeltl, Ellen L. Zechner, Subho Ghosh, Sridhar Mani, Justin D. Radolf, Adam P. Matson
Summary: In this study, it was shown that bacterial indole acts as a multifunctional mitigator of pathogenicity by suppressing toxin production, enhancing conversion of tilimycin to tilivalline, and activating PXR.
Review
Microbiology
Nakul Neog, Upasana Phukan, Minakshi Puzari, Mohan Sharma, Pankaj Chetia
Summary: Klebsiella oxytoca is emerging as an important opportunistic pathogen causing hospital infections, especially posing a high risk for immunocompromised patients. It can lead to a variety of diseases, including colitis and infective endocarditis, in addition to common urinary and respiratory tract infections. The bacterium exhibits resistance to a wide range of antibiotics.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Christopher J. Waite, Anya Lindstrom Battle, Mark H. Bennett, Matthew R. Carey, Chun K. Hong, Ioly Kotta-Loizou, Martin Buck, Jorg Schumacher
Summary: Free-living nitrogen-fixing bacteria can improve growth yields of non-leguminous plants and address nutrient imbalances in crop production. Nitrogen fixation is a resource-costly adaptation regulated by signaling processes, and reinvestment of fixed nitrogen is essential for sustained nitrogenase activity. The study highlights the quantitative resource allocation required for successful nitrogen fixation in free-living diazotrophs.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Jing Yang, Haiyan Long, Ya Hu, Yu Feng, Alan McNally, Zhiyong Zong
Summary: Klebsiella oxytoca is a complex of nine species that can cause various infections and is increasingly becoming resistant to antibiotics, posing a potential threat to human health.
CLINICAL MICROBIOLOGY REVIEWS
(2022)
Article
Pediatrics
Michelle Bous, Malte Tielsch, Cihan Papan, Elisabeth Kaiser, Regine Weber, Joerg Ingo Baumbach, Soeren L. Becker, Michael Zemlin, Sybelle Goedicke-Fritz
Summary: The study developed a method for analyzing volatile organic compound (VOC) profiles of different bacterial species using MCC/IMS. The MCC/IMS analysis showed clear differentiation between Klebsiella pneumoniae (KP) and Klebsiella oxytoca (KO), as well as high sensitivity, specificity, and positive predictive values for identifying these bacteria. The results suggest that MCC/IMS may be a useful tool for in vitro diagnostics.
FRONTIERS IN PEDIATRICS
(2023)
Article
Microbiology
Haiyan Long, Ya Hu, Yu Feng, Zhiyong Zong
Summary: The Klebsiella oxytoca complex consists of nine closely related species that cause human infections, with K. oxytoca and Klebsiella michiganensis being more commonly associated with human infections and carrying more virulence genes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Panwana Khunnonkwao, Sirima S. Jantama, Kaemwich Jantama, Claire Joannis-Cassan, Patricia Taillandier
Summary: By optimizing enzymatic hydrolysis and fermentation conditions, high concentration and yield of 2,3-butanediol were successfully produced from cassava starch, while reducing production costs, achieving an economically feasible production goal.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Veterinary Sciences
Takeshi Tsuka, Hiroichi Ozaki, Daisuke Saito, Toshiyuki Murase, Yoshiharu Okamoto, Kazuo Azuma, Tomohiro Osaki, Norihiko Ito, Yusuke Murahata, Tomohiro Imagawa
Summary: CTX-M-2-producing Klebsiella oxytoca strains with high ESBL production rates may spread clonally within a farm, while the bla(CTX-M-2) gene in Klebsiella pneumoniae may disseminate among farms through different plasmids. Monitoring ESBL genes, including bla(CTX-M-2), among causative agents of bacterial mastitis in cows can help develop relevant treatments and control practices.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Jiaxue Zhao, Yanyan Xi, Jiangfeng Zhang, Yuefei Jin, Haiyan Yang, Guangcai Duan, Shuaiyin Chen, Jinzhao Long
Summary: This study investigated the prevalence and diversity of CRISPR/Cas systems in 175 Klebsiella oxytoca strains. It was found that 58.86% of the strains possessed at least one confirmed CRISPR locus, with the I-F system being the most prevalent. The IV-A3 system was randomly distributed and could be divided into two subgroups. Space origin analysis revealed significant matches between some spacer sequences and mobile genetic elements. The phage tail tape measure protein and conjunctive transfer system protein were identified as important targets of the I-F and IV-A3 systems, respectively. The predicted PAM sequences for the different systems were also identified.
MOLECULAR GENETICS AND GENOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Fengli Wu, Shucai Wang, Yanfeng Peng, Yufeng Guo, Qinhong Wang
Summary: A novel strain of V. natriegens was engineered, capable of highly productive pyruvate synthesis within a short period of time. The strain achieved a yield of 54.22 g/L pyruvate from glucose within 16 hours and demonstrated efficient conversion of sucrose or gluconate into pyruvate.
MICROBIAL CELL FACTORIES
(2023)
Article
Engineering, Environmental
Shanshan Lin, Yinghao Song, Yang Huo, Qi Wang, Xiangru Liu, Ya Gao, Wei Fan, Mingxin Huo
Summary: This study investigated the copper transport and trafficking mechanisms using the Cu resistant strain Klebsiella oxytoca CAV 1374, and found that the strain is a suitable biosorbent for copper removal. Different uptake mechanisms and pathways were identified under different copper concentrations, providing critical insights into copper-uptake mechanisms of microorganisms.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Microbiology
Xiaoyu Qiu, Weiwei Wang, Lige Zhang, Lihua Guo, Ping Xu, Hongzhi Tang
Summary: We isolated and characterized a new thermophilic PAH-degrading strain, Hydrogenibacillus sp. N12, which can utilize naphthalene as a sole carbon source and co-metabolize other PAHs. Metabolites of naphthalene degradation were identified, and two possible metabolic pathways were proposed based on the identified compounds and whole-genome sequencing data.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Fei Tao, Hongzhi Tang, Shuguang Zhang, Mengke Li, Ping Xu
Summary: A comprehensive Web server, PSS, was developed for designing water-soluble mutants of α-helical membrane proteins based on QTY design. The server was successfully evaluated with microbial transmembrane proteins and GPCRs, showing its convenience, efficiency, accuracy, and rationality. PSS is readily available worldwide as a Web-based tool for QTY-based protein engineering.
Article
Chemistry, Multidisciplinary
Yuchi Zhang, Dui Ma, Zhifei Chen, Asma Iqbal, Jinhai Hu, Ming Chen, Xuejiao Liu, Tsegaye Tadesse Tsega, Hira Fazal, Ping Xu, Fei Tao, Jiantao Zai, Xuefeng Qian
Summary: The study introduces a highly-efficient renewable electrochemical system that can fix atmospheric CO2 into biomass chemicals and selectively oxidize a broad range of chemicals. By utilizing Fe2CoSe4 electrocatalysts and KI as the oxidant, overpotential and energy consumption can be significantly reduced.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Shiting Guo, Xiaoxu Tan, Jieni Zhu, Chuanjuan Lu, Chunyu Yang, Qian Wang, Chao Gao, Ping Xu, Cuiqing Ma
Summary: This study presents an efficient method for the in vitro enzymatic cascade synthesis of L-serine from glycerol, an unavoidable byproduct of the biofuel industry. By optimizing the concentration process, a high yield of L-serine was achieved within a short time. Furthermore, the application of this enzymatic cascade can be expanded to other biochemical production from glycerol, indicating its potential in supporting the biofuel industry.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Xing Liu, Xiao Han, Yuan Peng, Chunlin Tan, Jing Wang, Hongsong Xue, Ping Xu, Fei Tao
Summary: In this study, a highly efficient whole-cell catalyst for l-DOPA production using Vibrio natriegens as the chassis was developed. Vibrio natriegens exhibited rapid protein expression and catalytic rate, leading to higher productivity in the early stage of biocatalysis. These findings highlight the remarkable potential of Vibrio natriegens as a whole-cell catalysis chassis for the production of valuable chemicals.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biophysics
Dan Xiao, Chunxia Hu, Xianzhi Xu, Chuanjuan Lue, Qian Wang, Wen Zhang, Chao Gao, Ping Xu, Xia Wang, Cuiqing Ma
Summary: A biosensor capable of detecting both D-lactate and L-lactate was developed in this study, using an allosteric transcription factor LldR and amplified luminescent proximity homogeneous assay technology. The optimized biosensor, BLac-6, showed high accuracy in lactate concentration detection and was tentatively applied in industrial fermentation.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Biotechnology & Applied Microbiology
Chunlin Tan, Ping Xu, Fei Tao
Summary: Global warming and climate instability have sparked interest in using renewable carbon resources for sustainable chemical production. Cyanobacteria, with their ability to directly use light and CO2 as energy and carbon sources, respectively, are ideal cellular factories for carbon-negative production of chemicals. However, adapting cyanobacterial technology to industry still faces challenges such as low productivity, poor tolerance, and difficulties in product harvesting, which may be addressed by synthetic biology.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yubo Wang, Meng Zhang, Lexin Li, Jihong Yi, Jiyu Liang, Shuning Wang, Ping Xu
Summary: This study engineered Escherichia coli to increase the production of L-5-MTHF by introducing an additional biosynthetic pathway. After optimization, a significant increase in the yield of L-5-MTHF was achieved. This paves the way for further improvement of L-5-MTHF biosynthesis in E. coli.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mengke Li, Yanze Wang, Fei Tao, Ping Xu, Shuguang Zhang
Summary: This study designed variants of antibodies using the QTY code and found that these variants showed significantly decreased aggregation propensity. Molecular dynamics simulations showed that the QTY code design maintained antigen-binding affinity and structural stability. The study suggests that the QTY code has significant potential in mitigating antibody aggregation.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Chemistry, Multidisciplinary
Zhifei Chen, Hongyu Liu, Xiao Han, Ping Xu, Fei Tao
Summary: In this study, the authors successfully synthesized 1,3-propanediol (1,3-PDO) from glucose using a well-established safety strain Klebsiella oxytoca. Through a systematic metabolic engineering strategy, they were able to enhance the production of 1,3-PDO, achieving a flask fermentation titer of 6.2 g L-1. These findings have significant implications for future research on the efficient bioconversion of glucose to 1,3-PDO.
REACTION CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Yipeng Zhang, Liting Ma, Weikang Sun, Dan Xiao, Chuanjuan Lu, Qian Wang, Ping Xu, Cuiqing Ma, Chao Gao
Summary: In this study, a mutant aldol oxidase was found to catalyze the oxidation reactions required for d-3,4-DHBA production. An in vitro enzymatic cascade composed of four enzymes was constructed for efficient production of d-3,4-DHBA from d-xylose.
Article
Chemistry, Multidisciplinary
Pengju Ren, Zijian Tan, Yingying Zhou, Hongzhi Tang, Ping Xu, Haifeng Liu, Leilei Zhu
Summary: This study reports the potential application of CO2 fixation as the C1 source for initiating cracking in light cycle oil. Through a one-pot enzymatic cascade reaction, 1-naphthol is converted into 2'-carboxybenzyl-pyruvic acid with high atom economy and overall yield at room temperature.
Article
Chemistry, Multidisciplinary
Chunlin Tan, Fei Tao, Ping Xu
Summary: This study developed a cyanobacterial cell factory that can directly synthesize PLA from CO2, resulting in a significant increase in PLA production compared to conventional methods. The PLA produced by this cell factory has a high molecular weight and shows promise for commercialization.
