Article
Public, Environmental & Occupational Health
Angela Chiereghin, Rocco Maurizio Zagari, Silvia Galli, Alessandra Moroni, Liliana Gabrielli, Simona Venturoli, Isabella Bon, Giada Rossini, Ilaria Maria Saracino, Matteo Pavoni, Silvia Lafratta, Alessandro Deni, Silvia Felici, Michele Borghi, Luca Guerra, Luigi Raumer, Vittorio Lodi, Pierluigi Viale, Luciano Attard, Tiziana Lazzarotto
Summary: This study evaluated the sensitivity and specificity of five different commercial serological assays for SARS-CoV-2 specific antibodies, finding that IgG serological assays are reliable for retrospective diagnosis, while IgM assays have low sensitivity and IgA assays have a substantial rate of indeterminate results.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Multidisciplinary Sciences
Allison N. Grossberg, Lilia A. Koza, Aurelie Ledreux, Chad Prusmack, Hari Krishnan Krishnamurthy, Vasanth Jayaraman, Ann-Charlotte Granholm, Daniel A. Linseman
Summary: This study presents results from a multiplex serology test for assessing COVID-19 antibody responses, showing distinct dynamics of IgG, IgM, and IgA responses in patients with different disease severity. It suggests that antibody responses to SARS-CoV-2 may serve as important biomarkers for assessing the risk of viral transmission.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Tomoya Tsuchida, Yuko Nitahara, Shotaro Suzuki, Yuko Komase, Katherine Candray, Yasutoshi Kido, Yu Nakagama, Yukitaka Yamasaki, Mitsuru Imamura, Kimito Kawahata, Hiroyuki Kunishima, Shigeki Fujitani, Masamichi Mineshita, Takahide Matsuda
Summary: Serological testing can help identify COVID-19 infections missed by NAAT, providing important insights for diagnosis and treatment.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Review
Biochemistry & Molecular Biology
Fanwu Gong, Hua-xing Wei, Qiangsheng Li, Liu Liu, Bofeng Li
Summary: The global COVID-19 pandemic has led to the development of various clinical diagnosis methods, including nucleic acid testing and serological screening. Due to the unsatisfactory sensitivity of nucleic acid detection, serological screening has become widely used. These serological diagnostic methods help improve clinical efficiency and control the spread of COVID-19.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Sophie Martin, Christopher Heslan, Gwenaele Jegou, Leif A. Eriksson, Matthieu Le Gallo, Vincent Thibault, Eric Chevet, Florence Godey, Tony Avril
Summary: The SARS-CoV-2 pandemic has prompted the scientific community to develop tools to combat the infection by studying the immunogenic potential of viral proteins S, E, and M. Patients with moderate to severe diseases exhibit elevated Ig responses against these proteins, with reduced Ig binding observed with the spike G614 variant compared to the D614 variant. This research represents a powerful tool to test humoral responses against evolving SARS-CoV-2 variants and vaccine effectiveness.
Article
Chemistry, Analytical
Lokman Liv
Summary: Amidst the global COVID-19 pandemic, developing rapid and accurate methods for diagnosing both symptomatic and asymptomatic cases is crucial. This article discusses an electrochemical immunoassay platform developed for detecting the SARS-CoV-2 spike antibody, which showed high specificity and sensitivity in saliva and oropharyngeal swab samples.
MICROCHEMICAL JOURNAL
(2021)
Review
Immunology
Ananda M. Rodilla, Sooyun Tavolacci, Jazz Cagan, Tanay Shah, Sandeep Mittan, Philip C. Mack, Fred R. Hirsch
Summary: Compared to the general population, lung cancer patients have a higher risk of severe COVID-19 and associated mortality. Despite being prioritized for vaccination, clinical trials did not include these patients, raising concerns about vaccine efficacy and immune response. Recent investigations have focused on the humoral responses of lung cancer patients to COVID-19 vaccination.
Article
Chemistry, Analytical
Atchara Lomae, Pattarachaya Preechakasedkit, Orakan Hanpanich, Tugba Ozer, Charles S. Henry, Atsushi Maruyama, Ekawat Pasomsub, Angsana Phuphuakrat, Sirirat Rengpipat, Tirayut Vilaivan, Orawon Chailapakul, Nipapan Ruecha, Nattaya Ngamrojanavanich
Summary: The COVID-19 pandemic has accelerated the development of portable diagnostic tools for detecting and controlling the spread of the disease. In this study, a smartphone-assisted Sensit Smart potentiostat was combined with a paper-based electrochemical sensor to detect SARS-CoV-2. The sensor utilized a disposable paper-based device with a modified working electrode that captures the target DNA. The developed sensor demonstrated high specificity and achieved accurate detection of SARS-CoV-2 in patient samples.
Article
Chemistry, Analytical
Luciana de Souza Freire, Camila Macena Ruzo, Barbara Batista Salgado, Ariamna Maria Dip Gandarilla, Yonny Romaguera-Barcelay, Ana P. M. Tavares, Maria Goreti Ferreira Sales, Isabelle Cordeiro, Jaila Dias Borges Lalwani, Robert Matos, Henrique Fonseca Filho, Spartaco Astolfi-Filho, Stefan Talu, Pritesh Lalwani, Walter Ricardo Brito
Summary: This study developed a biosensor-based ELISA immunoassay for monitoring SARS-CoV-2 antibodies in human serum samples. It employed a specific receptor and a secondary antibody to detect and quantify the antibody concentration using the chronoamperometric method.
Article
Biochemistry & Molecular Biology
Morteza Hosseini, Ebtesam Sobhanie, Foad Salehnia, Guobao Xu, Hodjattallah Rabbani, Mahsa Naghavi Sheikholeslami, Ali Firoozbakhtian, Niloufar Sadeghi, Mohammad Hossein Farajollah, Mohammad Reza Ganjali, Houman Vosough
Summary: Coronavirus disease (COVID-19) poses a global threat to public health, and rapid and accurate diagnosis of SARS-CoV-2 is crucial. We developed a sensitive and high-throughput electrochemiluminescence immunosensor based on antigen detection for early clinical diagnosis of COVID-19.
BIOELECTROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Wanwisa Deenin, Abdulhadee Yakoh, Umaporn Pimpitak, Ekawat Pasomsub, Sirirat Rengpipat, Gaston A. Crespo, Sudkate Chaiyo
Summary: In this study, a new highly sensitive and selective COVID-19 diagnostic device was developed by combining the principle of ATKs with electrochemical detection, allowing quantification using a smartphone. The device utilized a screen-printed electrode inside a lateral flow device to exploit the binding affinity between SARS-CoV-2 antigen and ACE2. Electrochemical assay signal intensity on smartphones increased proportionally to the concentration of SARS-CoV-2 antigen. The sensor demonstrated excellent performance in assessing and screening COVID-19 and can be used to accurately verify diagnostic data while remaining rapid, simple, and inexpensive.
BIOELECTROCHEMISTRY
(2023)
Article
Medical Laboratory Technology
Hannah Wang, Catherine A. Hogan, Michelle Verghese, Daniel Solis, Mamdouh Sibai, ChunHong Huang, Katharina Roltgen, Bryan A. Stevens, Fumiko Yamamoto, Malaya K. Sahoo, James Zehnder, Scott D. Boyd, Benjamin A. Pinsky
Summary: This study found that the concentration of nucleocapsid antigen in plasma is associated with the severity of COVID-19, particularly with ICU admission. Antigenemia showed comparable diagnostic performance to upper respiratory NAAT, aiding in triaging patients for optimized intensive care utilization.
CLINICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shimaa Eissa, Mohammed Zourob
Summary: The study presents an electrochemical immunosensor for the detection of SARS-CoV-2 virus antigen, which shows good sensitivity and selectivity by integrating sample collection and detection tools into a single platform. The biosensor offers a promising diagnostic tool for rapid detection of COVID-19 virus antigen in nasal samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
Davood Rostamzadeh, Sahar Mortezagholi, Maedeh Alinejad, Seyed Reza Jooya, Maryam Eskandarian, Alireza Metvaei, Sedigheh Vafaei, Hasan Aboulghasemi, Vahid Younesi, Mahdi Shabani
Summary: The study investigated the serological patterns of SARS-CoV-2 specific IgM and IgG in hospitalized, recovered COVID-19 patients, and prepandemic normal serum specimens. By combining PCR with IgM/IgG results, the sensitivity of COVID-19 diagnosis was improved.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Shalu Yadav, Mohd Abubakar Sadique, Pushpesh Ranjan, Raju Khan
Summary: In this study, a straightforward and label-free electrochemical immunosensing strategy based on a glassy carbon electrode modified with a biocompatible and conducting biopolymer functionalized molybdenum disulfide-reduced graphene oxide (CS-MoS2/rGO) nanohybrid is proposed to investigate the SARS-CoV-2 virus. The immunosensor employs recombinant SARS-CoV-2 Spike RBD protein (rSP) to specifically identify antibodies against the SARS-CoV-2 virus. The results demonstrate that the immunosensor is capable of highly sensitive and specific detection of SARS-CoV-2 antibodies and can differentiate between positive and negative samples accurately.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biotechnology & Applied Microbiology
Arpana Parihar, Vasundhara Pandita, Raju Khan
Summary: This article discusses the use of 3D-printed human organoids for drug screening, as well as their application in personalized medicine for COVID-19. This technology allows for faster and cost-effective drug screening, potentially saving more lives.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Physical
Arpana Parihar, Sayeda Samina Ahmed, Palak Sharma, Nishant Kumar Choudhary, Farjana Akter, Md Ackas Ali, Zannatul Ferdous Sonia, Raju Khan
Summary: The lesser side effects and proven therapeutic efficacy of plant bioactive compounds have made them a preferred choice in the treatment of COVID-19. In this study, molecular docking and molecular dynamics simulations were used to screen and investigate the potential of phytochemicals against the non-structural protein Nsp-15. The lead compounds, 6-Hydroxyluteolin and Luteolin, showed low toxicity, favorable pharmacokinetic properties, and strong inhibitory efficacy. These compounds have the potential to be therapeutically beneficial against SARS-CoV-2, although further in vitro and in vivo investigations are needed to validate this.
MOLECULAR SIMULATION
(2023)
Article
Nanoscience & Nanotechnology
Pushpesh Ranjan, Shalu Yadav, Mohd Abubakar Sadique, Raju Khan, Avanish Kumar Srivastava
Summary: In this study, an electrochemical immunosensor based on a ZrO2-rGO-IL nanocomposite was developed for the detection of CEA breast cancer biomarker. The sensor exhibited high sensitivity and selectivity, and was able to detect CEA in both a broad detection range and serum samples.
ACS APPLIED NANO MATERIALS
(2022)
Review
Materials Science, Biomaterials
Ayushi Singhal, Amrita Singh, Apoorva Shrivastava, Raju Khan
Summary: Epitope imprinting is a promising method for creating specialized recognition sites that resemble natural biorecognition elements. Epitope-imprinted materials have gained a lot of attention recently in various fields. Electrochemical based sensors are proven to be promising tool for the quick, real-time monitoring of biomarkers. This review explores the techniques, approaches, and strategies of epitope imprinting and highlights their applicability in electrochemical quantification of biomarkers for timely disease monitoring.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biotechnology & Applied Microbiology
Arpana Parihar, Shalu Yadav, Mohd Abubakar Sadique, Pushpesh Ranjan, Neeraj Kumar, Ayushi Singhal, Vedika Khare, Raju Khan, Sathish Natarajan, Avanish K. K. Srivastava
Summary: Microbial pathogens pose a threat to the world, but traditional laboratory-based diagnostics have limitations such as high cost, bulky instruments, and skilled personnel requirements. Biosensors offer potential for faster, cost-effective, and user-friendly detection. This review discusses design and fabrication of point-of-care testing devices, advances in electrochemical techniques, and integration with smartphone and Internet-of-Things systems. The integration of biosensors with IoT/IoMT can track infectious disease spread and help prevent social and economic losses.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Analytical
Neeraj Kumar, Shalu Yadav, Mohd Abubakar Sadique, Raju Khan
Summary: An innovative electrochemical biosensor based on graphene quantum dots has been developed for the primary diagnosis of CD44 antigen, a breast cancer biomarker. The biosensor exhibits simple, rapid, and highly sensitive detection capabilities.
Article
Chemistry, Multidisciplinary
A. Parihar, N. K. Choudhary, P. Sharma, R. Khan
Summary: Nanomaterials have been widely used in the fabrication of biosensors for cancerous and non-cancerous disease diagnostics. Carbon nanomaterials, with their excellent physical and chemical properties, have a broad range of applications in various fields. In this review, the focus is on the innovations in carbon nanomaterials for the preparation of aptamer-based electrochemical cancer diagnostics devices, which offer higher sensitivity, accuracy, selectivity, and detection speed compared to traditional methods.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Ayushi Singhal, Raju Khan
Summary: A molecularly imprinted polymer (MIP) based electrochemical sensor was developed for the analysis of Bisphenol-A (BPA). The sensor showed a linear detection range of 1 nM to 50 μM, with a low detection limit (LOD) of 0.19 nM and a limit of quantification (LOQ) of 0.64 nM. The fabricated electrode was found to be selective, sensitive, and reusable, and successfully used for the analysis of BPA in real samples.
Review
Nanoscience & Nanotechnology
Arpana Parihar, Raju Khan
Summary: Early detection of cancer-associated biomarkers and appropriate therapeutic intervention can help prevent higher cancer mortality rate. Electrochemical biosensors have gained significant interest for their high sensitivity, selectivity, and affordability in early cancer detection. In this study, an aptasensor was developed for the inexpensive and label-free detection of the epidermal growth factor receptor (EGFR) antigen, which is linked to breast cancer.
ACS APPLIED NANO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Arpana Parihar, Palak Sharma, Nishant Kumar Choudhary, Raju Khan, Ayush Gupta, Raj Kumar Sen, Harish Chandra Prasad, Mohammad Ashiq
Summary: The green synthesis of CdS and CdS/rGO nanocomposites using Lactobacillus bacteria is reported in this study. The nanocomposites show good electrochemical properties, photocatalytic, and antimicrobial activity, making them suitable for various applications such as biosensing, photocatalysis, and antimicrobial activity.
ACS APPLIED BIO MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Anju Maholiya, Pushpesh Ranjan, Raju Khan, S. Murali, Rakesh C. Nainwal, Puneet S. Chauhan, N. Sathish, J. P. Chaurasia, Avanish K. Srivastava
Summary: Carbon dots (CDs) are a novel carbon nanomaterial with unique properties, such as tunable photoluminescence (PL), high aqueous solubility, biocompatibility, and low toxicity. They have shown significant growth-enhancing effects on plants by augmenting photosynthesis and improving water absorption. CDs also possess antimicrobial and antioxidant properties, making them effective in relieving biotic and abiotic stresses. Furthermore, they can be used as sensors in agriculture to detect herbicides and pesticides.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Materials Science, Multidisciplinary
Mohd. Abubakar Sadique, Shalu Yadav, Pushpesh Ranjan, Raghuraj Singh Chouhan, Ivan Jerman, Ashok Kumar, Saurabh Saigal, Sagar Khadanga, Raju Khan, Avanish K. Srivastava
Summary: In this study, a bio-functionalized carbon nitride-reduced graphene oxide with chitosan nanocomposite was fabricated and applied in an immunosensor for SARS-CoV-2 antibody detection. The immunosensor exhibited a wide detection range and an ultra-low detection limit, and the results from real serum samples demonstrated its high accuracy for screening.
MATERIALS ADVANCES
(2023)
Review
Chemistry, Multidisciplinary
Monu Kumar Shukla, Arpana Parihar, Chandrabose Karthikeyan, Deepak Kumar, Raju Khan
Summary: This review discusses the potential of multifunctional graphene quantum dots (GQDs) as a platform for receptor targeting, drug delivery, and bioimaging in pancreatic cancer. GQDs have the ability to selectively target pancreatic cancer cells and serve as drug delivery vehicles for controlled and targeted release of therapeutics. Additionally, GQDs show promise as imaging agents for pancreatic cancer detection and monitoring.
Article
Materials Science, Biomaterials
Shalu Yadav, Mohd Abubakar Sadique, Pushpesh Ranjan, Raju Khan, N. Sathish, Avanish K. Srivastava
Summary: An electrochemical immunosensor based on MoS2-PDA nanocomposite was developed for highly sensitive detection of SARS-CoV-2 nucleocapsid protein. The sensor exhibited high sensitivity and consistency with conventional RT-PCR test results.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Chemistry, Physical
Ayushi Singhal, Shalu Yadav, Mohd Abubakar Sadique, Raju Khan, Ajeet Kumar Kaushik, N. Sathish, Avanish K. Srivastava
Summary: The development of efficient electrochemical sensors using molecularly imprinted polymers (MIPs) has gained attention as an alternative to bio-recognition elements. The challenge of stability and sensitivity in MIP-based sensing technology can be addressed by modifying MIPs with electro-active nano-systems such as MXene. This review highlights the use of MXene-modified MIP electrochemical sensing platforms to overcome the limitations of traditional MIPs and discusses the potential applications in point-of-care testing (POCT).
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)