Journal
MICROCHEMICAL JOURNAL
Volume 168, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.microc.2021.106445
Keywords
Immunosensor; SARS-CoV-2; COVID-19; Gold-cluster; Square wave voltammetry
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Amidst the global COVID-19 pandemic, developing rapid and accurate methods for diagnosing both symptomatic and asymptomatic cases is crucial. This article discusses an electrochemical immunoassay platform developed for detecting the SARS-CoV-2 spike antibody, which showed high specificity and sensitivity in saliva and oropharyngeal swab samples.
Amid the global threat caused by the coronavirus disease 2019 (COVID-19) pandemic, developing sufficiently rapid, accurate, sensitive and selective methods of diagnosing both symptomatic and asymptomatic cases is essential to alleviating and controlling the pandemic's effects. This article describes an electrochemical immunoassay platform developed to determine the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) spike antibody by using gold-clusters capped with cysteamine, glutaraldehyde, the spike protein of the SARSCoV-2 antigen and bovine serum albumin on a glassy carbon electrode. The electrochemical oxidation signal of the antigen-based immunosensor at 0.9 V was used to detect the SARS-CoV-2 spike antibody. When saliva and oropharyngeal swab samples were analysed, the recovery and relative standard deviation values were 96.97%- 101.99% and 4.99%-5.74%, respectively. The method's limit of detection relative to the SARS-CoV-2 spike antibody in synthetic media and in saliva or oropharyngeal swab samples was 0.01 ag/mL, while the immunosensor's linear response to the SARS-CoV-2 spike antibody varied from 0.1 to 1000 ag/mL. The cross-reactivity of the Middle East respiratory syndrome-coronavirus spike antigen was evaluated after being immobilised onto the functionalised gold-cluster based sensor, indicated that the good specifity of the produced immunosensor.
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