Article
Pharmacology & Pharmacy
Xinxin Guo, Mingjun Sun, Peiyan Yang, Xingchen Meng, Ran Liu
Summary: The mast cell is a crucial component in the tumor microenvironment and has a dual role in regulating cancers. It releases various molecules to modulate immune responses, communicates with other cells, and influences tumor development. Understanding the role and mechanisms of mast cells can lead to new approaches in cancer treatment.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Oncology
Takashi Kuroe, Reiko Watanabe, Motohiro Kojima, Ryo Morisue, Masato Sugano, Takeshi Kuwata, Hitoshi Masuda, Shota Kusuhara, Nobuaki Matsubara, Shioto Oda, Tetsuo Ushiku, Genichiro Ishii
Summary: The study identified two patterns of tumor necrosis in renal cell carcinoma: dirty necrosis and ghost necrosis. Dirty necrosis was significantly associated with unfavorable clinicopathological factors, with the TN area and number of necrotic foci correlated with tumor size. This suggests that dirty necrosis may serve as an unfavorable prognostic indicator in surgically resected RCC.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Oncology
Takashi Kuroe, Reiko Watanabe, Ryo Morisue, Saori Miyazaki, Motohiro Kojima, Shawhay Charles Murata, Tokiko Nakai, Tetsuro Taki, Shingo Sakashita, Naoya Sakamoto, Nobuaki Matsubara, Hitoshi Masuda, Tetsuo Ushiku, Genichiro Ishii
Summary: Dirty necrosis (DN) in renal cell carcinoma is characterized by abundant neutrophil infiltration and the production of neutrophil extracellular traps (NETs), indicating systemic inflammation.
Article
Oncology
Harrys Kishore Charles Jacob, John Lalith Charles Richard, Rossana Signorelli, Tyler Kashuv, Shweta Lavania, Utpreksha Vaish, Ranjitha Boopathy, Ashley Middleton, Melinda Minucci Boone, Ramakrishnan Sundaram, Vikas Dudeja, Ashok Kumar Saluja
Summary: The study revealed a novel mode of communication between CTCs and neutrophils, mediated by EVs, which modulate early transcriptome changes in neutrophils and promote cluster formation. This indicates a potential deeper remodulatory effect of EVs on companion cells in clusters.
Article
Immunology
Julia Bolik, Freia Krause, Marija Stevanovic, Monja Gandrass, Ilka Thomsen, Sarah-Sophie Schacht, Eva Rieser, Miryam Mueller, Neele Schumacher, Juergen Fritsch, Rielana Wichert, Eithan Galun, Juri Bergmann, Christian Roeder, Clemens Schafmayer, Jan-Hendrik Egberts, Christoph Becker-Pauly, Paul Saftig, Ralph Lucius, Wulf Schneider-Brachert, Roja Barikbin, Dieter Adam, Matthias Voss, Wolfgang Hitzl, Achim Krueger, Boris Strilic, Irit Sagi, Henning Walczak, Stefan Rose-John, Dirk Schmidt-Arras
Summary: Metastasis is the primary cause of death in cancer patients, with endothelial ADAM17 identified as a key driver in this process. Further research focused on targeting ADAM17 could lead to novel therapeutic approaches for preventing metastasis.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Review
Immunology
Richard Felix Kraus, Michael Andreas Gruber
Summary: Neutrophils, as part of the innate immune response, play a crucial role in defending against pathogens but can also mediate tissue damage in various diseases. Recent scientific advancements have expanded our understanding of neutrophils, highlighting their importance beyond immune defense to maintaining overall body integrity. This review delves into the role of neutrophils, their migration processes, immune properties, and behavior in cancer environments.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Christine Youn, Cristina Pontaza, Yu Wang, Dustin A. Dikeman, Daniel P. Joyce, Martin P. Alphonse, Meng-Jen Wu, Sabrina J. Nolan, Mohamed A. Anany, Michael Ahmadi, Jeremy Young, Aron Tocaj, Luis A. Garza, Harald Wajant, Lloyd S. Miller, Nathan K. Archer
Summary: Staphylococcus aureus is a major cause of skin infections and the emergence of antibiotic-resistant strains has created a need for alternative treatments. This study found that tumor necrosis factor (TNF) promotes protection against S. aureus in the skin, with TNFR1 promoting neutrophil recruitment and TNFR2 preventing systemic spread of bacteria. Treatment with a TNFR2 agonist showed therapeutic efficacy against S. aureus and Pseudomonas aeruginosa skin infections, suggesting the potential for targeting TNFR1 and TNFR2 for protection against bacterial skin infections.
Article
Cell Biology
Deisy Segura-Villalobos, Monica Lamas, Claudia Gonzalez-Espinosa
Summary: This study found that the localization of mast cells within cyclic hypoxia regions in tumors can modify their transcriptional profile and activation parameters. Cyclic hypoxia causes important phenotypic changes in mast cells.
Article
Nutrition & Dietetics
Mayara S. R. Lima, Catarina Goncalves, Mafalda D. Neto, Maria Helena Macedo, Jaluza L. C. de Queiroz, Valeria C. da Silva, Izael de S. Costa, Christina da S. Camillo, Pedro Paulo de A. Santos, Aldo A. M. Lima, Lorenzo Pastrana, Bruna L. L. Maciel, Ana Heloneida A. Morais
Summary: The study evaluated the anti-inflammatory effect of trypsin inhibitor isolated from tamarind seeds, showing potential benefits in reducing inflammatory markers and improving intestinal health in animal experiments.
Article
Oncology
Tiffany R. Emmons, Thejaswini Giridharan, Kelly L. Singel, Anm Nazmul H. Khan, Jason Ricciuti, Kaitlyn Howard, Stephanie L. Silva-Del Toro, Ivy L. Debreceni, Cathelijn E. M. Aarts, Mieke C. Brouwer, Sora Suzuki, Taco W. Kuijpers, Ilse Jongerius, Lee-Ann H. Allen, Viviana P. Ferreira, Anna Schubart, Holger Sellner, Jorg Eder, Steven M. Holland, Sanjay Ram, James A. Lederer, Kevin H. Eng, Kirsten B. Moysich, Kunle Odunsi, Michael B. Yaffe, Emese Zsiros, Brahm H. Segal
Summary: Research has shown that ascites fluid supernatants from ovarian cancer patients in the tumor microenvironment can prolong neutrophil lifespan, decrease neutrophil density, and induce nuclear hypersegmentation. Ascites fluid supernatants induce distinct neutrophil clusters and stimulate complement deposition and signaling in neutrophils, resulting in neutrophil suppressor function.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Medicine, Research & Experimental
Flavia Neto de Jesus, Pierre-Yves von der Weid
Summary: In a mouse model of arthritis overexpressing TNF-alpha, the contractile activity of popliteal lymphatic vessels was altered, which may be related to the response of eNOS and iNOS.
Article
Immunology
Xiaobin Fang, Yueyi Weng, Xiaochun Zheng
Summary: Chronic spontaneous urticaria (CSU), a mast cell-driven disease, significantly affects quality of life. This study identified key genetic factors associated with mast cell activation in CSU and investigated their roles in CSU pathogenesis. Genes involved in chemokine (C-C motif) ligand 2 (CCL2), cholesterol 25-hydroxylase (CH25H), and tumor necrosis factor (TNF) signaling pathways were found to play crucial roles in CSU pathogenesis, and targeting them may provide potential therapeutic options for CSU.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Daniel S. Simpson, Jiyi Pang, Ashley Weir, Isabella Y. Kong, Melanie Fritsch, Maryam Rashidi, James P. Cooney, Kathryn C. Davidson, Mary Speir, Tirta M. Djajawi, Sebastian Hughes, Liana Mackiewicz, Merle Dayton, Holly Anderton, Marcel Doerflinger, Yexuan Deng, Allan Shuai Huang, Stephanie A. Conos, Hazel Tye, Seong H. Chow, Arfatur Rahman, Raymond S. Norton, Thomas Naderer, Sandra E. Nicholson, Gaetan Burgio, Si Ming Man, Joanna R. Groom, Marco J. Herold, Edwin D. Hawkins, Kate E. Lawlor, Andreas Strasser, John Silke, Marc Pellegrini, Hamid Kashkar, Rebecca Feltham, James E. Vince
Summary: Cell death is crucial in pathogen infections. IFNg sensitizes macrophages to TLR-induced death by triggering macrophage-intrinsic death ligands and caspase-8 enzymatic activity. This process requires BAX and BAK, the mitochondrial apoptotic effectors.
Article
Biochemistry & Molecular Biology
Patricia P. Yee, Wei Li
Summary: Tumor necrosis is a common histological feature and poor prognostic predictor in various cancers. The mechanism underlying tumor necrosis may be a synergistic consequence of metabolic stress and inflammation, leading to oxidative stress-induced cell death. Recent studies suggest that immune cells may be involved in amplifying tumor necrosis.
Article
Multidisciplinary Sciences
Guillaume Voiriot, Karim Dorgham, Guillaume Bachelot, Anne Fajac, Laurence Morand-Joubert, Christophe Parizot, Grigorios Gerotziafas, Dominique Farabos, Germain Trugnan, Thibaut Eguether, Clarisse Blayau, Michel Djibre, Alexandre Elabbadi, Aude Gibelin, Vincent Labbe, Antoine Parrot, Matthieu Turpin, Jacques Cadranel, Guy Gorochov, Muriel Fartoukh, Antonin Lamaziere
Summary: In this study, the immune-inflammatory biomarker signature in bronchoalveolar and blood samples of critically ill COVID-19 patients was identified and found to be associated with a poor 28-day outcome.
SCIENTIFIC REPORTS
(2022)
Article
Cardiac & Cardiovascular Systems
Roland Immler, Wiebke Nadolni, Annika Bertsch, Vasilios Morikis, Ina Rohwedder, Sergi Masgrau-Alsina, Tobias Schroll, Anna Yevtushenko, Oliver Soehnlein, Markus Moser, Thomas Gudermann, Eytan R. Barnea, Markus Rehberg, Scott Simon, Susanna Zierler, Monika Pruenster, Markus Sperandio
Summary: This study reveals the critical role of the voltage-gated potassium channel K(V)1.3 in calcium signaling and neutrophil trafficking during acute inflammatory processes. The findings provide evidence for the involvement of K(V)1.3 in key functions of neutrophils and suggest new therapeutic approaches for treating inflammatory disorders.
CARDIOVASCULAR RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Piero Portincasa, Leonilde Bonfrate, Mirco Vacca, Maria De Angelis, Ilaria Farella, Elisa Lanza, Mohamad Khalil, David Q. -H. Wang, Markus Sperandio, Agostino Di Ciaula
Summary: The gut microbiota consists of trillions of commensal microorganisms including bacteria, fungi, and viruses, which coexist in symbiosis with the host and have profound effects on human health. Short chain fatty acids (SCFAs) are the most abundant bacterial metabolites in the human body and play an important role in metabolic, endocrine, and immune functions. Diet can influence the composition and activity of the gut microbiota, as well as SCFAs production and metabolic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Tarik Bozoglu, Seungmin Lee, Tilman Ziegler, Victoria Jurisch, Sanne Maas, Andrea Baehr, Rabea Hinkel, Amelie Hoenig, Anjana Hariharan, Christina Inyeop Kim, Simon Decker, Haider Sami, Tobias Koppara, Ruppert Oellinger, Oliver J. Muller, Derk Frank, Remco Megens, Peter Nelson, Christian Weber, Angelika Schnieke, Markus Sperandio, Gianluca Santamaria, Roland Rad, Alessandra Moretti, Karl-Ludwig Laugwitz, Oliver Soehnlein, Manfred Ogris, Christian Kupatt
Summary: The study demonstrates a method of retargeting adeno-associated viruses (AAVs) to endothelial cells by coating them with second-generation polyamidoamine dendrimers (G2) linked to endothelial-affine peptides (CNN). This approach improves gene transfer efficiency, potentially enabling applications in vascular and atherosclerosis models.
Article
Hematology
Qiuyue Ma, Roland Immler, Monika Pruenster, Markus Sellmayr, Chenyu Li, Albrecht von Brunn, Brigitte von Brunn, Rosina Ehmann, Roman Woelfel, Matteo Napoli, Qiubo Li, Paola Romagnani, Ralph Thomas Boettcher, Markus Sperandio, Hans-Joachim Anders, Stefanie Steiger
Summary: This study investigates the impact of uric acid on neutrophils and finds that hyperuricemia impairs neutrophil function. Partial reduction of uric acid levels using uricase can partially restore the neutrophil defects. The study reveals that uric acid affects neutrophil migration by regulating intracellular pH and cytoskeletal dynamics. These findings have important implications for understanding immunodeficiency in kidney disease and sterile inflammation.
Article
Reproductive Biology
Zhi Ma, Mirjana Kessler, Anca Chelariu-Raicu, Markus Sperandio, Sven Mahner, Udo Jeschke, Viktoria von Schoenfeldt
Summary: The expression of sLeX in the receptive endometrium is enhanced by IL-1 beta, facilitating trophoblast adhesion during embryo implantation. This study provides important insights into the molecular mechanisms of embryo implantation.
BIOLOGY OF REPRODUCTION
(2023)
Article
Immunology
Sergi Masgrau-Alsina, Lou Martha Wackerbarth, Dae-sik Lim, Markus Sperandio
Summary: This study identifies MST1 as a critical regulator of neutrophil homeostasis and mobilization from the bone marrow, shedding light on its complex role in regulating innate immunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Ina Rohwedder, Lou Martha Wackerbarth, Kristina Heinig, Annamaria Ballweg, Johannes Altstaetter, Myriam Ripphahn, Claudia Nussbaum, Melanie Salvermoser, Susanne Bierschenk, Tobias Straub, Matthias Gunzer, Marc Schmidt-Supprian, Thomas Kolben, Christian Schulz, Averil Ma, Barbara Walzog, Matthias Heinig, Markus Sperandio
Summary: Newborns, especially premature infants, are prone to developing neonatal sepsis due to differences in immune system requirements during intrauterine and extrauterine life. Through transcriptomic analysis, we have identified the molecular mechanisms of neutrophil maturation and functional adaptation in fetal ontogeny. Our findings reveal constitutive activation of the noncanonical NF-KB pathway and upregulation of A20 in fetal neutrophils, leading to appropriate adaptation of neutrophil function during intrauterine fetal life but potentially hampering immune responses in prematurely born infants.
Article
Immunology
Aikaterini Gatsiou, Simon Tual-Chalot, Matteo Napoli, Almudena Ortega-Gomez, Tommy Regen, Rachit Badolia, Valeriana Cesarini, Claudia Garcia-Gonzalez, Raphael Chevre, Giorgia Ciliberti, Carlos Silvestre-Roig, Maurizio Martini, Jedrzej Hoffmann, Rana Hamouche, Joseph R. Visker, Nikolaos Diakos, Astrid Wietelmann, Domenico Alessandro Silvestris, Georgio Georgiopoulos, Ali Moshfegh, Andre Schneider, Wei Chan, Stefan Guenther, Johanne Backs, Shin Kwak, Craig H. Selzman, Kimon Stamatelopoulos, Stefan Rose-John, Christian Trautwein, Ioakim Spyridopoulos, Thomas Braun, Ari Waisman, Angela Gallo, Stavros G. Drakos, Stefanie Dimmeler, Markus Sperandio, Oliver Soehnlein, Konstantinos Stellos
Summary: RNA editor ADAR2 regulates endothelial responses to IL-6, controlling leukocyte trafficking in inflamed and ischemic tissues. ADAR2 is required for IL-6 trans-signaling and immune cell infiltration through suppressing primary microRNA processing.
Review
Medicine, General & Internal
Sebastian Sitaru, Agnes Budke, Riccardo Bertini, Markus Sperandio
Summary: Mounting experimental evidence suggests that the CXCL8-CXCR1/2 axis plays an essential role in neutrophils in the pathophysiology of inflammatory, autoimmune, and neoplastic diseases. Therapeutic targeting of CXCR1/2 or CXCL8 has been extensively studied using in vitro and animal disease models, showing potential benefits for patients with unwanted neutrophil-mediated inflammation. However, careful evaluation is needed for the use of inhibitors in severe infections or sepsis. Inhibition of the CXCL8-CXCR1/2 axis also holds promise for cancer therapy, with ongoing efforts to define its involvement in neoplastic diseases.
INTERNAL AND EMERGENCY MEDICINE
(2023)
Article
Allergy
Selina K. Jorch, Annika McNally, Philipp Berger, Jonas Wolf, Kim Kaiser, Andrian Chetrusca Covash, Stefanie Robeck, Isabell Pastau, Olesja Fehler, Saskia-L. Jauch-Speer, Sven Hermann, Michael Schafers, Hanne Van Gorp, Apurva Kanneganti, Joke Dehoorne, Filomeen Haerynck, Federica Penco, Marco Gattorno, Jae Jin Chae, Paul Kubes, Mohamed Lamkanfi, Andy Wullaert, Markus Sperandio, Thomas Vogl, Johannes Roth, Judith Austermann
Summary: This study provides evidence that S100A8/A9 alarmins are released through a pyrin/caspase-1/gasdermin D-dependent pathway in FMF patients, and they directly drive autoimmune inflammation in vivo.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Review
Cell Biology
Stefanie Schreiber, Jose Bernal, Philipp Arndt, Frank Schreiber, Patrick Mueller, Lorena Morton, Ruediger Christian Braun-Dullaeus, Maria Del Carmen Valdes-Hernandez, Roberto Duarte, Joanna Marguerite Wardlaw, Sven Guenther Meuth, Grazia Mietzner, Stefan Vielhaber, Ildiko Rita Dunay, Alexander Dityatev, Solveig Jandke, Hendrik Mattern
Summary: Brain vascular health is crucial in preventing and slowing down the progression of amyotrophic lateral sclerosis (ALS). Impaired brain vascular health in ALS leads to decreased levels of vascular endothelial growth factor, compromised endothelial cell formation, and blood-brain barrier integrity. This review discusses the pathogenesis of impaired brain vascular health in ALS and the potential of novel magnetic resonance imaging techniques in its detection. It also highlights the importance of blood supply patterns to the motor cortex as a marker of resistance and resilience against vascular risk and events in ALS, providing insights for ALS management and understanding of the disease.
Article
Immunology
Monika Pruenster, Roland Immler, Jonas Roth, Tim Kuchler, Thomas Bromberger, Matteo Napoli, Katrin Nussbaumer, Ina Rohwedder, Lou Martha Wackerbarth, Chiara Piantoni, Konstantin Hennis, Diana Fink, Sebastian Kallabis, Tobias Schroll, Sergi Masgrau-Alsina, Agnes Budke, Wang Liu, Dietmar Vestweber, Christian Wahl-Schott, Johannes Roth, Felix Meissner, Markus Moser, Thomas Vogl, Veit Hornung, Petr Broz, Markus Sperandio
Summary: This study elucidates the mechanisms of S100A8/S100A9 release from neutrophils, revealing that E-selectin regulates the release of S100A8/S100A9 through NLRP3 and gasdermin D. This process serves as a rapid and reversible activation system that plays a crucial role in inflammation.
Article
Multidisciplinary Sciences
Won Ho Yang, Peter V. Aziz, Douglas M. Heithoff, Yeolhoe Kim, Jeong Yeon Ko, Jin Won Cho, Michael J. Mahan, Markus Sperandio, Jamey D. Marth
Summary: The colonic mucosal barrier provides protection against infection, inflammation, and tissue ulceration. The erosion of this barrier is a common feature of colitis, but the underlying molecular mechanisms are not well understood. This study used a food poisoning model to investigate the erosion of the mucosal barrier. The findings suggest that previous infection activates an innate immune mechanism that leads to the activation of Neu3 neuraminidase in colonic epithelial cells and increased secretion of Cathepsin-G protease by Paneth cells. These host responses, combined with the desialylation of Mucin-2, render the mucosal barrier susceptible to proteolytic breakdown. Inhibiting Cathepsin-G or Neu3 function can protect against proteolysis and barrier erosion, reducing the frequency and severity of colitis.
