Article
Anesthesiology
Noemie Mermet-Joret, Cristina Alba-Delgado, Amelie Descheemaeker, Francois Gabrielli, Radhouane Dallel, Myriam Antri
Summary: Pain processing is immature in young mammals. The development of neurons in the medullary dorsal horn (MDH) has been overlooked. In this study, we investigate the age-dependent development of neurons in the inner lamina II (IIi) of the MDH in rats. We found that these neurons undergo physiological and structural changes, which are associated with facial mechanical allodynia.
Article
Neurosciences
In-Sun Choi, Jin-Hwa Cho, Michiko Nakamura, Il-Sung Jang
Summary: Menthol, acting as an agonist for TRPM8, has complex effects on nociceptive transmission, including increasing sEPSC frequency and sIPSC frequency. The increase in sEPSC frequency is mediated by TRPM8 and not TRPA1, involving Ca2+ permeable TRPM8 on primary afferent terminals.
Article
Neurosciences
Jing Zhang, Jialin Si, Rongrong Liang, Yuxin Lu, Hongwei Shang, Xinwei Li, Shukai Sun, Li-an Wu
Summary: This study showed that the severity of inflammation in dental pulp tissue increased over time in a rat dental pulpitis model. The expression of P2X7, HIF-1 alpha, IL-1 beta, and IL-18 in the medullary dorsal horn (MDH) peaked on the seventh day, when the pain threshold was the lowest. Furthermore, lipopolysaccharides (LPS) increased P2X7-mediated HIF-1 alpha expression in microglia. The suppression of P2X7 led to less release of IL-1 beta and IL-18, as well as lower HIF-1 alpha expression. Additionally, the P2X7 antagonist Brilliant Blue G (BBG) was found to alleviate pain behaviors in the dental pulpitis rats.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Kun-Long Zhang, Shu-Jiao Li, Xue-Yin Pu, Fei-Fei Wu, Hui Liu, Rui-Qing Wang, Bo-Zhi Liu, Ze Li, Kai-Feng Li, Nian-Song Qian, Yan-Ling Yang, Hua Yuan, Ya-Yun Wang
Summary: Mitochondria play a significant role in both inflammatory and neuropathic pain. Dynamin-related protein 1 (Drp1) is involved in the induction of mitochondrial fission and is associated with pain behavior. Overexpression of Drp1 attenuates pain hypersensitivity while down-regulation of Drp1 does not affect painful behavior.
Article
Neurosciences
Pavel Adamek, Mario Heles, Anirban Bhattacharyya, Monica Pontearso, Jakub Slepicka, Jiri Palecek
Summary: The novel FDA-approved PI3K delta/gamma isoform-specific inhibitor duvelisib shows promising ability in preventing paclitaxel-induced peripheral neuropathy, indicating its potential for use in human medicine.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Neurosciences
Candler Paige, Isabel Plasencia-Fernandez, Moeno Kume, Melina Papalampropoulou-Tsiridou, Louis-Etienne Lorenzo, Eric T. David, Lucy He, Galo L. Mejia, Christopher Driskill, Francesco Ferrini, Andrew L. Feldhaus, Leon F. Garcia-Martinez, Armen N. Akopian, Yves De Koninck, Gregory Dussor, Theodore J. Price
Summary: The findings suggest that CGRP promotes pain plasticity in female rodents but has a limited impact in males.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Racheli Wercberger, Joao M. Braz, Jarret A. Weinrich, Allan I. Basbaum
Summary: Using retro-TRAP and RNA sequencing, researchers have uncovered extensive molecular diversity of spino-and trigeminoparabrachial projection neurons, including distinct subsets of gene expression. Further investigation revealed significant functional heterogeneity in these projection neurons, showing convergence and segregation of pain-and itch-provoking inputs into molecularly diverse subsets of NK1R- and non-NK1R-expressing neurons.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Cristina Rodriguez, Anderson Chen, Jose A. Rivera, Manuel A. Mohr, Yajie Liang, Ryan G. Natan, Wenzhi Sun, Daniel E. Milkie, Thomas G. Bifano, Xiaoke Chen, Na Ji
Summary: The development of a compact adaptive optics module has enabled correction of tissue-induced aberrations in two-photon and three-photon fluorescence microscopes, allowing high-resolution imaging of synaptic structures in deep regions of the mouse brain and spinal cord, as well as somatosensory-evoked calcium responses. This advancement has facilitated structural and functional imaging deep within living organisms such as mice and zebrafish larvae.
Article
Pharmacology & Pharmacy
Zhifeng Huang, Zijing Ding, Yangting Xu, Caiyun Xi, Liqiong He, Hui Luo, Qulian Guo, Changsheng Huang
Summary: Regulation of gene transcription in the spinal dorsal horn (SDH) through the transcription factor STAT2 plays a crucial role in the development of neuropathic pain. Chronic constriction injury of the rat sciatic nerve led to the downregulation of nuclear STAT2 in the SDH, which was associated with increased microglial activation and the development of neuropathic pain. In addition, siRNA-induced downregulation of STAT2 promoted microglial activation and pro-inflammatory cytokine expression in the SDH. Restoring nuclear expression of STAT2 could potentially be a therapeutic pathway for treating neuropathic pain.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Fumihiro Saika, Shinsuke Matsuzaki, Shiroh Kishioka, Norikazu Kiguchi
Summary: The study focuses on investigating the sex-dependent roles of microglia in pain hypersensitivity, highlighting the importance of understanding the molecular aspects of spinal microglia regulation in pain. The results showed that chemogenetic activation of spinal microglia resulted in pain hypersensitivity in male mice, with sex differences observed in the upregulation of reactive microglia-dominant molecules and response to microglia depletion using pexidartinib.
Article
Neurosciences
Ghanshyam P. Sinha, Pranav Prasoon, Bret N. Smith, Bradley K. Taylor
Summary: The firing patterns of Neuropeptide Y Y1 receptor-expressing neurons in the spinal cord dorsal horn play a crucial role in chronic pain, predominantly controlled by T-type calcium channels. These neurons exhibit rapid adaptation, rebound spiking, and delayed firing, with an adaptation speed higher than other neurons.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Medicine, Research & Experimental
Tyler S. Nelson, Heather N. Allen, Paramita Basu, Pranav Prasoon, Eileen Nguyen, Cynthia M. Arokiaraj, Diogo F. S. Santos, Rebecca P. Seal, Sarah E. Ross, Andrew J. Todd, Bradley K. Taylor
Summary: Neuropeptide Y targets the Y1 receptor to produce analgesia in the spinal dorsal horn. The Y1-INs, which express the Y1 receptor, are necessary and sufficient for neuropathic hypersensitivity. This study identified three subpopulations of Y1-INs based on their coexpression with gastrin releasing peptide, neuropeptide FF, and cholecystokinin. Among these subpopulations, the Grp/Npy1r-INs were found to be conserved in higher order mammalian species and may represent a promising target for the treatment of neuropathic pain.
Article
Neurosciences
Laura Medlock, Kazutaka Sekiguchi, Sungho Hong, Salvador Dura-Bernal, William W. Lytton, Steven A. Prescott
Summary: Pain-related sensory input is processed in the spinal dorsal horn (SDH) before being relayed to the brain, influencing how stimuli are perceived as painful. Researchers developed a computational model constrained by experimental data to explore SDH function, which reproduced characteristic firing patterns of spinal neurons and responded consistently to inhibition reduction and specific neuron type ablation.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Anesthesiology
Zhiqiang Pan, Qi Zhang, Xiaodan Liu, Huimin Zhou, Tong Jin, Ling-Yun Hao, Ling Xie, Ming Zhang, Xiao-Xiao Yang, Meng-Lan Sun, Zhou-Ya Xue, Yang Tao, Xin-Chun Ye, Wen Shen, Jun-Li Cao
Summary: The study reveals that downregulation of spinal METTL3 and coordination with YTHDF2 contribute to the modulation of inflammatory pain through stabilizing upregulation of TET1 in spinal neurons. Manipulating Mett13 levels can affect pain behavior, and decreased levels of YTHDF2 can lead to increased TET1 levels in the spinal cord and influence pain regulation.
Article
Biochemistry & Molecular Biology
Jingjing Zhang, Lina Rong, Jinping Shao, Yidan Zhang, Yaping Liu, Sen Zhao, Lei Li, Wenli Yu, Mengya Zhang, Xiuhua Ren, Qingzan Zhao, Changlian Zhu, Huan Luo, Weidong Zang, Jing Cao
Summary: The study demonstrates that miR-137-mediated Kv1.2 impairment plays a crucial role in the pathogenesis of nerve injury-induced neuropathic pain, suggesting it as a novel potential therapeutic target for neuropathic pain management.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Anesthesiology
Noemie Mermet-Joret, Cristina Alba-Delgado, Amelie Descheemaeker, Francois Gabrielli, Radhouane Dallel, Myriam Antri
Summary: Pain processing is immature in young mammals. The development of neurons in the medullary dorsal horn (MDH) has been overlooked. In this study, we investigate the age-dependent development of neurons in the inner lamina II (IIi) of the MDH in rats. We found that these neurons undergo physiological and structural changes, which are associated with facial mechanical allodynia.
Review
Biochemistry & Molecular Biology
Jonathan Chabert, Etienne Allauze, Bruno Pereira, Carine Chassain, Ingrid De Chazeron, Jean-Yves Rotge, Philippe Fossati, Pierre-Michel Llorca, Ludovic Samalin
Summary: The levels of neurometabolites such as Glutamate, Glutamine, Glx, and N-acetylaspartate in various brain regions differ in patients with bipolar disorder. The altered levels of NAA and Gln may be associated with the pathogenesis of bipolar disorder.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Nutrition & Dietetics
Julie Veziant, Mathilde Bonnet, Bob Occean, Chadly Dziri, Bruno Pereira, Karem Slim
Summary: This systematic review and meta-analysis aimed to evaluate the effectiveness of probiotics/synbiotics in reducing infectious complications after colorectal surgery and explore their potential as perioperative measures. The results showed that probiotics/synbiotics significantly reduced infectious complications, particularly in pulmonary and urinary infections. However, further research is needed to address practical questions regarding the formulation and duration of probiotics/synbiotics before their inclusion in enhanced recovery programs.
Review
Environmental Sciences
Remi Colin-Chevalier, Frederic Dutheil, Sebastien Cambier, Samuel Dewavrin, Thomas Cornet, Julien Steven Baker, Bruno Pereira
Summary: Technological advances and digital tools have enabled researchers to collect large amounts of health data for clinical research. Real-world data, which complements traditional randomized trials, is becoming increasingly important in health decisions. However, the analysis of this longitudinal data faces methodological challenges, including cluster-correlated data, missing data, and longitudinal data issues. This article presents various solutions from the literature to address these challenges in the context of occupational health data.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Nutrition & Dietetics
Jean-Baptiste Bouillon-Minois, Louisa Khaled, Florence Vitte, Ludovic Miraillet, Romain Eschalier, Matthieu Jabaudon, Vincent Sapin, Lucas Derault, Samy Kahouadji, Marina Brailova, Julie Durif, Jeannot Schmidt, Fares Moustafa, Bruno Pereira, Emmanuel Futier, Damien Bouvier
Summary: The study establishes the reference values for ionized magnesium (iMg) and finds a strong correlation between plasmatic magnesium (pMg) and iMg. The interpretation of iMg is not influenced by pH and albumin variations. The incidencce of ionized hypomagnesemia among de novo atrial fibrillation patients is 8.5%.
Article
Environmental Sciences
Alicia Fillon, Nicole Fearnbach, Stephanie Vieira, Jade Gelinier, Sarah Bagot, Melina Bailly, Audrey Boscaro, Lena Pelissier, Julie Siroux, Vincent Grasteau, Jean Bertsch, Bruno Pereira, Martine Duclos, Celine Lambert, David Thivel
Summary: This study evaluates the effects of the Globe Trotter Initiative on physical activity level, sedentary time, physical fitness, and activity preferences in primary school children. The findings suggest that the intervention had beneficial effects on reducing sedentary time and increasing preference for physical activities among the participating students.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Review
Environmental Sciences
Frederic Dutheil, Tharwa Oueslati, Louis Delamarre, Joris Castanon, Caroline Maurin, Frederic Chiambaretta, Julien S. S. Baker, Ukadike C. C. Ugbolue, Marek Zak, Ines Lakbar, Bruno Pereira, Valentin Navel
Summary: Myopia is a global public health problem that affects people's quality of life and work productivity. This study conducted a meta-analysis to investigate the effects of near work on myopia, including adults' occupational exposure. The results showed that near work conditions, including occupational exposure, were associated with myopia.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Medicine, General & Internal
Randy Coupet, Martin Schlaepfer, Thomas A. Neff, Pierre Boucher, Pierre Bailly, Martin Bellgardt, Rafael Badenes, Jose Carbonell, Tobias Becher, Caroline Varillon, Dominique Morand, Raiko Blondonnet, Jean-Michel Constantin, Bruno Pereira, Brian O'Gara, Matthieu Jabaudon
Summary: This study investigated 196 COVID-19 ARDS patients from 10 intensive care units in Europe and the US in 2021. The results showed that inhaled sedation did not significantly improve the number of ventilator-free days compared to intravenous sedation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Eve Faugeras, Lauren Veronese, Gaelle Jeannin, Henri Janicot, Sebastien Bailly, Jacques-Olivier Bay, Bruno Pereira, Anne Cayre, Frederique Penault-Llorca, Florent Cachin, Patrick Merle, Andrei Tchirkov
Summary: In patients with advanced non-small cell lung cancer (NSCLC), short telomeres, high levels of telomerase, and aberrant expression of shelterin genes TRF2, RAP1, and TIN2 are significantly associated with shorter survival. The determination of telomere parameters in NSCLC could be useful for individualized treatment decisions.
Article
Biochemistry & Molecular Biology
Xavier Poirot-Seynaeve, Perrine Smets, Bruno Pereira, Louis Olagne, Julien Stievenart, Vincent Sapin, Olivier Aumaitre, Marc Andre, Ludovic Trefond
Summary: Procalcitonin (PCT) may be useful for differentiating between infections and flare in patients suffering from antineutrophil-cytoplasmic-antibody (ANCA)-associated vasculitides (AAV). The study found that PCT levels were significantly higher in the infected group compared to the relapsing group. C-reactive protein (CRP) levels were also higher in cases of infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Charlene Gouhier, Hanae Pons-Rejraji, Sandra Dollet, Laure Chaput, Celine Bourgne, Marc Berger, Bruno Pereira, Andrei Tchirkov, Florence Brugnon
Summary: No significant impact of slow freezing on sperm telomere length (STL) was observed, despite increased sperm DNA oxidation and fragmentation. These findings are important for ensuring the safety of assisted reproductive techniques, fertility preservation, and sperm donation.
Article
Medicine, General & Internal
Amandine Puissegur, Marie Accoceberry, Marion Rouzaire, Bruno Pereira, Marie Herault, Clement Bruhat, Amelie Delabaere, Denis Gallot
Summary: We conducted a retrospective case-control study to identify risk factors for early perineal suture breakdown in patients with perineal tear or episiotomy complication. Instrumental delivery and a longer second phase of labor were confirmed as risk factors for early perineal suture breakdown.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Oncology
Julie Maraud, Sabrina Bedhomme, Bruno Pereira, Sophie Trevis, Marine Jary, David Balayssac
Summary: Self-medication practices by cancer patients and survivors, including the use of dietary supplements and pain medications, were assessed in this French observational study. The study found that self-medication was commonly practiced to manage the adverse effects of anticancer therapies and to improve their efficacy. Patients had high confidence in the safety of self-medication, but it was associated with altered social functioning, pain, insomnia, and financial difficulties. These findings highlight the potential undermanagement of cancer and treatment-related adverse effects.
Article
Oncology
Nicolas Saroul, Nicolas Tardif, Bruno Pereira, Alexis Dissard, Laura Montrieul, Phelipe Sanchez, Jerome Salles, Jens Erik Petersen, Towe Jakobson, Laurent Gilain, Thierry Mom, Yves Boirie, Olav Rooyakers, Stephane Walrand
Summary: Cancer cachexia, particularly muscle loss, in head and neck cancer (HNC) is not solely attributed to decreased food intake. The exact role of secreted factors from tumor cells in driving cancer cachexia is still unknown. This study used conditioned media from HNC cell lines and mix of sera from HNC patients to analyze their impact on skeletal muscle protein catabolism, and found similar metabolic responses in both cases. These findings suggest that muscle atrophy in HNC patients is not solely caused by reduced food intake.
Article
Pediatrics
Justine Paysal, Charlotte Oris, Ugo Troin, Pierre-Nicolas Limeri, Jeanne Allard, Marie Tadrent, Bruno Pereira, Etienne Merlin, Emmanuelle Rochette, Bertrand Evrard, Julie Durif, Vincent Sapin, Maguelonne Pons
Summary: This study aimed to compare the levels of blood tryptase and fecal calprotectin in newborns according to their term, trophicity, and sex. The results showed that blood tryptase levels were higher in premature newborns and in situations of antenatal use of corticosteroids and non-exclusive use of human milk. However, only prematurity significantly influenced tryptase levels according to regression analyses. Fecal calprotectin levels were much higher in female newborns compared to male newborns. The differences in tryptase levels could be due to early aggression of the still-immature digestive wall in premature newborns, while the influence of sex on fecal calprotectin levels remains unexplained.