Article
Biochemistry & Molecular Biology
Binbin Tao, Hongling Hu, Ji Chen, Lu Chen, Daji Luo, Yonghua Sun, Feng Ge, Zuoyan Zhu, Vance L. Trudeau, Wei Hu
Summary: Recent research has identified a new subunit of the SIN3A-HDAC complex, called Sinhcaf, that plays a crucial role in germ plasm aggregation and primordial germ cell (PGC) specification. Sinhcaf-dependent histone deacetylation is essential for proper PGC development, as mutations in sinhcaf result in defects in germ plasm aggregation and decreased PGC abundance. Sinhcaf achieves this by regulating the acetylation status of the kif26ab promoter, which affects its transcription and ultimately PGC specification.
Article
Cell Biology
Komeil Razmi, Jawahar G. Patil
Summary: This study investigated the spatio-temporal expression of teleostean germ plasm markers in Gambusia holbrooki, and found that both maternal inheritance mode and strictly zygotic activation mode exist in this species. The PGC clustering, migration, and colonization patterns of G. holbrooki resemble those of zebrafish, medaka, and mice, indicating evolutionary similarities. Furthermore, the expression domains of PGC markers in G. holbrooki show both teleost-specific and murine model-specific characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Komeil Razmi, Jawahar G. Patil
Summary: This study investigates the expression of germ plasm markers during embryonic development in Gambusia holbrooki, and suggests that the reproductive developmental adaptations in this species may reflect a transition from lecithotrophy to matrotrophy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Developmental Biology
Grace Hancock, Sissy E. Wamaitha, Lior Peretz, Amander T. Clark
Summary: The peri-implantation window of mammalian development is crucial for primordial germ cell (PGC) specification. While preimplantation dynamics are relatively conserved between species, the implantation window marks a stage of developmental divergence, requiring the combined use of human and nonhuman primate embryos to determine the origin of PGCs.
Article
Developmental Biology
Madeline Cassani, Geraldine Seydoux
Summary: Specification of the germ line in Caenorhabditis elegans involves two independent cytoplasmic condensates that independently enrich and regulate maternal mRNAs, playing a crucial role in embryonic development.
Article
Biology
Yasunari Seita, Keren Cheng, John R. McCarrey, Nomesh Yadu, Ian H. Cheeseman, Alec Bagwell, Corinna N. Ross, Isamar Santana Toro, Li-hua Yen, Sean Vargas, Christopher S. Navara, Brian P. Hermann, Kotaro Sasaki
Summary: This study successfully established a method for culturing mid-gestation mouse iPSCs on a feeder layer, which can further differentiate into cells similar to primordial germ cells (PGCLCs). Furthermore, these cells can acquire an early developmental germ cell phenotype when co-cultured with mouse testicular somatic cells.
Article
Cell Biology
Andreas Zaucker, Claire A. Mitchell, Helena L. E. Coker, Karuna Sampath
Summary: During the early stages of zebrafish development, germplasm exhibits highly dynamic behavior. New research methods and tools provide efficient sample mounting strategies for high-resolution imaging of early embryos, facilitating the study of germ granule dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alexander A. Tokmakov, Yudai Morichika, Ryuga Teranishi, Ken-Ichi Sato
Summary: This study demonstrates that oxidative stress can induce overactivation of frog eggs, which is a calcium-dependent phenomenon and can be attenuated by the selective calcium chelator BAPTA. Several intracellular events, such as degradation of cyclin B2 and decline in mitochondrial membrane potential, occur in overactivated eggs. Furthermore, plasma membrane integrity is compromised in overactivated eggs.
Article
Biochemistry & Molecular Biology
Qisheng Zuo, Jin Jing, Jing Zhou, Yani Zhang, Wanhong Wei, Guohong Chen, Bichun Li
Summary: The study identifies a long noncoding RNA called LncBMP4 that promotes BMP4 expression in chicken primordial germ cells (PGCs) formation through various mechanisms, thereby facilitating PGC formation and migration.
Review
Cell Biology
Christina L. Hansen, Francisco Pelegri
Summary: This review article discusses the differentiation of primordial germ cells in early embryonic development in sexually reproducing species. It reviews two main mechanisms of PGC specification, shared molecular characteristics, and the distribution of germ cell determination systems across different animal species. Ongoing investigations and potential lines of inquiry into the evolutionary relationships between different modes of specification are also briefly highlighted.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Endocrinology & Metabolism
Yuta Tanizaki, Yuki Shibata, Wonho Na, Yun-Bo Shi
Summary: Amphibian metamorphosis, especially in anurans like Xenopus laevis and Xenopus tropicalis, is a valuable model to study how T3 regulates adult organ development due to its similarity to mammalian postembryonic development and its independence from maternal influence. Studies on intestinal remodeling have provided important insights into the molecular mechanisms of T3 regulation of cell fate during development, suggesting that T3-induced activation of the cell cycle program is crucial for larval epithelial cell death and the formation of adult intestinal stem cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Jonathan J. Carver, Yuanfa He, Yong Zhu
Summary: The study shows that Adamts9 plays a conserved role in germ cell migration among invertebrates and vertebrates. It is suggested that Adamts9 is not essential for germ cell migration in vertebrates due to the presence of other metalloproteinases that may compensate for its function. Further research is needed to explore the functions and mechanisms of metalloproteinases in germ cell migration and gonad formation in vertebrates.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Lei Li, Ying Xu, Jihong Zheng, Zhe Kuang, Cong Zhang, Na Li, Gufa Lin, Chao Zhang
Summary: The study revealed a specific regulatory pattern of the melanocortin system in Xenopus laevis, where MRAP2 proteins directly interact with MC4R proteins on the cell surface in a functional antiparallel dimeric topology.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Weiyan Yuan, Zhaokai Yao, Veeramohan Veerapandian, Xinyan Yang, Xiaoman Wang, Dingyao Chen, Linzi Ma, Chaohui Li, Yi Zheng, Fang Luo, Xiao-yang Zhao
Summary: The histone demethylase KDM2B is found to be highly expressed in male fetal germ cells (FGCs) and shares a similar expression pattern with hPGC marker genes in hPGCLCs. KDM2B plays an essential role in hPGCLC specification by suppressing somatic gene expression during primordial germ cell development.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kirill S. Tenkov, Mikhail V. Dubinin, Alexander A. Vedernikov, Yuliya A. Chelyadnikova, Konstantin N. Belosludtsev
Summary: The present study investigates the in vivo effect of triclosan on frogs and reveals a dose-dependent impact on their survival. Triclosan damages the liver tissue, reduces the osmotic resistance of red blood cells, and suppresses the respiration rate and calcium retention capacity of frog liver mitochondria. These findings highlight the significance of triclosan on aquatic organisms.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)