Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library
Published 2020 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library
Authors
Keywords
-
Journal
BMC BIOLOGY
Volume 18, Issue 1, Pages -
Publisher
Springer Science and Business Media LLC
Online
2020-11-24
DOI
10.1186/s12915-020-00905-1
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
- (2020) Georg Michlits et al. NATURE METHODS
- Guidelines for optimized gene knockout using CRISPR/Cas9
- (2019) Claude Van Campenhout et al. BIOTECHNIQUES
- Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens
- (2019) Fiona M. Behan et al. NATURE
- CRISPR knockout screen implicates three genes in lysosome function
- (2019) Guy M. Lenk et al. Scientific Reports
- Systematic characterization of BAF mutations provides insights into intracomplex synthetic lethalities in human cancers
- (2019) Sandra Schick et al. NATURE GENETICS
- Identification of the Cell-Surface Protease ADAM9 as an Entry Factor for Encephalomyocarditis Virus
- (2019) Jim Baggen et al. mBio
- Biological plasticity rescues target activity in CRISPR knock outs
- (2019) Arne H. Smits et al. NATURE METHODS
- Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
- (2019) Daqi Wang et al. Nature Communications
- Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets
- (2019) Joshua M. Dempster et al. Nature Communications
- Improved design and analysis of CRISPR knockout screens
- (2018) Chen-Hao Chen et al. BIOINFORMATICS
- Past, present, and future of Bcr-Abl inhibitors: from chemical development to clinical efficacy
- (2018) Federico Rossari et al. Journal of Hematology & Oncology
- Haploid genetic screens identify genetic vulnerabilities to microtubule-targeting agents
- (2018) Nora M. Gerhards et al. Molecular Oncology
- Defining essential genes for human pluripotent stem cells by CRISPR–Cas9 screening in haploid cells
- (2018) Atilgan Yilmaz et al. NATURE CELL BIOLOGY
- CRISPR–Cas9 genome editing in human cells occurs via the Fanconi anemia pathway
- (2018) Chris D. Richardson et al. NATURE GENETICS
- Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48
- (2018) Georgia Velimezi et al. Nature Communications
- Ensembl 2019
- (2018) Fiona Cunningham et al. NUCLEIC ACIDS RESEARCH
- Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
- (2018) Kendall R. Sanson et al. Nature Communications
- Phenotype databases for genetic screens in human cells
- (2017) Benedikt Rauscher et al. JOURNAL OF BIOTECHNOLOGY
- Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells
- (2017) Robin M Meyers et al. NATURE GENETICS
- Emerging and evolving concepts in gene essentiality
- (2017) Giulia Rancati et al. NATURE REVIEWS GENETICS
- Am I ready for CRISPR? A user's guide to genetic screens
- (2017) John G. Doench NATURE REVIEWS GENETICS
- A p53-dependent response limits the viability of mammalian haploid cells
- (2017) Teresa Olbrich et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia
- (2017) Martin Moder et al. Nature Communications
- Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
- (2017) Traver Hart et al. G3-Genes Genomes Genetics
- BAGEL: a computational framework for identifying essential genes from pooled library screens
- (2016) Traver Hart et al. BMC BIOINFORMATICS
- Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
- (2016) John G Doench et al. NATURE BIOTECHNOLOGY
- Genome-wide CRISPR screens reveal a Wnt–FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors
- (2016) Zachary Steinhart et al. NATURE MEDICINE
- The Fanconi anaemia pathway: new players and new functions
- (2016) Raphael Ceccaldi et al. NATURE REVIEWS MOLECULAR CELL BIOLOGY
- GenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens
- (2016) Benedikt Rauscher et al. NUCLEIC ACIDS RESEARCH
- CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
- (2016) S. John Liu et al. SCIENCE
- CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions
- (2016) D. M. Munoz et al. Cancer Discovery
- Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
- (2016) A. J. Aguirre et al. Cancer Discovery
- The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells
- (2016) René M. Daer et al. ACS Synthetic Biology
- A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia
- (2016) Konstantinos Tzelepis et al. Cell Reports
- Nucleosome breathing and remodeling constrain CRISPR-Cas9 function
- (2016) R Stefan Isaac et al. eLife
- Nucleosomes impede Cas9 access to DNA in vivo and in vitro
- (2016) Max A Horlbeck et al. eLife
- Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
- (2015) Sidi Chen et al. CELL
- High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
- (2015) Traver Hart et al. CELL
- Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains
- (2015) Junwei Shi et al. NATURE BIOTECHNOLOGY
- Orchestrating high-throughput genomic analysis with Bioconductor
- (2015) Wolfgang Huber et al. NATURE METHODS
- limma powers differential expression analyses for RNA-sequencing and microarray studies
- (2015) Matthew E. Ritchie et al. NUCLEIC ACIDS RESEARCH
- Gene essentiality and synthetic lethality in haploid human cells
- (2015) V. A. Blomen et al. SCIENCE
- Identification and characterization of essential genes in the human genome
- (2015) T. Wang et al. SCIENCE
- Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins
- (2015) Eric M. Davis et al. Cell Reports
- mixtools: AnRPackage for Analyzing Finite Mixture Models
- (2015) Tatiana Benaglia et al. Journal of Statistical Software
- Measuring error rates in genomic perturbation screens: gold standards for human functional genomics
- (2014) T. Hart et al. Molecular Systems Biology
- Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
- (2014) John G Doench et al. NATURE BIOTECHNOLOGY
- Improved vectors and genome-wide libraries for CRISPR screening
- (2014) Neville E Sanjana et al. NATURE METHODS
- E-CRISP: fast CRISPR target site identification
- (2014) Florian Heigwer et al. NATURE METHODS
- Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
- (2013) Baohui Chen et al. CELL
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
- (2013) Ophir Shalem et al. SCIENCE
- Genetic Screens in Human Cells Using the CRISPR-Cas9 System
- (2013) Tim Wang et al. SCIENCE
- 5T4 oncofetal antigen is expressed in high risk of relapse childhood pre-B acute lymphoblastic leukemia and is associated with a more invasive and chemotactic phenotype
- (2012) F V Castro et al. LEUKEMIA
- Fast gapped-read alignment with Bowtie 2
- (2012) Ben Langmead et al. NATURE METHODS
- clusterProfiler: an R Package for Comparing Biological Themes Among Gene Clusters
- (2012) Guangchuang Yu et al. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Global gene disruption in human cells to assign genes to phenotypes by deep sequencing
- (2011) Jan E Carette et al. NATURE BIOTECHNOLOGY
- The art and design of genetic screens: RNA interference
- (2008) Michael Boutros et al. NATURE REVIEWS GENETICS
- Homogeneity and persistence of transgene expression by omitting antibiotic selection in cell line isolation
- (2008) W. L. Kaufman et al. NUCLEIC ACIDS RESEARCH
- A One Pot, One Step, Precision Cloning Method with High Throughput Capability
- (2008) Carola Engler et al. PLoS One
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreCreate your own webinar
Interested in hosting your own webinar? Check the schedule and propose your idea to the Peeref Content Team.
Create Now