Article
Critical Care Medicine
Adrian A. N. Doevelaar, Martin Bachmann, Bodo Hoelzer, Felix S. Seibert, Benjamin J. Rohn, Frederic Bauer, Oliver Witzke, Ulf Dittmer, Michael Bachmann, Serap Yilmaz, Rita Dittmer, Sonja Schneppenheim, Nina Babel, Ulrich Budde, Timm H. Westhoff
Summary: The study suggests that COVID-19 patients have significantly increased levels of von Willebrand factor, potentially leading to a relative deficiency of ADAMTS13 and the formation of large von Willebrand factor multimers, similar to thrombotic thrombocytopenic purpura. The ADAMTS13/von Willebrand factor antigen ratio is an independent predictor of disease severity and mortality, highlighting the potential use of plasma exchange as a therapeutic option and the inclusion of these factors in diagnostic workup for COVID-19.
CRITICAL CARE MEDICINE
(2021)
Article
Hematology
Masayuki Kubo, Kazuya Sakai, Masaki Hayakawa, Hirokazu Kashiwagi, Hideo Yagi, Yoshinobu Seki, Atsushi Hasegawa, Haruyuki Tanaka, Itsuto Amano, Yoshiaki Tomiyama, Masanori Matsumoto
Summary: This study investigated whether VWF degradation is enhanced in patients with ET. The results showed that patients with higher platelet counts had lower HMW-VWFM index and higher VWF-DP:VWF Ag ratio. Cytoreductive therapy increased the HMW-VWFM index and decreased the VWF-DP:VWF Ag ratio. In conclusion, an increased platelet count in ET patients is associated with enhanced cleavage of VWF, and cytoreductive therapy can reduce this cleavage and improve VWFM distributions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Medicine, General & Internal
Jun Kit He, Samuel Schick, Marshall Williams, Bradley Wills, Martim Pinto, Gean Viner, Eugene Brabston, Amit Momaya, X. Long Zheng, Brent Ponce
Summary: This study found that following TSA surgery, patients had decreased ADAMTS13 activity, increased VWF levels, and the lowest ADAMTS13/VWF ratio, suggesting that the highest risk for VTE may be on postoperative day 2. Proper anticoagulation treatment is recommended during this period.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Hematology
Miruna Popa, Markus Hecker, Andreas H. Wagner
Summary: ADAMTS13 is a metalloprotease whose expression is inhibited by proinflammatory cytokines, potentially increasing the risk of arterial thrombosis. Factors associated with atherosclerosis do not seem to affect ADAMTS13 expression. There is a reciprocal correlation between the expression of ADAMTS13 and vWF in endothelial cells.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Ilaria Mancini, Luciano Baronciani, Andrea Artoni, Paola Colpani, Marina Biganzoli, Giovanna Cozzi, Cristina Novembrino, Massimo Boscolo Anzoletti, Valentina De Zan, Maria Teresa Pagliari, Roberta Gualtierotti, Stefano Aliberti, Mauro Panigada, Giacomo Grasselli, Francesco Blasi, Flora Peyvandi
Summary: Severe COVID-19 patients exhibit an increased risk of thromboembolic events, with evidence of microthrombosis in the lungs of deceased patients. The study found a significant alteration of the VWF-ADAMTS13 axis in COVID-19 patients, with an elevated VWF:Ag to ADAMTS13 activity ratio strongly associated with disease severity, enhancing the hypercoagulable state and risk of microthrombosis in these patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Stephen J. X. Murphy, Soon Tjin Lim, Fionnuala Hickey, Justin A. Kinsella, Deirdre R. Smith, Sean Tierney, Bridget Egan, T. Martin Feeley, Sinead M. Murphy, D. Ronan Collins, Tara Coughlan, Desmond O'Neill, Joseph A. Harbison, Prakash Madhavan, Sean M. O'Neill, Mary-Paula Colgan, James S. O'Donnell, Jamie M. O'Sullivan, George Hamilton, Dominick J. H. McCabe
Summary: The study found that VWF:Ag levels were higher and VWFpp/VWF:Ag ratios were lower in early symptomatic patients with carotid stenosis compared to asymptomatic patients, and these levels decreased over time. Additionally, there was a difference in VWF:Ag levels between patients with blood group O and those without blood group O.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Multidisciplinary Sciences
Kanwal Singh, Andrew C. Kwong, Hasam Madarati, Sharumathy Kunasekaran, Taylor Sparring, Alison E. Fox-Robichaud, Patricia C. Liaw, Colin A. Kretz
Summary: The study found that in septic patients, ADAMTS13 activity and antigen were reduced, while VWF antigen was elevated, with non-septic patients showing milder changes. These abnormalities persisted throughout the ICU stay and discharge in all patient cohorts, potentially contributing to a prothrombotic state in sepsis patients.
Editorial Material
Hematology
Colin A. Kretz
Summary: The study introduces a novel thrombolytic agent, Microlyse, which targets von Willebrand factor to activate plasminogen and has potential therapeutic applications for acute episodes of TTP.
Article
Engineering, Chemical
Katarzyna I. Jankowska, Upendra Katneni, Brian C. Lin, Randilu Amarasinghe, Je-Nie Phue, Wells W. Wu, Nobuko Hamasaki-Katagiri, Wojciech Jankowski, Rong-Fong Shen, Chava Kimchi-Sarfaty
Summary: ADAMTS13 is an important hemostatic enzyme that plays a role in preventing excessive blood clotting. Deficiency in ADAMTS13 can lead to a condition called thrombotic thrombocytopenic purpura (TTP). Recent studies have shown a correlation between low levels of ADAMTS13 and increased mortality in patients with COVID-19. Therefore, investigating ADAMTS13 as a potential therapeutic option is of great interest in the field of hematology. This study successfully developed a method to purify highly active recombinant ADAMTS13 from cell culture media, providing a foundation for further laboratory-scale studies.
Article
Hematology
Berangere S. Joly, Michael Darmon, Charlotte Dekimpe, Thibault Dupont, Guillaume Dumas, Elise Yvin, Nicolas Beranger, Karen Vanhoorelbeke, Elie Azoulay, Agnes Veyradier
Summary: Clinical features, hemostatic laboratory parameters, VWF/ADAMTS13 axis, and ADAMTS13 conformation were investigated in critically ill COVID-19 patients at admission. The study found that an imbalance between high VWF antigen levels and normal or slightly decreased ADAMTS13 activity levels correlated with mortality, suggesting that these markers may reflect inflammation and endothelial damage rather than driving the thrombotic process of COVID-19.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Hematology
Liang Zheng, Liyun Cao, X. Long Zheng
Summary: This study reveals the protective role of ADAMTS13 or lack of VWF in reducing irradiation and melanoma-induced thrombotic microangiopathy and mortality. The microenvironment created by plasma ADAMTS13 or lack of VWF may affect melanoma cell phenotypes through alteration of oxidation-reduction and lipid metabolic pathways.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Shannen J. Deconinck, Christoph Nix, Svenja Barth, Eveline Bennek-Schoepping, Antoine Rauch, An-Sofie Schelpe, Elien Roose, Hendrik B. Feys, Inge Pareyn, Aline Vandenbulcke, Joshua Muia, Christophe Vandenbriele, Sophie Susen, Bart Meyns, Claudia Tersteeg, Steven Jacobs, Simon F. De Meyer, Karen Vanhoorelbeke
Summary: Acquired von Willebrand syndrome (aVWS) is common in patients with mechanical circulatory support devices and can lead to bleeding complications. Our study demonstrates that inhibiting ADAMTS13 with an anti-ADAMTS13 monoclonal antibody can prevent the degradation of von Willebrand factor (VWF) and improve hemostasis in these patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Health Care Sciences & Services
Chanukya K. Colonne, Emmanuel J. Favaloro, Leonardo Pasalic
Summary: von Willebrand factor (VWF) and ADAMTS13 play important roles in normal hemostasis and can be involved in abnormal hemostasis in malignancy. VWF may also contribute to inflammation, angiogenesis, tumor biology, and tumor metastasis. VWF and ADAMTS13 have been explored as tumor biomarkers, but their clinical utility is currently limited. Further studies are needed to understand the relationships between VWF, ADAMTS13, and malignancy.
Review
Hematology
Dafna J. Groeneveld, Lauren G. Poole, James P. Luyendyk
Summary: Liver disease is associated with significant changes in the hemostatic system, with anticoagulants showing promise in slowing disease progression but also posing bleeding risks. Elevated levels of VWF may accelerate intrahepatic thrombosis, suggesting a potential link between VWF function and the progression of liver disease.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Urology & Nephrology
Gurbey Ocak, Mark Roest, Marianne C. Verhaar, Maarten B. Rookmaaker, Peter J. Blankestijn, Willem Jan W. Bos, Rob Fijnheer, Nathalie C. Pequeriaux, Friedo W. Dekker
Summary: In dialysis patients, high VWF levels and low ADAMTS13 activity are associated with increased mortality risks.
Letter
Hematology
Li Li, Dana Huskens, Lisa Florin, Bas de Laat, Mark Roest, Katrien M. J. Devreese
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Article
Hematology
Godfrey S. Temba, Nadira Vadaq, Jun Wan, Vesla Kullaya, Dana Huskens, Tal Pecht, Martin Jaeger, Collins K. Boahen, Vasiliki Matzaraki, Wieteke Broeders, Leo A. B. Joosten, Sultana M. H. Faradz, Gibson Kibiki, Saskia Middeldorp, Duccio Cavalieri, Paolo Lionetti, Philip G. de Groot, Joachim L. Schultze, Mihai G. Netea, Vinod Kumar, Bas de Laat, Blandina T. Mmbaga, Andre J. van der Ven, Mark Roest, Quirijn de Mast
Summary: There are geographical differences in coagulation between healthy Tanzanian and Dutch individuals, which may be influenced by inflammation, genetics, and diet.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Nikolett Wohner, Silvie Sebastian, Vincent Muczynski, Dana Huskens, Bas de Laat, Philip G. de Groot, Peter J. Lenting
Summary: The study reveals that VWF strings secreted from endothelial cells are only partially covered with platelets, and osteoprotegerin plays a role in regulating platelet adhesion to VWF. This regulatory mechanism may be more relevant under inflammatory conditions.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
B. de Laat, M. J. M. Traets, R. W. M. De Laat-Kremers, S. P. Verweij, M. Ninivaggi, E. Jong, D. Huskens, B. A. Blok, G. C. P. Remme, A. Miszta, R. H. T. Nijhuis, G. J. M. Herder, R. Fijnheer, M. Roest, A. T. L. Fiolet, J. A. Remijn
Summary: This study investigated the differences in blood coagulation between PCR positive and negative COVID-19 patients. The results showed that PCR positive patients had a pronounced prothrombotic phenotype, mainly due to increased endothelial activation upon hospital admission. These findings suggest that coagulation tests may be useful in identifying severe cases of COVID-19 at risk for thrombosis.
Article
Hematology
Li Li, Mark Roest, Joost C. M. Meijers, Bas de Laat, Rolf T. Urbanus, Philip G. de Groot, Dana Huskens
Summary: Collagen activates platelets to initiate coagulation independently of certain clotting factors, and the activation of glycoprotein VI on platelets leads to the initiation of thrombin generation through platelet-derived FIXa activity.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Shengshi Huang, Wouter van der Heijden, Isaie J. J. Reuling, Jun Wan, Qiuting Yan, Romy M. W. Kremers, Andre J. J. van der Ven, Philip G. G. de Groot, Matthew McCall, Robert W. W. Sauerwein, Teun Bousema, Mark Roest, Marisa Ninivaggi, Quirijn de Mast, Bas de Laat
Summary: Decreased platelet count is observed during asexual Plasmodium falciparum parasitemia, but its association with platelet and coagulation function changes is unknown. The impact of gametocytemia on platelets and coagulation remains unclear as well.
Article
Cardiac & Cardiovascular Systems
Bas de Laat, Hendrik Stragier, Romy de Laat-Kremers, Marisa Ninivaggi, Dieter Mesotten, Steven Thiessen, Kristien Van Pelt, Mark Roest, Joris Penders, Pascal Vanelderen, Dana Huskens, Raf De Jongh, Margot Vander Laenen, Tom Fivez, Hugo ten Cate, Rene Heylen, Line Heylen, Deborah Steensels
Summary: A study found that after receiving the ChAdOx1-S vaccine, the levels of prothrombin and fibrinogen in the blood significantly decreased. Vaccinated individuals had a procoagulant profile in thrombin generation, along with endothelial activation and persistent immune system activity, which could increase the risk of thrombotic events.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Romy de Laat-Kremers, Augusto Di Castelnuovo, Lisa van der Vorm, Simona Costanzo, Marisa Ninivaggi, Chiara Cerletti, Dana Huskens, Amalia De Curtis, Alessandro Gialluisi, Cuicui Bai, Giovanni de Gaetano, Dongmei Yin, Maria Benedetta Donati, Bas de Laat, Licia Iacoviello, Moli Sani Invest
Summary: The study found significant associations between high BMI and blood lipid levels with increased TG parameters, suggesting that this hypercoagulability may partially explain the increased risk of cardiovascular disease in individuals with obesity and/or dyslipidemia.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cardiac & Cardiovascular Systems
Li Li, Mark Roest, Yaqiu Sang, Jasper A. Remijn, Rob Fijnheer, Karel Smit, Dana Huskens, Jun Wan, Bas de Laat, Joke Konings
Summary: Patients with multiple myeloma have imbalanced blood cell metabolism and WB-TG profile, which may explain the paradoxically high prevalence of bleeding symptoms compared to an increased thrombosis risk. Disturbance is not observed in plasma TG, indicating that blood cells are the major determinants for the imbalanced hemostasis in multiple myeloma patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Licia Iacoviello, Romy De Laat-Kremers, Simona Costanzo, Qiuting Yan, Augusto Di Castelnuovo, Lisa van der Vorm, Amalia De Curtis, Marisa Ninivaggi, Chiara Cerletti, Maria Benedetta Donati, Bas de Laat
Summary: This study investigated the association between antithrombin (AT) levels and mortality in the general population. The results showed that low AT levels are associated with a lower risk of cardiovascular-related mortality, but a higher risk of cancer mortality.
Article
Hematology
Fadel Muhammad Garishah, Dana Huskens, Setyo Gundi Pramudo, Dessy Andriani, Mila Astrilia, Rizki Akbar Sentosa, Andre J. A. M. van der Ven, Bas de Laat, Muhammad Hussein Gasem, Quirijn de Mast, Mark Roest
Summary: This study investigates the ATP release kinetics of platelets in hospitalized COVID-19 patients and finds that platelet hyper-responsiveness and reduced platelet GRC are associated with critical illness and increased mortality.
THROMBOSIS AND HAEMOSTASIS
(2022)
Meeting Abstract
Hematology
Romy de Laat, Augusto Di Castelnuovo, Lisa van der Vorm, Simona Costanzo, Marisa Ninivaggi, Chiara Cerletti, Dana Huskens, Amalia De Curtis, Alessandro Gialluisi, Cuicui Bai, Giovanni de Gaetano, Dongmei Yin, Maria Benedatta Donati, Bas de Laat, Licia Iacoviello
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2023)
Meeting Abstract
Gastroenterology & Hepatology
Marilena Stamouli, Jelle Adelmeijer, Dana Huskens, Savannah Rivera, Kevin O'Reilly, Elena Palma, Neil Youngson, William Bernal, Shilpa Chokshi, Mark Roest, Ton Lisman, Vishal Patel
JOURNAL OF HEPATOLOGY
(2022)
