Review
Pharmacology & Pharmacy
Kanchana K. Ayyar, Alan C. Moss
Summary: Exosomes are small vesicles released by cells, containing a variety of molecules, and can be found in various tissues of humans and other mammals. A unique exosome profile can be detected in blood and fecal samples of patients with Inflammatory Bowel Disease (IBD), making them potential targets for IBD therapy.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Cell Biology
Christopher R. Pitzer, Hector G. Paez, Stephen E. Alway
Summary: Cancer cachexia is unintentional weight loss associated with poor prognosis. Exosomes, originating from multivesicular bodies, carry biomolecular cargo that can alter cell signaling. They affect muscle and adipose tissue metabolism, macrophage polarization, and apoptosis, contributing to cachexia phenotype. Tumor-derived exosomes have potential as biomarkers and play a role in cancer cachexia progression by affecting skeletal muscle and adipose tissue.
Review
Biochemistry & Molecular Biology
Sarah A. Walsh, Benjamin W. Hoyt, Cassie J. Rowe, Devaveena Dey, Thomas A. Davis
Summary: Severe polytraumatic injury can lead to immune dysfunction, but early biomarkers for immune dysfunction are currently lacking. Study of exosomal cargo may provide critical insights for intervention.
Review
Cell Biology
Sarah Beck, Bernhard Hochreiter, Johannes A. Schmid
Summary: Extracellular vesicles (EVs) play crucial roles in intercellular communication by transferring nucleotides and proteins, and are associated with diseases such as cancer, inflammation, and thrombosis. They have the potential to serve as biomarkers for diagnosis and as vehicles for therapeutics in the future.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lee Ohayon, Xinyi Zhang, Partha Dutta
Summary: Extracellular vesicles, including exosomes and micro-vesicles, play a significant role in cardiovascular diseases by transporting bioactive molecules and impacting target cells and tissues through autocrine and paracrine signaling. They can have both beneficial and detrimental effects on inflammation processes in cardiovascular diseases.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Cell Biology
Xingyi Chen, Baolong Liu, Xingzhi Li, Thuy T. An, You Zhou, Gang Li, Judy Wu-Smart, Sophie Alvarez, Michael J. Naldrett, James Eudy, Gregory Kubik, Richard A. Wilson, Stephen D. Kachman, Juan Cui, Jiujiu Yu
Summary: Honey has been used as a traditional medicinal food and modern research has discovered its anti-inflammatory properties, as well as a new bioactive component that can alleviate inflammation and liver damage.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Geriatrics & Gerontology
Xiaohui Zhang, Yanxia Zhao, Wei Yan
Summary: Skeletal muscle wasting is a common condition accompanied by various diseases, significantly affecting patients' quality of life and prognosis. Recent studies have revealed the important role of extracellular vesicles (EVs) in the progression of muscle wasting, as they can transfer bioactive cargoes to skeletal muscle. However, the role of EVs in muscle wasting is highly dependent on their parental cells and the specific type of cargo they carry.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Microbiology
Risa Imamiya, Akari Shinohara, Daisuke Yakura, Takehiro Yamaguchi, Koji Ueda, Ami Oguro, Yukiko Minamiyama, Hiroshi Ichikawa, Yasuhiko Horiguchi, Mayuko Osada-Oka
Summary: Extracellular vesicles (EVs) released from macrophages infected with live Escherichia coli can induce inflammatory responses by uninfected macrophages. The inflammatory responses induced by EVs from macrophages infected with heat-inactivated E. coli or lipopolysaccharide are weaker compared to those induced by outer membrane vesicles (OMVs) from live E. coli. The proteins OmpA, GroL1, DegP, CirA, and FepA from E. coli are potential triggers of exosome-mediated inflammatory responses.
Article
Cell Biology
Kiel A. Peck, Alessandra Ciullo, Liang Li, Chang Li, Ashley Morris, Eduardo Marban, Ahmed Gamal Ibrahim
Summary: The study reveals that the target gene TDO2 of canonical Wnt signaling plays a crucial role in enhancing the therapeutic potency of cells and their EVs, improving disease conditions by immunomodulation and reducing inflammation response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Cell Biology
Tiantian Li, Hongchi Yu, Demao Zhang, Tang Feng, Michael Miao, Jianwei Li, Xiaoheng Liu
Summary: Vascular calcification is associated with increased risk of heart disease, stroke, and atherosclerotic plaque rupture. Extracellular vesicles, particularly matrix vesicles, play a crucial role in the early stages of vascular calcification. However, the underlying mechanism by which matrix vesicles drive vascular smooth muscle cells phenotype switching and calcification remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Valentina Carotti, Juan P. Rigalli, Jenny van Asbeck-van der Wijst, Joost G. J. Hoenderop
Summary: Purinergic signalling is a process mediated by purinergic receptors, where extracellular nucleotides and nucleosides bind to the receptors, resulting in changes in cell physiology. This signalling is involved in important physiological processes and pathophysiological situations such as cancer and inflammation. Extracellular vesicles (EVs) play a crucial role in modulating purinergic signalling and can affect target cells and tissues located at distant locations. The regulation of extracellular ATP levels and adenosine by EVs has significant impact on processes like inflammation, immune response, and cancer pathogenesis.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Tian Tian, Lei Cao, Chuan He, Qing Ye, Ruyu Liang, Weiyan You, Huixin Zhang, Jiahuan Wu, Jinhai Ye, Bakhos A. Tannous, Jun Gao
Summary: Ischemic stroke is a major cause of death and anti-inflammatory strategies are promising for reducing brain injury during reperfusion. Stem cell-derived extracellular vesicles (EVs) have emerged as therapeutic agents for immune modulation. By attaching targeting ligands onto EVs using a recombinant fusion protein, researchers were able to deliver anti-inflammatory EVs to the ischemic brain, showing promising results in suppressing inflammation.
Article
Engineering, Biomedical
Saigopalakrishna S. Yerneni, Ezgi P. Yalcintas, Jason D. Smith, Saadyah Averick, Phil G. Campbell, O. Burak Ozdoganlar
Summary: This study introduces and evaluates a skin-targeted delivery system for curcumin that combines albumin association, extracellular vesicle (EV) encapsulation, and dissolvable microneedle arrays (dMNAs). In vitro experiments demonstrate that curcumin-albumin encapsulated EVs (CA-EVs) can inhibit and reverse the expression of inflammatory transcription factor NF-.B triggered by lipopolysaccharide (LPS). The integration of CA-EVs into dMNAs does not affect their physical or functional properties. Importantly, dMNAs extend the storage stability of EVs for at least 12 months at room temperature with minimal loss in their bioactivity. We demonstrate that dMNA delivered CA-EVs effectively block and reverse skin inflammation in vivo in mouse and rat models.
ACTA BIOMATERIALIA
(2022)
Review
Oncology
Layla Simon, Sofia Sanhueza, Belen Gaete-Ramirez, Manuel Varas-Godoy, Andrew F. G. Quest
Summary: Advances in cancer biology have led to different treatments for improving patients' survival rates. However, relapse and recurrence remain common due to therapy resistance in residual cancer cells, which is influenced by the inflammatory tumor microenvironment (TME) and extracellular vesicles (EVs). Inflammatory conditions alter the release and composition of EVs within the TME, affecting tumor cells' response to cancer therapies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Engineering, Biomedical
Jeremy P. Bost, Osama Saher, Daniel Hagey, Doste R. Mamand, Xiuming Liang, Wenyi Zheng, Giulia Corso, Oskar Gustafsson, Andre Gorgens, Ci Edvard Smith, Rula Zain, Samir El Andaloussi, Dhanu Gupta
Summary: Extracellular vesicles (EVs) are nanosized cell-derived vesicles that facilitate intercellular communication via transmitting biological cargo. The production of EVs is influenced by various pathways and external factors in the cell media, with ceramide-dependent pathway being a major contributor to engineered EV production. The study provides insights into understanding and optimizing EV production in cell culture for developing advanced methods.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biology
Jeremy P. Bost, Miina Ojansivu, Michael J. Munson, Emelie Wesen, Audrey Gallud, Dhanu Gupta, Oskar Gustafsson, Osama Saher, Julia Radler, Stuart G. Higgins, Taavi Lehto, Margaret N. Holme, Anders Dahlen, Ola Engkvist, Per-Erik Stromstedt, Shalini Andersson, C. I. Edvard Smith, Molly M. Stevens, Elin K. Esbjorner, Anna Collen, Samir El Andaloussi
Summary: By interfering with the trafficking of SSOs to LAMP1-positive endosomal compartments, inducing endosomal membrane rupture and facilitating the escape of SSOs into the cytosol, these two novel endosomolytic compounds have shown increased delivery efficacy of splice-switching oligonucleotides.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Xiuming Liang, Zheyu Niu, Valentina Galli, Nathalie Howe, Ying Zhao, Oscar P. B. Wiklander, Wenyi Zheng, Rim Jawad Wiklander, Giulia Corso, Christopher Davies, Justin Hean, Eleni Kyriakopoulou, Doste R. Mamand, Risul Amin, Joel Z. Nordin, Dhanu Gupta, Samir E. L. Andaloussi
Summary: This study explores an engineering strategy to prolong the circulation time of extracellular vesicles (EVs) by decorating the surface with albumin. The engineered EVs demonstrate strong binding capacity and significantly increased circulation time after injection in mice. Furthermore, they accumulate in lymph nodes and solid tumors, which can be utilized for immunomodulation, cancer therapy, and immunotherapy.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Hematology
Daniel W. Hagey, Egle Kvedaraite, Mira Akber, Andre Goergens, Joman Javadi, Tatiana von Bahr Greenwood, Caroline Bjorklund, Selma Olsson Akefeldt, Tova Hannegard-Hamrin, Henrik Arnell, Katalin Dobra, Nikolas Herold, Mattias Svensson, Samir El Andaloussi, Jan-Inge Henter, Magda Lourda
Summary: This study compared monocyte-derived dendritic cells from LCH patients to those from healthy controls or patients with Crohn's disease. The findings showed that LCH cells exhibited increased endo- and exocytosis gene activity and the enrichment of pathological transcripts in extracellular vesicles. The study also indicated that the LCH secretome had a significant effect on lymphocyte activity.
Article
Biology
Valeria Taliani, Giulia Buonaiuto, Fabio Desideri, Adriano Setti, Tiziana Santini, Silvia Galfre, Leonardo Schirone, Davide Mariani, Giacomo Frati, Valentina Valenti, Sebastiano Sciarretta, Emerald Perlas, Carmine Nicoletti, Antonio Musaro, Monica Ballarino
Summary: Long noncoding RNAs (lncRNAs) play a critical role in heart physiology and disease. We identified a lncRNA called pCharme that is associated with chromatin and is involved in myogenesis and cardiac muscle remodeling in mice. Through various molecular analyses, we found that pCharme is specifically expressed in cardiomyocytes and is important for their maturation and cardiac development. The loss of pCharme leads to delayed cardiomyocyte maturation and morphological alterations in the ventricular myocardium. This study provides valuable insights into a novel lncRNA-mediated regulatory mechanism in heart development and has implications for future theranostic applications targeting the Charme locus.
Review
Cell Biology
Joshua A. Welsh, Ger J. A. Arkesteijn, Michel Bremer, Michael Cimorelli, Francoise Dignat-George, Bernd Giebel, Andre Goergens, An Hendrix, Martine Kuiper, Romaric Lacroix, Joanne Lannigan, Ton G. van Leeuwen, Estefania Lozano-Andres, Shoaib Rao, Stephane Robert, Leonie de Rond, Vera A. Tang, Tobias Tertel, Xiaomei Yan, Marca H. M. Wauben, John P. Nolan, Jennifer C. Jones, Rienk Nieuwland, Edwin van der Pol
Summary: Flow cytometry (FCM) is able to characterize single extracellular vesicles (EVs), but EVs are more difficult to detect than cells because their signals overlap with background noise. To overcome this issue, the EV FCM working group published a framework (MIFlowCyt-EV) to improve the interpretation, reporting, and reproducibility of EV FCM experiments.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Chemistry, Multidisciplinary
Wei Jun Chen, Dhanu Gupta, Meiyang Yang, Fuwei Yang, Ning Feng, Junling Song, Matthew J. A. Wood, Lipeng Qiu, Jinghua Chen
Summary: Metal-organic frameworks (MOFs) with tunable porosity, biocompatibility, and modularity have shown promise as novel therapeutics for malignancies. However, the design and synthesis of dual-stimuli responsive MOFs for controlled drug release in tumor microenvironments are challenging. This study synthesized pH/glutathione (GSH)-sensitive MnFe-based MOFs combined with gold nanoparticles (Au NPs) and cisplatin prodrugs (DSCP) for chemo-chemodynamic-starvation synergistic therapy.
Article
Multidisciplinary Sciences
Wenyi Zheng, Julia Raedler, Helena Sork, Zheyu Niu, Samantha Roudi, Jeremy P. P. Bost, Andre Goergens, Ying Zhao, Doste R. R. Mamand, Xiuming Liang, Oscar P. B. Wiklander, Taavi Lehto, Dhanu Gupta, Joel Z. Z. Nordin, Samir EL Andaloussi
Summary: This study conducted a large-scale screening to identify potential scaffold proteins for loading therapeutic cargo into extracellular vesicles (EVs). They found 24 proteins with conserved EV-sorting abilities, including TSPAN2 and TSPAN3 which outperformed the well-studied CD63 scaffold. These engineered EVs showed promise as delivery vehicles in cell cultures and mice.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Daniel W. Hagey, Miina Ojansivu, Beklem R. Bostancioglu, Osama Saher, Jeremy P. Bost, Manuela O. Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M. Stevens, Andre Goergens, Samir EL Andaloussi
Summary: This study provides important insights into the cellular response to extracellular vesicles (EVs), showing that EV dose has a more significant effect than cell source. At high doses, exocytosis is down-regulated and lysosomal activity is up-regulated. However, at low doses, specific responses based on EV cell source are observed, reflecting the activities of the EV's source cells. Furthermore, immune cell-derived EVs are most associated with recipient cells.
Article
Cell Biology
Chen Li, Chengxi Sun, Warangkana Lohcharoenkal, Mohamad Moustafa Ali, Pengwei Xing, Wenyi Zheng, Andre Gorgens, Manuela O. Gustafsson, Samir EL Andaloussi, Eniko Sonkoly, Andor Pivarcsi
Summary: Cutaneous squamous cell carcinoma (cSCC) cells secrete a higher number of extracellular vesicles (EVs) compared to normal human epidermal keratinocytes (NHEKs). Inhibiting the secretion of EVs from cSCC cells can suppress tumor growth in a xenograft model. EVs derived from metastatic cSCC cells induce the conversion of fibroblasts into cancer-associated fibroblasts (CAFs).
CELL DEATH DISCOVERY
(2023)
Article
Cell Biology
Simon Heidegger, Florian Stritzke, Sarah Dahl, Juliane Dassler-Plenker, Laura Joachim, Dominik Buschmann, Kaiji Fan, Carolin M. Sauer, Nils Ludwig, Christof Winter, Stefan Enssle, Suqi Li, Markus Perl, Andre Goergens, Tobias Haas, Erik Thiele Orberg, Sascha Goettert, Catherine Woelfel, Thomas Engleitner, Isidro Cortes-Ciriano, Roland Rad, Wolfgang Herr, Bernd Giebel, Jurgen Ruland, Florian Bassermann, Christoph Coch, Gunther Hartmann, Hendrik Poeck
Summary: Tumor-derived extracellular vesicles (EVs) play a role in immune evasion and tumor progression through the RNA-sensing receptor RIG-I. Activation of RIG-I releases EVs that promote dendritic cell maturation and T cell antitumor immunity, synergizing with immune checkpoint blockade. Intact RIG-I, autocrine interferon signaling, and the GTPase Rab27a are necessary for the generation of immunostimulatory EVs. The composition of the EV RNA cargo, including small non-coding stimulatory RNAs, is regulated by active intrinsic RIG-I signaling. Increased transcriptional activity of EV pathway genes and RIG-I in melanoma samples is associated with prolonged patient survival and positive response to immunotherapy. EVs generated from human melanoma after RIG-I stimulation induce potent antigen-specific T cell responses.
CELL REPORTS MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Lin Li, Veronika Mussack, Andre Goergens, Elena Pepeldjiyska, Anne Sophie Hartz, Hazal Aslan, Elias Rackl, Andreas Rank, Joerg Schmohl, Samir El Andaloussi, Michael W. Pfaffl, Helga Schmetzer
Summary: This study demonstrates the potential of extracellular vesicle-derived microRNA profiles as biomarkers to distinguish between acute myeloid leukemia and healthy samples.
NANOSCALE ADVANCES
(2023)
Meeting Abstract
Dermatology
C. Li, C. Sun, J. J. Elton, W. Lohcharoenkal, A. Gorgens, S. El Andaloussi, E. Sonkoly, A. Pivarcsi
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
