Marine biomimetics: bromotyrosines loaded chitinous skeleton as source of antibacterial agents

The marine sponges of the order Verongiida (Demospongiae: Porifera) have survived on our planet for more than 500 million years due to the presence of a unique strategy of chemical protection by biosynthesis of more than 300 derivatives of biologically active bromotyrosines as secondary metabolites. These compounds are synthesized within spherulocytes, highly specialized cells located within chitinous skeletal fibers of these sponges from where they can be extruded in the sea water and form protective space against pathogenic viruses, bacteria and other predators. This chitin is an example of unique biomaterial as source of substances with antibiotic properties. Traditionally, the attention of researchers was exclusively drawn to lipophilic bromotyrosines, the extraction methods of which were based on the use of organic solvents only. Alternatively, we have used in this work a biomimetic water-based approach, because in natural conditions, sponges actively extrude bromotyrosines that are miscible with the watery environment. This allowed us to isolate 3,5-dibromoquinolacetic acid from an aqueous extract of the dried demosponge Aplysina aerophoba and compare its antimicrobial activity with the same compound obtained by the chemical synthesis. Both synthetic and natural compounds have shown antimicrobial properties against clinical strains of Staphylococcus aureus, Enterococcus faecalis and Propionibacterium acnes.

Automated summary

Marine sponges of the order Verongiida have survived for more than 500 million years by utilizing a unique chemical protection strategy through biosynthesis of over 300 biologically active bromotyrosine derivatives. These compounds are synthesized within specialized cells and can be extruded into seawater to form a protective space against pathogens. The compounds show antimicrobial activity against clinical strains of various bacteria, and the research has shifted towards a water-based extraction method to mimic natural conditions for isolating these compounds.