Article
Rheumatology
Theresa Graalmann, Katharina Borst, Himanshu Manchanda, Lea Vaas, Matthias Bruhn, Lukas Graalmann, Mario Koster, Murielle Verboom, Michael Hallensleben, Carlos Alberto Guzman, Gerd Sutter, Reinhold E. Schmidt, Torsten Witte, Ulrich Kalinke
Summary: Rituximab-treated patients and B cell-deficient mice showed reduced expansion of virus-specific CD8(+) T cells after vaccination/infection with different vaccines/pathogens, indicating that B cells can modulate CD8(+) T cell responses. The choice of vaccines for B cell-depleted patients needs to be re-evaluated to effectively induce protective CD8(+) T cell responses.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Gastroenterology & Hepatology
Benjamin G. Wiggins, Laura J. Pallett, Xiaoyan Li, Scott P. Davies, Oliver E. Amin, Upkar S. Gill, Stephanie Kucykowicz, Arzoo M. Patel, Konstantinos Aliazis, Yuxin S. Liu, Gary M. Reynolds, Brian R. Davidson, Amir Gander, Tu Vinh Luong, Gideon M. Hirschfield, Patrick T. F. Kennedy, Yuehua Huang, Mala K. Maini, Zania Stamataki
Summary: This study found that high and intermediate CD69 expressions marked human hepatic CD4(+) T-RM and a novel functionally distinct recirculating population, both shaped by the liver microenvironment to achieve diverse immunosurveillance.
Article
Immunology
Constantina A. Bounia, Stamatis-Nick C. Liossis
Summary: Treatment with rituximab (RTX) significantly reduces circulating Th17 cell percentages in patients with RA, while Tregs remain stable. Reduction of Th17 cells is particularly evident in responders to RTX treatment, ACPA+ and RF (-) patients with RA.
CLINICAL IMMUNOLOGY
(2021)
Editorial Material
Oncology
Winnie Yao, Ansuman T. Satpathy
Summary: Transient depletion of CD4(+) T cells in patients with gastrointestinal cancer induces remodeling of the T-cell repertoire, including clonal replacement and expansion of CD8(+) T-cell clones shared between the blood and tumor, deepening our understanding of tumor-specific CD8(+) T-cell responses.
CANCER IMMUNOLOGY RESEARCH
(2021)
Review
Immunology
Livius Penter, Satyen H. Gohil, Catherine J. Wu
Summary: Blood malignancies offer unique opportunities for studying disease evolution and monitoring anti-tumor immune changes. The development of single-cell sequencing technologies allows us to delve into the underlying mechanisms at an unprecedented level of detail. The identification of molecular events as in-vivo barcodes enables tracking of malignant and immune cell populations over time.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biophysics
Lin Wang, Yu Zhuang, Yue Yu, Zhiwei Guo, Qiaomei Guo, Lihua Qiao, Xueqing Wang, Xiaohui Liang, Pengpeng Zhang, Qifan Li, Chenjun Huang, Rong Cong, Yinghui Li, Bin Che, Huihui Xiong, Guomin Lin, Mingming Rao, Rongjun Hu, Wei Wang, Guohua Yang, Jiatao Lou
Summary: We developed the OPERA system to detect mutations with low variant allelic frequency (VAF) in short DNA fragments. OPERA can detect mutations as low as 0.0025% VAF, while CAPP-Seq can only detect mutations >0.03% VAF. OPERA has high sensitivity in detecting mutations in synthetic fragments and plasma samples from lung cancer patients.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Infectious Diseases
Margaux Garzaro, Lin-Pierre Zhao, Nathalie De Castro, Severine Mercier-Delarue, Francois Camelena, Sabine Pereyre, Marie Gardette, Beatrice Bercot, Marion Malphettes, Cecile Bebear, Jean-David Bouaziz, Jerome Le Goff, Lionel Galicier, Maud Salmona
Summary: This is a case report of a 45-year-old female patient receiving rituximab for B cell non-Hodgkin follicular lymphoma, who presented with unexplained recurrent fever, abdominal discomfort, and pollakiuria. Shotgun metagenomic sequencing from peri-kidney collection identified a co-infection with Mycoplasma hominis and Ureaplasma urealyticum. The patient recovered with sequelae after appropriate antibiotic treatment was given.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Oncology
Hager Mohamed, Eric Gebski, Rufranshell Reyes, Samuel Beane, Brian Wigdahl, Fred C. Krebs, Katharina Stapelmann, Vandana Miller
Summary: The study examined the responses of two human leukemic cell lines to NTP-generated reactive oxygen and nitrogen species, demonstrating that cells can modulate the RONS-mediated changes in liquid chemistry, and the immunomodulatory effects of NTP may be independent of its cytotoxicity.
Article
Biochemistry & Molecular Biology
Ni Zeng, Christophe M. Capelle, Alexandre Baron, Takumi Kobayashi, Severine Cire, Vera Tslaf, Cathy Leonard, Djalil Coowar, Haruhiko Koseki, Astrid M. Westendorf, Jan Buer, Dirk Brenner, Rejko Krueger, Rudi Balling, Markus Ollert, Feng Q. Hefeng
Summary: Loss of DJ-1 gene reduces signs of immune aging, providing a potential target for intervention in immune aging and aging-related diseases.
Article
Oncology
Marie Bleakley, Alison Sehgal, Stuart Seropian, Melinda A. Biernacki, Elizabeth F. Krakow, Ann Dahlberg, Heather Persinger, Barbara Hilzinger, Paul J. Martin, Paul A. Carpenter, Mary E. Flowers, Jenna Voutsinas, Theodore A. Gooley, Keith Loeb, Brent L. Wood, Shelly Heimfeld, Stanley R. Riddell, Warren D. Shlomchik
Summary: This study evaluated the impact of T-N depletion on chronic GVHD (cGVHD) and other outcomes. The results showed that T-N depletion significantly reduced the incidence of GVHD without increasing the risk of relapse and nonrelapse mortality.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Cell Biology
Mariana O. Diniz, Anna Schurich, Senthil K. Chinnakannan, Marion Duriez, Kerstin A. Stegmann, Jessica Davies, Stephanie Kucykowicz, Kornelija Suveizdyte, Oliver E. Amin, Frances Alcock, Tamsin Cargill, Eleanor Barnes, Mala K. Maini
Summary: This study demonstrates that NK cell depletion enhances the immune response to vaccines in a mouse model of chronic HBV infection. It was found that the up-regulation of PD-L1 on liver-resident NK cells and PD-1 on intrahepatic T cells plays a negative regulatory role in the response to therapeutic vaccination. Combining cytokine activation with PD-L1 blockade can convert NK cells into efficient helpers, boosting the CD8(+) T cell response to therapeutic vaccination.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Analytical
Sisi Zhang, Bo Zhao, Stephanie Adaniya, Hui Xiao, Ning Li
Summary: This study demonstrates a novel strategy to reliably quantify trace amounts of host cell proteins (HCPs) in biotherapeutic drugs, achieving detection limits as low as 0.06 ppm and good accuracy and precision. The results show that certain HCPs can affect the stability of drug products, while others can be safely present. Accurate quantification of lipases/esterases in drug products, combined with lipase activity analysis, provides a better understanding of enzymatic degradation from endogenous proteins.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Pearl A. Sutter, Antoine Menoret, Evan R. Jellison, Alexandra M. Nicaise, Allison M. Bradbury, Anthony T. Vella, Ernesto R. Bongarzone, Stephen J. Crocker
Summary: Globoid cell leukodystrophy (GLD), or Krabbe's disease, is a fatal genetic demyelinating disease caused by loss-of-function mutations in the galactosylceramidase (galc) gene. A study conducted on a mouse model of GLD has identified CD8(+) cytotoxic T lymphocytes as a key player in the development of the disease and its neuropathology. Blocking CD8 alpha effectively prevented disease onset, reduced morbidity and mortality, and prevented CNS demyelination in mice, suggesting potential novel therapeutic targets for GLD.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Marit J. van Elsas, Johan M. S. van der Schoot, Alexander Bartels, Kas Steuten, Duco van Dalen, Zacharias Wijfjes, Carl G. Figdor, Thorbald van Hall, Sjoerd H. van der Burg, Martijn Verdoes, Ferenc A. Scheeren
Summary: Regulatory T cells play a crucial role in immune suppression and pose challenges in cancer therapy. By optimizing the Fc domain of an antibody, efficient depletion of tumor-resident regulatory T cells can be achieved. Using a genome engineering strategy, a stable cell line producing optimized antibodies was generated, leading to effective depletion of tumor-resident regulatory T cells and enhanced tumor eradication when combined with other antibodies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Christoph Helmstaedter, Niels Hansen, Pitshaporn Leelaarporn, Kerstin Schwing, Demet Oender, Guido Widman, Attila Racz, Rainer Surges, Albert Becker, Juri-Alexander Witt
Summary: The study found a link between neuropsychological impairments in autoimmune limbic encephalitis (LE) patients and T- and B-cell activity, especially in patients with autoantibodies against intracellular antigens.
JOURNAL OF NEUROLOGY
(2021)
Review
Nanoscience & Nanotechnology
Zhenpeng Yao, Yanwei Lum, Andrew Johnston, Luis Martin Mejia-Mendoza, Xin Zhou, Yonggang Wen, Alan Aspuru-Guzik, Edward H. Sargent, Zhi Wei Seh
Summary: This Perspective highlights the recent advances in machine learning-driven energy research and proposes a set of key performance indicators to compare the benefits of different ML-accelerated workflows in the field of renewable energy.
NATURE REVIEWS MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Ning Wang, Pengfei Ou, Sung-Fu Hung, Jianan Erick Huang, Adnan Ozden, Jehad Abed, Ivan Grigioni, Clark Chen, Rui Kai Miao, Yu Yan, Jinqiang Zhang, Ziyun Wang, Roham Dorakhan, Ahmed Badreldin, Ahmed Abdel-Wahab, David Sinton, Yongchang Liu, Hongyan Liang, Edward H. Sargent
Summary: Direct electrolysis of pH-neutral seawater to generate hydrogen faces an anodic competition issue between the chlorine evolution and the oxygen evolution reaction (OER), resulting in low current density and limited operating stability. In this study, a proton-adsorption-promoting strategy has been proposed to increase the OER rate, allowing for enhanced and stable neutral seawater splitting. Palladium-doped cobalt oxide (Co3-xPdxO4) catalysts exhibit the best performance with an OER overpotential of 370 mV at 10 mA cm(-2) in pH-neutral simulated seawater, outperforming Co3O4 by 70 mV. Co3-xPdxO4 catalysts also demonstrate long-term stability in neutral seawater, with 450 hours at 200 mA cm(-2) and 20 hours at 1 A cm(-2). Experimental and theoretical analyses suggest that the inclusion of SPA cations accelerates the rate-determining water dissociation step in the neutral OER pathway, while ruling out additional OER sites as a main factor.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Physical
Haoyue Wan, Fengyan Jia, Filip Dinic, Muhammad Imran, Benjamin Rehl, Yanjiang Liu, Watcharaphol Paritmongkol, Pan Xia, Ya-Kun Do Wang, Yuan Liu, Sasa Wang, Quan Lyu, Giovanni Francesco Cotella, Peter Chun, Oleksandr Voznyy, Sjoerd Hoogland, Edward H. Sargent
Summary: Lead-free double perovskites demonstrate non-toxic properties and strong emission by incorporating alloying and doping. In this study, Bidoped pure Rb2HfCl6 and Cs-alloyed RbCsHfCl6 microcrystals were synthesized. The pure Rb2HfCl6 microcrystals showed minimal emission, while alloying with Cs and doping with Bi resulted in strong deep blue emission at 466 nm with an increased photoluminescence quantum yield from approximately 0 to 66%. Density functional theory analysis confirmed the enhanced phase stability due to Cs-alloying and linked the photoluminescence to localized states induced by Bi3+ dopants.
CHEMISTRY OF MATERIALS
(2023)
Article
Energy & Fuels
Adnan Ozden, Jun Li, Sharath Kandambeth, Xiao-Yan Li, Shijie Liu, Osama Shekhah, Pengfei Ou, Y. Zou Finfrock, Ya-Kun Wang, Tartela Alkayyali, F. Pelayo Garcia de Arquer, Vinayak S. Kale, Prashant M. Bhatt, Alexander H. Ip, Mohamed Eddaoudi, Edward H. Sargent, David Sinton
Summary: Carbon dioxide/monoxide (CO2/CO) electrolysis has the potential to convert emissions into multicarbon products, but current systems suffer from low energy and carbon efficiencies. In this study, the researchers identified uncontrolled gas/ion distributions as the source of these inefficiencies and developed a strategy to block cation migration to the catalyst surface using a covalent organic framework (COF). The COF-mediated catalyst achieved a single-pass carbon efficiency of 95%, an energy efficiency of 40%, and a current density of 240 mA cm(-2) during 200 hours of electrosynthesis of multicarbon products from CO.
Article
Chemistry, Multidisciplinary
Alam Mahmud, Dingran Chang, Jagotamoy Das, Surath Gomis, Farid Foroutan, Jenise B. Chen, Laxman Pandey, Connor D. Flynn, Hanie Yousefi, Armin Geraili, Heather J. Ross, Edward H. Sargent, Shana O. Kelley
Summary: This study presents a reagent-free electronic sensing system that can analyze disease markers directly in unprocessed body fluids, paving the way for simple and affordable devices for personalized healthcare monitoring.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Zongjie Wang, Hansen Wang, Sichun Lin, Mahmoud Labib, Sharif Ahmed, Jagotamoy Das, Stephane Angers, Edward H. Sargent, Shana O. Kelley
Summary: Nanoneedles are effective in delivering biomolecules to cells, but the mechanisms underlying cell-nanoneedle interactions are not well understood. In this study, we developed a new method to generate nanoneedles, confirmed their cargo delivery capability, and investigated the genetic factors involved in the process. Our results showed that the nanoneedles disrupted cell membranes, increased the expression of cell-cell junction proteins, and downregulated transcriptional factors of the NF kappa B pathway. This disturbance caused most cells to be trapped in the G2 phase, where endocytosis activities are highest. This system provides a new model for studying cell interactions with high-aspect-ratio materials.
Article
Multidisciplinary Sciences
Chongwen Li, Xiaoming Wang, Enbing Bi, Fangyuan Jiang, So Min Park, You Li, Lei Chen, Zaiwei Wang, Lewei Zeng, Hao Chen, Yanjiang Liu, Corey R. Grice, Abasi Abudulimu, Jaehoon Chung, Yeming Xian, Tao Zhu, Huagui Lai, Bin Chen, Randy J. Ellingson, Fan Fu, David S. Ginger, Zhaoning Song, Edward H. Sargent, Yanfa Yan
Summary: Through density functional theory calculations and experimental tests, it was found that phosphine-containing molecules, including 1,3-bis(diphenylphosphino)propane (DPPP), can strongly bind and passivate undercoordinated lead atoms at interfaces and grain boundaries in metal halide perovskite solar cells. This binding leads to enhanced durability and long-term stability, as demonstrated by the PSCs retaining their high power conversion efficiency (PCE) even after extended operation or exposure to extreme conditions.
Article
Chemistry, Multidisciplinary
Asif Abdullah Khan, Md Masud Rana, Sasa Wang, Md Fahim Al Fattah, Muhammed Kayaharman, Kaiping Zhang, Shawn Benedict, I. A. Goldthorpe, Y. Norman Zhou, Edward H. Sargent, Dayan Ban
Summary: Regulating the strain and halogen substitution in inorganic perovskite structures has led to the discovery of piezoelectric materials with high piezoelectric coefficients. The Iodine-incorporated CsPbBr2I exhibits the highest intrinsic piezoelectric response among all inorganic metal halide perovskites. The high piezoelectricity is attributed to halogen-induced lattice strain and octahedral distortion. This study opens up a new avenue for designing piezoelectric materials and paves the way for the development of efficient piezoelectric energy converters.
Article
Multidisciplinary Sciences
Sasa Wang, Asif Abdullah Khan, Sam Teale, Jian Xu, Darshan H. Parmar, Ruyan Zhao, Luke Grater, Peter Serles, Yu Zou, Tobin Filleter, Dwight S. Seferos, Dayan Ban, Edward H. Sargent
Summary: Piezoelectric materials are essential for self-powered electronics and energy harvesting. However, existing piezoelectrics have limitations in terms of charge and voltage coefficients. In this study, researchers developed a molecular piezoelectric material with enhanced properties by utilizing quasi-spherical theory and Jahn-Teller distortion. This new material achieved high power density in piezoelectric energy harvesters.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Luke Grater, Mingcong Wang, Sam Teale, Suhas Mahesh, Aidan Maxwell, Yanjiang Liu, So Min Park, Bin Chen, Freideiric Laquai, Mercouri G. Kanatzidis, Edward H. Sargent
Summary: Band gap tuning in mixed-halide perovskites enables efficient multijunction solar cells and LEDs. However, wide band gap perovskites phase segregate under illumination, introducing voltage losses that limit stability. This study explores the role of halide vacancies in anion migration to erect local barriers to ion migration, using a 3D hollow perovskite structure with ethane-1,2-diammonium dihydroiodide (EDA) as the hollowing agent. Results show that 1% EDA can stabilize a 40% bromine mixed-halide perovskite at 1 sun illumination intensity, suggesting that hollow sites limit the mobility of the halide vacancies.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Ping Fu, Michael A. Quintero, Eugenia S. Vasileiadou, Parth Raval, Claire Welton, Mikael Kepenekian, George Volonakis, Jacky Even, Yukun Liu, Christos Malliakas, Yi Yang, Craig Laing, Vinayak P. Dravid, G. N. Manjunatha Reddy, Can Li, Edward H. Sargent, Mercouri G. Kanatzidis
Summary: This study investigates two-dimensional alloyed Pb/Sn bromide perovskites in the Ruddlesden-Popper (RP) and Dion-Jacobson (DJ) phases using butylammonium and 3-(aminomethyl)pyridinium as spacer cations. The results show that the ratio and site preference of Pb/Sn atoms are influenced by layer thickness and spacer cations. Pb-rich alloys are thermodynamically favored in n = 1 compounds. Films in the RP phase exhibit parallel orientation to the substrate, while random orientations are observed for DJ cases.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biotechnology & Applied Microbiology
Zongjie Wang
Summary: Stem cells have the potential to proliferate and differentiate into various cell types, making them promising in regenerative medicine. However, concerns about their tumorigenicity require the assessment of safety in stem cell-derived therapies. Various approaches, including animal models, culture methods, and molecular techniques, can be used to assess tumorigenicity, each with its own advantages and limitations. Combining multiple assays may provide a more comprehensive evaluation. Future developments should focus on optimizing microfluidics-based methods and integrating multiple assays into a single platform for efficient and comprehensive tumorigenicity evaluation.
BIOENGINEERING-BASEL
(2023)
Article
Biochemical Research Methods
Kangfu Chen, Zongjie Wang
Summary: Advancements in single-cell proteomics have opened new possibilities for analyzing rare cells. Efficient sample preparation is crucial for the success of single-cell proteomics, and this study proposes a microfluidics-based method for highly efficient isolation, detection, and collection of rare cells such as CTCs, providing an effective handling system for subsequent proteomic analysis.
METHODS AND PROTOCOLS
(2023)
Article
Biochemistry & Molecular Biology
Tanja Sack, Piriththiv Dhavarasa, Daniel Szames, Siobhan O'Brien, Stephane Angers, Shana O. Kelley
Summary: Comparing to nuclear DNA repair mechanisms, the characterization of mtDNA repair mechanisms is difficult due to the challenge of specifically addressing mitochondrial damage. This study uses an mtDNA-damaging agent in combination with CRISPR/Cas9 screening to identify genes essential for mtDNA damage response and confirms the responsiveness of WRNIP1 to mtDNA damage for the first time. The study also reveals the mitochondrial role of WRNIP1 in innate immune signaling and nuclear genome maintenance.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Zongjie Wang, Dingran Chang, Edward H. Sargent, Shana O. Kelley
Summary: This article introduces an Apta FastZ algorithm that can accelerate the Pro-SELEX workflow by achieving a computing speed of 10 to 40 times faster compared to the original AptaZ algorithm while maintaining identical outcomes. The affinity of myeloperoxidase aptamers predicted by Apta FastZ was validated by experiments, showing a high level of linear correlation with measured affinities.
ANALYTICAL CHEMISTRY
(2023)