Article
Chemistry, Analytical
Shiliang Chen, Liuwei Meng, Litong Wang, Xixi Huang, Shujat Ali, Xiaojing Chen, Mingen Yu, Ming Yi, Limin Li, Xi Chen, Leiming Yuan, Wen Shi, Guangzao Huang
Summary: The newly developed SERS-based LFIA strip using GERTs achieved highly sensitive detection of anti-SARS-CoV-2 IgM and IgG, with detection limits 100 times lower compared to conventional LFIA strips, improving the accuracy and reliability of early diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Sung Eun Seo, Eunsu Ryu, Jinyeong Kim, Chan Jae Shin, Oh Seok Kwon
Summary: The rapid and sensitive detection of SARS-CoV-2 has become a priority for public health. In this study, a new method was proposed to improve the performance of the lateral flow assay sensor by concentrating fluorescent organic molecules. This approach showed high sensitivity, fast detection, and outperformed commercial test kits in clinical tests.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Chunhua Wang, Chao Wang, Jiaoyan Qiu, Jianwei Gao, Hong Liu, Yu Zhang, Lin Han
Summary: The space-encoding microfluidic biochip developed in this study offers a fast, sensitive method for detecting the SARS-CoV-2 virus, with high throughput, small sample volumes, quick detection, linear quantification, and ultra-low detection limit.
Article
Chemistry, Analytical
Haifeng Liu, Erhei Dai, Rui Xiao, Zihui Zhou, Minli Zhang, Zikun Bai, Ying Shao, Kezong Qi, Jian Tu, Chongwen Wang, Shengqi Wang
Summary: This study reported a surface-enhanced Raman scattering-based lateral flow immunoassay method for the high sensitivity simultaneous detection of anti-SARS-CoV-2 IgM/IgG. The method utilized custom SERS tags and dual layers of Raman dye, with signal intensities easily recorded by a portable Raman instrument for high sensitivity analysis. Validation on samples from COVID-19 patients and healthy individuals showed high accuracy and specificity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Microbiology
Georg Semmler, Marianna Theresia Traugott, Marianne Graninger, Wolfgang Hoepler, Tamara Seitz, Hasan Kelani, Mario Karolyi, Erich Pawelka, Sara Aragon de La Cruz, Elisabeth Puchhammer-Stockl, Stephan Walter Aberle, Karin Stiasny, Alexander Zoufaly, Judith Helene Aberle, Lukas Weseslindtner
Summary: This study found that differences in sensitivity among commercial immunoassays during acute SARS-CoV-2 infection are only partly related to the target antigen. NCP-specific IgA and IgG antibodies are detected earlier, while higher levels of S1-specific IgA antibodies occur in severely ill patients.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Virology
Arantxa Valdivia, Ignacio Torres, Dixie Huntley, Maria J. Alcaraz, Eliseo Albert, Javier Colomina, Josep Ferrer, Arturo Carratala, David Navarro
Summary: The study found that serum samples losing IgG reactivity after urea treatment were drawn significantly earlier than those which preserved it, which may help estimate the time of acquisition of infection in COVID-19 patients.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Qiaoge Bayin, Lei Huang, Chunhui Ren, Yusheng Fu, Xing Ma, Jinhong Guo
Summary: The study introduces a novel lateral flow immunoassay method based on superparamagnetic nanoparticles and giant magnetoresistance sensing system for simultaneous quantitative detection of anti-SARS-CoV-2 immunoglobulin M and G. This method offers advantages in cost-effectiveness, time efficiency, and ease of operation.
Article
Chemistry, Analytical
Jianxi Qu, Mathieu Chenier, Yushan Zhang, Chang-qing Xu
Summary: The developed MCIA technique is suitable for portable quantitative detection of SARS-CoV-2 IgM and IgG antibodies, with the characteristics of rapid, sensitive, and simple operation.
Article
Chemistry, Analytical
Zihao Xie, Shasha Feng, Fubin Pei, Mingzhu Xia, Qingli Hao, Bing Liu, Zhaoyang Tong, Jiang Wang, Wu Lei, Xihui Mu
Summary: A novel magnetic/fluorescent dual-modal lateral flow immunoassay has been developed for the rapid and accurate diagnosis of SARS-CoV-2 nucleocapsid protein. This method combines the advantages of magnetic and fluorescent signals, resulting in high sensitivity and accuracy, and shows potential for early-stage diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Infectious Diseases
Julien Exinger, Cedric Hartard, Fanny Lafferriere, Christelle Fenninger, Loic J. Charbonniere, Helene Jeulin
Summary: In this study, the lateral flow immunoassay (LFIA) COVIDTECH (R) SARS-CoV-2 IgM/IgG antibody rapid test was independently evaluated for its application in diagnosing COVID-19 caused by the new coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). The results showed that this LFIA method is suitable for serological diagnosis of SARS-CoV-2 in patients after > 14 days since the onset of symptoms.
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Analytical
Yaofeng Zhou, Yuan Chen, Wenjuan Liu, Hao Fang, Xiangmin Li, Li Hou, Yuanjie Liu, Weihua Lai, Xiaolin Huang, Yonghua Xiong
Summary: The study successfully developed highly luminescent quantum dot nanobeads for rapid and accurate measurement of total antibody concentrations in the population, which is crucial for combating the COVID-19 pandemic.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Ae Sol Lee, Su Min Kim, Kyeong Rok Kim, Chulmin Park, Dong-Gun Lee, Hyung Joon Cha, Hye Ryoung Heo, Chang Sup Kim
Summary: To control SARS-CoV-2, early diagnosis of infected individuals is crucial. A colorimetric lateral flow immunoassay (LFIA) was developed in this study, enabling sensitive detection of cultured SARS-CoV-2 in 15 minutes with a detection limit of 5 x 10^4 copies/mL. Clinical evaluation showed that the LFIA accurately detected all positive and negative samples, achieving 100% accuracy.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Microbiology
Anna Solastie, Camilla Virta, Anu Haveri, Nina Ekstrom, Anu Kantele, Simo Miettinen, Johanna Lempainen, Pinja Jalkanen, Laura Kakkola, Timothee Dub, Ilkka Julkunen, Merit Melin
Summary: This study presents an in-house FMIA serological assay for accurate detection of antibodies against SARS-CoV-2, which displayed higher specificity and sensitivity compared to other methods. Results indicate good correlation of antibody concentrations and comparability of different assays, highlighting the importance of reliable serological assays for estimating immunity persistence.
MICROBIOLOGY SPECTRUM
(2021)
Article
Infectious Diseases
Jinfeng Li, Bochao Liu, Xi Tang, Ze Wu, Jinhui Lu, Chaolan Liang, Shuiping Hou, Ling Zhang, Tingting Li, Wei Zhao, Yongshui Fu, Yuebin Ke, Chengyao Li
Summary: A quantum dot lateral flow immunoassay strip (QD-LFIA) for smartphones was designed to detect specific IgG or neutralizing antibodies in SARS-CoV-2. The results showed high sensitivity and specificity in detecting antibodies in recovered patients and vaccinated individuals, with no cross-reaction observed.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Multidisciplinary Sciences
Erica Diani, Pier Paolo Piccaluga, Virginia Lotti, Andrea Di Clemente, Marco Ligozzi, Pasquale De Nardo, Lorenza Lambertenghi, Francesca Pizzolo, Simonetta Friso, Giuliana Lo Cascio, Alice Vianello, Giacomo Marchi, Ercole Concia, Davide Gibellini
Summary: The study analyzed the diagnostic accuracy of the COVID-19 IgG/IgM rapid test cassette lateral flow immunoassay test and found that it displayed reliable performance in detecting SARS-CoV-2 infection, with a higher positivity rate observed after 10 days from symptom onset. Additionally, the LFIA assay showed valid performance compared to other LFIA assays in detecting IgM and IgG antibodies.
Article
Biochemistry & Molecular Biology
Kai-Xuan Wang, Chen Yan, Xu Yang, Pei-Yan Zhu, Wen-Wen Cui, Cong Ye, Kan Hu, Ting Lan, Lin-Yan Huang, Wan Wang, Ping Ma, Su-Hua Qi, Bing Gu, Lan Luo
Summary: In this study, we found that autophagy-mediated Sirt1 downregulation may be a promising therapeutic target for prostate cancer.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Immunology
Xiaoxiao Wu, Bing Gu, Huan Yang
Summary: The intestinal mucosa is a crucial structure for defending against pathogen infection, comprising four barriers that serve different but interconnected functions. Pathogenic bacteria have the ability to damage these barriers; however, γδT cells play a significant role in regulating and monitoring this process. Commensal bacteria are involved in the activation and migration of γδT cells to resist pathogen invasion. It has been found that the migration pattern of γδT cells is altered during pathogen invasion, leading to accelerated pathogen clearance and limited opportunistic invasion by commensal bacteria, thus maintaining intestinal health.
INTERNATIONAL REVIEWS OF IMMUNOLOGY
(2023)
Article
Chemistry, Physical
Wenqi Wang, Xingsheng Yang, Zhen Rong, Zhijie Tu, Xiaochang Zhang, Bing Gu, Chongwen Wang, Shengqi Wang
Summary: In the face of the COVID-19 pandemic, there is still a high demand for a sensitive and accurate method to detect various respiratory viruses. Researchers have proposed a multiplex lateral flow immunoassay (LFA) method using a multilayered fluorescent tag to enhance performance and amplify signals. This method allows for rapid and sensitive screening of SARS-CoV-2, influenza A virus, and human adenovirus with low detection limits, short assay time, good reproducibility, and high accuracy.
Article
Nanoscience & Nanotechnology
Zhenzhen Liu, Chongwen Wang, Shuai Zheng, Xingsheng Yang, Han Han, Yuwei Dai, Rui Xiao
Summary: In this study, a portable immunoassay based on SERS technology was proposed for simultaneous detection of multiple respiratory viruses. The method utilizes magnetic SERS tags for direct enrichment and capture of target viruses, eliminating the need for sample pretreatment and improving detection sensitivity. Experimental verification on throat swab samples demonstrated the potential of the method for early and rapid differential diagnosis of respiratory viruses.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Chemistry, Analytical
Zhijie Tu, Xingsheng Yang, Hao Dong, Qing Yu, Shuai Zheng, Xiaodan Cheng, Chongwen Wang, Zhen Rong, Shengqi Wang
Summary: In this study, a highly sensitive and multiplex lateral flow assay (LFA) was developed for the rapid screening of pathogenic bacteria. The proposed LFA method, based on Mag@QDs-WGA, enables the simultaneous detection of Pseudomonas aeruginosa and Salmonella typhimurium in complex samples. The LFA exhibited excellent analytical performance and holds great potential for on-site and real-time diagnosis of bacterial samples.
Article
Chemistry, Analytical
Ting Wu, Jiaxuan Li, Shuai Zheng, Qing Yu, Kezong Qi, Ying Shao, Chongwen Wang, Jian Tu, Rui Xiao
Summary: In this study, an ultrasensitive immunochromatographic assay (ICA) using magnetic Fe3O4@Au nanotags as a capture/detection difunctional tool was developed for the direct detection of beta 2-adrenoceptor agonists in real samples. The proposed dual-signal ICA can achieve the simultaneous determination of two illegal additives, namely, clenbuterol hydrochloride and ractopamine, with high accuracy and stability in real food samples.
Article
Engineering, Environmental
Jian Tu, Ting Wu, Qing Yu, Jiaxuan Li, Shuai Zheng, Kezong Qi, Guohui Sun, Rui Xiao, Chongwen Wang
Summary: In this study, a surface-enhanced Raman scattering (SERS)-based multiplexed lateral flow immunoassay (LFA) was developed for the simultaneous and sensitive detection of multiple drugs in complex samples. The method utilized efficient hotspots and magnetic enrichment for dual signal amplification, allowing for rapid, convenient, and sensitive drug monitoring.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Xingsheng Yang, Qing Yu, Xiaodan Cheng, Hongjuan Wei, Xiaochang Zhang, Zhen Rong, Chongwen Wang, Shengqi Wang
Summary: A flexible and ultrasensitive immunochromatographic assay (ICA) based on a colorimetric/fluorescent dual-signal enhancement strategy was proposed for the timely and accurate diagnosis of SARS-CoV-2. The method utilized stable dual-signal nanocomposites (SADQD) conjugated with spike and nucleocapsid antibodies to achieve simultaneous detection with improved sensitivity and accuracy. The biosensor provides a more accurate and convenient way to diagnose COVID-19.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Environmental Sciences
Wenqi Wang, Qing Yu, Shuai Zheng, Jiaxuan Li, Ting Wu, Shu Wang, Chongwen Wang, Bing Gu
Summary: In this study, a multiplexed and ultrasensitive immunochromatographic strip (ICS) was developed for the universal analysis of different pollutants. The method combines graphene oxide (GO) nanosheets with quantum dot (QD) loading to enhance fluorescent tagging and achieve quantitative target detection. The proposed assay demonstrates low detection limits, fast testing time, good stability, and high reproducibility, making it suitable for on-site and practical detection of small-molecule pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Wanzhu Shen, Jiaxuan Li, Bo Jiang, You Nie, Yuanfeng Pang, Chongwen Wang, Rui Xiao, Rongzhang Hao
Summary: A simple and effective immunochromatographic assay (ICA) based on silica-gold core-satellite (SiO2@20Au) surface-enhanced Raman scattering (SERS) tags was developed for sensitive and quantitative detection of Streptococcus pneumoniae (S. pneumoniae). The proposed SERS-ICA method showed a detection limit of 46 cells/mL, which was 100-fold more sensitive than the traditional AuNPs-based colorimetric ICA method. With its good stability, specificity, reproducibility, and easy operation, the SiO2@20Au-SERS-ICA has great potential for on-site and accurate detection of respiratory pathogens.
Article
Chemistry, Analytical
Qian Wang, Shuai Zheng, Yong Liu, Chongwen Wang, Bing Gu, Long Zhang, Shu Wang
Summary: In this study, a nucleic acid fluorescent lateral flow assay (NFLFA) was developed to rapidly and quantitatively detect Acinetobacter baumannii (A. baumannii) and carbapenem-resistant A. baumannii (CRAB). The combination of loop-mediated isothermal amplification (LAMP) and silica-based multilayered quantum dot nanobead tag (Si@MQB) significantly improved the sensitivity and stability of the assay. The LAMP-NFLFA achieved high detection limits and showed good repeatability and specificity in complex samples and environments, providing a promising and low-cost tool for on-site monitoring of nosocomial infections.
Article
Chemistry, Physical
Wenqi Wang, Xingsheng Yang, Zhen Rong, Zhijie Tu, Xiaochang Zhang, Bing Gu, Chongwen Wang, Shengqi Wang
Summary: This study proposes a multiplex lateral flow immunoassay method using a multilayered film-like fluorescent tag for sensitive and rapid screening of multiple respiratory viruses. The method demonstrates low detection limits, short assay time, good reproducibility, and high accuracy. The robustness of the method is validated in real saliva samples.
