Article
Biochemical Research Methods
Taylor M. Domenick, Austin L. Jones, Robin H. J. Kemperman, Richard A. Yost
Summary: To address the poor retention of highly polar metabolites in reversed-phase liquid chromatography (RPLC), a derivatization agent and an internal standard were introduced to improve analyte retention and detection limits. Multiplexed drift-tube ion mobility (IM) was integrated into the LC-MS workflow to enhance method sensitivity and selectivity. The results showed that this method allowed for the quantitation of amino acids in microglial cell extracts.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Luisa-Gabriela Bogos, Ioana-Ecaterina Pralea, Radu-Cristian Moldovan, Cristina-Adela Iuga
Summary: Chiral metabolomics is a fast-growing research field, driven by recent advances in separation techniques. This review summarizes the most significant developments in indirect enantioseparations of endogenous metabolites over the past 10 years, including improvements in chiral derivatizing agents and new separation methodologies. It also highlights exciting advancements in separation techniques combined with mass spectrometry, which offer new possibilities for characterizing chiral metabolites in complex samples and improve understanding of their roles in systems biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Christian Ieritano, J. C. Yves Le Blanc, Bradley B. Schneider, Justine R. Bissonnette, Alexander Haack, W. Scott Hopkins
Summary: A workflow was developed to analyze Verapamil and its metabolites, and it was observed that the ionogram of protonated Verapamil had two peaks while the metabolites had only one peak. This was attributed to the protonation at the tertiary amino moiety of Verapamil, which generated a chiral quaternary amine.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Shutong Yang, Liancheng Gu, Fangling Wu, Xinhua Dai, Fuxing Xu, Qiaoyu Li, Xiang Fang, Shaoning Yu, Chuan-Fan Ding
Summary: This study developed an effective strategy for enantiomeric determination of amino acids, which can easily distinguish all 19 natural amino acid enantiomers. The role of Mg2+ in host/guest interactions was also demonstrated.
Article
Biochemical Research Methods
Yang Li, Yujiao Qin, Songchang Wei, Ling Ling, Chuan-Fan Ding
Summary: Steroids are important indicators of health and disease. Differentiating between steroids, especially their isomers, can be challenging due to their structural similarities. This study presents a trapped ion mobility spectrometry-mass spectrometry (TIMS-MS) approach based on steroid analogue adduction for specific identification of isomers. The use of dexamethasone (DEX) as a heterodimer enhancer allowed successful separation of isomer pairs in TIMS.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ondrej Jurcek, Nonappa, Elina Kalenius, Pia Jurcek, Juha M. Linnanto, Rakesh Puttreddy, Hennie Valkenier, Nikolay Houbenov, Michal Babiak, Miroslav Peterek, Anthony P. Davis, Radek Marek, Kari Rissanen
Summary: Constructing structurally complex architectures using unique ligands is a challenge in metallosupramolecular chemistry, but a superchiral Pd3L6 complex can be obtained via supramolecular transmetalation reactions. These complexes can self-organize into hexagonal microparticles in aqueous media, potentially leading to the development of novel metal-organic functional materials made of natural, abundant, and relatively inexpensive steroidal compounds.
CELL REPORTS PHYSICAL SCIENCE
(2021)
Article
Biochemical Research Methods
Diana C. Velosa, Andrew J. Dunham, Marcus E. Rivera, Shon P. Neal, Christopher D. Chouinard
Summary: This study utilizes ion mobility technology to improve the analysis resolution and structural elucidation of steroids through structurally selective reactions. The results demonstrate that derivatization of hydroxyl stereoisomer pairs can significantly increase resolution, and different products can be obtained through derivatization of the carbonyl group. These changes can be easily interpreted using parameters such as accurate mass and collision cross section.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Chemistry, Analytical
Aurelie Delvaux, Estelle Rathahao-Paris, Blanche Guillon, Sophie Cholet, Karine Adel-Patient, Francois Fenaille, Christophe Junot, Sandra Alves
Summary: The study introduces a high throughput and high resolution method for characterizing main oligosaccharides in breastmilk using ion mobility spectrometry, offering high identification level. Standard HMO isomers were investigated and could be distinguished effectively. Multiple mobility peaks were observed for some HMO species, but reduction at the reducing-end did not significantly improve isomer distinction.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Xin Wang, Xiaoqi Sun, Yueying Jin, Shengyu Cheng, Yu Han, Minghui Zhang, Lingli Zhang, Xi-Ling Li, Chun-Yan Xu, Jun Zhe Min
Summary: This study introduces a novel fluorescent chiral derivatization reagent, DBD-S-M-Pro, for the sensitive determination of free chiral amino acids. The reagent targets chiral amino-functional compounds without the need for a condensation agent. A new approach was also developed for the simultaneous analysis of four chiral amino acids in human hair. The developed method showed good precision and accuracy, making it a valuable tool for analyzing free chiral amino acids in living organisms.
ANALYTICAL METHODS
(2023)
Article
Biochemical Research Methods
Joon-Yong Lee, Aivett Bilbao, Christopher R. Conant, Kent J. Bloodsworth, Daniel J. Orton, Mowei Zhou, Jesse W. Wilson, Xueyun Zheng, Ian K. Webb, Ailin Li, Kim K. Hixson, John C. Fjeldsted, Yehia M. Ibrahim, Samuel H. Payne, Christer Jansson, Richard D. Smith, Thomas O. Metz
Summary: AutoCCS is an open-source software for rapid determination of CCS values from IMS-MS measurements, demonstrating accuracy and reproducibility for standard and unknown analyte ions under various conditions.
Article
Chemistry, Applied
Yuling Li, Li Zhang, Wenjian Sun, Kuofeng Tseng, Jing Zhang, Yinlong Guo
Summary: Chiral analysis of food components can provide important information for food quality, bioactivity and safety. A high-throughput, high-sensitive and high-resolution method was developed for simultaneously determining enantiomeric ratios of multiple chiral amino acids from fermented milks. Results revealed extensive variation in chiral amino acid profiles among fermented milks and suggested the influence of different starter culture LAB on glycolysis, casein hydrolysis and enantioisomerization of amino acids.
Article
Food Science & Technology
Zewei Zhang, Hongwu Ji, Di Zhang, Shucheng Liu, Xiaoshan Zheng
Summary: In this study, the role of amino acids in the formation of volatile substances during the drying process of Penaeus vannamei was investigated. The results showed that the type and content of volatile components significantly increased in samples with moisture contents between 30% and 5%, and basic amino acids (Arg, Lys, and His) had a strong correlation with the formation of pyrazines.
Article
Chemistry, Analytical
Kevin Jeanne Dit Fouque, Alyssa Garabedian, Fenfei Leng, Yuk-Ching Tse-Dinh, Mark E. Ridgeway, Melvin A. Park, Francisco Fernandez-Lima
Summary: The study introduces a new generation trapped ion mobility spectrometer (TIMS) that is capable of trapping high-molecular-weight macromolecular assemblies with high mobility resolution and measurement of ion-neutral collision cross-section.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Anthony Abou-Dib, Frederic Aubriet, Jasmine Hertzog, Lionel Vernex-Loset, Sebastien Schramm, Vincent Carre
Summary: Petroleomics is a method that uses mass spectrometry to study bio-oils and related products, aiming to analyze the contributions of various compounds in complex samples. The latest developments have altered the nature of non-targeted analyses, but one chemical formula may hide a range of isomeric compounds, making the identification of chemical functions crucial for bio-oil upgrading.
Article
Chemistry, Analytical
Travis M. Falconer, Birgit Schneider, Carsten Baessmann, Karin Wendt, Artem Filipenko
Summary: The use of trapped ion mobility spectrometry (TIMS) in conjunction with liquid chromatography (LC) and electrospray ionization tandem mass spectrometry (MS/MS) was explored for the analysis of PDE-5 inhibitor analogs. Six out of eight compounds were resolved by LC and TIMS, while the remaining two isomers were distinguished by unique product ions in the MS/MS spectrum. The LC separation corresponded to differences in substitution on the piperazine moiety of the PDE-5 inhibitors, while the TIMS separation corresponded to the position of a nitrogen atom in the fused ring region of the molecules.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Catharina Erbacher, Nils Flothkoetter, Marcel Macke, C. Derrick Quarles, Michael Sperling, Jens Mueller, Uwe Karst
Summary: A fast and automated method for separation and quantification of bromide and 5-BrdU via IC and ICP-MS was developed, relevant for monitoring electron transfer efficiency along DNA. With a LOD of 30 ng/L, the method is suitable for screening applications with a multitude of samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Engineering, Environmental
Marcel Macke, C. Derrick Quarles, Michael Sperling, Uwe Karst
Summary: Gadolinium-based contrast agents are commonly used in MRI for improved image contrast, leading to significant environmental release of anthropogenic Gd. This study developed a rapid and efficient monitoring method utilizing a single platform system and ICP-MS for total metal analysis, successfully detecting and quantifying multiple GBCAs in surface water samples.
Article
Biochemistry & Molecular Biology
C. Derrick Quarles, Marcel Macke, Bernhard Michalke, Hans Zischka, Uwe Karst, Patrick Sullivan, M. Paul Field
Article
Chemistry, Analytical
R. Alvarez-Fernandez Garcia, M. Corte-Rodriguez, M. Macke, K. L. LeBlanc, Z. Mester, M. Montes-Bayon, J. Bettmer