Article
Chemistry, Applied
Fernanda Rodrigues Spinelli, Ana Paula Drehmer, Leonardo Valentin, Simone Nascimento, Mariana Baptistao, Regina Vanderlinde
Summary: A sample preparation approach for analyzing alpha-casein, beta-casein, albumin, and lysozyme was developed and validated in this study. The method showed high recovery values and could quantify proteins even at lower levels than the current legal limits.
Article
Chemistry, Analytical
Harvey E. Johnston, Kranthikumar Yadav, Joanna M. Kirkpatrick, George S. Biggs, David Oxley, Holger B. Kramer, Rahul S. Samant
Summary: Complete and reproducible extraction of protein is crucial for proteome analysis. Solvent precipitation SP3 (SP4) is proposed as an alternative method, which captures protein aggregates by centrifugation instead of magnetism. SP4 provides equivalent or higher protein yields and improved reproducibility. It also recovers more low-solubility and transmembrane proteins, offering higher recovery rates.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Joao Raul Belinato, Vinicius Ricardo Acquaro, Luciana Teresa Dias Cappelini, Fabio Augusto
Summary: The interest in bioactive compounds from microorganisms has increased since the first discovery, with microorganisms being a key source of biomolecules with diverse applications. While conventional sample preparation techniques are still widely used, microextraction techniques show promise in overcoming limitations and promoting a deeper understanding of complex systems and the discovery of new molecules.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Fujia Wang, Tim Veth, Marije Kuipers, Maarten Altelaar, Kelly E. Stecker
Summary: A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. The performance of the S-Trap protocol for phosphoproteomics studies is unclear, and the addition of phosphoric acid (PA) in the existing protocol is detrimental to downstream phosphopeptide enrichment. This study systematically evaluates the performance of the S-Trap digestion for proteomics and phosphoproteomics, and demonstrates that an optimized S-Trap approach, with trifluoroacetic acid substituted for PA, is a simple and effective method for phosphoproteomics sample preparation.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Fenglin Shen, Yueting Xiong, Lei Zhang, Hengchao Li, Huanhuan Zhao, Xiaohui Liu, Pengyuan Yang
Summary: A new rapid and simple sample preparation method was developed for serum proteomic studies, allowing for efficient identification of multiple proteins in under 2 hours. This method has the potential to accelerate biomarker discovery and clinical application in serum protein research.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
John B. Coulton, James L. Edwards
Summary: The sample preparation technique described in the study involves online filter-assisted electroporation-based cell lysis and chilled organic solvent extraction for rapid preparation of metabolomic samples from suspended cells. By using isotopically labeled internal standards and C-13-glucose labeling experiments, the method ensures enzymatic quenching and successful incorporation of C-13 into organic acid metabolites. Analysis by nano-reverse-phase liquid chromatography-mass spectrometry (nano-RP-LC-MS) demonstrates the effectiveness of the technique in studying metabolites such as itaconate.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Hae-Ni Jung, Da-Hee Park, Kyung-Hee Yoo, Hee-Jung Cho, Jae-Han Shim, Ho-Chul Shin, A. M. Abd El-Aty
Summary: An analytical method for the simultaneous determination of 12 anthelmintics in fishery products was developed and proved suitable for real sample testing. This method utilized liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry and exhibited moderate matrix effects.
Article
Chemistry, Analytical
Slavka Nagyova, Peter Tolgyessy, Milan Laurencik, Michal Kirchner
Summary: The study introduced a new sample preparation method combined with gas chromatography-triple quadrupole tandem mass spectrometry for determining polycyclic aromatic hydrocarbons in dried gammarid samples. The method showed high sensitivity, precision, and recovery rates, making it suitable for monitoring and controlling compliance with environmental quality standards for specific PAHs in surface water biota.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemical Research Methods
Dong-Gi Mun, Neha S. Joshi, Rohit Budhraja, Gunveen S. Sachdeva, Taewook Kang, Firdous A. Bhat, Husheng Ding, Benjamin J. Madden, Jun Zhong, Akhilesh Pandey
Summary: This study compared the performance of an automated platform, AccelerOme, with manual sample processing. The results demonstrated that the automated platform performs at a level similar to a manual process performed by an expert for TMT-based proteomics. It has the potential to replace manual pipelines and be applied to large-scale TMT-based studies, providing robust results and high sample throughput.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Spectroscopy
Shan Xing, Maoyi Luo, Ni Yuan, Daqian Liu, Yao Yang, Xiongxin Dai, Weichao Zhang, Ning Chen
Summary: This study validated the effectiveness of three commonly used sample digestion techniques in analyzing Pu isotopes from global atmospheric fallout, and found that purification using specific resins can reduce the influence of major interference elements, thereby improving the accurate measurement of Pu.
ATOMIC SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Tanja Bien, Sebastian Bessler, Klaus Dreisewerd, Jens Soltwisch
Summary: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has the potential to visualize the spatial distribution of biomolecules in cell cultures and tissue sections with high resolution, but sample preparation is crucial. A study found that a robust sample preparation protocol can preserve cell morphology and molecular integrity, contributing to the study of cell-to-cell heterogeneities at a molecular level. Advancements in single-cell MALDI imaging could benefit from these protocols.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Lianlian Ma, Qingrong Xie, Mingyi Du, Yudi Huang, Yingying Chen, Dong Chen, Yizhu Xu, Hanhong Xu, Xinzhou Wu, Zhibin Yin
Summary: This study systematically investigated the optimized sample preparation conditions for insects and model organisms in mass spectrometry imaging (MSI), providing guidelines for MSI analysis and expanding its potential in life sciences and environmental sciences.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Chin-Wen Chen, Chia-Feng Tsai, Miao-Hsia Lin, Shu-Yu Lin, Chuan-Chih Hsu
Summary: Plant phosphoproteomics provides a global view of phosphorylation-mediated signaling in plants, but high-throughput methods with sensitive detection and accurate quantification are needed. In this study, we developed a tandem S-Trap-IMAC method based on suspension trapping, which improved the coverage and quantification accuracy of the Arabidopsis phosphoproteome compared to precipitation-based workflow. We applied this method to study ABA signaling in Arabidopsis seedlings and quantified key phosphorylation sites on core ABA signaling components across different time points, revealing the importance of multiply phosphorylated peptides in early ABA signaling. Our optimized workflow enables high-throughput phosphoproteome profiling of low-input plant samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Vidur Kailash, Luis Mendoza, Robert L. Moritz, Michael R. Hoopmann
Summary: SPACEPro is a tool developed to evaluate the efficiency and reproducibility of protein cleavage in peptide samples, providing comprehensive information on sample processing to peptides at the PSM, peptide, and protein levels. It helps users understand the quality of sample processing efficiency and develop protocols for improvement with minimal effort.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Food Science & Technology
Vanessa G. da Silveira, Mauricio S. Oliveira, Carlos A. A. de Almeida, Rodrigo B. Hoff, Carlos A. Mallmann
FOOD ANALYTICAL METHODS
(2018)
Article
Environmental Sciences
Juliana Bazzan Arsand, Rodrigo Barcellos Hoff, Louise Jank, Lucas N. Meirelles, M. Silvia Diaz-Cruz, Tania Mara Pizzolato, Damia Barcelo
SCIENCE OF THE TOTAL ENVIRONMENT
(2018)
Article
Chemistry, Applied
Louise Jank, Magda Targa Martins, Juliana Bazzan Arsand, Marco Flores Ferrao, Rodrigo Barcellos Hoff, Fabiano Barreto, Tania Mara Pizzolato
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2018)
Article
Food Science & Technology
Marcia H. S. Kurz, Juliana L. da S. Batista, Lenise G. de Oliveira, Rodrigo Hoff, Manoel L. Martins, Fabio F. Goncalves
FOOD ANALYTICAL METHODS
(2019)
Article
Agriculture, Multidisciplinary
Luciano Molognoni, Jacson Nascimento dos Santos, Cristian Rafael Kleemann, Ana Carolina Oliveira Costa, Rodrigo Barcellos Hoff, Heitor Daguer
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Environmental Sciences
Rodrigo Hoff, Ricardo Furtado, Juliane Marques dos Santos, Eduardo Saldanha Vogelmann, Gabriel Duarte Hoff, Rafaella Cunha Kneip, Louise Jank, Fabio Ferreira Goncalves
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Biochemical Research Methods
Luciano Molognoni, Heitor Daguer, Rodrigo Barcellos Hoff, Janh Rodrigues, Antonio Carlos Joussef, Juliano De Dea Lindner
JOURNAL OF CHROMATOGRAPHY A
(2020)
Article
Environmental Sciences
Rodrigo Hoff, Antonio Dias Echeverria, Gabriel Duarte Hoff, Rafaella Cunha Kneip, Louise Jank, Juliana Arsand, Fabio Ferreira Goncalves
Article
Environmental Sciences
Juliana Bazzan Arsand, Rodrigo Barcellos Hoff, Louise Jank, Roberta Bussamara, Alexsandro Dallegrave, Fatima Menezes Bento, Livia Kmetzsch, Daiane Acosta Falcao, Maria do Carmo Ruaro Peralba, Adriano de Araujo Gomes, Tania Mara Pizzolato
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Food Science & Technology
Thais Cardoso Merlo, Luciano Molognoni, Rodrigo Barcellos Hoff, Heitor Daguer, Iliani Patinho, Carmen J. Contreras-Castillo
Summary: A novel method was developed for the determination of PAHs in smoked bacon using pressurized liquid extraction and LC-MS/MS, with satisfactory performance evaluated based on various parameters. The method proved to be fast, cheap, efficient, and reliable when applied to real samples, with PAHs levels below the maximum limits suggested by the Commission Regulation.
Article
Biochemical Research Methods
Rodrigo Barcellos Hoff, Luciano Molognoni, Carolina Turnes Pasini Deolindo, Maryella Osorio Vargas, Cristian Rafael Kleemann, Heitor Daguer
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2020)
Article
Environmental Sciences
Rodrigo Barcello Hoff, Luciano Molognoni, Carolina Turnes Pasini Deolindo, Thais de Oliveira, Joana Leticia Sarda Mattos, Luan Valdemiro Alves de Oliveira, Heitor Daguer
Summary: Sugarcane yeast and brewer's yeast are commonly used in animal feed in Brazil, but the risk of antibiotic residues in these yeasts could lead to non-compliant feed products and potential toxicity to animals.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2021)
Article
Food Science & Technology
Gabriela Soster Santetti, Marina Volpato Dacoreggio, Heloisa Patricio Inacio, Barbara Biduski, Rodrigo Barcellos Hoff, Carlise Beddin Fritzen Freire, Luiz Carlos Gutkoski, Renata Dias de Mello Castanho Amboni
Summary: This study investigated the effect of fermentation times on dough made with whole wheat flour added with yerba mate on the release of phenolic compounds and antioxidant activity. The results showed that fermentation time played a crucial role in releasing phenolic compounds in the dough.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Luciano Molognoni, Leandro Antunes de Sa Ploencio, Carolina Turnes Pasini Deolindo, Luan Valdemiro Alves de Oliveira, Rodrigo Barcellos Hoff, Heitor Daguer
MICROCHEMICAL JOURNAL
(2020)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
