Article
Biology
Alexander L. Tesmer, Nicholas P. Fields, Estuardo Robles
Summary: This study reveals a novel role for TL feedback projections in driving binocular integration and spatial summation in tectal PyrNs in zebrafish. The research demonstrates the encoding of gradual changes in scene luminance in PyrNs and the determination of PyrN functional classes by RGC input and TL input.
Article
Cell Biology
Yuki Shimizu, Takashi Kawasaki
Summary: The study compared the neural stem cell responses and regenerative capacity in the optic tectum of adult medaka and zebrafish after injury, finding limited neuronal generation and scar formation in injured medaka. This suggests that adult medaka brain has low regenerative capacity and represents an attractive model for investigating critical factors for brain regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Emma Alexander, Lanya T. Cai, Sabrina Fuchs, Tim C. Hladnik, Yue Zhang, Venkatesh Subramanian, Nicholas C. Guilbeault, Chinnian Vijayakumar, Muthukumarasamy Arunachalam, Scott A. Juntti, Tod R. Thiele, Aristides B. Arrenberg, Emily A. Cooper
Summary: This study demonstrates that different species exhibit spatial biases when estimating self-motion, possibly as a strategy to adapt to noisy natural environments. Observations on larval zebrafish suggest that the lateral regions of the lower visual field provide the most informative signals for estimating swimming speed.
Article
Cell Biology
Yuki Shimizu, Mariko Kiyooka, Toshio Ohshima
Summary: Adult zebrafish have a high capacity for CNS regeneration compared to mammals, with stab wound injury inducing radial glia proliferation and differentiation into new neurons. Histological analysis showed an increase in macrophages prior to RG proliferation, with IL6/Stat3 signaling identified as the initial trigger of RG activation during optic tectum regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Sierra C. Dixon, Bailey J. Calder, Shane M. Lilya, Brandon M. Davies, Annalie Martin, Maggie Peterson, Jason M. Hansen, Arminda Suli
Summary: The mammalian superior colliculus and its non-mammalian homolog, the optic tectum (OT), are midbrain structures that integrate multimodal sensory inputs and guide non-voluntary movements in response to prevalent stimuli. Recent studies have implicated this structure as a possible site affected in autism spectrum disorder (ASD). Interestingly, fetal exposure to valproic acid (VPA) has also been associated with an increased risk of ASD in humans and animal models. Therefore, we took the approach of determining the effects of VPA treatment on zebrafish OT development as a first step in identifying the mechanisms that allow its formation. We describe normal OT development during the first 5 days of development and show that in VPA-treated embryos, neuronal specification and neuropil formation was delayed. VPA treatment was most detrimental during the first 3 days of development and did not appear to be linked to oxidative stress. In conclusion, our work provides a foundation for research into mechanisms driving OT development, as well as the relationship between the OT, VPA, and ASD.
Article
Neurosciences
Annalie Martin, Anne Babbitt, Allison G. Pickens, Brett E. Pickett, Jonathon T. Hill, Arminda Suli
Summary: In this study, single-cell RNA sequencing (scRNA-seq) was used to characterize the transcriptomic profiles of tectal cells in larval zebrafish. The results revealed the presence of at least 25 transcriptionally distinct cell populations in the optic tectum (OT), including mature and developing neuronal populations as well as non-neuronal cell types. These findings provide valuable insights into the diversity and function of tectal cell types.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Cell Biology
Elisabeth C. DeMarco, George R. Stoner, Estuardo Robles
Summary: This study developed a genetic labeling system to study dendritic spine development in larval zebrafish, revealing the concurrent growth of PyrN dendrites and spine formation. It also found pronounced defects in dendrite growth and spine stabilization in PyrNs of fmr1 mutant zebrafish.
DISEASE MODELS & MECHANISMS
(2022)
Article
Multidisciplinary Sciences
David C. Schultz, Robert M. Johnson, Kasirajan Ayyanathan, Jesse Miller, Kanupriya Whig, Brinda Kamalia, Mark Dittmar, Stuart Weston, Holly L. Hammond, Carly Dillen, Jeremy Ardanuy, Louis Taylor, Jae Seung Lee, Minghua Li, Emily Lee, Clarissa Shoffler, Christopher Petucci, Samuel Constant, Marc Ferrer, Christoph A. Thaiss, Matthew B. Frieman, Sara Cherry
Summary: This study screened and validated 122 drugs with antiviral activity and selectivity against SARS-CoV-2 out of approximately 18,000 drugs. Among them are the approved antivirals remdesivir and molnupiravir. The study also found that combining pyrimidine biosynthesis inhibitors with antiviral nucleoside analogues synergistically inhibits SARS-CoV-2 infection.
Article
Nutrition & Dietetics
Amna Emhemed Abdurrahim, Vera C. Mazurak, Lingyun Chen
Summary: Oxidative stress is a major factor in the development of chronic health conditions, and phytochemicals have shown to have antioxidant properties. This study found that a combination of anthocyanins and gingerols can enhance the cellular antioxidant response and provide cellular protection against oxidative stress. The combination promotes the cellular antioxidant defense systems, reduces harmful substances generated by oxidative stress, and increases the levels of antioxidant compounds. This research highlights the potential importance of developing functional foods or nutraceutical products with the combination of anthocyanins and gingerols.
FRONTIERS IN NUTRITION
(2023)
Article
Multidisciplinary Sciences
Olivia L. Hagen, Yehyun Kim, Elaine Kushkowski, Hannah Rouse, Kara L. Cerveny
Summary: Zebrafish have remarkable lifelong growth and regenerative abilities, with specialized stem cell niches supporting continuous growth of the visual system. Surgical removal of one eye in zebrafish larvae allows for comparison of innervated and denervated tectal lobes, providing insights into neural regulation.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Agriculture, Dairy & Animal Science
Shuman Huang, Xiaoke Niu, Jiangtao Wang, Zhizhong Wang, Huaxing Xu, Li Shi
Summary: The visual system of domestic pigeons can quickly and accurately detect moving objects. Neurons in the optic tectum of pigeons show faster and stronger responses to moving stimuli compared to flashed ones, possibly due to a spatiotemporal integration process.
Article
Biology
Tania Moreno-Marmol, Mario Ledesma-Terron, Noemi Tabanera, Maria Jesus Martin-Bermejo, Marcos J. Cardozo, Florencia Cavodeassi, Paola Bovolenta
Summary: The development of the vertebrate eye involves the reorganization of embryonic tissues to form a round shape, with the retinal pigment epithelium (RPE) playing a key role in this process. Studies in zebrafish have shown that RPE cells stretch and flatten to match the curvature of the retina, promoting the formation of the optic cup. This mechanism differs in species with slower development, such as mice and humans, where RPE expansion occurs through cell proliferation over a longer period of time.
Review
Neurosciences
Shuyu I. Zhu, Geoffrey J. Goodhill
Summary: A key challenge for neural systems is to extract relevant information from the environment and make appropriate behavioral responses. The larval zebrafish offers an exciting opportunity for studying these processes. Prey hunting behavior requires the brain to extract and combine sensory input and form motor outputs. The transparency of the larval zebrafish brain allows for optical recording of whole-brain activity to reveal neural mechanisms involved in hunting.
FRONTIERS IN NEURAL CIRCUITS
(2023)
Article
Immunology
Qing Yang, Amanda C. Burkardt, Lakshimi T. Sunkara, Kan Xiao, Guolong Zhang
Summary: The study found that certain natural cyclooxygenase-2 (COX-2) inhibitors can enhance the expression of host defense peptides (HDP) genes in chickens. These inhibitors demonstrated a strong synergistic effect with butyrate in increasing the expressions of multiple HDP genes. Additionally, they promoted the expressions of barrier function-related genes and suppressed inflammatory responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Instruments & Instrumentation
Thomas Kissinger, Stephen W. James, Simone Weber, Kevin Mullaney, Edmond Chehura, Huseyin H. Pekmezci, James H. Barrington, Stephen E. Staines, Thomas O. H. Charrett, Nicholas J. Lawson, Mudassir Lone, Richard Atack, Ralph P. Tatam
Summary: This paper investigates the application of optical fibre strain and shape measurement sensors on a rotor blade during a full-speed helicopter ground run. Real-time data is wirelessly streamed from rotor hub-mounted sensor interrogators, and two-dimensional direct fibre-optic shape sensing (DFOSS) using fibre segment interferometry-based interrogation is explored. The concept of visualizing blade shape change over one rotation period using rotation displacement surfaces is introduced, and the usefulness of DFOSS data in determining operational modal frequencies independently for both horizontal and vertical vibration directions of the blade is demonstrated.
SMART MATERIALS AND STRUCTURES
(2022)
Article
Multidisciplinary Sciences
Matthias C. Vogg, Leonardo Beccari, Laura Iglesias Olle, Christine Rampon, Sophie Vriz, Chrystelle Perruchoud, Yvan Wenger, Brigitte Galliot
NATURE COMMUNICATIONS
(2019)
Article
Neurosciences
H. Kaddour, E. Coppola, A. A. Di Nardo, C. Le Poupon, P. Mailly, A. Wizenmann, M. Volovitch, A. Prochiantz, A. Pierani
Article
Chemistry, Multidisciplinary
Chenge Li, Alison G. Tebo, Marion Thauvin, Marie-Aude Plamont, Michel Volovitch, Xavier Morin, Sophie Vriz, Arnaud Gautier
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Biochemistry & Molecular Biology
Alison G. Tebo, Benjamien Moeyaert, Marion Thauvin, Irene Carlon-Andres, Dorothea Boken, Michel Volovitch, Sergi Padilla-Parra, Peter Dedecker, Sophie Vriz, Arnaud Gautier
Summary: Spectrally separated fluorophores enable the observation of multiple targets in living cells simultaneously for a deeper understanding of cell functions. Engineered fluorogen-activating tags based on the FAST system offer orthogonality in recognition and spectral properties, allowing efficient multicolor imaging of proteins in live cells.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Cell Biology
Marion Thauvin, Rodolphe Matias de Sousa, Marine Alves, Michel Volovitch, Sophie Vriz, Christine Rampon
Summary: Reactive oxygen species (ROS), including hydrogen peroxide (H2O2), play a crucial role in cell signaling. In zebrafish fin regeneration, the interaction between H2O2 and Sonic hedgehog (Shh) is studied, with H2O2 controlling Shh expression and Shh regulating the H2O2 level. This interaction changes during different stages of the regenerative program.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Marion Thauvin, Irene Amblard, Christine Rampon, Aurelien Mourton, Isabelle Queguiner, Chenge Li, Arnaud Gautier, Alain Joliot, Michel Volovitch, Sophie Vriz
Summary: Hydrogen peroxide (H2O2) has recently emerged as a central molecule that links environment, cell metabolism, and cell signaling. This study reveals that H2O2 levels play a crucial role in the delivery of the morphogen Sonic hedgehog (Shh), impacting its secretion, endocytosis, and distribution. Additionally, a feedback loop exists between Shh trafficking and H2O2 synthesis. These findings provide molecular insights into the robustness of morphogenesis.
Article
Endocrinology & Metabolism
Ayaka Zasu, Futa Hishima, Marion Thauvin, Yosuke Yoneyama, Yoichiro Kitani, Fumihiko Hakuno, Michel Volovitch, Shin-Ichiro Takahashi, Sophie Vriz, Christine Rampon, Hiroyasu Kamei
Summary: The study investigates the effects of hypoxia and re-oxygenation on embryonic growth using the zebrafish model. Hypoxia slows down embryonic growth, while re-oxygenation induces catch-up growth. The mitogen-activated kinase pathway and the generation of reactive oxygen species play a role in the catch-up growth induced by re-oxygenation. Furthermore, the role of Nox-dependent hydrogen peroxide production is revealed in the MAPK-activation and catch-up growth.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Christine Rampon, Sophie Vriz