Article
Chemistry, Multidisciplinary
Alexander S. Zhovmer, Alexis Manning, Chynna Smith, James B. Hayes, Dylan T. Burnette, Jian Wang, Alexander X. Cartagena-Rivera, Nikolay V. Dokholyan, Rakesh K. Singh, Erdem D. Tabdanov
Summary: The study demonstrates an antagonistic mechanical balance within the dynein-kinesin microtubular motor system, where dynein activity drives inward compaction of the microtubular network, while kinesins bundle and expand MTs into giant circular bands. This balance controls the 3D architecture, mechanics, and cell-microenvironment interactions of cells through orthogonal mechanisms of MT network reorganization.
Article
Chemistry, Multidisciplinary
Yerbol Tagay, Sina Kheirabadi, Zaman Ataie, Rakesh K. Singh, Olivia Prince, Ashley Nguyen, Alexander S. Zhovmer, Xuefei Ma, Amir Sheikhi, Denis Tsygankov, Erdem D. Tabdanov
Summary: The principal cause of death in cancer patients is metastasis, which is conventionally linked to actomyosin-driven cell locomotion. However, this study identifies a complementary mechanism of metastatic locomotion powered by dynein-generated forces. These findings provide new insights into the fundamental understanding of cell locomotion mechanisms and expand the spectrum of clinical targets against metastasis.
Article
Multidisciplinary Sciences
Eunsun Ko, Dasom Kim, Dong Wha Min, Seung-Hae Kwon, Ji-Yun Lee
Summary: Nrf2 is a important regulator of antioxidant and anti-inflammatory enzymes, interacting with Keap1 to affect the transcription of cytoprotective enzymes. The Nrf2/Keap1 pathway may impact cell motility through dysregulation of the RhoA-ROCK1 signaling pathway in non-small cell lung cancer cells.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Lisheng Fu, Xiaoyan Wang, Ying Yang, MeiHua Chen, Adilijiang Kuerban, Haojie Liu, Yiwei Dong, QianQian Cai, Mingzhe Ma, XingZhong Wu
Summary: SEPT11 plays a crucial role in hepatocellular carcinoma (HCC) by promoting metastasis and regulating cytoskeleton rearrangement. It is highly expressed in various tumors, including HCC, and associated with poor prognosis. SEPT11 acts as an oncogene in HCC and could be a potential therapeutic target.
CELL DEATH & DISEASE
(2023)
Article
Biophysics
Karishma Smart, Adam H. Kramer, Sachin Smart, Louis Hodgson, David J. Sharp
Summary: The microtubule cytoskeleton and its dynamics, regulated by FL2, play a crucial role in cell migration. Knockdown of FL2 leads to increased cell motility by decreasing activation of p21 Rho GTPase and its activator GEF-H1.
BIOPHYSICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Xiangyu Fan, Richard J. McKenney
Summary: This study reveals the crucial role of a specialized kinesin tail domain in directing motor transport along specific microtubule tracks. The tail domain affects the initial motor-microtubule interaction and the processive motility, and its effect is enhanced when the motor domain is in the ADP state.
NATURE COMMUNICATIONS
(2023)
Review
Neurosciences
Abhishek Roy, Zarna Pathak, Hemant Kumar
Summary: The difficulties in regenerating after CNS injuries, including spinal cord injury, are attributed to factors such as hypertrophic scarring, inhibitory molecules, and the absence of mechanisms responsible for axonal regeneration in the adult CNS. Strategies to improve axon regeneration include neutralizing inhibitors/proteins and stabilizing microtubules. Furthermore, the focus on counteracting inhibitors of axonal growth and their downstream signaling through the RhoA/ROCK pathway is emphasized.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemical Research Methods
Austin E. Y. T. Lefebvre, Dennis Mai, Kai Kessenbrock, Devon A. Lawson, Michelle A. Digman
Summary: Mitometer is an algorithm for fast, unbiased, and automated segmentation and tracking of mitochondria in live-cell time-lapse images, which can identify mitochondrial motion and morphology, including fusion and fission events. Using Mitometer, it was found that mitochondria in triple-negative breast cancer cells are faster, more directional, and more elongated than those in receptor-positive counterparts. Furthermore, mitochondrial motility and morphology in breast cancer, but not in normal breast epithelia, correlate with metabolic activity.
Article
Microbiology
Adam Haines, Jordan Wesolowski, Nathan M. Ryan, Tiago Monteiro-Bras, Fabienne Paumet
Summary: Chlamydia trachomatis manipulates host actin and microtubule networks to survive intracellularly, with the effector InaC playing a key role in coordinating these processes. The interaction between ARF1 and RhoA mediated by InaC regulates the stability and formation of microtubule and actin scaffolds, shedding light on new cellular signaling pathways exploited by this clinically significant pathogen.
Article
Multidisciplinary Sciences
Robert A. Law, Alexander Kiepas, Habben E. Desta, Emiliano Perez Ipina, Maria Parlani, Se Jong Lee, Christopher L. Yankaskas, Runchen Zhao, Panagiotis Mistriotis, Nianchao Wang, Zhizhan Gu, Petr Kalab, Peter Friedl, Brian A. Camley, Konstantinos Konstantopoulos
Summary: Cells regulate epithelial integrity and cancer metastasis through adherens junction dynamics. This study shows that the spatial confinement of peritumor stroma promotes metastatic cell dissemination by disrupting cell-cell adhesion. The mechanism involves myosin IIA dismantling E-cadherin junctions, which is controlled by RhoA/ROCK activation mediated by nucleocytoplasmic shuttling of RacGAP1 and Ect2 and increased microtubule dynamics.
Article
Cell Biology
Xinyan Jiao, Bo Wang, Lu Yang, Qingbin Zhao, Miao Zhang, Xiaoxu Liu, Can Zhou, Ruiqi Wang, He Chen, Jichang Wang, Yu Ren, Peijun Liu
Summary: This study found that FMNL2 suppressed cell migration and invasion of breast cancer by inhibiting the RhoA/LIMK/Cofilin pathway through a reduction of cytoplasmic p27. The expression of FMNL2 was associated with ER in breast cancer. Interfering with the FMNL2-mediated RhoA/LIMK/Cofilin pathway involving cytoplasmic p27 may be a promising strategy for improving breast cancer metastasis and prognosis.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Giulia Villari, Noemi Gioelli, Donatella Valdembri, Guido Serini
Summary: Cell adhesion to the extracellular matrix (ECM) plays a crucial role in the development, functioning, and repair of metazoans. Disruption of this adhesion can lead to various diseases. Integrins, as major ECM receptors, are involved in the transportation and positioning process of the plasma membrane. Microtubules and associated motors play a key role in driving vesicular transport and maintaining the asymmetric adhesion between cells and ECM, influencing the establishment of cell polarity.
Article
Biochemistry & Molecular Biology
Valerie Siahaan, Ruensern Tan, Tereza Humhalova, Lenka Libusova, Samuel E. Lacey, Tracy Tan, Mariah Dacy, Kassandra M. Ori-McKenney, Richard J. McKenney, Marcus Braun, Zdenek Lansky
Summary: Tau and MAP2 can form envelopes on microtubules by altering lattice spacing, providing a molecular basis for spatial regulation of microtubule-based processes. Envelopes can affect the activity of other MAPs and motor proteins on microtubules.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Emre Kusakci, Zaw Min Htet, Yuanchang Zhao, John P. Gillies, Samara L. Reck-Peterson, Ahmet Yildiz
Summary: This study reveals that the binding of Lis1 to the AAA+ ring of dynein slows down its motility without affecting force generation. Additionally, Lis1 binding to the stalk of dynein prolongs its stalls and reduces the asymmetry in force-induced detachment from microtubules.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jinjin Duan, Li Wang, Liqun Shang, Shumei Yang, Hua Wu, Yongcheng Huang, Yi Miao
Summary: The study found that TNS1 is upregulated in NSCLC cells and tissues, and is associated with the prognosis of NSCLC patients. Additionally, TNS1 promotes the growth and metastasis of NSCLC cells via the Akt/mTOR/RhoA pathway. miR-152 targets TNS1 to influence the progression of NSCLC.
BIOSCIENCE REPORTS
(2021)
Article
Cell Biology
Nicolas Molinie, Svetlana N. Rubtsova, Artem Fokin, Sai P. Visweshwaran, Nathalie Rocques, Anna Polesskaya, Anne Schnitzler, Sophie Vacher, Evgeny Denisov, Lubov A. Tashireva, Vladimir M. Perelmuter, Nadezhda Cherdyntseva, Ivan Bieche, Alexis M. Gautreau
Article
Plant Sciences
I. Fokin Artem, N. Zhapparova Olga, V. Burakov Anton, S. Nadezhdina Elena
Article
Biochemistry & Molecular Biology
M. S. Vildanova, A. A. Saidova, A. I. Fokin, D. M. Potashnikova, G. E. Onishchenko, E. A. Smirnova
BIOCHEMISTRY-MOSCOW
(2019)
Review
Cell Biology
Anton Burakov, Elena S. Nadezhdina
Article
Cell Biology
Anton Burakov, Ivan Vorobjev, Irina Semenova, Ann Cowan, John Carson, Yi Wu, Vladimir Rodionov
Summary: The study reveals that in areas with low microtubule density, microtubule plus ends persistently grow or pause, while in dense areas they frequently transition to shortening. This indicates the existence of a density-dependent mechanism regulating microtubule dynamics.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Cell Biology
Olga Shomron, Koret Hirschberg, Anton Burakov, Rimma Kamentseva, Elena Kornilova, Elena Nadezhdina, Ilya Brodsky
Summary: The endoplasmic reticulum (ER) is involved in the biogenesis, modification, and transport of secreted and membrane proteins. Transport carriers (TCs) form at ER exit sites (ERES) via a COPII-dependent mechanism and move to the Golgi apparatus on microtubule tracks. Dynein activity mediates the central concentration of ERES near the Golgi, enhancing the efficiency of ER-to-Golgi transport.
Article
Multidisciplinary Sciences
Artem Fokin, Violaine David, Ksenia Oguievetskaia, Emmanuel Derivery, Caroline E. Stone, Luyan Cao, Nathalie Rocques, Nicolas Molinie, Veronique Henriot, Magali Aumont-Nicaise, Maria-Victoria Hinckelmann, Frederic Saudou, Christophe Le Clainche, Andrew P. Carter, Guillaume Romet-Lemonne, Alexis M. Gautreau
Summary: The WASH complex interacts with dynactin through its FAM21 subunit and uncaps it, inducing the branching reaction of the Arp2/3 complex. The WASH complex plays an important role in coordinating two complexes containing actin-related proteins.
Article
Biochemistry & Molecular Biology
Gleb Simanov, Irene Dang, Artem I. Fokin, Ksenia Oguievetskaia, Valerie Campanacci, Jacqueline Cherfils, Alexis M. Gautreau
Summary: In the process of cell migration, Arpin interacts with Tankyrase 1 and 2 (TNKS) and Arp2/3 to regulate migration persistence through two independent pathways. The mutation affecting interactions with both TNKS and Arp2/3 renders Arpin completely inactive, suggesting the importance of these interactions in controlling migration persistence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Artem I. Fokin, Alexis M. Gautreau
Summary: This article discusses the generation of branched actin networks by the Arp2/3 complex in different locations of the cell, as well as the activation of the complex by WASH and WAVE. Whether at the surface of endosomes or the cell cortex, both NPFs require coordination with other multiprotein complexes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kim Adameyko, Anton Burakov, Alexander D. Finoshin, Kirill Mikhailov, Oksana Kravchuk, Olga S. Kozlova, Nicolay G. Gornostaev, Alexander Cherkasov, Pavel A. Erokhov, Maria I. Indeykina, Anna E. Bugrova, Alexey S. Kononikhin, Andrey Moiseenko, Olga S. Sokolova, Artem N. Bonchuk, Irina Zhegalova, Anton A. Georgiev, Victor S. Mikhailov, Natalia E. Gogoleva, Guzel R. Gazizova, Elena Shagimardanova, Oleg A. Gusev, Yulia Lyupina
Summary: Ferritins are a conservative family of proteins found in all species, playing essential roles in resistance to redox stress, immune response, and cell differentiation. The study characterized ferritins in two cold-water sponges, revealing their regulation by cellular iron and oxygen levels, and their importance in various biological processes. Further research is needed to explore the function of atypical ferritins lacking iron-binding capacity in invertebrate species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Artem I. Fokin, Roman N. Chuprov-Netochin, Alexander S. Malyshev, Stephane Romero, Marina N. Semenova, Leonid D. Konyushkin, Sergey V. Leonov, Victor V. Semenov, Alexis M. Gautreau
Summary: Several compounds structurally related to CK-666 were found to suppress Arp2/3 activation, and two of them demonstrated improved in vivo efficiency compared to CK-666.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ilya B. Brodsky, Artem I. Fokin, Aleksei A. Efremov, Elena S. Nadezhdina, Anton V. Burakov
Summary: Membrane trafficking in interphase animal cells is carried out along microtubules, with the Golgi and centrosome serving as important microtubule organizing centers. Recent studies have revealed the role of Golgi-derived microtubules, but the differential expression of proteins and potential participants in this process are still unknown.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Desislava L. Makeeva, Claire V. Riggs, Anton A. Burakov, Pavel S. Ivanov, Artem A. Kushchenko, Dmitri I. Bykov, Vladimir S. Popenko, Vladimir V. Prassolov, Pavel V. E. Ivanov, Sergey Dmitriev
Summary: Upon oxidative stress, mammalian cells reprogram their translation and form stress granules (SGs). Arsenite-induced stress increases stop-codon readthrough rate and translation reinitiation levels on uORF-containing and bicistronic mRNAs. Translation termination factors, ribosome recycling, and translation reinitiation factors are recruited to SGs during arsenite treatment. This spatial redistribution of translation factors may contribute to mRNA translation resumption and suggest the presence of post-termination, recycling, or reinitiation complexes in SGs.
Article
Developmental Biology
O. Kravchuk, A. Burakov, N. G. Gornostaev, K. Mikhailov, K. Adameyko, A. D. Finoshin, A. A. Georgiev, V. S. Mikhailov, Y. E. Yeryukova, G. A. Rubinovsky, D. Zayts, G. R. Gazizova, O. A. Gusev, E. Shagimardanova, Y. Lyupina
Summary: The acetylation/deacetylation of lysine is a common epigenetic modification of proteins in eukaryotes, with histone deacetylases (HDACs) playing a key role in regulating various biological processes, possibly including cell transdifferentiation.
RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
