Review
Biochemistry & Molecular Biology
Vahid Rahimkhoei, Jafar Rezaie, Ali Akbari, Aynaz Nourani, Nassrollah Jabbari, Zohreh Mehri Lighvan, Mojtaba Amini
Summary: This review article discusses the importance of rapid and accurate diagnosis of 2019-nCoV, highlighting advancements in nano-based techniques that are showing comparable results with traditional PCR and CT methods.
CELL BIOCHEMISTRY AND FUNCTION
(2021)
Letter
Medical Laboratory Technology
Weiping Liu, Xiaolong Guo, Jianping Deng, Yaohui Song, Minggang Yin, Chengli Zhang, Xia Long, Chengjian Cao
Summary: The accuracy of nucleic acid detection for SARS-CoV-2 can be impacted by various factors, including the use of unmatched virus preservation solution which may lead to incorrect clinical diagnosis.
CLINICAL LABORATORY
(2021)
Article
Virology
Asmaa M. Altamimi, Dalia A. Obeid, Taghreed A. Alaifan, Moroje T. Taha, Marwa T. Alhothali, Fahad A. Alzahrani, Ahmad M. Albarrag
Summary: The study focuses on the clinical and analytical performance of SARS-CoV-2 (RT-PCR) commercial kits, with four kits showing a sensitivity of 100% and all tested kits scoring above 95% in specificity. Most of the commercially available RT-PCR kits in the study can be used for routine diagnosis of patients with SARS-CoV-2.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medical Laboratory Technology
Koji Tsuchiya, Yoshie Hosaka, Toshihiro Takahashi, Masayoshi Chonan, Yuko Makita, Isana Katayama, Tomohiko Ai, Mayumi Idei, Yuki Horiuchi, Kotoko Yamatani, Mitsuru Wakita, Shigeki Misawa, Atsushi Okuzawa, Takashi Miida, Toshio Naito, Kazuhisa Takahashi, Yoko Tabe
Summary: This study demonstrated the appropriate conditions for saliva sample collection, processing, and storage and indicated that the nCoV-DK is applicable for saliva samples, making the diagnostic method simple and safe.
CLINICAL LABORATORY
(2022)
Review
Chemistry, Analytical
Debayan Das, Manaswini Masetty, Aashish Priye
Summary: Paper-based diagnostics offer a cost-effective and portable alternative to traditional diagnostic methods. However, current paper-based serology tests lack the accuracy and sensitivity of molecular diagnostic tests. Loop-mediated isothermal amplification (LAMP) provides PCR-like performance while simplifying the assay complexity. This review discusses the integration of paper microfluidics with LAMP for improved accuracy and versatility in point-of-care testing.
Article
Medical Laboratory Technology
Firouze Hatami, Mohammad Mahdi Rabiei, Farid Javandoust Gharehbagh, Mohamad Amin Pourhoseingholi, Shahram Sabeti, Mahnaz Kheyrian, Ilad Alavi Darazam
Summary: This study describes the clinical utility of serial PCR testing in the final detection of COVID-19. The results indicate that serial PCR testing is unlikely to yield additional information.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Microbiology
Hosam M. Zowawi, Thamer H. Alenazi, Waleed S. AlOmaim, Ahmad Wazzan, Abdullah Alsufayan, Rihab A. Hasanain, Omar S. Aldibasi, Sahar Althawadi, Sarah A. Altamimi, Maysoon Mutabagani, Maha Alamri, Reem S. Almaghrabi, Hail M. Al-Abdely, Ziad A. Memish, Saleh A. Alqahtani
Summary: The study assessed the agreement and accuracy of the portable Biomeme SARS-CoV-2 system against the gold standard tests, which showed that it can rapidly and accurately generate test results within 80 minutes and is highly consistent with RT-PCR testing results, helping to provide rapid testing for patients in remote and low-resourced settings.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Sandhya Sharma, Emmanuel Thomas, Massimo Caputi, Waseem Asghar
Summary: HCV infections affect approximately 3% of the world population, requiring rapid and accurate diagnostic methods for effective disease management. A study presented an automated RT-LAMP-based molecular diagnostic set-up that can detect 500 virions/mL within 45 minutes, demonstrating high sensitivity and specificity.
Review
Chemistry, Multidisciplinary
Ofer I. Wilner, Doron Yesodi, Yossi Weizmann
Summary: The COVID-19 pandemic has highlighted the urgent need for quick, easy-to-use, reliable, and affordable diagnostic tests and devices at various locations. This paper introduces PCR and isothermal amplification reactions, reviews commercially available point-of-care nucleic acid diagnostic devices, and discusses efforts in developing reliable, portable, cost-effective, and automatic point-of-care nucleic acid diagnostic devices. Its purpose is to bridge the gap between academia and industry and share important knowledge on this topic.
Review
Immunology
Akanksha Roberts, Raghuraj Singh Chouhan, Deepshikha Shahdeo, Narlawar Sagar Shrikrishna, Veerbhan Kesarwani, Milena Horvat, Sonu Gandhi
Summary: A vaccine is crucial, but rapid and precise diagnostic methods are also essential in monitoring and combating the spread of SARS-CoV-2 and its variants. A variety of conventional and modern diagnostic methods have been developed, with ongoing research focusing on developing more economical tools to tackle the COVID-19 pandemic.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Medicine, Research & Experimental
Renata Salgado Fernandes, Juliana de Oliveira Silva, Karina Braga Gomes, Ricardo Bentes Azevedo, Danyelle M. Townsend, Adriano de Paula Sabino, Andre Luis Branco de Barros
Summary: The COVID-19 pandemic has fueled relentless exploration in diagnostic tests, treatments, and vaccines. RT-PCR remains the gold standard for diagnosis, but other molecular techniques and immunoassays have emerged. The development of rapid, accessible tests has led to the rise of point of care tests (POCT) that reduce pressure on screening and detection.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Plant Sciences
Ava N. Medberry, Ashish Srivastava, Alfredo Diaz-Lara, Maher Al Rwahnih, Dan E. V. Villamor, Ioannis E. Tzanetakis
Summary: Strawberry (Fragaria x ananassa) is a global important berry crop that is constantly threatened by viruses. A novel virus called strawberry virus 3 (StrV-3) was discovered, which does not show significant similarity with known rhabdoviruses, suggesting the establishment of a new genus. A triplex reverse-transcription PCR test was developed for detecting the virus in the National Clonal Germplasm Repository Fragaria collection. A CRISPR-Cas-based protocol was also developed for detecting the virus in candidate G1 plants. The potential of the strawberry aphid (Chaetosiphon fragaefolii) as a vector of the virus was evaluated, but with no success. This work enhances our knowledge of the Rhabdoviridae family and aids in the goal of releasing virus-free plant materials.
Article
Multidisciplinary Sciences
Amanda Rainey, Austin Pierce, Xiaoyun Deng, Luis A. Actis, Philip Smith, Andor J. Kiss, Timothy J. Wilson
Summary: The study validated a direct RT-PCR test on pooled saliva samples as an effective method of SARS-CoV-2 surveillance, with high accuracy and sensitivity. Preliminary results indicate that detecting viral RNA in saliva can serve as an efficient means for COVID-19 monitoring, especially in asymptomatic individuals.
Article
Public, Environmental & Occupational Health
Manaf Alqahtani, Abdulkarim Abdulrahman, Fathi Mustafa, Abdulla I. Alawadhi, Batool Alalawi, Saad I. Mallah
Summary: In addition to mass vaccination, early detection and isolation of infected cases is the best way to mitigate an outbreak. This study demonstrates the potential use of rapid antigen detection test (RADT) as a cost-effective and rapid method for detecting COVID-19.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Georgia Lamb, Joseph Heskin, Paul Randell, Nabeela Mughal, Luke S. P. Moore, Rachael Jones, Gary W. Davies, Michael Rayment
Summary: This study evaluated the performance of the Innova lateral flow immunoassay antigen device in real-world settings among hospital workers, showing a high positive predictive value during periods of high COVID-19 prevalence. LFD testing allows for earlier isolation of infected individuals and detection of symptomatic cases that may not qualify for PCR testing.
JOURNAL OF INFECTION
(2021)
Editorial Material
Oncology
P. Turcsanyi, E. Kriegova, L. Henzlova, L. Raida, R. Urbanova, T. Papajik
Article
Immunology
Gayane Manukyan, Tomas Papajik, Zuzana Mikulkova, Renata Urbanova, Veronika Smotkova Kraiczova, Jakub Savara, Milos Kudelka, Peter Turcsanyi, Eva Kriegova
JOURNAL OF IMMUNOLOGY RESEARCH
(2020)
Article
Oncology
Diana Malarikova, Adela Berkova, Ales Obr, Petra Blahovcova, Michael Svaton, Kristina Forsterova, Eva Kriegova, Eva Prihodova, Lenka Pavlistova, Anna Petrackova, Zuzana Zemanova, Marek Trneny, Pavel Klener
Article
Biochemistry & Molecular Biology
Gayane Manukyan, Anush Martirosyan, Ludek Slavik, Jana Ulehlova, Martin Dihel, Tomas Papajik, Eva Kriegova
Article
Biochemical Research Methods
Jakub Savara, Tomas Novosad, Petr Gajdos, Eva Kriegova
Summary: Recent studies have shown the potential of using long-read whole-genome sequencing (WGS) approaches and optical mapping (OM) for the detection of clinically relevant structural variants (SVs) in cancer research. Three main long-read WGS platforms are currently in use: Pacific Biosciences (PacBio), Oxford Nanopore Technologies (ONT) and 10x Genomics. This study compared optical maps of the breast cancer cell line SKBR3 with AnnotSV outputs from WGS platforms, and developed a software tool with comparative and filtering features. The majority of SVs detected by OM were confirmed by all WGS platforms within a certain distance threshold, while some types of SVs were only detected by specific platforms.
Article
Biochemical Research Methods
Michal Vasinek, Marek Behalek, Petr Gajdos, Regina Fillerova, Eva Kriegova
Summary: This article proposes a novel approach to modeling errors in the data obtained from the Bionano Genomics Inc. Saphyr system with Direct Label and Stain (DLS) chemistry. The main result is a framework for studying errors in the data obtained from the Saphyr instrument with DLS chemistry, using a simulation that computes how major sources of errors affect the distribution of fragment lengths in optical maps. The experiments show that this approach can be used to study errors in the optical mapping data analysis.
Article
Virology
Eva Kriegova, Regina Fillerova, Milan Raska, Jirina Manakova, Martin Dihel, Ondrej Janca, Pavel Sauer, Martina Klimkova, Petra Strakova, Petr Kvapil
Summary: The study highlights the importance of painless nasal-swab self-collection and direct RT-qPCR for the mass testing of SARS-CoV-2 during and after the pandemic.
Article
Microbiology
Petr Klempt, Ondrej Brzon, Martin Kasny, Katerina Kvapilova, Petr Hubacek, Ales Briksi, Matej Bezdicek, Vladimira Koudelakova, Martina Lengerova, Marian Hajduch, Pavel Drevinek, Sarka Pospisilova, Eva Kriegova, Milan Macek, Petr Kvapil
Summary: The study analyzed the distribution of SARS-CoV-2 genome variants in the Czech Republic during the first year of the pandemic, documenting the changing frequency of dominant variants and listing specific variants that could impact the diagnostic efficiency of RT-qPCR assays. The research also provided evidence of superinfection in several samples, potentially contributing to the high per capita numbers of COVID-19 cases and deaths during the initial year of the pandemic in the Czech Republic.
Article
Biochemistry & Molecular Biology
Petr Mlejnek, Petr Dolezel, Eva Kriegova, Nikola Pastvova
Summary: N-acetylcysteine (NAC) can induce ROS production in HL-60 and U937 cells, with cytotoxicity depending on the expression of ROS-modulating enzymes. In HL-60 cells, NAC induces cell death through NOX2, SOD1, SOD3, and MPO, while in U937 cells, lower expression levels of these enzymes lead to weaker cytotoxicity. The mode of cell death induced by NAC treatment in both cell lines is primarily apoptotic.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Gayane Manukyan, Zuzana Mikulkova, Peter Turcsanyi, Jakub Savara, Marketa Trajerova, Zuzana Kubova, Tomas Papajik, Eva Kriegova
Summary: This study demonstrates that differences in CLL cell size reflect their activation status, polarization, and migratory abilities, with large CLL cells being more activated and mobile than small CLL cells. This suggests the importance of cell-size heterogeneity within the CLL cell pool and the dynamics of cell-size changes for disease pathogenesis.
Article
Hematology
Anna Petrackova, Jakub Savara, Peter Turcsanyi, Petr Gajdos, Tomas Papajik, Eva Kriegova
Summary: Germline pathogenic ATM variants are associated with the risk and progression of chronic lymphocytic leukemia (CLL) patients. Most of these variants are pathogenic or predicted to be pathogenic, while a small number are benign. Patients with these pathogenic or predicted pathogenic ATM variants are more likely to develop the disease through gene inactivation, similar to patients with somatic ATM variants. Additionally, these variants are associated with reduced progression-free survival in CLL patients, comparable to the effects of ATM or TP53 gene disruptions.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Orthopedics
M. Trajerova, E. Kriegova, Z. Mikulkova, J. Savara, M. Kudelka, J. Gallo
Summary: This study identified four immune-phenotypes associated with different clinical trajectories in patients with knee osteoarthritis (KOA) based on the analysis of immune cells and protein patterns in synovial fluid. These findings contribute to a better understanding of the pathogenesis of KOA and provide new therapeutic targets.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Orthopedics
G. Manukyan, J. Gallo, Z. Mikulkova, M. Trajerova, J. Savara, Z. Slobodova, E. Fidler, B. Shrestha, E. Kriegova
Summary: This study compared the phenotypic and functional characteristics of neutrophils in synovial fluid derived from patients with knee osteoarthritis (KOA) and knee infection (INF), and found differences between the two groups. These findings suggest that neutrophils may play a significant role in the pathophysiology of KOA.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Virology
Milan Sova, Milos Kudelka, Milan Raska, Jan Mizera, Zuzana Mikulkova, Marketa Trajerova, Eliska Ochodkova, Samuel Genzor, Petr Jakubec, Alena Borikova, Ladislav Stepanek, Petr Kosztyu, Eva Kriegova
Summary: This study utilized a patient similarity network approach to uncover the relationships between immune and clinical factors in COVID-19 patients, providing insights into disease severity. Multivariate network approaches play a crucial role in analyzing complex datasets.