Article
Environmental Sciences
Eric N. Chiles, Ariana S. Huffmyer, Crawford Drury, Hollie M. Putnam, Debashish Bhattacharya, Xiaoyang Su
Summary: Corals form symbiotic relationships with dinoflagellate algae, bacteria, and other microbes, and nitrogen metabolism plays an important role in symbiotic regulation. Using stable isotope tracing, it was found that nitrogen assimilation in the coral holobiont may be compartmentalized, with different regions showing different nitrogen metabolism rates.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Pei-Chun Chou, Chih-Min Liu, Ching-Hui Weng, Kai-Chien Yang, Mei-Ling Cheng, Yuh-Charn Lin, Ruey-Bing Yang, Bai-Chuang Shyu, Song-Kun Shyue, Jin-Dian Liu, Shih-Pin Chen, Michael Hsiao, Yu-Feng Hu
Summary: This study reveals how fibroblasts drive the normal function of the sinoatrial node (SAN) by regulating metabolic reprogramming and rhythmic activity. The study also identifies the critical role of Aldoc in the interaction between fibroblasts and pacemaker cardiomyocytes.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Jia Lu, Wei Zhou, Fangfang Dou, Chenfei Wang, Zhihua Yu
Summary: Microglia energy metabolism is suppressed during chronic A beta-tolerant processes, but pharmacological activation of TRPV1 can rescue microglial dysfunction and restore immune responses, leading to reduced amyloid pathology and reversed memory deficits in AD mice. This study suggests that TRPV1 is an important target for regulating metabolic reprogramming in microglial functions for AD treatment.
Article
Biochemistry & Molecular Biology
Marissa D. Pokharel, David P. Marciano, Panfeng Fu, Maria Clara Franco, Hoshang Unwalla, Kim Tieu, Jeffrey R. Fineman, Ting Wang, Stephen M. Black
Summary: Mitochondria are vital for cell metabolism, growth, and function, and play a central role in endothelial cell dysfunction associated with various lung diseases, including PAH. Multiple pathways, such as nitric oxide signaling, glucose metabolism, fatty acid oxidation, and the TCA cycle, are dysregulated in PAH, leading to alterations in mitochondrial membrane potential, proliferation, and apoptosis. Further research is urgently needed, especially in endothelial cells, to fully characterize these pathways and develop effective treatments for PAH.
Article
Cell Biology
Danyang Chong, Yayun Gu, Tongyu Zhang, Yu Xu, Dandan Bu, Zhong Chen, Na Xu, Liangkui Li, Xiyu Zhu, Haiquan Wang, Yangqing Li, Feng Zheng, Dongjin Wang, Peng Li, Li Xu, Zhibin Hu, Chaojun Li
Summary: Ketone body plays a critical role in regulating postnatal heart development by promoting mitochondrial maturation and metabolic reprogramming.
Article
Food Science & Technology
Ning Zhang, Lin Li, Sen Li, Muhammad Akram Khan, Adnan Hassan Tahir, Muhammad Farhan Rahim, Ting Wang, Jiyu Zhao, Ruiyan Zhang
Summary: The neuroprotective effects of beta-hydroxybutyrate (BHB) were explored in a demyelination model called the cuprizone model. BHB was found to reduce demyelination and promote M2 microglia polarization. It also improved blood-brain barrier dysfunction and influenced the expression of various related genes. These findings suggest that BHB could be a potential therapeutic approach for preventing demyelinating diseases.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Biotechnology & Applied Microbiology
Roland C. Wilhelm, Samuel E. Barnett, Tami L. Swenson, Nicholas D. Youngblut, Chantal N. Koechli, Benjamin P. Bowen, Trent R. Northen, Daniel H. Buckley
Summary: Carbon metabolism in soil is poorly understood due to the difficulty in obtaining information about microbial metabolites produced by complex soil communities. Our study demonstrates the use of stable isotope probing (SIP) to track C-13 from supplied carbon sources into metabolite pools and biomass, providing valuable insights into carbon transformation in soils. Our findings show that the metabolism of different carbon sources influences the fate of carbon in soils, highlighting the importance of considering carbon source effects in soil metabolic studies. The use of SIP-metabolomics allows for the identification of metabolite markers of microbial growth, activity, and other aspects of microbial function in soil.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Qiushi Sun, Teresa W-M Fan, Andrew N. Lane, Richard M. Higashi
Summary: The metabolome is a complex network of enzyme-catalyzed reactions that require tracking metabolite substructures using stable isotopes. By utilizing UHR-MS and tandem MS, isotopic labels in metabolites can be identified, however, challenges lie in acquiring MS2 data due to the long acquisition time of chromatography-based UHR-MS1. A method using IC-UHR-MS1/DI-HR-MS2 has been developed to trace isotopologues in metabolites and acquire substructure label information simultaneously, facilitating the reconstruction of intersecting metabolic pathways and identifying enzyme-catalyzed reactions impacted by treatments.
ANALYTICAL CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Zheng-ping Huang, Shu-fen Liu, Jian-long Zhuang, Lin-yi Li, Mi-mi Li, Ya-li Huang, Yan-hong Chen, Xiang-rong Chen, Shu Lin, Li-chao Ye, Chun-nuan Chen
Summary: Parkinson's disease (PD) is an age-related neurodegenerative disorder characterized by damage to dopaminergic neurons. The specific pathogenesis of PD is still unknown, and current treatment methods have deficiencies. Microglial metabolic reprogramming plays a role in PD pathogenesis by affecting neuroinflammation and tissue repair. Strategies targeting specific metabolic pathways in microglia have shown potential for PD treatment.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Francesco Lasorsa, Nicola Antonio di Meo, Monica Rutigliano, Matteo Ferro, Daniela Terracciano, Octavian Sabin Tataru, Michele Battaglia, Pasquale Ditonno, Giuseppe Lucarelli
Summary: Prostate cancer is a common malignancy in men and a leading cause of cancer death. The cancer cells have specific metabolic characteristics, including increased lipogenesis, cholesterogenesis, and reliance on glutamine. The tumor microenvironment also plays a role in cancer progression through interactions between cancer and stromal cells. The use of multi-omic approaches can provide a comprehensive understanding of the metabolic changes in prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Ying Ma, Dongliang Li, Wenfeng Liu, Xiaoxiao Liu, Yingqi Xu, Xinrui Zhong, Fengnan Zhi, Xueling Jia, Yanan Jiang, Yuhua Fan
Summary: The study found that Resveratrol can partially exert a protective effect against atherosclerosis by modulating liver metabolism, alleviating atherosclerosis induced by a high-fat diet. The results also suggest that Resveratrol's impact on specific metabolic pathways may be beneficial in the treatment of atherosclerosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Won Dong Lee, Lingfan Liang, Jenna AbuSalim, Connor S. R. Jankowski, Laith Z. Samarah, Michael D. Neinast, Joshua D. Rabinowitz
Summary: The method of blood sample collection has significant effects on plasma metabolomics and stable isotope tracing in mice. Stress and sampling site both contribute to differences in the circulating metabolome. Lactate is the most important metabolite in unstressed mice metabolism and is produced in response to acute stress. Noninvasive arterial sampling can avoid these artifacts.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Jingjing Han, Qian Li, Yu Chen, Yonglin Yang
Summary: Metabolomic analysis is a powerful tool for monitoring dynamic changes in tumor metabolism and response to treatment. It can help identify early biomarkers of cancer development, predict individual responses to cancer drugs, assess drug efficacy, and monitor drug resistance. Stable isotope tracer technology is also utilized for studying tumor metabolism to track metabolite activity in the body and deep metabolic pathways.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Pharmacology & Pharmacy
Dayuan Wang, Xiao Gu, Xinyi Ma, Jun Chen, Qizhi Zhang, Zhihua Yu, Juan Li, Meng Hu, Xiaofang Tan, Yuyun Tang, Jianrong Xu, Minjun Xu, Qingxiang Song, Huahua Song, Gan Jiang, Zaiming Tang, Xiaoling Gao, Hongzhuan Chen
Summary: The polyphenol compound a-mangostin can reactivate the immune function of disease-associated microglia by reprogramming their metabolism, enhancing the degradation and phagocytosis of various misfolded proteins. Nanoformulated a-mangostin efficiently delivers the compound to microglia, relieving their reactive status and improving neuropathological changes in Alzheimer's and Parkinson's disease model mice. These findings provide evidence for rejuvenating microglial surveillance and suggest nanoformulated a-mangostin as a potential universal therapy for neurodegenerative diseases.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Microbiology
Chandrashekhar D. Patil, Rahul K. Suryawanshi, Divya Kapoor, Deepak Shukla
Summary: HSV-1 infection induces significant alterations in host metabolism, limiting the energy and macromolecular precursors required for viral replication. This study is of great importance for understanding the pathogenesis of HSV-1 infection.
Article
Biochemistry & Molecular Biology
C. Koufaris, G. N. Valbuena, Y. Pomyen, G. D. Tredwell, E. Nevedomskaya, C-H E. Lau, T. Yang, A. Benito, J. K. Ellis, H. C. Keun
Article
Oncology
Emily M. E. Barnes, Yitao Xu, Adrian Benito, Lili Herendi, Alexandros P. Siskos, Eric O. Aboagye, Anke Nijhuis, Hector C. Keun
BRITISH JOURNAL OF CANCER
(2020)
Article
Oncology
Marta Braga, Maciej Kaliszczak, Laurence Carroll, Zachary T. Schug, Kathrin Heinzmann, Nicoleta Baxan, Adrian Benito, Gabriel N. Valbuena, Stephen Stribbling, Alice Beckley, Gillian Mackay, Francesco Mauri, John Latigo, Chris Barnes, Hector Keun, Eyal Gottlieb, Eric O. Aboagye