Article
Food Science & Technology
Zixuan Xu, Zhenyang Hu, Zhilong Yu, Lijun Huang, Fangwei Yang, Yunfei Xie
Summary: Virtual screening targeting LuxS and LsrB proteins of Salmonella was used to identify 12 quorum sensing inhibitors (QSIs) applicable in the food industry. Among them, esculetin showed the lowest MIC and demonstrated significant inhibitory effects on Salmonella's motility, biofilm formation, extracellular polymer, and AI-2 signaling molecule production. Esculetin down-regulated the expression of genes related to quorum sensing and biofilm formation. This study highlights the potential of virtual screening and esculetin as a preservative in the food industry.
Article
Biochemistry & Molecular Biology
Aditi Vashistha, Nikhil Sharma, Yerramsetti Nanaji, Deepak Kumar, Gurpal Singh, Ravi P. Barnwal, Ashok Kumar Yadav
Summary: The overuse and inappropriate use of antibiotics has led to the development of drug resistant bacterial strains. Biofilm, a complex microorganism aggregation, plays a crucial role in infectious diseases. Efforts to disrupt biofilms have identified molecules that can inhibit quorum sensing, offering potential treatments for bacterial infections with lower dosages of medications.
BIOORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Feng-Wei Xia, Bing-Wei Guo, Yu Zhao, Jia-Li Wang, Yuan Chen, Xiu Pan, Xin Li, Jia-Xing Song, Yu Wan, Shun Feng, Ming-Yu Wu
Summary: This study develops a glycan-targeting photosensitizer, ACR-DMP, to fight against stubborn biofilms. ACR-DMP has high extracellular polymer penetrability and overcomes the hypoxic microenvironment, efficiently eradicating biofilms in vitro and in vivo. It not only changes membrane potential homeostasis and osmotic pressure balance, but also inhibits quorum sensing, two-component system, and efflux pump to conquer biofilm resistance.
ADVANCED MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Lujun Yin, Yingjie Wang, Shiliang Xiang, Kaizhong Xu, Bo Wang, Ai-Qun Jia
Summary: In this study, it was found that tyramine could inhibit the production of virulence factors in Burkholderia cenocepacia by blocking the quorum sensing systems. It was also shown to have low toxicity and potential as a combination therapy with traditional antibiotics.
BIOCHEMICAL PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Bhawandeep Kaur, Jeena Gupta, Sarika Sharma, Divakar Sharma, Sandeep Sharma
Summary: Staphylococcus aureus is a common pathogen causing various infections, with Methicillin-Resistant Staphylococcus aureus (MRSA) being particularly concerning. The primary antimicrobial resistance mechanisms in S. aureus involve efflux pumps and biofilm formation, with quorum sensing and drug efflux playing crucial roles. Efflux pump inhibitors (EPIs) have been identified as a promising approach to combat bacterial resistance, by inhibiting drug efflux mechanisms and transport of quorum sensing signalling molecules.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Infectious Diseases
Dinusha Chathurangi Rajapaksha, Shan Lakmal Edirisinghe, Chamilani Nikapitiya, Ilson Whang, Mahanama De Zoysa
Summary: In this study, it was found that the antimicrobial peptide octopromycin can inhibit the biofilm and quorum-sensing of Acinetobacter baumannii. Octopromycin reduced biofilm mass, caused a collapse in biofilm architecture, and successfully killed antibiotic-resistant persister cells. It also inhibited violacein production in Chromobacterium violaceum and alginate production, surface movements, and twitching motility of A. baumannii.
Review
Chemistry, Medicinal
Sudipta Paul Bhattacharya, Snigdha Karmakar, Kusumita Acharya, Arijit Bhattacharya
Summary: The development of biofilms is a protective strategy used by bacterial pathogens against the immune response and antimicrobial treatments. Quorum sensing, which alters gene expression profiles, has been identified as a key regulator of biofilm dynamics. With the rapid emergence of antimicrobial resistance, it is urgent to find alternative interventions to control biofilm infections. Exploring phytochemical products has shown promise in inhibiting quorum sensing and preventing biofilm formation. Recent studies have focused on triterpenoids and their derivatives, which have been found to disrupt quorum sensing and impair biofilm formation against bacterial pathogens.
Review
Microbiology
Bo Peng, Yanqun Li, Jiajia Yin, Wenping Ding, Wang Fazuo, Zhihui Xiao, Hao Yin
Summary: This study uses bibliometric analysis methods to explore the current status and trends in anti-QSA research, providing valuable insights for researchers to identify hot topics and explore new directions.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biology
Sirijan Santajit, Nitat Sookrung, Nitaya Indrawattana
Summary: Quorum sensing in ESKAPE bacteria is a global threat to public health, leading to increased antimicrobial resistance, virulence production, and biofilm formation, reducing treatment options. Understanding the mechanisms of quorum sensing can help predict underlying mechanisms of antimicrobial resistance and pathogenesis. This review discusses antibiotic resistance and pathogenesis caused by quorum sensing in ESKAPE pathogens, as well as strategies to control quorum sensing.
Review
Microbiology
Rohit Patel, Mansi Soni, Bilv Soyantar, Suruchi Shivangi, Swati Sutariya, Meenu Saraf, Dweipayan Goswami
Summary: The indiscriminate use of antibiotics has led to the emergence of drug-resistant strains. Many infectious diseases are caused by microbes that can communicate through quorum sensing (QS), and interfering with QS can effectively control their pathogenicity. Inhibition of QS has become a promising approach for developing new drugs, and various quorum sensing inhibitors (QSIs) have been identified. This review provides an overview of the QS mechanism, QS inhibition, and compounds with anti-QS potential, as well as the possibility of quorum sensing resistance.
ARCHIVES OF MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jyoti Yadav, Satyajeet Das, Divyapriya Karthikeyan, Ravneet Chug, Anupam Jyoti, Vijay Kumar Srivastava, Ajay Jain, Sanjit Kumar, Vinay Sharma, Sanket Kaushik
Summary: This review provides a comprehensive overview of the role of biofilm and quorum sensing in the emergence of multidrug-resistant strains of Enterococcus faecalis. The importance of protein drug targets involved in these processes is also emphasized.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Review
Pharmacology & Pharmacy
Mony Thakur, Yatin Khushboo, Yatin Kumar, Vinod Yadav, Avijit Pramanik, Kashyap Kumar Dubey
Summary: Pseudomonas aeruginosa has various virulence factors that contribute to host infection and persistence. These factors allow the bacteria to cause severe infections in immunocompromised individuals. P. aeruginosa is a nosocomial pathogen that is typically resistant to available antibiotics. Biofilm formation by P. aeruginosa is a major concern in hospitals and can lead to chronic infection and increased mortality risk. Therefore, it is crucial to develop new strategies to disrupt biofilm formation and improve antibiotic efficacy for the treatment of P. aeruginosa infections. Inhibition of quorum sensing and biofilm formation through pharmacological approaches, such as phytochemicals and essential oils, has shown promising results in laboratory studies. LasR, a regulatory protein, plays a key role in quorum sensing signaling, and designing an antagonist molecule that mimics the natural autoinducer could be the best approach for LasR inhibition. In this review, we discuss antibiotic resistance mechanisms and alternative approaches to combat the pathogenicity of P. aeruginosa.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Yijie Xu, Chunlan Zeng, Huiqi Wen, Qianqian Shi, Xu Zhao, Qingbin Meng, Xingzhou Li, Junhai Xiao
Summary: Several AI-2 QSIs targeting the LsrK/HPr PPI site have been discovered, providing potential for finding more effective AI-2 QSIs.
Article
Biotechnology & Applied Microbiology
R. Boominathan, Sandhanasamy Devanesan, Mohamad S. AlSalhi, A. Balasubramanian, Isam Zuhair Alkhalid, Parameswari Paul, A. J. A. Ranjit Singh
Summary: Cell-to-cell communication among Gram negative bacteria promotes biofilm formation over implants and leads to drug resistance. Quorum sensing interruption can inhibit biofilm formation and drug resistance. This study tested the quorum quenching action of marine red algae extract on biofilm forming bacteria and found positive results.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Pharmacology & Pharmacy
Giulia Bernabe, Giovanni Marzaro, Giuseppe Di Pietra, Ana Otero, Massimo Bellato, Anthony Pauletto, Melania Scarpa, Stefania Sut, Adriana Chilin, Stefano Dall'Acqua, Paola Brun, Ignazio Castagliuolo
Summary: In this study, a novel inhibitor of acyl-homoserine lactone (AHL)-mediated signaling in P. aeruginosa, GM-50, was identified. GM-50 reduced virulence factors, enhanced the anti-biofilm activity of aztreonam, and protected Galleria mellonella larvae from damage induced by P. aeruginosa. It also showed activity against clinical strains, highlighting its potential as a starting point for the development of new phenolic derivatives as QS inhibitors in P. aeruginosa infections.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sajjad Ahmad, Muhammad Sajjad, Hisham N. Altayb, Syed Sarim Imam, Sultan Alshehri, Mohammed M. Ghoneim, Saher Shahid, Muhammad Usman Mirza, Muhammad Shahid Nadeem, Imran Kazmi, Muhammad Waheed Akhtar
Summary: The carbohydrate-binding module (CBM3b) of the processive cellulase (CelO) from Clostridium thermocellum was found to enhance the catalytic activity and thermostability of the enzyme. The attachment of CBM3b at the N-terminus of the catalytic domain (GH5) increased the enzyme activity and stability.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Agronomy
Jawad Ul Haq, Muhammad Sharif, Waqas Ali Akbar, Hafeez Ur Rahim, Ishaq Ahmad Mian, Sajjad Ahmad, Juha M. Alatalo, Zaid Khan, Muhammad Mudassir
Summary: The application of arbuscular mycorrhiza fungi (AMF) infected roots of maize and single super phosphate (SSP) can improve nitrogen-phosphorus (NP) acquisition, yield, root infection activity, and AMF spore density in alkaline-calcareous soil. The combined use of AMF and SSP significantly improves yield-related parameters and plant nutrient uptake compared to sole SSP application. AMF inoculation also increases soil AMF spore density and root infection intensity, leading to potential increase in crop yield.
Article
Biochemistry & Molecular Biology
Muhammad Zubair, Sidra Khalil, Ijaz Rasul, Habibullah Nadeem, Fatima Noor, Sajjad Ahmad, Faris Alrumaihi, Khaled S. Allemailem, Ahmad Almatroudi, Faez Falah Alshehri, Zafer Saad Alshehri
Summary: Breast cancer is a silent killer disease among women and a significant economic burden. Despite advances in research and treatment, breast cancer remains a growing ailment. This study aims to identify new drug targets for breast cancer by targeting specific proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Taghreed N. Almanaa, Ayman Mubarak, Muhammad Sajjad, Asad Ullah, Muhammad Hassan, Yasir Waheed, Muhammad Irfan, Saifullah Khan, Sajjad Ahmad
Summary: Hantavirus, a member of the Bunyavirales order, is an emerging global pathogen that has affected millions of people worldwide. A potential multi-epitopes-based vaccine against hantavirus was designed using T-cell epitope prediction and immunoinformatics analysis. The vaccine's binding potential with TLR-3 immune receptor was evaluated, and docking studies revealed promising interactions. The findings suggest that the designed vaccine could be a potential candidate for protection against hantavirus.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Shaaban K. Mohamed, El Hassane Anouar, Sajjad Ahmad, Mohamed S. Abbady, Fatma K. Abdel-Wadood, Maha Q. M. Qahtan, Joel T. Mague, Youness El Bakri
Summary: In the search for a vaccine and treatment for COVID-19, scientists have synthesized a new triazolothiadiazine derivative and characterized its structure. Molecular docking analysis suggests that this compound has a good affinity for key enzymes of SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Asad Ullah, Mahboob ul Haq, Madiha Iqbal, Muhammad Irfan, Saifullah Khan, Riaz Muhammad, Amin Ullah, Muhammad Khurram, Metab Alharbi, Abdullah F. F. Alasmari, Sajjad Ahmad
Summary: Moraxella lacunata is an emerging gram-negative bacterium responsible for multiple nosocomial infections. A multi-epitope-based vaccine has been designed for M. lacunata using fusion of selected epitopes and cholera toxin-B adjuvant. Docking analysis and molecular dynamics simulation showed strong interactions and stable binding conformation between the vaccine and immune cell receptors. Theoretical binding free energies were estimated using MM-PBSA and MM-GBSA methods.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Athar Shafiq, Ujala Khalid, Umar Abdur Rehman, Eman Abdullah Almuqri, Maria Mudassir, Sajjad Ahmad, Muhammad Idrees Khan, Abbas Khan, Dong-Qing Wei
Summary: The global chaos caused by SARS-CoV-2 continues to worsen with the emergence of novel variants, which affect vaccine effectiveness, hACE2 attachment, and immune evasion. A new variant, B.1.640.2, with multiple spike protein mutations and deletions, has been reported in France and is spreading worldwide. Understanding the impact of these spike protein variations on host communication is crucial.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Infectious Diseases
Mohammad Riaz Khan, Sadiq Azam, Sajjad Ahmad, Qaisar Ali, Zainab Liaqat, Noor Rehman, Ibrar Khan, Metab Alharbi, Abdulrahman Alshammari
Summary: This study aimed to determine the antibiotic susceptibility pattern, molecular basis for antibiotic resistance, and mutations in selected genes of bacterial isolates. It was found that antibiotic resistance is increasing and requires attention for proper disease management.
Article
Infectious Diseases
Sonia Quddus, Zainab Liaqat, Sadiq Azam, Mahboob Ul Haq, Sajjad Ahmad, Metab Alharbi, Ibrar Khan
Summary: This study examined the expression of MexAB-OprM and MexCD-OprJ efflux pump genes in antibiotic-resistant Pseudomonas aeruginosa. A total of 200 samples were collected from two hospitals in Peshawar, Pakistan. The presence of antibiotic-resistant efflux pump genes was detected in 178 amoxicillin-clavulanic acid-resistant isolates, and mutations in these genes were found to potentially increase structural stability.
Article
Multidisciplinary Sciences
Youness El Bakri, Shaaban K. Mohamed, Kandasamy Saravanan, Sajjad Ahmad, Ahmed A. Mahmoud, Shaban A. A. Abdel-Raheem, Wael M. El-Sayed, Joel T. Mague, Souraya Goumri Said
Summary: In this study, the extraction and structure analysis of a natural product were performed. The conformational analysis of the compound's rings was investigated and confirmed experimentally and theoretically. The compound showed strong binding with the main protease enzyme of SARS-CoV-2, suggesting it as a potential lead structure against the virus.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Chemistry, Medicinal
Ali Irfan, Shah Faisal, Sajjad Ahmad, Sami A. Al-Hussain, Sadia Javed, Ameer Fawad Zahoor, Bushra Parveen, Magdi E. A. Zaki
Summary: In this study, the potential of sixteen furan-1,3,4-oxadiazole tethered N-phenylacetamide structural motifs as inhibitors of human tyrosinase (hTYR) and human tyrosinase-related protein-1 (hTYRP1) was evaluated using computational methods. The results showed that these structural motifs exhibited higher binding affinities towards hTYR and hTYRP1 than the standard inhibitor kojic acid. Furan-1,3,4-oxadiazoles BF4 and BF5 displayed the strongest binding affinities and showed stability in molecular dynamics simulations, indicating their potential as hTYRP1 and hTYR inhibitors against melanogenesis.
Article
Chemistry, Medicinal
Hizbullah Khan, Muhammad Sirajuddin, Amin Badshah, Sajjad Ahmad, Muhammad Bilal, Syed Muhammad Salman, Ian S. Butler, Tanveer A. Wani, Seema Zargar
Summary: One homoleptic and three heteroleptic palladium(II) complexes were synthesized and characterized. The antibacterial results showed that compound 1 had the highest activity among the screened compounds. Compound 3 exhibited the best affinity in the molecular docking study. In the anticancer evaluation, compound 2 showed the highest activity against the DU145 human prostate cancer cell line. The physicochemical parameters suggested that the evaluated compounds may be potential candidates for future antibiotics and anticancer agents.
Article
Public, Environmental & Occupational Health
Assad Hafeez, William James Dangel, Samuel M. Ostroff, Ayyaz Gul Kiani, Scott D. Glenn, Jaffar Abbas, Muhammad Sohail Afzal, Saira Afzal, Sajjad Ahmad, Ali Ahmed, Haroon Ahmed, Liaqat Ali, Muhammad Ali, Zahid Ali, Muhammad Arshad, Tahira Ashraf, Zulfiqar A. Bhutta, Sadia Bibi, Zahid A. Butt, Jai K. Das, Zehra Fadoo, Asif Hanif, Khezar Hayat, Ayesha Humayun, Khalid Iqbal, Usman Iqbal, Nauman Khalid, Ejaz Ahmad Khan, Muhammad Shahzeb Khan, Ahmad Azam Malik, Muhammad Naveed, Shumaila Naz, Robina Khan Niazi, Zahra Zahid Piracha, Umar Saeed, Muhammad Salman, Zainab Samad, Muhammad Arif Nadeem Saqib, Syed Mahboob Shah, Izza Shahid, Masood Ali Shaikh, Hina Shamshad, Kanwar Hamza Shuja, Muhammad Suleman, Anayat Ullah, Irfan Ullah, Saif Ullah, Sana Ullah, Yasir Waheed, Abdul Waris, Simon Hay, Christopher J. L. Murray, Ali H. Mokdad
Summary: The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2019 provides comprehensive estimates for Pakistan, highlighting improvements in life expectancy but persistent disparities. Key health risks include malnutrition, air pollution, high blood pressure, dietary risks, and tobacco consumption.
LANCET GLOBAL HEALTH
(2023)
Article
Biochemistry & Molecular Biology
Ameer Fawad Zahoor, Sadaf Saeed, Azhar Rasul, Razia Noreen, Ali Irfan, Sajjad Ahmad, Shah Faisal, Sami A. Al-Hussain, Muhammad Athar Saeed, Muhammed Tilahun Muhammed, Zeinab A. Muhammad, Magdi E. A. Zaki
Summary: Molecular hybridization has been successfully used to develop novel anticancer chemotherapeutic agents, and a series of indole-1,2,4-triazol-based compounds have shown potential as anti-Hep-G2 liver cancer drugs. Among them, the compound 8b containing a 3,4-dichlorophenyl moiety demonstrated excellent anti-Hep-G2 cancer chemotherapeutic efficacy.
Article
Biochemistry & Molecular Biology
Ali Irfan, Shah Faisal, Sajjad Ahmad, Muhammad Jawwad Saif, Ameer Fawad Zahoor, Samreen Gul Khan, Jamila Javid, Sami A. Al-Hussain, Muhammed Tilahun Muhammed, Magdi E. A. Zaki
Summary: In this study, benzofuran-appended 1,3,4-oxadiazole and 1,2,4-triazole compounds were screened and evaluated for their binding potential against HCV NS5B RNA-dependent RNA polymerase. The results showed that benzofuran-1,2,4-triazole compounds exhibited higher binding affinities and BF-9, BF-12, and BF-13 compounds showed excellent binding affinities.