Article
Engineering, Biomedical
Deepti Rana, Prasanna Padmanaban, Malin Becker, Fabian Stein, Jeroen Leijten, Bart Koopman, Jeroen Rouwkema
Summary: Given the need for control over evolving biochemical cues within synthetic matrices, this study focused on the incorporation of VEGF165 specific aptamers into cell-instructive polymer networks to regulate VEGF bioactivity. Bicomponent micropatterns were fabricated via photopatterning and demonstrated the retention of aptamers, molecular recognition, and VEGF sequestration. The study also revealed the ability of aptamer-tethered VEGF to spatiotemporally regulate vascular morphogenesis.
MATERIALS TODAY BIO
(2023)
Article
Materials Science, Biomaterials
Hui-Jie Zhang, Fu-Shu Li, Feng Wang, Han Wang, Tong-Chuan He, Russell R. Reid, Bai-Cheng He, Qingyou Xia
Summary: Silk sericin-based hydrogel, derived from transgenic platelet-derived growth factor (PDGF)-BB, effectively enhances osteogenesis and inhibits adipogenic differentiation. The cross-talk between Smad and Stat3 pathways is involved in the synergistic induction of osteogenic differentiation by BMP9 and PDGF-BB in mesenchymal stem cells (MSCs).
REGENERATIVE BIOMATERIALS
(2022)
Article
Engineering, Biomedical
Deepti Rana, Ajoy Kandar, Nasim Salehi-Nik, Ilyas Inci, Bart Koopman, Jeroen Rouwkema
Summary: This paper presents an aptamer-based programmable growth factor (GF) delivery platform that allows spatiotemporal control over vascular endothelial GF (VEGF) availability. The platform can sequester and release VEGF in a localized and time-dependent manner, and it has shown the ability to selectively guide cell responses and manipulate microvascular network formation within a 3D microenvironment.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Amir Mohammad Maadani, Erfan Salahinejad
Summary: Bioceramics, particularly calcium phosphates, bioactive glasses, and crystalline silicates, are widely used biomaterials for tissue regeneration and therapeutic delivery. Polymeric coatings, such as polylactic acid (PLA) and poly(lactic-co-glycolic acid) (PLGA), have been extensively researched for enhancing the properties of ceramic tissue engineering scaffolds. Both PLA and PLGA can improve the mechanical behaviors of the scaffolds, but PLA has a greater influence on their biodegradability, bioactivity, and delivery kinetics. However, the biocompatibility response to this surface treatment is dependent on the specific conditions and needs further experimentation for accurate determination.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Biochemistry & Molecular Biology
Delfo D'Alessandro, Claudio Ricci, Mario Milazzo, Giovanna Strangis, Francesca Forli, Gabriele Buda, Mario Petrini, Stefano Berrettini, Mohammed Jasim Uddin, Serena Danti, Paolo Parchi
Summary: The demand for bone substitutes is rising in Western countries, with new strategies needed to promote bone and blood vessel generation within scaffolds. Piezoelectricity has been shown to be a stimulating signal for bone and vascular tissue regeneration, offering potential in developing successful vascularized bone replacements.
Article
Engineering, Biomedical
Caroline D. Hoemann, Javier Rodriguez Gonzalez, Jessica Guzman-Morales, Gaoping Chen, Ebrahim Jalali Dil, Basil D. Favis
Summary: This study found that non-inflammatory chitosan coatings promoted cartilage infiltration into and over a bioplastic scaffold, and were compatible with trabecular bone integration. Compared to drill-only defects, the 83-99-PCL scaffold induced angiogenesis and slight bone resorption, with the smallest change in bone volume fraction.
BIOACTIVE MATERIALS
(2022)
Article
Cell Biology
Marion Mesnieres, Anna-Marei Bohm, Nicolas Peredo, Dana Trompet, Roger Valle-Tenney, Manmohan Bajaj, Nikky Corthout, Elena Nefyodova, Ruben Cardoen, Pieter Baatsen, Sebastian Munck, Andras Nagy, Jody J. Haigh, Satish Khurana, Catherine M. Verfaillie, Christa Maes
Summary: The balanced local crosstalk within the skeletal niche is crucial for the successful settlement of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow, with overproduction of VEGF by osteoprogenitor cells impacting stromal-vessel interface and vascular architecture, resulting in impaired HSPC homing and survival. These findings highlight potential targets for improving stem cell therapies, as similar osteo-angiogenic niche changes are induced by irradiation in adult mice.
Article
Biophysics
Upasana Ghimire, Rupesh Kandel, Sita Shrestha, Joon Yeon Moon, Se Rim Jang, Bishnu Kumar Shrestha, Chan Hee Park, Cheol Sang Kim
Summary: Fabricating bioartificial bone graft ceramics with properties similar to native stem-cell niches is challenging. Infections caused by microbial invasion are also a concern. In this study, biomimetic porous scaffolds were developed, which significantly improved biocompatibility, osteo-regeneration, mechanical strength, and antibacterial properties. These scaffolds showed superior performance compared to conventional scaffolds, making them promising for bone tissue regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Prashant Jain, Chethan D. Shanthamurthy, Shani Leviatan Ben-Arye, Sharon Yehuda, Sharvani S. Nandikol, Hirekodathakallu Thulasiram, Vered Padler-Karavani, Raghavendra Kikkeri
Summary: A potential heparan sulfate (HS) ligand was discovered to target several growth factors using specific HS tetrasaccharide ligands. One tetrasaccharide ligand showed strong binding with the VEGF(165) growth factor, and in vitro experiments demonstrated the therapeutic potential of these synthetic HS ligands by inhibiting vascular endothelial cell proliferation, migration, and angiogenesis in the presence of VEGF(165) and the specific tetrasaccharides.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Article
Chemistry, Multidisciplinary
Yang Liu, Linyu Long, Fanjun Zhang, Xuefeng Hu, Jieyu Zhang, Cheng Hu, Yunbing Wang, Jianguo Xu
Summary: The study introduced a GelMA microneedle platform for sustained and controlled local delivery of AAV-VEGF, which achieved homogeneous distribution and high transfection efficiency in ischemic brains. Results showed that MNs loaded with AAV-VEGF increased VEGF expression in the brain cortex and enhanced functional angiogenesis and neurogenesis.
JOURNAL OF CONTROLLED RELEASE
(2021)
Review
Engineering, Biomedical
Peng Wang, Yonghui Yuan, Ke Xu, Hongshan Zhong, Yinghui Yang, Shiyu Jin, Ke Yang, Xun Qi
Summary: Copper is an essential trace element in the human body, impacting health through enzyme and protein functions. Researchers are increasingly focusing on developing novel copper-containing biomaterials with unique properties for cardiovascular protection, bone healing, and antibacterial effects. These materials also show potential in wound healing, cancer treatment, PET imaging, and radiotherapy applications.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Jaidev L. Chakka, Timothy Acri, Noah Z. Laird, Ling Zhong, Kyungsup Shin, Satheesh Elangovan, Aliasger K. Salem
Summary: This study utilized 3D printing to create a polylactic acid scaffold that, after surface functionalization, promoted blood vessel formation and bone growth. The scaffold showed potential for bone defect repair in both in vitro and in vivo settings.
Article
Clinical Neurology
Carlos Escudero, Jesenia Acurio, Eduardo Lopez, Andres Rodriguez, Antonia Benavente, Evelyn Lara, Steven J. Korzeniewski
Summary: Circulating VEGF levels were found to be elevated 24-48 hours after acute ischemic stroke and were associated with prognosis of moderate to severe disability at 6 months. Combining VEGF with interleukin 6 and C-reactive protein significantly improved predictive performance compared to using VEGF alone.
EUROPEAN JOURNAL OF NEUROLOGY
(2021)
Article
Multidisciplinary Sciences
Zhiyang Li, Heng Zhang, Yujie Sun, Zhuangzhuang Feng, Bijia Cui, Jingxia Han, Yinan Li, Huijuan Liu, Tao Sun
Summary: Angiogenesis plays a crucial role in embryologic development, growth, and tissue repair, but dysregulation of this process is a hallmark of various pathologies, including cancer. Existing methods for evaluating cell vascular formation are often limited to static analysis, leading to bias. Researchers have developed code scripts to study the dynamic angiogenesis process, allowing for the screening of drugs that can affect various parameters of vascular formation. Animal experiments have confirmed the efficacy of these drugs in inhibiting blood vessel formation, providing a new perspective for angiogenesis research and drug development.
Article
Materials Science, Multidisciplinary
Haiyue Zu, Kelvin Chau, Temitope Olumide Olugbade, Lulu Pan, Chris Halling Dreyer, Dick Ho-Kiu Chow, Le Huang, Lizhen Zheng, Wenxue Tong, Xu Li, Ziyi Chen, Xuan He, Ri Zhang, Jie Mi, Ye Li, Bingyang Dai, Jiali Wang, Jiankun Xu, Kevin Liu, Jian Lu, Ling Qin
Summary: This study compared the microstructure, mechanical performance, corrosion behavior, and biocompatibility of magnesium hip stents fabricated via different methods. Results showed that machining stents outperformed molding stents, and corrosion resistance and biocompatibility could be improved by paraffin-filling.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chris H. Dreyer, Niklas R. Jorgensen, Soren Overgaard, Ling Qin, Ming Ding
Summary: This study demonstrates that MSCs and VEGF can promote bone regeneration in sheep to a level comparable to allograft, with similar mechanical fixation and without visible side effects or systemic increase in bone biomarkers.
BIOMED RESEARCH INTERNATIONAL
(2021)
Article
Endocrinology & Metabolism
Ming Ding, Soren Overgaard
Summary: This study compared microarchitectural and mechanical properties of rheumatoid arthritis (RA), osteoarthritis (OA), osteoporosis (OP), and ageing controls in cancellous bones. While global morphometry showed no significant differences between diseased bones, local morphometry revealed greater plate density in the RA group. Ultimate stresses in the diseased groups were significantly lower than in the control group, indicating more severe degeneration and reduced bone strength in these conditions.
CALCIFIED TISSUE INTERNATIONAL
(2022)
Article
Biology
Jonas Hakansson, Weili Jiang, Qian Xue, Xudong Zheng, Ming Ding, Anurag A. Agarwal, Coen P. H. Elemans
Summary: The study identifies wall impingement as the aerodynamic mechanism of USV production in rats and mice, with a motor control model predicting motor gesture trajectories of USV call types. The results suggest that changes in USV production may be influenced by both altered motor programs and laryngeal geometry. This work provides a neuromechanical framework to evaluate the contributions of brain and body in shaping USVs and linking descending motor control to USV production.
Article
Biochemistry & Molecular Biology
Natasja Leth Bergholt, Ari Demirel, Michael Pedersen, Ming Ding, Tue Wenzel Kragstrup, Thomas Andersen, Bent Winding Deleuran, Casper Bindzus Foldager
Summary: Systemic Intermittent Hypoxic Therapy (IHT) can inhibit osteoclastic activity and prevent the resorption of allogenic bone grafts by activating multiple pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Orthopedics
Kristian Kjaergaard, Claus Varnum, Ming Ding, Soren Overgaard
Summary: This nationwide population-based study investigated the safety of vitamin E-doped cross-linked polyethylene (VEPE) liners compared to other types of polyethylene in total hip arthroplasty (THA). The results showed that VEPE liners had a lower risk of revision for polyethylene-related issues compared to cross-linked liners (XLPE), but had a higher risk of all-cause revision within 3 months.
JOURNAL OF ARTHROPLASTY
(2022)
Article
Engineering, Biomedical
Mads Suhr Nielsen, Maria Dalgaard Mikkelsen, Signe Helle Ptak, Eva Kildall Hejbol, Julia Ohmes, Thuan Nguyen Thi, Vy Tran Nguyen Ha, Xavier Frette, Sabine Fuchs, Anne Meyer, Henrik Daa Schroder, Ming Ding
Summary: The study investigated the efficacy of low-molecular weight marine bioactive compound fucoidan on bone regeneration, comparing it to allograft in sheep. Results showed that while all groups exhibited adequate bone formation, the allograft group had higher bone volume fractions. However, the combination of HA and FUC showed similar fixation strength to allograft, indicating potential as a substitute in bone graft surgeries.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Chemistry, Applied
Julia Ohmes, Maria Dalgaard Mikkelsen, Thuan Thi Nguyen, Vy Ha Nguyen Tran, Sebastian Meier, Mads Suhr Nielsen, Ming Ding, Andreas Seekamp, Anne S. Meyer, Sabine Fuchs
Summary: Fucoidans, polysaccharides found in brown macroalgae, have multiple bioactivities important for bone regeneration and bone health. However, their medical applications are limited due to their chemical heterogeneity and unclear structure-function relationships. Innovations in extraction techniques and post processing steps are needed to produce homogeneous fucoidan molecules with customized bioactivities.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Biomedical
Ming Ding, Kariatta Esther Koroma, David Wendt, Ivan Martin, Roberta Martinetti, Stig Jespersen, Henrik Daa Schroder, Soren Overgaard
Summary: This study investigates the efficacy of a carbonated hydroxyapatite (CHA) with/without bone marrow nuclear cells (BMNCs) on spine fusion rate and fusion mass microarchitecture using a bioreactor. The results indicate that the bioreactor can activate the substitute containing BMNCs and achieve comparable results in spine fusion.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Endocrinology & Metabolism
Ankita Agrawal, Maria Ellegaard, Kristian Agmund Haanes, Ning Wang, Alison Gartland, Ming Ding, Helle Praetorius, Niklas Rye Jorgensen
Summary: The increased incidence of bone fractures in the elderly is associated with gradual sarcopenia. The study found that the P2Y(2) receptor has no role in the bone loss after a period of skeletal unloading and bone recovery after restoration of muscle function in mice.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Fredrik K. E. Guldbaek-Svensson, Mathias M. M. Thygesen, Teresa H. H. Nielsen, Trine W. W. Mikkelsen, Ming Ding, Michael Pedersen, Henrik Lauridsen
Summary: A newly developed ultrasound-based technique is applied to visualize and quantify neovascularization in the regenerating spinal cord of a regeneration competent animal model, the Mexican axolotl. The results show that the ultrasound-based angiographic procedure is applicable to visualize and quantify neovascularization in a noninvasive and harmless fashion in longitudinal experiments.
CONTRAST MEDIA & MOLECULAR IMAGING
(2023)
Article
Multidisciplinary Sciences
Li Chen, Kaikai Shi, Nicholas Ditzel, Weimin Qiu, Florence Figeac, Louise Himmelstrup Dreyer Nielsen, Michaela Tencerova, Justyna Magdalena Kowal, Ming Ding, Christina Moller Andreasen, Thomas Levin Andersen, Moustapha Kassem
Summary: The levels of KIAA1199, a factor secreted by bone marrow stromal cells, are associated with the risk of osteoporotic fracture. KIAA1199 deficiency enhances bone formation, accelerates bone healing, and protects against ovariectomy-induced bone loss. Transplanted skeletal stem cells generate secreted factors that regulate bone regeneration, and KIAA1199 is identified as one of these factors. Patients with higher plasma levels of KIAA1199 have a higher risk of osteoporotic fracture, while lower expression levels of KIAA1199 in patient bone marrow stromal cells are associated with reduced osteogenic differentiation potential. In vitro and in vivo experiments show that KIAA1199-deficient bone marrow stromal cells exhibit enhanced osteoblast differentiation and ectopic bone formation. KIAA1199 knockout mice have increased bone mass, biomechanical strength, bone formation rate, and accelerated healing of surgically generated bone defects, and are protected from ovariectomy-induced bone loss. Mechanistically, KIAA1199 regulates osteogenesis by inhibiting the production of osteopontin by osteoblasts through integrin-mediated AKT and ERK-MAPK signaling pathways. Therefore, KIAA1199 is a regulator of osteoblast differentiation and bone regeneration, and could be a potential target for treating or managing low bone mass conditions.
NATURE COMMUNICATIONS
(2023)
Article
Orthopedics
J. A. N. El-Sahoury, K. Kjaergaard, O. Ovesen, C. Hofbauer, S. Overgaard, M. Ding
Summary: This study aimed to investigate the differences in wear between cross-linked vitamin E-diffused polyethylene (vE-PE) and cross-linked polyethylene (XLPE) acetabular component liners and between 32 and 36 mm head sizes at the ten year follow up. The results showed that the wear of vE-PE acetabular liners was significantly lower than that of XLPE acetabular liners.
BONE & JOINT JOURNAL
(2023)