Article
Chemistry, Analytical
Ryoutarou Oishi, Kaho Maki, Tatsumi Mizuta, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto
Summary: An enzyme-responsive fluorescent nanoemulsion based on lipophilic dye liquid was developed for alkaline phosphatase detection, showing higher sensitivity and shorter response time compared to conventional NE.
Article
Pharmacology & Pharmacy
Federica Foglietta, Annalisa Bozza, Chiara Ferraris, Luigi Cangemi, Valentina Bordano, Loredana Serpe, Katia Martina, Loretta Lazzarato, Stefania Pizzimenti, Margherita Grattarola, Marie Angele Cucci, Chiara Dianzani, Luigi Battaglia
Summary: Despite advancements in cancer genomic and immunotherapies, advanced melanoma remains a life-threatening disease. In order to improve targeted drug delivery to the tumor, injectable lipid nanoemulsions were functionalized with proteins using two different approaches: chemically grafting transferrin for active targeting, and wrapping cancer cell membrane fragments for homotypic targeting. Protein functionalization was successful in both cases. Preliminary studies using flow cytometry showed that cell-membrane-fragment-wrapped nanoemulsions had higher cell uptake compared to uncoated nanoemulsions. However, transferrin grafting had a less pronounced effect in serum-enriched medium, possibly due to competition with endogenous proteins. Moreover, a more significant internalization was achieved when a pegylated heterodimer was used for conjugation (p < 0.05).
Review
Biochemical Research Methods
Liam Adair, Elizabeth J. New
Summary: Small-molecule fluorophores are versatile tools for studying cellular architecture and biological processes. However, their application in vivo faces challenges such as biocompatibility, chemostability, photostability, and light penetration. Recent advances in designing fluorophores with improved properties and functionalization with targeting and sensing groups have allowed the development of in vivo fluorogenic, ratiometric, and reversible sensors.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Materials Science, Ceramics
Nadja Klippel, Gregor Jung, Guido Kickelbick
Summary: This study investigated the incorporation mechanisms of fluorescent dyes in silica nanoparticles and found that covalent bonding is necessary for fluorescence stability. The use of protective layers can reduce dye leaching.
JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xing Wei, Hongxing Zhang, Yuanqiang Sun, Jing Liu, Zhaohui Li
Summary: A lipid droplet targeting fluorescent probe with a large Stokes shift was successfully achieved by rotating ester groups, showing promising potential for specific imaging applications.
Article
Biochemistry & Molecular Biology
Getnet Midekessa, Kasun Godakumara, Keerthie Dissanayake, Mohammad Mehedi Hasan, Qurat Ul Ain Reshi, Toonika Rinken, Alireza Fazeli
Summary: Research has shown that the concentration of CMG fluorescent dye affects the size of fluorescent nanoparticles (fl-NPs), with an increase in dye concentration resulting in a significant decrease in size. Zeta potential (ZP) values of fl-NPs from JAr cells also shift significantly towards more negative or less negative values with varying dye concentrations. Furthermore, the proportion of CMG labeling of NPs varies across EVs purified using different methods and from different biological sources.
Article
Chemistry, Applied
Laura Espinar-Barranco, Jose Manuel Paredes, Angel Orte, Luis Crovetto, Emilio Garcia-Fernandez
Summary: In this study, a sensor with solvatofluorochromic property was used to investigate the initial stages of beta-amyloid peptide aggregation in Alzheimer's disease. By detecting changes in environmental polarity, the hydrophobicity of the aggregates can be quantified and different types of aggregates can be distinguished using advanced fluorescence spectroscopy and imaging techniques.
Article
Cell Biology
Chen Chen, Niangui Cai, Qian Niu, Ye Tian, Yunyun Hu, Xiaomei Yan
Summary: This study systematically characterized the performance of lipophilic membrane dyes (LMDs) or probes (LMPs) in labeling extracellular vesicles (EVs). The study found that PKH67 and PKH26 could effectively label EVs, while excessive PKH26 could damage the structure of EVs. Di-8-ANEPPS and high concentration of DiI achieved efficient and uniform labeling of EVs. The study also discovered that LMDs can aggregate and form micelles, making it difficult to differentiate them from labeled EVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Chemistry, Analytical
Peisheng Tang, Qing Wang, Qiuyan Tan, Kun Huang, Bingxin Du, Lijuan Liang
Summary: In this study, a terpyridyl functionalized rhodamine dye (TRMe) was designed and prepared, which selectively enhances fluorescence response to viscosity in water-glycerol mixtures and can respond to viscosity enhancement over a wide pH range. Furthermore, TRMe demonstrates excellent mitochondria-targeted performance and can be used as a tracer for viscosity imaging in living cells.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Tanmay Vyas, Manashjit Gogoi, Abhijeet Joshi
Summary: In this study, a pH-sensitive CQD thin film was developed for the detection of textile dye pollutants in water resources. The film exhibited fast and accurate detection of various acidic and basic dyes, with low detection limits.
Article
Spectroscopy
Zixuan Yang, Yaqiang Tian, Sheng Cang, Leiyu Zhang, Liang Liu
Summary: This study investigated four carbazole-modified fluorescent emitters, highlighting the superior emission performance of benzo-thiazole-based dyes under the presence of metal cations. A fluorescent dye with a linear working curve and good selectivity was studied.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Yuzhen Zhao, Chaonian Li, Tingting Lang, Yitian Sun, Qingbo Li, Xinli Shi, Ruijuan Yao, Huimin Zhang, Yang Zhao
Summary: Brightening polymer-stabilized bistable cholesteric liquid crystal (PSBCLC) films with doped fluorescent dyes were prepared using the polymerization-induced phase separation (PIPS) method. The transmittance performance behavior of these films in both states (focal conic and planar) and absorbance change in multiple dye concentrations were studied. Dye dispersion morphology with different concentrations was obtained. The optimal concentration of dye-doped PSBCLC films with high contrast ratio and low drive voltage was found, which is expected to have great potential applications in cholesteric liquid crystal reflective displays.
Article
Chemistry, Organic
Rabi Sankar Das, Ayan Mukherjee, Samiran Kar, Tapas Bera, Shreya Das, Arunima Sengupta, Samit Guha
Summary: This article describes a novel red fluorescent mechanically interlocked molecule with dual targeting functionality for live cancer cell specific active targeting and imaging of malignant lysosomes.
Article
Chemistry, Multidisciplinary
Zijing Li, Bin Huang, Yuan Wang, Wenbo Yuan, Yijing Wu, Ruitao Yu, Guichuan Xing, Taotao Zou, Youtian Tao
Summary: 2RDNTPA, a novel acceptor-donor-acceptor type molecule, demonstrates excellent optical properties suitable for fluorescence imaging of living cancer cells.
Article
Chemistry, Multidisciplinary
Tian-Bing Ren, Zhi-Yao Wang, Zhen Xiang, Peng Lu, Huan-Hua Lai, Lin Yuan, Xiao-Bing Zhang, Weihong Tan
Summary: Organic dye based NIR-II fluorescent probes offer high signal-to-background ratio and deep-tissue imaging capabilities. A novel class of polymethine dyes, NIRII-RTs, with bright and stable NIR-II emission and large Stokes shifts have been designed. These dyes, particularly NIRII-RT3 and NIRII-RT4, can serve as effective platforms for activatable bioimaging probes. Target-activatable NIRII-RT probes for pH, ATP, and metal-ion detection were synthesized, demonstrating real-time monitoring of drug-induced hepatotoxicity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Multidisciplinary
Andrey S. Klymchenko
Summary: Biomembranes, as lipid structures, play a crucial role in cellular processes. The development of chemical tools, such as fluorescent probes, for sensing and imaging biomembranes is a rapidly growing field. This Account focuses on advances in smart molecules for membrane targeting and sensing, with a particular emphasis on plasma membranes and organelles.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Lazare Saladin, Ophelie Dal Pra, Andrey S. S. Klymchenko, Pascal Didier, Mayeul Collot
Summary: Dual-emissive photoconvertible fluorophores (DPCFs) are useful for tracking labeled cells in bioimaging. The directed photooxidation-induced conversion (DPIC) mechanism allows efficient DPCFs to be obtained by conjugating a coumarin to aromatic singlet-oxygen reactive moieties (ASORMs). Pyrrole is a suitable ASORM that provides a high hypsochromic shift and fast conversion. By modifying pyrrole, we can tune the photoconversion properties of the DPCFs, leading to an improved dual emissive converter, SCP-Boc, with high brightness and a 63% enhanced photoconversion yield. SCP-Boc was successfully used for sequential cell photoconversion using laser scanning confocal microscopy.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Pharmacology & Pharmacy
Xinyue Wang, Halina Anton, Thierry Vandamme, Nicolas Anton
Summary: This review provides an updated insight into the characterization methods of nano-emulsions, including new and unconventional experimental approaches. It offers an overview of various techniques used to characterize nano-emulsions, ranging from classical methods to in vitro, ex vivo, and in vivo evaluation. The review also introduces innovative methods such as nanoparticle tracking analysis, small-angle X-ray scattering, Raman spectroscopy, and nuclear magnetic resonance. The use of lipophilic fluorescent probes and imaging techniques to understand the stability, surface decoration, release mechanisms, and in vivo fate of nano-emulsion droplets is discussed.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Article
Chemistry, Physical
Germain A. Brou, Gildas K. Gbassi, Ievgen Shulov, Aidar Seralin, Andrey S. Klymchenko, Thierry F. Vandamme, Nicolas Anton
Summary: In this study, we investigated the characterization and tracking of chemical reactions of amphiphile compounds on model interfaces. By using a custom-made surfactant and interfacial crosslinking through click chemistry, we observed changes in monolayer properties and dilatational elasticity. Through bulk phase exchange and washing, we specifically measured the interfacial effects of the entities adsorbed and trapped at the interface.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Nanoscience & Nanotechnology
Fei Liu, Nicolas Anton, Yosuke Niko, Andrey S. Klymchenko
Summary: Current biomedical applications of nanocarriers focus on controlled drug delivery, but this study proposes a different approach where toxic molecules are extracted from biological tissues using lipid nanoemulsion (NE) droplets. By designing a highly lipophilic amine (LipoAmine), cargo-aldehydes can be reacted and encapsulated inside lipid NEs through dynamic covalent chemistry. The formation of imine conjugates was validated and the NEs successfully extracted fluorescent dye and toxic aldehydes from cells, demonstrating the potential for developing detoxification platforms.
ACS APPLIED BIO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Deep Sekhar Biswas, Paraskevi Gaki, Elisabete Cruz Da Silva, Antoine Combes, Andreas Reisch, Pascal Didier, Andrey S. Klymchenko
Summary: By studying the FRET phenomenon between fluorescent organic nanoparticles, it is found that the efficiency does not follow the Forster law and can achieve high efficiency at long distances. This opens up the possibility of using advanced optical nanomaterials for amplified FRET-based biosensing.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Reimi Pelletier, Dmytro I. Danylchuk, Hela Benaissa, Fanny Broch, Romain Vauchelles, Arnaud Gautier, Andrey S. Klymchenko
Summary: Genetically encoded protein labels combined with polarity-sensitive fluorescent probes provide information about the nanoscale environments of proteins within subcellular compartments. A solvatochromic HaloTag probe, NR12-Halo, specifically labels a variety of proteins localized in different cell compartments and distinguishes proteins within lipid membranes. This probe also reveals changes in protein environments during their life cycle, heterogeneity in membrane protein polarity, and the effects of mechanical stress and fatty acid diet on protein nanoenvironments. The solvatochromic HaloTag probe is a promising tool for studying protein interactions and nanoscale environments within subcellular structures.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Chemistry, Multidisciplinary
L. Saladin, O. Dal Pra, A. S. S. Klymchenko, P. Didier, M. Collot
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Applied
Malik Muhammad Irfan, Shefaat Ullah Shah, Kifayat Ullah Shah, Nicolas Anton, Ysia Idoux-Gillet, Guillaume Conzatti, Elise Perennes, Thierry Vandamme
Summary: The purpose of this study was to develop a stable lyophilised finasteride nanosystem (FNS-NS) for topical delivery. The FNS-NS was fabricated using ultrasonication and the impact of different cryoprotectants on its characteristics was investigated. The lyophilised FNS-NS showed spherical shape, a particle size range of 188.6 nm +/- 4.4 to 298.7 nm +/- 4.7, low PDI values, and zeta potential within -38.3 to +53.3 mV. Confocal laser microscopy demonstrated higher cellular internalisation for undecorated FNS-NS compared to its chitosan-decorated counterpart. The lyophilised FNS-NS remained stable for 90 days and the FNS-NS with 15% trehalose showed promising attributes for topical delivery in treating androgenic alopecia.
JOURNAL OF MICROENCAPSULATION
(2023)
Review
Engineering, Biomedical
Tyler C. Owens, Nicolas Anton, Mohamed F. Attia
Summary: Computed tomography (CT) is a powerful imaging technique widely used in modern medicine, but it often requires the use of contrast agents to visualize structures with similar densities. Current clinical contrast agents for CT have significant drawbacks, such as nephrotoxicity and short circulation half-lives. Nanoparticle contrast agents have shown promise and are likely to be the main component of the next generation of CT contrast agents.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Deep Sekhar Biswas, Nina Melnychuk, Caterina Severi, Pascal Didier, Andrey S. Klymchenko
Summary: Light-harvesting is a fundamental process in nature that drives the development of artificial systems for various applications. Researchers have discovered that using blank hydrophobic salts can suppress dye aggregation within nanoparticles, improving their fluorescence properties and enabling efficient energy transfer and antenna effects.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Lucie Haye, Nour Fayad, Richard C. Knighton, Antoine Combes, Olivier Jeannin, Andrey Klymchenko, Thibault Gallavardin, Niko Hildebrandt, Loic J. Charbonniere, Andreas Reisch
Summary: This study investigates the physicochemical and photophysical properties of Re diimine complexes and Cy5 dyes coencapsulated in polymer nanoparticles (NPs), and their compatibility for bioimaging. The results show that encapsulation significantly enhances the photoluminescence brightness and lifetimes of Re complexes, while coencapsulation of Cy5 leads to bright near-infrared emission upon Re complex excitation. Furthermore, the Re-Cy5 NPs exhibit high-contrast photoluminescence imaging in mammalian cells.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Chemistry, Multidisciplinary
Anila Hoskere Ashoka, Ilya O. Aparin, Andreas Reisch, Andrey S. Klymchenko
Summary: Brightness is crucial for (bio)molecular detection and optical bioimaging. This tutorial review provides definitions of brightness and describes the major approaches to its analysis. It also discusses the current chemical methods to combat Aggregation-Caused Quenching and compares the brightness and other properties of different fluorescent organic nanoparticles. The importance of brightness in biological applications is also analyzed.
CHEMICAL SOCIETY REVIEWS
(2023)
